brain heart infusion bhi broth Search Results


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  • 99
    Thermo Fisher brain heart infusion bhi broth
    Detection of E. faecalis EbpC on the cell surface by immunoelectron microscopy. Anti-EbpC MAb-probed <t>OG1RF</t> cells grown in <t>BHI</t> were immunogold labeled, negatively stained with 1% uranyl acetate, and viewed by transmission electron microscopy.
    Brain Heart Infusion Bhi Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore brain heart infusion bhi broth
    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in <t>BHI</t> with serum at <t>37°C</t> and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)
    Brain Heart Infusion Bhi Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad brain heart infusion broth bhi
    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in <t>BHI</t> with serum at <t>37°C</t> and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)
    Brain Heart Infusion Broth Bhi, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher brain heart infusion broth
    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in <t>BHI</t> with serum at <t>37°C</t> and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)
    Brain Heart Infusion Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore brain heart infusion broth medium bhi
    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in <t>BHI</t> with serum at <t>37°C</t> and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)
    Brain Heart Infusion Broth Medium Bhi, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Difco brain heart infusion bhi broth
    Protease activation in the extracellular protein fraction from P. <t>gingivalis</t> FLL93. P. gingivalis was grown to stationary phase (OD 600 of 1.3) in 1 liter of <t>BHI</t> broth supplemented with hemin and vitamin K. Acetone (37.5%)-precipitated proteins were mixed with 8 M urea and were incubated at 4°C for 1 h. The urea was slowly removed from the mixture by centrifugation (10,000 × g ) in a Millipore filtration unit with the addition of increasing volumes of 100 mM Tris-HCl buffer (pH 7.4). Activities for Rgp and Kgp were tested. Error bars indicate the standard errors of the means of three independent trials.
    Brain Heart Infusion Bhi Broth, supplied by Difco, used in various techniques. Bioz Stars score: 99/100, based on 1283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher in on brain heart infusion bhi broth agar
    Protease activation in the extracellular protein fraction from P. <t>gingivalis</t> FLL93. P. gingivalis was grown to stationary phase (OD 600 of 1.3) in 1 liter of <t>BHI</t> broth supplemented with hemin and vitamin K. Acetone (37.5%)-precipitated proteins were mixed with 8 M urea and were incubated at 4°C for 1 h. The urea was slowly removed from the mixture by centrifugation (10,000 × g ) in a Millipore filtration unit with the addition of increasing volumes of 100 mM Tris-HCl buffer (pH 7.4). Activities for Rgp and Kgp were tested. Error bars indicate the standard errors of the means of three independent trials.
    In On Brain Heart Infusion Bhi Broth Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson brain heart infusion bhi broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Bhi Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 873 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA brain heart infusion bhi broth
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> <t>BHI,</t> brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Brain Heart Infusion Bhi Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 99/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories brain heart infusion broth bhi
    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, <t>Luria-Bertani;</t> <t>BHI,</t> brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.
    Brain Heart Infusion Broth Bhi, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Merck & Co brain heart infusion bhi broth
    Correlation of bioluminescence (relative light units [RLU]) and culture regimen-dependent viable cell count (CFU per square centimeter) measured at <t>10°C.</t> The A lines show the correlation on cheese after 14 days of storage (open squares) and during the first 3 days after inoculation (closed squares). The B lines show the correlation for Y . enterocolitica on <t>BHI</t> agar with 2.5% salt (closed triangles) and without salt (open triangles) on the first 5 days. The background luminescence of cheese and BHI agar has been subtracted. Immediately after the light intensity was measured, the plates or cheeses were used to determine the viable counts (see text).
