brain heart infusion bhi medium Search Results


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  • 99
    Thermo Fisher brain heart infusion bhi medium
    Deletion of ArgR enhances survival of L. <t>monocytogenes</t> in the lethal acidic conditions. Overnight-grown L. monocytogenes wild-type and mutant strains were harvested, washed and then incubated in <t>BHI</t> broth (pre-adjusted to pH 3.5 using 3 M lactic acid, LA) at 37°C. Survivors were enumerated at regular intervals by plating serial dilutions on BHI plate. Data are expressed as Mean ± SD of recovery rate for each strain. ∗ P
    Brain Heart Infusion Bhi Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 292 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore brain heart infusion medium bhi
    Deletion of ArgR enhances survival of L. <t>monocytogenes</t> in the lethal acidic conditions. Overnight-grown L. monocytogenes wild-type and mutant strains were harvested, washed and then incubated in <t>BHI</t> broth (pre-adjusted to pH 3.5 using 3 M lactic acid, LA) at 37°C. Survivors were enumerated at regular intervals by plating serial dilutions on BHI plate. Data are expressed as Mean ± SD of recovery rate for each strain. ∗ P
    Brain Heart Infusion Medium Bhi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore brain heart infusion broth medium bhi
    Deletion of ArgR enhances survival of L. <t>monocytogenes</t> in the lethal acidic conditions. Overnight-grown L. monocytogenes wild-type and mutant strains were harvested, washed and then incubated in <t>BHI</t> broth (pre-adjusted to pH 3.5 using 3 M lactic acid, LA) at 37°C. Survivors were enumerated at regular intervals by plating serial dilutions on BHI plate. Data are expressed as Mean ± SD of recovery rate for each strain. ∗ P
    Brain Heart Infusion Broth Medium Bhi, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore brain heart infusion bhi medium
    Impaired growth of S. pneumoniae R6 strain EL1387 containing a regulated replacement of clpX (Δ clpX P fcsK :: clpX + ) on medium lacking fucose. (A) <t>EL59</t> ( clpX + parent), EL1383 (regulated merodiploid clpX + P fcsK :: clpX + ), and EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) were streaked onto TSA-BA plates containing 0.25% (wt/vol) fucose (inducing) or lacking additional fucose (noninducing). Plates were photographed after 24 h of incubation at 37°C in an atmosphere of 5% CO 2 . (B) EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) was grown statically overnight in <t>BHI</t> broth containing 0.1% (wt/vol) fucose at 37°C in 5% CO 2 . Cultures were then diluted 100-fold into fresh BHI containing or lacking 0.1% (wt/vol) fucose, and static incubation was continued at 37°C in 5% CO 2 . Filled circles and squares represent the optical densities and viable cell counts, respectively, of the culture containing 0.1% (wt/vol) fucose. Open circles and squares represent the optical densities and viable cell counts, respectively, of the culture lacking fucose (0.001% [wt/vol]) carryover fucose from starting culture). Results are representative of at least two independent experiments. OD 620 (1.4 cm), optical density at 620 nm for a tube with a 1.4-cm diameter.
    Brain Heart Infusion Bhi Medium, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Difco brain heart infusion bhi medium
    Role of the c-di-GMP-induced EPS in biofilm formation, cell aggregation, and tolerance of L. <t>monocytogenes</t> to disinfectants and desiccation. A : Biofilm formation of L. monocytogenes in 96-well polystyrene plates (measured using a Crystal violet dye-binding assay). Cultures were grown for 6 days at 30°C in LB (top panel) or LB supplemented with 3% glycerol (bottom panel). Shown are average results from two biological replicates, where each strain was grown in six wells in a replicate (i.e., six technical replicates). Black circle, wild type; red square, Δ pdeB/C/D ; green triangle, Δ pdeB/C/D Δ pssC ; blue cross, Δ pdeB/C/D Δ pssE . B : EPS-dependent L. monocytogenes cell aggregation (clumping) in HTM medium. Overnight cultures grown in <t>BHI</t> were inoculated into HTM liquid medium at A 600 of 0.01 and incubated at 30°C with gentle shaking (rotary shaker, 125 rpm) for 48 h. 1, ΔpdeB/C/D ; 2, wild type; 3, ΔpdeB/C/D ΔpssC ; 4, ΔpdeB/C/D ΔpssE ; C : Protective role of the c-di-GMP-inducible EPS in disinfection. Aliquots of the HTM-grown cultures were mixed with disinfectant solutions for 10 min at room temperature. Disinfection was stopped by adding a D/E neutralizing broth (Difco); the cultures were vortexed vigorously (5 min) with glass beads to break clumps and plated on BHI agar. Colonies were enumerated after a 48-h growth at 37°C. SH, sodium hydrochloride (1600 ppm); HP, hydrogen peroxide (200 mM); BC, benzalkonium chloride (100 ppm). White background, EGD-e; black, ΔpdeB/C/D ; grey, ΔpdeB/C/D ΔpssC . SH, sodium hypochlorite; HP, hydrogen peroxide; BC, benzalkonium chloride. The absence of the bar for the EGD-e strain treated with SH indicates the lack of survivors. D : Protective role of the c-di-GMP-inducible EPS in desiccation. Aliquots of overnight cultures grown in HTM at 37°C were spun down, the supernatants were removed, and cell pellets were stored in desiccators at room temperature for the indicated periods. The pellets were rehydrated, vortexed with glass beads for better suspension and plated on BHI agar. The numbers of surviving colonies after incubation at 37°C for 24 h are plotted. In panels C and D, bars denote mean values for data from three biological replicates. *, significantly different (p
