Journal: Journal of Leukocyte Biology
Article Title: Technical Advance: Measurement of iNKT cell responses at the single-cell level against rare HIV-1-infected dendritic cells in a mixed culture
Figure Lengend Snippet: Complex formation and IFN-γ production in DC-iNKT cell cocultures. (A) DCs and iNKT cells were cocultured for 30 min, attached to glass microscope slides by cytospin, followed by fixation and DAPI staining. DAPI, blue; original scale bar, 10 μm. (B) DCs were loaded with α-GalCer or left untreated and cocultured with iNKT cells for the indicated times in the presence of brefeldin A. Following cytospinning, cells were fixed, permeabilized, and stained with anti-IFN-γ mAb and DAPI. IFN-γ, red; DAPI, blue; original scale bar, 50 μm.
Article Snippet: After 6 h of coculture in the presence of brefeldin A (2 mg/mL; GolgiPlug; BD Biosciences), cells were surface-stained with anti-CD3 and anti-CD11c mAb to discriminate between DCs and iNKT cells.
Techniques: Microscopy, Staining