    Brain Heart Infusion Bhi Broth, supplied by Merck & Co, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lab M Ltd brain heart infusion bhi broth
    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. <t>monocytogenes</t> EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in <t>BHI</t> from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl
    Brain Heart Infusion Bhi Broth, supplied by Lab M Ltd, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Fisher Scientific brain heart infusion bhi broth
    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. <t>monocytogenes</t> EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in <t>BHI</t> from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl
    Brain Heart Infusion Bhi Broth, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Roth GmbH brain heart infusion bhi broth
    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. <t>monocytogenes</t> EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in <t>BHI</t> from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl
    Brain Heart Infusion Bhi Broth, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Liofilchem brain heart infusion bhi broth
    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. <t>monocytogenes</t> EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in <t>BHI</t> from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl
    Brain Heart Infusion Bhi Broth, supplied by Liofilchem, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    BioLife Solutions brain heart infusion broth bhi
    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. <t>monocytogenes</t> EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in <t>BHI</t> from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl
    Brain Heart Infusion Broth Bhi, supplied by BioLife Solutions, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Hardy Diagnostics brain heart infusion bhi broth
    P. aeruginosa supports the growth of F. nucleatum in both planktonic and biofilm settings under aerobic conditions. Tubes containing 5 mL of <t>BHI</t> broth and a cellulose disk were inoculated with ~10 6 CFU of P. aeruginosa and 10 2 CFU of F. nucleatum and incubated aerobically at <t>37°C</t> for time indicated on graph. (a) The disks were removed and rinsed to remove planktonic bacteria, and the numbers of CFU in the biofilm (CFU/disk) were determined. (b) The numbers of planktonic bacteria (CFU/mL) were also determined. Values in (a) and (b) represent the means of three independent experiments ± SEM; ∗ P
    Brain Heart Infusion Bhi Broth, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 95/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HY Labs brain heart infusion bhi broth
    P. aeruginosa supports the growth of F. nucleatum in both planktonic and biofilm settings under aerobic conditions. Tubes containing 5 mL of <t>BHI</t> broth and a cellulose disk were inoculated with ~10 6 CFU of P. aeruginosa and 10 2 CFU of F. nucleatum and incubated aerobically at <t>37°C</t> for time indicated on graph. (a) The disks were removed and rinsed to remove planktonic bacteria, and the numbers of CFU in the biofilm (CFU/disk) were determined. (b) The numbers of planktonic bacteria (CFU/mL) were also determined. Values in (a) and (b) represent the means of three independent experiments ± SEM; ∗ P
    Brain Heart Infusion Bhi Broth, supplied by HY Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor brain heart infusion bhi broth
    Invasion of CPEK monolayers by S. <t>pseudintermedius</t> strains. S. pseudintermedius cells were incubated with CPEK monolayers. Extracellular bacteria were killed with gentamicin, and internalized bacteria were quantified by plating lysates on <t>BHI</t> agar. The assay was performed three times. Each point represents the average value for three replicas, and error bars show the standard deviations (SD). Statistically significant ( P
    Brain Heart Infusion Bhi Broth, supplied by Avantor, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eiken Chemical brain heart infusion bhi broth
    Production of cytolysins by L. monocytogenes mutants. Wild-type and mutant L. monocytogenes strains were cultured first in <t>BHI</t> medium and then transferred into RPMI 1640 medium. Three hours after cultivation, the supernatant was collected by centrifugation. (A) An aliquot of supernatant was applied to SDS-polyacrylamide gels and transferred onto a polyvinylidene difluoride membrane. Immunoblotting was performed with anti-LLO polyclonal antibody (left) or anti-ILO polyclonal antibody (right). (B) Culture supernatants were twofold serially diluted with PBS and mixed with an equal volume of a 2% suspension of SRBCs. After incubation for 30 min at <t>37°C,</t> the hemolytic activity was measured by the amount of hemoglobin released into the supernatant. One hundred percent hemolysis was defined as the level of hemoglobin released after treatment with 2% Triton X-100.