    Brain Heart Infusion Bhi Medium, supplied by Difco, used in various techniques. Bioz Stars score: 98/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 289 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
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    Carl Roth GmbH brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Dot Scientific brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Dot Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hardy Diagnostics brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nissui Pharmaceutical brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Nissui Pharmaceutical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Forma Therapeutics brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Forma Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eiken Chemical brain heart infusion bhi medium
    Supplemental sugar alters S. <t>mutans</t> growth. A growth curve of S. mutans in <t>BHI</t> with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error
    Brain Heart Infusion Bhi Medium, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore brain heart infusion bhi broth
    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in <t>BHI</t> with serum at <t>37°C</t> and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)
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    Becton Dickinson brain heart infusion bhi
    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in <t>BHI</t> with serum at <t>37°C</t> and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)
    Brain Heart Infusion Bhi, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher medium brain heart infusion bhi broth
    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in <t>BHI</t> with serum at <t>37°C</t> and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)
    Medium Brain Heart Infusion Bhi Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco brain heart infusion bhi
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Brain Heart Infusion Bhi, supplied by Difco, used in various techniques. Bioz Stars score: 96/100, based on 468 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco brain heart infusion bhi transport medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Brain Heart Infusion Bhi Transport Medium, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson liquid brain heart infusion bhi medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Liquid Brain Heart Infusion Bhi Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co brain heart infusion bhi broth medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Brain Heart Infusion Bhi Broth Medium, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco semisolid brain heart infusion bhi medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Semisolid Brain Heart Infusion Bhi Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco bacto brain heart infusion bhi medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Bacto Brain Heart Infusion Bhi Medium, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson bacto brain heart infusion bhi medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Bacto Brain Heart Infusion Bhi Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories brain heart infusion bhi broth medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Brain Heart Infusion Bhi Broth Medium, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco brain heart infusion bhi liquid medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
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    Becton Dickinson brain heart infusion bhi broth medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Brain Heart Infusion Bhi Broth Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Teknova brain heart infusion bhi medium powder
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
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    Difco brain heart infusion bhi broth medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Brain Heart Infusion Bhi Broth Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco filter sterilized brain heart infusion bhi medium
    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich <t>FAG</t> and <t>BHI</t> media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots
    Filter Sterilized Brain Heart Infusion Bhi Medium, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Deletion of ArgR enhances survival of L. monocytogenes in the lethal acidic conditions. Overnight-grown L. monocytogenes wild-type and mutant strains were harvested, washed and then incubated in BHI broth (pre-adjusted to pH 3.5 using 3 M lactic acid, LA) at 37°C. Survivors were enumerated at regular intervals by plating serial dilutions on BHI plate. Data are expressed as Mean ± SD of recovery rate for each strain. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: Listeria monocytogenes 10403S Arginine Repressor ArgR Finely Tunes Arginine Metabolism Regulation under Acidic Conditions

