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  • 97
    Millipore brefeldin a
    Brefeldin A, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2022-10
    97/100 stars
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    99
    Becton Dickinson brefeldin a
    Microscopic assessment of cytokine production in iNKT cells cocultured with DCs from HIV-1 81A-eGFP-infected cultures. (A) Schematic representation of the assay. (B) α-GalCer-loaded DCs from a DC culture infected with HIV-1 81A-eGFP for 6 days were cocultured with iNKT cells for 2 h in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; eGFP, green; DAPI, blue; DCs, white circles; IFN-γ-positive iNKT cells in DC contact, *; original scale bar, 20 μm. (C) Quantification of iNKT cell IFNγ production after coculture with HIV-1 81A-eGFP or mock-infected DC cultures. Data represent the average of three independent experiments ±  sem ; * P
    Brefeldin A, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/Becton Dickinson
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher brefeldin a
    Microscopic assessment of cytokine production in iNKT cells cocultured with DCs from HIV-1 81A-eGFP-infected cultures. (A) Schematic representation of the assay. (B) α-GalCer-loaded DCs from a DC culture infected with HIV-1 81A-eGFP for 6 days were cocultured with iNKT cells for 2 h in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; eGFP, green; DAPI, blue; DCs, white circles; IFN-γ-positive iNKT cells in DC contact, *; original scale bar, 20 μm. (C) Quantification of iNKT cell IFNγ production after coculture with HIV-1 81A-eGFP or mock-infected DC cultures. Data represent the average of three independent experiments ±  sem ; * P
    Brefeldin A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    99
    BioLegend brefeldin a
    Microscopic assessment of cytokine production in iNKT cells cocultured with DCs from HIV-1 81A-eGFP-infected cultures. (A) Schematic representation of the assay. (B) α-GalCer-loaded DCs from a DC culture infected with HIV-1 81A-eGFP for 6 days were cocultured with iNKT cells for 2 h in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; eGFP, green; DAPI, blue; DCs, white circles; IFN-γ-positive iNKT cells in DC contact, *; original scale bar, 20 μm. (C) Quantification of iNKT cell IFNγ production after coculture with HIV-1 81A-eGFP or mock-infected DC cultures. Data represent the average of three independent experiments ±  sem ; * P
    Brefeldin A, supplied by BioLegend, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/brefeldin a/product/BioLegend
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Microscopic assessment of cytokine production in iNKT cells cocultured with DCs from HIV-1 81A-eGFP-infected cultures. (A) Schematic representation of the assay. (B) α-GalCer-loaded DCs from a DC culture infected with HIV-1 81A-eGFP for 6 days were cocultured with iNKT cells for 2 h in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; eGFP, green; DAPI, blue; DCs, white circles; IFN-γ-positive iNKT cells in DC contact, *; original scale bar, 20 μm. (C) Quantification of iNKT cell IFNγ production after coculture with HIV-1 81A-eGFP or mock-infected DC cultures. Data represent the average of three independent experiments ±  sem ; * P

    Journal: Journal of Leukocyte Biology

    Article Title: Technical Advance: Measurement of iNKT cell responses at the single-cell level against rare HIV-1-infected dendritic cells in a mixed culture

    doi: 10.1189/jlb.1012489

    Figure Lengend Snippet: Microscopic assessment of cytokine production in iNKT cells cocultured with DCs from HIV-1 81A-eGFP-infected cultures. (A) Schematic representation of the assay. (B) α-GalCer-loaded DCs from a DC culture infected with HIV-1 81A-eGFP for 6 days were cocultured with iNKT cells for 2 h in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; eGFP, green; DAPI, blue; DCs, white circles; IFN-γ-positive iNKT cells in DC contact, *; original scale bar, 20 μm. (C) Quantification of iNKT cell IFNγ production after coculture with HIV-1 81A-eGFP or mock-infected DC cultures. Data represent the average of three independent experiments ± sem ; * P

    Article Snippet: After 6 h of coculture in the presence of brefeldin A (2 mg/mL; GolgiPlug; BD Biosciences), cells were surface-stained with anti-CD3 and anti-CD11c mAb to discriminate between DCs and iNKT cells.

    Techniques: Infection, Staining

    Low frequency of HIV-1 infection hampers detection of differences in IFN-γ production in iNKT cells cocultured with uninfected and infected DC cultures by flow cytometry. iNKT cells were cocultured with α-GalCer-loaded DCs from mock-, 81A-, or 81A-eGFP-infected cultures at a ratio of 2:1 in the presence of brefeldin A for 6 h. The frequency of IFN-γ-producing iNKT cells was determined by surface CD3 and intracellular cytokine staining and flow cytometry.

    Journal: Journal of Leukocyte Biology

    Article Title: Technical Advance: Measurement of iNKT cell responses at the single-cell level against rare HIV-1-infected dendritic cells in a mixed culture

    doi: 10.1189/jlb.1012489

    Figure Lengend Snippet: Low frequency of HIV-1 infection hampers detection of differences in IFN-γ production in iNKT cells cocultured with uninfected and infected DC cultures by flow cytometry. iNKT cells were cocultured with α-GalCer-loaded DCs from mock-, 81A-, or 81A-eGFP-infected cultures at a ratio of 2:1 in the presence of brefeldin A for 6 h. The frequency of IFN-γ-producing iNKT cells was determined by surface CD3 and intracellular cytokine staining and flow cytometry.

    Article Snippet: After 6 h of coculture in the presence of brefeldin A (2 mg/mL; GolgiPlug; BD Biosciences), cells were surface-stained with anti-CD3 and anti-CD11c mAb to discriminate between DCs and iNKT cells.

    Techniques: Infection, Flow Cytometry, Cytometry, Staining

    Complex formation and IFN-γ production in DC-iNKT cell cocultures.  (A) DCs and iNKT cells were cocultured for 30 min, attached to glass microscope slides by cytospin, followed by fixation and DAPI staining. DAPI, blue; original scale bar, 10 μm. (B) DCs were loaded with α-GalCer or left untreated and cocultured with iNKT cells for the indicated times in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; DAPI, blue; original scale bar, 50 μm.

    Journal: Journal of Leukocyte Biology

    Article Title: Technical Advance: Measurement of iNKT cell responses at the single-cell level against rare HIV-1-infected dendritic cells in a mixed culture

    doi: 10.1189/jlb.1012489

    Figure Lengend Snippet: Complex formation and IFN-γ production in DC-iNKT cell cocultures. (A) DCs and iNKT cells were cocultured for 30 min, attached to glass microscope slides by cytospin, followed by fixation and DAPI staining. DAPI, blue; original scale bar, 10 μm. (B) DCs were loaded with α-GalCer or left untreated and cocultured with iNKT cells for the indicated times in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; DAPI, blue; original scale bar, 50 μm.

    Article Snippet: After 6 h of coculture in the presence of brefeldin A (2 mg/mL; GolgiPlug; BD Biosciences), cells were surface-stained with anti-CD3 and anti-CD11c mAb to discriminate between DCs and iNKT cells.

    Techniques: Microscopy, Staining