    Brain Heart Infusion Bhi Broth, supplied by Eiken Chemical, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Laboratorios Conda brain heart infusion bhi broth
    Production of cytolysins by L. monocytogenes mutants. Wild-type and mutant L. monocytogenes strains were cultured first in <t>BHI</t> medium and then transferred into RPMI 1640 medium. Three hours after cultivation, the supernatant was collected by centrifugation. (A) An aliquot of supernatant was applied to SDS-polyacrylamide gels and transferred onto a polyvinylidene difluoride membrane. Immunoblotting was performed with anti-LLO polyclonal antibody (left) or anti-ILO polyclonal antibody (right). (B) Culture supernatants were twofold serially diluted with PBS and mixed with an equal volume of a 2% suspension of SRBCs. After incubation for 30 min at <t>37°C,</t> the hemolytic activity was measured by the amount of hemoglobin released into the supernatant. One hundred percent hemolysis was defined as the level of hemoglobin released after treatment with 2% Triton X-100.
    Brain Heart Infusion Bhi Broth, supplied by Laboratorios Conda, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    scharlau brain heart infusion bhi broth
    Production of cytolysins by L. monocytogenes mutants. Wild-type and mutant L. monocytogenes strains were cultured first in <t>BHI</t> medium and then transferred into RPMI 1640 medium. Three hours after cultivation, the supernatant was collected by centrifugation. (A) An aliquot of supernatant was applied to SDS-polyacrylamide gels and transferred onto a polyvinylidene difluoride membrane. Immunoblotting was performed with anti-LLO polyclonal antibody (left) or anti-ILO polyclonal antibody (right). (B) Culture supernatants were twofold serially diluted with PBS and mixed with an equal volume of a 2% suspension of SRBCs. After incubation for 30 min at <t>37°C,</t> the hemolytic activity was measured by the amount of hemoglobin released into the supernatant. One hundred percent hemolysis was defined as the level of hemoglobin released after treatment with 2% Triton X-100.
    Brain Heart Infusion Bhi Broth, supplied by scharlau, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alpha Biosciences brain heart infusion bhi broth
    Production of cytolysins by L. monocytogenes mutants. Wild-type and mutant L. monocytogenes strains were cultured first in <t>BHI</t> medium and then transferred into RPMI 1640 medium. Three hours after cultivation, the supernatant was collected by centrifugation. (A) An aliquot of supernatant was applied to SDS-polyacrylamide gels and transferred onto a polyvinylidene difluoride membrane. Immunoblotting was performed with anti-LLO polyclonal antibody (left) or anti-ILO polyclonal antibody (right). (B) Culture supernatants were twofold serially diluted with PBS and mixed with an equal volume of a 2% suspension of SRBCs. After incubation for 30 min at <t>37°C,</t> the hemolytic activity was measured by the amount of hemoglobin released into the supernatant. One hundred percent hemolysis was defined as the level of hemoglobin released after treatment with 2% Triton X-100.
    Brain Heart Infusion Bhi Broth, supplied by Alpha Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson bbltm brain heart infusion bhi broth
    Production of cytolysins by L. monocytogenes mutants. Wild-type and mutant L. monocytogenes strains were cultured first in <t>BHI</t> medium and then transferred into RPMI 1640 medium. Three hours after cultivation, the supernatant was collected by centrifugation. (A) An aliquot of supernatant was applied to SDS-polyacrylamide gels and transferred onto a polyvinylidene difluoride membrane. Immunoblotting was performed with anti-LLO polyclonal antibody (left) or anti-ILO polyclonal antibody (right). (B) Culture supernatants were twofold serially diluted with PBS and mixed with an equal volume of a 2% suspension of SRBCs. After incubation for 30 min at <t>37°C,</t> the hemolytic activity was measured by the amount of hemoglobin released into the supernatant. One hundred percent hemolysis was defined as the level of hemoglobin released after treatment with 2% Triton X-100.
    Bbltm Brain Heart Infusion Bhi Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Detection of E. faecalis EbpC on the cell surface by immunoelectron microscopy. Anti-EbpC MAb-probed OG1RF cells grown in BHI were immunogold labeled, negatively stained with 1% uranyl acetate, and viewed by transmission electron microscopy.