    doi: 10.3389/fmicb.2017.00145

    Figure Lengend Snippet: Deletion of ArgR enhances survival of L. monocytogenes in the lethal acidic conditions. Overnight-grown L. monocytogenes wild-type and mutant strains were harvested, washed and then incubated in BHI broth (pre-adjusted to pH 3.5 using 3 M lactic acid, LA) at 37°C. Survivors were enumerated at regular intervals by plating serial dilutions on BHI plate. Data are expressed as Mean ± SD of recovery rate for each strain. ∗ P

    Article Snippet: L. monocytogenes was cultured in brain heart infusion (BHI) medium (Oxoid, Hampshire, England).

    Techniques: Mutagenesis, Incubation

    Growth of wild-type L. monocytogenes EGD ( filled circle ), Δ axyR mutant ( filled diagonal ), Δ phoP mutant ( filled square ) and Δ fri mutant ( filled triangle ) in BHI broth pH 5 adjusted with HCl ( a ) and in BHI broth supplemented with 0.5 M KCl ( b ). Overnight cultures were inoculated (1:100) into BHI broth supplemented with appropriate stressor, and cultures were incubated with shaking at 37 °C. Cell growth was measured spectrophotometrically by determining the optical density at 600 nm. Error bars , standard deviations from three independent experiments

    Journal: Archives of Microbiology

    Article Title: An essential role of a ferritin-like protein in acid stress tolerance of Listeria monocytogenes

    doi: 10.1007/s00203-014-1053-4

    Figure Lengend Snippet: Growth of wild-type L. monocytogenes EGD ( filled circle ), Δ axyR mutant ( filled diagonal ), Δ phoP mutant ( filled square ) and Δ fri mutant ( filled triangle ) in BHI broth pH 5 adjusted with HCl ( a ) and in BHI broth supplemented with 0.5 M KCl ( b ). Overnight cultures were inoculated (1:100) into BHI broth supplemented with appropriate stressor, and cultures were incubated with shaking at 37 °C. Cell growth was measured spectrophotometrically by determining the optical density at 600 nm. Error bars , standard deviations from three independent experiments

    Article Snippet: L. monocytogenes strains were grown in brain heart infusion (BHI) broth medium (Oxoid).

    Techniques: Mutagenesis, Incubation

    Transcriptional analysis of gene expression under temperature, acid and osmotic stress conditions using RT-PCR. Total RNA was isolated from exponential phase cultures of L. monocytogenes EGD strain grown in BHI at 37 °C ( 1 ) and exposed to 42 °C ( 2 ) or pH 5 ( 3 ) or 0.5 M KCl ( 4 ) for 90 min in each case. All RT-PCRs were performed three times from three separate RNA preparations. In all cases, control PCR were performed to ensure the complete removal of DNA from RNA preparations prior to reverse transcription

    Journal: Archives of Microbiology

    Article Title: An essential role of a ferritin-like protein in acid stress tolerance of Listeria monocytogenes

    doi: 10.1007/s00203-014-1053-4

    Figure Lengend Snippet: Transcriptional analysis of gene expression under temperature, acid and osmotic stress conditions using RT-PCR. Total RNA was isolated from exponential phase cultures of L. monocytogenes EGD strain grown in BHI at 37 °C ( 1 ) and exposed to 42 °C ( 2 ) or pH 5 ( 3 ) or 0.5 M KCl ( 4 ) for 90 min in each case. All RT-PCRs were performed three times from three separate RNA preparations. In all cases, control PCR were performed to ensure the complete removal of DNA from RNA preparations prior to reverse transcription

    Article Snippet: L. monocytogenes strains were grown in brain heart infusion (BHI) broth medium (Oxoid).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Polymerase Chain Reaction

