Journal: PLoS Genetics

Article Title: Dek overexpression in murine epithelia increases overt esophageal squamous cell carcinoma incidence

doi: 10.1371/journal.pgen.1007227

Figure Lengend Snippet: Dek overexpression in 4NQO-treated mice leads to trends in increased cellular proliferation. ( A ) The water soluble carcinogen 4-nitroquinoline 1-oxide (4NQO) confers susceptibility to head and neck squamous cell carcinoma (HNSCC). At six weeks of age, mice were given 4NQO drinking water for 16 weeks in the presence or absence of dox chow. After 16 weeks, the mice were returned to normal water and were monitored until death or sacrifice at 45 weeks of age, or earlier if moribund. Prior to sacrifice, mice were injected with BrdU to measure proliferative differences in tissues. Abbreviations: time (T), weeks (wks). ( B-C ) Quantification of BrdU positive cells per millimeter of squamous epithelium in ( B ) normal and ( C ) 4NQO treated tongue and esophagus of Bi-L-Dek_K5-tTA ]. ( D ) Representative IHC images of increased BrdU incorporation in the tongue and esophagus of Bi-L-Dek_K5-tTA mice treated with 4NQO compared to those on dox.

Article Snippet: Antibody dilutions were used as follows: BrdU (1:100, Invitrogen, Calsbad, CA, USA), and DEK (1:200, BD Biosciences, San Jose, CA, USA; or 1:300, Proteintech Group, Chicago, IL, USA; or 1:50, Cusabio, Baltimore, MD, USA).

Techniques: Over Expression, Mouse Assay, Injection, Immunohistochemistry, BrdU Incorporation Assay