    Journal: Infection and Immunity

    Article Title: Targeting Pili in Enterococcal Pathogenesis

    doi: 10.1128/IAI.01403-13

    Figure Lengend Snippet: Detection of E. faecalis EbpC on the cell surface by immunoelectron microscopy. Anti-EbpC MAb-probed OG1RF cells grown in BHI were immunogold labeled, negatively stained with 1% uranyl acetate, and viewed by transmission electron microscopy.

    Article Snippet: Briefly, overnight-cultured OG1RF was inoculated into 20 ml brain heart infusion (BHI) broth (Gibco) at a starting optical density at 600 nm (OD600 ) of 0.05 and harvested at mid-log phase.

    Techniques: Immuno-Electron Microscopy, Labeling, Staining, Transmission Assay, Electron Microscopy

    Oxidative stress sensitivity of a S. aureus Δ cymR mutant. Viability of SA6 (SH1000/pMK4), SA30 (Δ cymR /pMK4) and SA31 (Δ cymR /pDIA5780) was tested. Exponential-phase cells grown in TSB medium with 2 mM cystine were treated for 1 h with 20 mM H 2 O 2 and plated on BHI. Results represent the mean values for survival with standard deviations and are representative of at least two independent experiments.

    Journal: PLoS Pathogens

    Article Title: The Pleiotropic CymR Regulator of Staphylococcus aureus Plays an Important Role in Virulence and Stress Response

    doi: 10.1371/journal.ppat.1000894

    Figure Lengend Snippet: Oxidative stress sensitivity of a S. aureus Δ cymR mutant. Viability of SA6 (SH1000/pMK4), SA30 (Δ cymR /pMK4) and SA31 (Δ cymR /pDIA5780) was tested. Exponential-phase cells grown in TSB medium with 2 mM cystine were treated for 1 h with 20 mM H 2 O 2 and plated on BHI. Results represent the mean values for survival with standard deviations and are representative of at least two independent experiments.

    Article Snippet: S. aureus was grown in brain heart infusion (BHI) (Oxoid) or tryptic soy broth/agar (TSB/TSA) (Difco) .

    Techniques: Mutagenesis

    ESR determination of hydroxyl radicals formed in cultures of S. aureus treated with hydrogen peroxide or antibiotics. Hydroxyl-radical signals were monitored in BHI (A) or TSB (B) medium (filled diamonds) or in cultures of S. aureus grown in the corresponding

    Journal: Applied and Environmental Microbiology

    Article Title: Catalase Expression Is Modulated by Vancomycin and Ciprofloxacin and Influences the Formation of Free Radicals in Staphylococcus aureus Cultures

    doi: 10.1128/AEM.01199-15

    Figure Lengend Snippet: ESR determination of hydroxyl radicals formed in cultures of S. aureus treated with hydrogen peroxide or antibiotics. Hydroxyl-radical signals were monitored in BHI (A) or TSB (B) medium (filled diamonds) or in cultures of S. aureus grown in the corresponding

    Article Snippet: Staphylococcus aureus Newman (NTCT 8178) was cultivated in 25 ml of tryptic soy broth (TSB) or brain heart infusion broth (BHI) (Oxoid, Denmark) in a 250-ml narrow-neck Erlenmeyer flask at 37°C and 200 rpm.

    Techniques: Electron Paramagnetic Resonance

    Effect of untreated supernatant on Streptococcus mutans ( A ) Effect of untreated supernatant on Streptococcus mutans adherence. ( B ) Effect of untreated supernatant on Streptococcus mutans preformed biofilm Optical density (OD 545 nm) of Streptococcus mutans biofilm in the presence of untreated Lactobacillus sp. supernatants ( L. casei , L. reuteri , L. plantarum and L. salivarius ). Control: Streptococcus mutans grown in BHI broth. Data are expressed as the mean ± S.D. ** P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries‐inducing Streptococcus mutans

    doi: 10.1111/jcmm.13496

    Figure Lengend Snippet: Effect of untreated supernatant on Streptococcus mutans ( A ) Effect of untreated supernatant on Streptococcus mutans adherence. ( B ) Effect of untreated supernatant on Streptococcus mutans preformed biofilm Optical density (OD 545 nm) of Streptococcus mutans biofilm in the presence of untreated Lactobacillus sp. supernatants ( L. casei , L. reuteri , L. plantarum and L. salivarius ). Control: Streptococcus mutans grown in BHI broth. Data are expressed as the mean ± S.D. ** P