    Impaired growth of S. pneumoniae R6 strain EL1387 containing a regulated replacement of clpX (Δ clpX P fcsK :: clpX + ) on medium lacking fucose. (A) EL59 ( clpX + parent), EL1383 (regulated merodiploid clpX + P fcsK :: clpX + ), and EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) were streaked onto TSA-BA plates containing 0.25% (wt/vol) fucose (inducing) or lacking additional fucose (noninducing). Plates were photographed after 24 h of incubation at 37°C in an atmosphere of 5% CO 2 . (B) EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) was grown statically overnight in BHI broth containing 0.1% (wt/vol) fucose at 37°C in 5% CO 2 . Cultures were then diluted 100-fold into fresh BHI containing or lacking 0.1% (wt/vol) fucose, and static incubation was continued at 37°C in 5% CO 2 . Filled circles and squares represent the optical densities and viable cell counts, respectively, of the culture containing 0.1% (wt/vol) fucose. Open circles and squares represent the optical densities and viable cell counts, respectively, of the culture lacking fucose (0.001% [wt/vol]) carryover fucose from starting culture). Results are representative of at least two independent experiments. OD 620 (1.4 cm), optical density at 620 nm for a tube with a 1.4-cm diameter.

    Journal: Journal of Bacteriology

    Article Title: Essentiality of clpX, but Not clpP, clpL, clpC, or clpE, in Streptococcus pneumoniae R6

    doi: 10.1128/JB.185.9.2961-2966.2003

    Figure Lengend Snippet: Impaired growth of S. pneumoniae R6 strain EL1387 containing a regulated replacement of clpX (Δ clpX P fcsK :: clpX + ) on medium lacking fucose. (A) EL59 ( clpX + parent), EL1383 (regulated merodiploid clpX + P fcsK :: clpX + ), and EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) were streaked onto TSA-BA plates containing 0.25% (wt/vol) fucose (inducing) or lacking additional fucose (noninducing). Plates were photographed after 24 h of incubation at 37°C in an atmosphere of 5% CO 2 . (B) EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) was grown statically overnight in BHI broth containing 0.1% (wt/vol) fucose at 37°C in 5% CO 2 . Cultures were then diluted 100-fold into fresh BHI containing or lacking 0.1% (wt/vol) fucose, and static incubation was continued at 37°C in 5% CO 2 . Filled circles and squares represent the optical densities and viable cell counts, respectively, of the culture containing 0.1% (wt/vol) fucose. Open circles and squares represent the optical densities and viable cell counts, respectively, of the culture lacking fucose (0.001% [wt/vol]) carryover fucose from starting culture). Results are representative of at least two independent experiments. OD 620 (1.4 cm), optical density at 620 nm for a tube with a 1.4-cm diameter.

    Article Snippet: Ten independent exponential cultures of EL59 (R6 parent strain) in brain heart infusion (BHI) medium were diluted 1:20 in 1 ml of BHI medium containing 10% heat-inactivated horse serum (Sigma), 10 mM glucose, and 100 ng of competence stimulatory peptide 1 to give a cell density of ∼7.0 × 105 CFU/ml ( ).

    Techniques: Incubation

    Role of the c-di-GMP-induced EPS in biofilm formation, cell aggregation, and tolerance of L. monocytogenes to disinfectants and desiccation. A : Biofilm formation of L. monocytogenes in 96-well polystyrene plates (measured using a Crystal violet dye-binding assay). Cultures were grown for 6 days at 30°C in LB (top panel) or LB supplemented with 3% glycerol (bottom panel). Shown are average results from two biological replicates, where each strain was grown in six wells in a replicate (i.e., six technical replicates). Black circle, wild type; red square, Δ pdeB/C/D ; green triangle, Δ pdeB/C/D Δ pssC ; blue cross, Δ pdeB/C/D Δ pssE . B : EPS-dependent L. monocytogenes cell aggregation (clumping) in HTM medium. Overnight cultures grown in BHI were inoculated into HTM liquid medium at A 600 of 0.01 and incubated at 30°C with gentle shaking (rotary shaker, 125 rpm) for 48 h. 1, ΔpdeB/C/D ; 2, wild type; 3, ΔpdeB/C/D ΔpssC ; 4, ΔpdeB/C/D ΔpssE ; C : Protective role of the c-di-GMP-inducible EPS in disinfection. Aliquots of the HTM-grown cultures were mixed with disinfectant solutions for 10 min at room temperature. Disinfection was stopped by adding a D/E neutralizing broth (Difco); the cultures were vortexed vigorously (5 min) with glass beads to break clumps and plated on BHI agar. Colonies were enumerated after a 48-h growth at 37°C. SH, sodium hydrochloride (1600 ppm); HP, hydrogen peroxide (200 mM); BC, benzalkonium chloride (100 ppm). White background, EGD-e; black, ΔpdeB/C/D ; grey, ΔpdeB/C/D ΔpssC . SH, sodium hypochlorite; HP, hydrogen peroxide; BC, benzalkonium chloride. The absence of the bar for the EGD-e strain treated with SH indicates the lack of survivors. D : Protective role of the c-di-GMP-inducible EPS in desiccation. Aliquots of overnight cultures grown in HTM at 37°C were spun down, the supernatants were removed, and cell pellets were stored in desiccators at room temperature for the indicated periods. The pellets were rehydrated, vortexed with glass beads for better suspension and plated on BHI agar. The numbers of surviving colonies after incubation at 37°C for 24 h are plotted. In panels C and D, bars denote mean values for data from three biological replicates. *, significantly different (p

    Journal: PLoS Pathogens

    Article Title: Cyclic di-GMP-dependent Signaling Pathways in the Pathogenic Firmicute Listeria monocytogenes

    doi: 10.1371/journal.ppat.1004301

    Figure Lengend Snippet: Role of the c-di-GMP-induced EPS in biofilm formation, cell aggregation, and tolerance of L. monocytogenes to disinfectants and desiccation. A : Biofilm formation of L. monocytogenes in 96-well polystyrene plates (measured using a Crystal violet dye-binding assay). Cultures were grown for 6 days at 30°C in LB (top panel) or LB supplemented with 3% glycerol (bottom panel). Shown are average results from two biological replicates, where each strain was grown in six wells in a replicate (i.e., six technical replicates). Black circle, wild type; red square, Δ pdeB/C/D ; green triangle, Δ pdeB/C/D Δ pssC ; blue cross, Δ pdeB/C/D Δ pssE . B : EPS-dependent L. monocytogenes cell aggregation (clumping) in HTM medium. Overnight cultures grown in BHI were inoculated into HTM liquid medium at A 600 of 0.01 and incubated at 30°C with gentle shaking (rotary shaker, 125 rpm) for 48 h. 1, ΔpdeB/C/D ; 2, wild type; 3, ΔpdeB/C/D ΔpssC ; 4, ΔpdeB/C/D ΔpssE ; C : Protective role of the c-di-GMP-inducible EPS in disinfection. Aliquots of the HTM-grown cultures were mixed with disinfectant solutions for 10 min at room temperature. Disinfection was stopped by adding a D/E neutralizing broth (Difco); the cultures were vortexed vigorously (5 min) with glass beads to break clumps and plated on BHI agar. Colonies were enumerated after a 48-h growth at 37°C. SH, sodium hydrochloride (1600 ppm); HP, hydrogen peroxide (200 mM); BC, benzalkonium chloride (100 ppm). White background, EGD-e; black, ΔpdeB/C/D ; grey, ΔpdeB/C/D ΔpssC . SH, sodium hypochlorite; HP, hydrogen peroxide; BC, benzalkonium chloride. The absence of the bar for the EGD-e strain treated with SH indicates the lack of survivors. D : Protective role of the c-di-GMP-inducible EPS in desiccation. Aliquots of overnight cultures grown in HTM at 37°C were spun down, the supernatants were removed, and cell pellets were stored in desiccators at room temperature for the indicated periods. The pellets were rehydrated, vortexed with glass beads for better suspension and plated on BHI agar. The numbers of surviving colonies after incubation at 37°C for 24 h are plotted. In panels C and D, bars denote mean values for data from three biological replicates. *, significantly different (p

    Article Snippet: L. monocytogenes was grown in Brain Heart Infusion (BHI) medium (Difco), HTM (minimal medium containing 3% glucose) or LB, supplemented with appropriate antibiotics at 25, 30, 37 or 42°C, as indicated.