    Article Snippet: Lactobacillus sp. and Streptococcus mutans were cultured in deMan, Rogosa and Sharpe (MRS) and brain–heart infusion (BHI) media (Oxoid, Hampshire, Thermo Fisher Scientific, UK), respectively, at 37°C under anaerobic conditions using Oxoid Anaerogen® sachets (Thermo Fisher Scientific, UK).

    Techniques:

    Streptococcus mutans growth in the presence of treated and untreated Lactobacillus sp. supernatant. Optical density (OD) of Streptococcus mutans growth in the presence of treated and untreated Lactobacillus sp. supernatants ( L. casei , L. reuteri , L. plantarum and L. salivarius ). Control: Streptococcus mutans grown in BHI broth. Untreated: Spent Culture Supernatant (SCS) of each strain supernatant, pH treated: supernatant with adjusted pH 6.5, catalase treated: supernatant after addition of 0.5 mg/ml catalase enzyme and trypsin treated: supernatant after addition of 1 mg/ml trypsin enzyme. Data are expressed as the mean ± S.D., ** P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries‐inducing Streptococcus mutans

    doi: 10.1111/jcmm.13496

    Figure Lengend Snippet: Streptococcus mutans growth in the presence of treated and untreated Lactobacillus sp. supernatant. Optical density (OD) of Streptococcus mutans growth in the presence of treated and untreated Lactobacillus sp. supernatants ( L. casei , L. reuteri , L. plantarum and L. salivarius ). Control: Streptococcus mutans grown in BHI broth. Untreated: Spent Culture Supernatant (SCS) of each strain supernatant, pH treated: supernatant with adjusted pH 6.5, catalase treated: supernatant after addition of 0.5 mg/ml catalase enzyme and trypsin treated: supernatant after addition of 1 mg/ml trypsin enzyme. Data are expressed as the mean ± S.D., ** P

    Article Snippet: Lactobacillus sp. and Streptococcus mutans were cultured in deMan, Rogosa and Sharpe (MRS) and brain–heart infusion (BHI) media (Oxoid, Hampshire, Thermo Fisher Scientific, UK), respectively, at 37°C under anaerobic conditions using Oxoid Anaerogen® sachets (Thermo Fisher Scientific, UK).

    Techniques:

    Streptococcus mutans growth in the presence of untreated Lactobacillus sp. supernatant. Optical density (OD) of Streptococcus mutans growth in the presence of untreated Lactobacillus sp. supernatants ( L. casei , L. reuteri , L. plantarum and L. salivarius ). Control: Streptococcus mutans growth in BHI broth. Untreated: spent culture supernatant (SCS). Data are expressed as the mean ± S.D., *** P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries‐inducing Streptococcus mutans

    doi: 10.1111/jcmm.13496

    Figure Lengend Snippet: Streptococcus mutans growth in the presence of untreated Lactobacillus sp. supernatant. Optical density (OD) of Streptococcus mutans growth in the presence of untreated Lactobacillus sp. supernatants ( L. casei , L. reuteri , L. plantarum and L. salivarius ). Control: Streptococcus mutans growth in BHI broth. Untreated: spent culture supernatant (SCS). Data are expressed as the mean ± S.D., *** P

    Article Snippet: Lactobacillus sp. and Streptococcus mutans were cultured in deMan, Rogosa and Sharpe (MRS) and brain–heart infusion (BHI) media (Oxoid, Hampshire, Thermo Fisher Scientific, UK), respectively, at 37°C under anaerobic conditions using Oxoid Anaerogen® sachets (Thermo Fisher Scientific, UK).