    Techniques: Binding Assay, Incubation

    In vitro characterization of mutant strains 10403S Δ virR and 10403S Δ virAB . (A) Growth of 10403S-derived strains in BHI broth. Bacterial cultures were grown with shaking at 37°C for 7 h, and the OD 600 was measured at 30-min intervals. Data are averages ± standard deviations for three experiments. (B) Intracellular growth in L2 murine fibroblasts. L2 fibroblasts were infected with the indicated strains. At 2-h intervals postinfection, L2 cells were lysed, and bacteria were enumerated by plating dilutions of lysates. Data are averages ± standard deviations for three experiments performed in duplicate. (C to E) Association of intracellular L. monocytogenes with F-actin and actin tails. L2 fibroblasts infected with the indicated strains were fixed, permeabilized, stained with anti- L. monocytogenes antibodies (red), phalloidin (green), and DAPI (blue), and imaged using a confocal microscope. Representative confocal images of infected L2 fibroblasts are shown. (F) Actin tail lengths. Data represent > 75 measurements per strain; the mean tail length for each strain is indicated by a horizontal bar. Bacteria without actin tails were not counted. Asterisks indicate significant differences from 10403S as determined by one-way ANOVA with a post hoc Tukey multiple-comparison test ( P

    Journal: Infection and Immunity

    Article Title: The VirAB ABC Transporter Is Required for VirR Regulation of Listeria monocytogenes Virulence and Resistance to Nisin

    doi: 10.1128/IAI.00901-17

    Figure Lengend Snippet: In vitro characterization of mutant strains 10403S Δ virR and 10403S Δ virAB . (A) Growth of 10403S-derived strains in BHI broth. Bacterial cultures were grown with shaking at 37°C for 7 h, and the OD 600 was measured at 30-min intervals. Data are averages ± standard deviations for three experiments. (B) Intracellular growth in L2 murine fibroblasts. L2 fibroblasts were infected with the indicated strains. At 2-h intervals postinfection, L2 cells were lysed, and bacteria were enumerated by plating dilutions of lysates. Data are averages ± standard deviations for three experiments performed in duplicate. (C to E) Association of intracellular L. monocytogenes with F-actin and actin tails. L2 fibroblasts infected with the indicated strains were fixed, permeabilized, stained with anti- L. monocytogenes antibodies (red), phalloidin (green), and DAPI (blue), and imaged using a confocal microscope. Representative confocal images of infected L2 fibroblasts are shown. (F) Actin tail lengths. Data represent > 75 measurements per strain; the mean tail length for each strain is indicated by a horizontal bar. Bacteria without actin tails were not counted. Asterisks indicate significant differences from 10403S as determined by one-way ANOVA with a post hoc Tukey multiple-comparison test ( P

    Article Snippet: L. monocytogenes strains were grown in brain heart infusion medium (BHI) (Difco, Detroit, MI) or LB broth where indicated.

    Techniques: In Vitro, Mutagenesis, Derivative Assay, Infection, Staining, Microscopy

    Kinetics of doxercalciferol and bacitracin interaction in MX804 (red), ΔmbrA (blue), and mbrA + (green) strains. Cultures of S. mutans MX804, Δ mbrA , and mbrA + were grown in TY medium plus 1% (wt/vol) glucose to exponential phase, and 10 5 CFU was used to inoculate fresh medium. The cultures were serially diluted and plated on BHI agar medium. Drugs were then added to the cultures as follows: doxercalciferol (16 μg/ml) (A), bacitracin (32 μg/ml) (B), a combination of doxercalciferol (16 μg/ml) and bacitracin (32 μg/ml) (C), or no-drug control (D). Aliquots were removed at 2, 4, 24, and 48 h following addition of drug and plated for enumeration. Percent survival was calculated by enumeration of CFU per milliliter at each time point. The data are averages of at least 3 independent replicates and are normalized to CFU per milliliter at time zero for each strain. *, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Vitamin D Compounds Are Bactericidal against Streptococcus mutans and Target the Bacitracin-Associated Efflux System