    Techniques:

    Deletion of ArgR enhances survival of L. monocytogenes in the lethal acidic conditions. Overnight-grown L. monocytogenes wild-type and mutant strains were harvested, washed and then incubated in BHI broth (pre-adjusted to pH 3.5 using 3 M lactic acid, LA) at 37°C. Survivors were enumerated at regular intervals by plating serial dilutions on BHI plate. Data are expressed as Mean ± SD of recovery rate for each strain. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: Listeria monocytogenes 10403S Arginine Repressor ArgR Finely Tunes Arginine Metabolism Regulation under Acidic Conditions

    doi: 10.3389/fmicb.2017.00145

    Figure Lengend Snippet: Deletion of ArgR enhances survival of L. monocytogenes in the lethal acidic conditions. Overnight-grown L. monocytogenes wild-type and mutant strains were harvested, washed and then incubated in BHI broth (pre-adjusted to pH 3.5 using 3 M lactic acid, LA) at 37°C. Survivors were enumerated at regular intervals by plating serial dilutions on BHI plate. Data are expressed as Mean ± SD of recovery rate for each strain. ∗ P

    Article Snippet: L. monocytogenes was cultured in brain heart infusion (BHI) medium (Oxoid, Hampshire, England).

    Techniques: Mutagenesis, Incubation

    Growth of wild-type L. monocytogenes EGD ( filled circle ), Δ axyR mutant ( filled diagonal ), Δ phoP mutant ( filled square ) and Δ fri mutant ( filled triangle ) in BHI broth pH 5 adjusted with HCl ( a ) and in BHI broth supplemented with 0.5 M KCl ( b ). Overnight cultures were inoculated (1:100) into BHI broth supplemented with appropriate stressor, and cultures were incubated with shaking at 37 °C. Cell growth was measured spectrophotometrically by determining the optical density at 600 nm. Error bars , standard deviations from three independent experiments

    Journal: Archives of Microbiology

    Article Title: An essential role of a ferritin-like protein in acid stress tolerance of Listeria monocytogenes

    doi: 10.1007/s00203-014-1053-4

    Figure Lengend Snippet: Growth of wild-type L. monocytogenes EGD ( filled circle ), Δ axyR mutant ( filled diagonal ), Δ phoP mutant ( filled square ) and Δ fri mutant ( filled triangle ) in BHI broth pH 5 adjusted with HCl ( a ) and in BHI broth supplemented with 0.5 M KCl ( b ). Overnight cultures were inoculated (1:100) into BHI broth supplemented with appropriate stressor, and cultures were incubated with shaking at 37 °C. Cell growth was measured spectrophotometrically by determining the optical density at 600 nm. Error bars , standard deviations from three independent experiments

    Article Snippet: L. monocytogenes strains were grown in brain heart infusion (BHI) broth medium (Oxoid).

    Techniques: Mutagenesis, Incubation

    Transcriptional analysis of gene expression under temperature, acid and osmotic stress conditions using RT-PCR. Total RNA was isolated from exponential phase cultures of L. monocytogenes EGD strain grown in BHI at 37 °C ( 1 ) and exposed to 42 °C ( 2 ) or pH 5 ( 3 ) or 0.5 M KCl ( 4 ) for 90 min in each case. All RT-PCRs were performed three times from three separate RNA preparations. In all cases, control PCR were performed to ensure the complete removal of DNA from RNA preparations prior to reverse transcription

    Journal: Archives of Microbiology

    Article Title: An essential role of a ferritin-like protein in acid stress tolerance of Listeria monocytogenes

    doi: 10.1007/s00203-014-1053-4

    Figure Lengend Snippet: Transcriptional analysis of gene expression under temperature, acid and osmotic stress conditions using RT-PCR. Total RNA was isolated from exponential phase cultures of L. monocytogenes EGD strain grown in BHI at 37 °C ( 1 ) and exposed to 42 °C ( 2 ) or pH 5 ( 3 ) or 0.5 M KCl ( 4 ) for 90 min in each case. All RT-PCRs were performed three times from three separate RNA preparations. In all cases, control PCR were performed to ensure the complete removal of DNA from RNA preparations prior to reverse transcription

    Article Snippet: L. monocytogenes strains were grown in brain heart infusion (BHI) broth medium (Oxoid).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Polymerase Chain Reaction

    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in BHI with serum at 37°C and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)

    Journal: FEMS microbiology letters

    Article Title: Expression of the Collagen Adhesin ace by Enterococcus faecalis Strain OG1RF is not Repressed by Ers but Requires the Ers box

    doi: 10.1111/1574-6968.12146

    Figure Lengend Snippet: Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in BHI with serum at 37°C and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)

    Article Snippet: Bacteria were grown at 37°C in Luria-Bertani (LB) broth, Brain Heart Infusion (BHI) broth, M17 medium supplemented with 0.5% glucose (M17-G) or MM9YEG with 10 mM p -chloro-phenylalanine (p-Cl-Phe, Sigma-Aldrich Co.) ( ).

    Techniques: Expressing, Northern Blot

    FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Journal: Applied and Environmental Microbiology

    Article Title: A Single Mechanosensitive Channel Protects Francisella tularensis subsp. holarctica from Hypoosmotic Shock and Promotes Survival in the Aquatic Environment

    doi: 10.1128/AEM.02203-17

    Figure Lengend Snippet: FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Article Snippet: For downshock experiments conducted in medium, single colonies of each strain were picked from MH chocolate agar plates and grown in BHI broth supplemented with 300 mM NaCl (EMD Chemicals).

    Techniques:

    Expression of the fbsA gene (A and B) and adhesion to immobilized human fibrinogen (C and D). β-Galactosidase activity was measured in the WT-P fbsA , Δ rovS -P fbsA , and Δ shp -P fbsA strains for fbsA expression; each strain was grown in DMEMg (A) or BHI medium (B) at 37°C until it reached the early stationary phase (OD 600 , ~0.8). The adhesion assay was performed in microtiter wells previously coated with a fixed amount of human fibrinogen. A total of 10 6 CFU of culture grown in DMEMg (C) or BHI medium (D) was placed in each well, and the plates were then incubated for 30 min at 37°C. After the wells were washed to eliminate unbound bacteria, adhered cells were recovered and quantified in terms of CFU/ml. The data presented are the means ± SD of results from three independent experiments. Differences were considered to be highly significant if P values were

    Journal: mBio

    Article Title: RovS and Its Associated Signaling Peptide Form a Cell-To-Cell Communication System Required for Streptococcus agalactiae Pathogenesis

    doi: 10.1128/mBio.02306-14

    Figure Lengend Snippet: Expression of the fbsA gene (A and B) and adhesion to immobilized human fibrinogen (C and D). β-Galactosidase activity was measured in the WT-P fbsA , Δ rovS -P fbsA , and Δ shp -P fbsA strains for fbsA expression; each strain was grown in DMEMg (A) or BHI medium (B) at 37°C until it reached the early stationary phase (OD 600 , ~0.8). The adhesion assay was performed in microtiter wells previously coated with a fixed amount of human fibrinogen. A total of 10 6 CFU of culture grown in DMEMg (C) or BHI medium (D) was placed in each well, and the plates were then incubated for 30 min at 37°C. After the wells were washed to eliminate unbound bacteria, adhered cells were recovered and quantified in terms of CFU/ml. The data presented are the means ± SD of results from three independent experiments. Differences were considered to be highly significant if P values were

    Article Snippet: S. agalactiae strains were grown at 37°C in brain heart infusion (BHI) medium, Todd-Hewitt broth supplemented with yeast extract (THY), or Dulbecco’s modified Eagle’s medium (DMEM); the latter is a chemically defined medium (Sigma-Aldrich) with some modifications adapted to GBS growth as follows.