    doi: 10.1128/AAC.01675-17

    Figure Lengend Snippet: Kinetics of doxercalciferol and bacitracin interaction in MX804 (red), ΔmbrA (blue), and mbrA + (green) strains. Cultures of S. mutans MX804, Δ mbrA , and mbrA + were grown in TY medium plus 1% (wt/vol) glucose to exponential phase, and 10 5 CFU was used to inoculate fresh medium. The cultures were serially diluted and plated on BHI agar medium. Drugs were then added to the cultures as follows: doxercalciferol (16 μg/ml) (A), bacitracin (32 μg/ml) (B), a combination of doxercalciferol (16 μg/ml) and bacitracin (32 μg/ml) (C), or no-drug control (D). Aliquots were removed at 2, 4, 24, and 48 h following addition of drug and plated for enumeration. Percent survival was calculated by enumeration of CFU per milliliter at each time point. The data are averages of at least 3 independent replicates and are normalized to CFU per milliliter at time zero for each strain. *, P

    Article Snippet: S. mutans strain UA159 ( ) was maintained on brain heart infusion (BHI) agar medium (BD/Difco, Franklin Lakes, NJ).

    Techniques:

    Supplemental sugar alters S. mutans growth. A growth curve of S. mutans in BHI with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error

    Journal: Applied and Environmental Microbiology

    Article Title: Salivary Mucins Protect Surfaces from Colonization by Cariogenic Bacteria

    doi: 10.1128/AEM.02573-14

    Figure Lengend Snippet: Supplemental sugar alters S. mutans growth. A growth curve of S. mutans in BHI with added 1% glucose (Medium+Glucose) or 1% sucrose (Medium+Sucrose) shows that S. mutans ' growth rate changes based on the specific sugar present in the growth medium. Error

    Article Snippet: For sucrose and glucose experiments, S. mutans was grown overnight in brain heart infusion (BHI) medium (Becton, Dickinson and Company) containing 1% (wt/vol) sucrose and BHI with 1% (wt/vol) glucose (Sigma).

    Techniques:

    Salivary mucins reduce S. mutans attachment and biofilm formation. The addition of 0.3% mucins to the control medium, BHI containing 1% sucrose (SMedium), significantly reduces the levels of S. mutans attachment and biofilm formation on glass (A, B) and

    Journal: Applied and Environmental Microbiology

    Article Title: Salivary Mucins Protect Surfaces from Colonization by Cariogenic Bacteria

    doi: 10.1128/AEM.02573-14

    Figure Lengend Snippet: Salivary mucins reduce S. mutans attachment and biofilm formation. The addition of 0.3% mucins to the control medium, BHI containing 1% sucrose (SMedium), significantly reduces the levels of S. mutans attachment and biofilm formation on glass (A, B) and

    Article Snippet: For sucrose and glucose experiments, S. mutans was grown overnight in brain heart infusion (BHI) medium (Becton, Dickinson and Company) containing 1% (wt/vol) sucrose and BHI with 1% (wt/vol) glucose (Sigma).

    Techniques:

    S. mutans growth is unaffected by the presence of salivary mucins. A growth curve of S. mutans in BHI with 1% sucrose (SMedium), BHI with 1% sucrose and 0.3% mucins, or BHI with 1% sucrose and 0.3% methylcellulose indicates that the presence of mucins

    Journal: Applied and Environmental Microbiology

    Article Title: Salivary Mucins Protect Surfaces from Colonization by Cariogenic Bacteria

    doi: 10.1128/AEM.02573-14

    Figure Lengend Snippet: S. mutans growth is unaffected by the presence of salivary mucins. A growth curve of S. mutans in BHI with 1% sucrose (SMedium), BHI with 1% sucrose and 0.3% mucins, or BHI with 1% sucrose and 0.3% methylcellulose indicates that the presence of mucins

    Article Snippet: For sucrose and glucose experiments, S. mutans was grown overnight in brain heart infusion (BHI) medium (Becton, Dickinson and Company) containing 1% (wt/vol) sucrose and BHI with 1% (wt/vol) glucose (Sigma).

    Techniques:

    Sucrose enhances S. mutans attachment and biofilm formation. The levels of S. mutans attachment (A) and biofilm formation (B) on glass are significantly enhanced at all time points when the bacteria are grown in BHI containing 1% sucrose (Medium+Sucrose)

    Journal: Applied and Environmental Microbiology

    Article Title: Salivary Mucins Protect Surfaces from Colonization by Cariogenic Bacteria

    doi: 10.1128/AEM.02573-14

    Figure Lengend Snippet: Sucrose enhances S. mutans attachment and biofilm formation. The levels of S. mutans attachment (A) and biofilm formation (B) on glass are significantly enhanced at all time points when the bacteria are grown in BHI containing 1% sucrose (Medium+Sucrose)

    Article Snippet: For sucrose and glucose experiments, S. mutans was grown overnight in brain heart infusion (BHI) medium (Becton, Dickinson and Company) containing 1% (wt/vol) sucrose and BHI with 1% (wt/vol) glucose (Sigma).

    Techniques:

    S. mutans survival is unaffected by salivary mucins. The graph represents the total number of viable S. mutans cells per well in the supernatant and biofilm in BHI with 1% sucrose (SMedium), BHI with 1% sucrose and 0.3% mucins, or BHI with 1% sucrose

    Journal: Applied and Environmental Microbiology

    Article Title: Salivary Mucins Protect Surfaces from Colonization by Cariogenic Bacteria

    doi: 10.1128/AEM.02573-14

    Figure Lengend Snippet: S. mutans survival is unaffected by salivary mucins. The graph represents the total number of viable S. mutans cells per well in the supernatant and biofilm in BHI with 1% sucrose (SMedium), BHI with 1% sucrose and 0.3% mucins, or BHI with 1% sucrose

    Article Snippet: For sucrose and glucose experiments, S. mutans was grown overnight in brain heart infusion (BHI) medium (Becton, Dickinson and Company) containing 1% (wt/vol) sucrose and BHI with 1% (wt/vol) glucose (Sigma).

    Techniques:

    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in BHI with serum at 37°C and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)

    Journal: FEMS microbiology letters

    Article Title: Expression of the Collagen Adhesin ace by Enterococcus faecalis Strain OG1RF is not Repressed by Ers but Requires the Ers box

    doi: 10.1111/1574-6968.12146

    Figure Lengend Snippet: Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in BHI with serum at 37°C and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)

    Article Snippet: Bacteria were grown at 37°C in Luria-Bertani (LB) broth, Brain Heart Infusion (BHI) broth, M17 medium supplemented with 0.5% glucose (M17-G) or MM9YEG with 10 mM p -chloro-phenylalanine (p-Cl-Phe, Sigma-Aldrich Co.) ( ).

    Techniques: Expressing, Northern Blot

    FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Journal: Applied and Environmental Microbiology

    Article Title: A Single Mechanosensitive Channel Protects Francisella tularensis subsp. holarctica from Hypoosmotic Shock and Promotes Survival in the Aquatic Environment

    doi: 10.1128/AEM.02203-17

    Figure Lengend Snippet: FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Article Snippet: For downshock experiments conducted in medium, single colonies of each strain were picked from MH chocolate agar plates and grown in BHI broth supplemented with 300 mM NaCl (EMD Chemicals).

    Techniques:

    2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich FAG and BHI media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots

    Journal: Journal of Bacteriology

    Article Title: Differential Proteomic Analysis of the Bacillus anthracis Secretome: Distinct Plasmid and Chromosome CO2-Dependent Cross Talk Mechanisms Modulate Extracellular Proteolytic Activities †

    doi: 10.1128/JB.188.10.3551-3571.2006

    Figure Lengend Snippet: 2-DE separation of proteins secreted by B. anthracis strain Vollum cultured in rich FAG and BHI media. The numbers in the left lower corners of the panels correspond to those in Fig. . For a detailed identification of the various spots

    Article Snippet: Cells were cultured in either FAG medium , brain heart infusion (BHI) (Difco/Becton Dickinson, MD), or NBY medium (containing 0.8% [w/vol] nutrient broth [Difco], 0.3% yeast extract [Difco], and 0.5% glucose) for up to 24 h at 37°C with vigorous agitation.

    Techniques: Cell Culture