    Techniques: Expressing, Activity Assay, Cell Adhesion Assay, Incubation, IF-P

    The SHP/RovS cell-to-cell communication system is active in strain NEM316. (A) Effects of culture media on shp expression. β-Galactosidase activity was measured in the WT-P shp strain grown in DMEMg, THY, or BHI medium at 37°C until it reached the early stationary phase (OD 600 , ~0.8). (B) Expression kinetics of the shp and rovS genes. β-Galactosidase activity was measured during the growth of the WT-P shp (○) and WT-P rovS (□) strains. Dotted lines indicate the growth of each strain as measured at an OD 600 , and solid lines indicate β-galactosidase activity. (C) shp expression depends on RovS and SHP. β-Galactosidase activity was measured in the WT-P shp , Δ rovS -P shp , and Δ shp -P shp strains grown in DMEMg at 37°C until they reached the early stationary phase (OD 600 , ~0.8). The data presented are the means ± standard deviations (SD) of results from three independent experiments. Differences were considered to be extremely significant if P values were

    Journal: mBio

    Article Title: RovS and Its Associated Signaling Peptide Form a Cell-To-Cell Communication System Required for Streptococcus agalactiae Pathogenesis

    doi: 10.1128/mBio.02306-14

    Figure Lengend Snippet: The SHP/RovS cell-to-cell communication system is active in strain NEM316. (A) Effects of culture media on shp expression. β-Galactosidase activity was measured in the WT-P shp strain grown in DMEMg, THY, or BHI medium at 37°C until it reached the early stationary phase (OD 600 , ~0.8). (B) Expression kinetics of the shp and rovS genes. β-Galactosidase activity was measured during the growth of the WT-P shp (○) and WT-P rovS (□) strains. Dotted lines indicate the growth of each strain as measured at an OD 600 , and solid lines indicate β-galactosidase activity. (C) shp expression depends on RovS and SHP. β-Galactosidase activity was measured in the WT-P shp , Δ rovS -P shp , and Δ shp -P shp strains grown in DMEMg at 37°C until they reached the early stationary phase (OD 600 , ~0.8). The data presented are the means ± standard deviations (SD) of results from three independent experiments. Differences were considered to be extremely significant if P values were

    Article Snippet: S. agalactiae strains were grown at 37°C in brain heart infusion (BHI) medium, Todd-Hewitt broth supplemented with yeast extract (THY), or Dulbecco’s modified Eagle’s medium (DMEM); the latter is a chemically defined medium (Sigma-Aldrich) with some modifications adapted to GBS growth as follows.

    Techniques: Expressing, Activity Assay, IF-P

    Protease activation in the extracellular protein fraction from P. gingivalis FLL93. P. gingivalis was grown to stationary phase (OD 600 of 1.3) in 1 liter of BHI broth supplemented with hemin and vitamin K. Acetone (37.5%)-precipitated proteins were mixed with 8 M urea and were incubated at 4°C for 1 h. The urea was slowly removed from the mixture by centrifugation (10,000 × g ) in a Millipore filtration unit with the addition of increasing volumes of 100 mM Tris-HCl buffer (pH 7.4). Activities for Rgp and Kgp were tested. Error bars indicate the standard errors of the means of three independent trials.

    Journal: Infection and Immunity

    Article Title: The vimE Gene Downstream of vimA Is Independently Expressed and Is Involved in Modulating Proteolytic Activity in Porphyromonas gingivalis W83

    doi: 10.1128/IAI.72.10.5555-5564.2004

    Figure Lengend Snippet: Protease activation in the extracellular protein fraction from P. gingivalis FLL93. P. gingivalis was grown to stationary phase (OD 600 of 1.3) in 1 liter of BHI broth supplemented with hemin and vitamin K. Acetone (37.5%)-precipitated proteins were mixed with 8 M urea and were incubated at 4°C for 1 h. The urea was slowly removed from the mixture by centrifugation (10,000 × g ) in a Millipore filtration unit with the addition of increasing volumes of 100 mM Tris-HCl buffer (pH 7.4). Activities for Rgp and Kgp were tested. Error bars indicate the standard errors of the means of three independent trials.

    Article Snippet: P. gingivalis strains were grown in brain heart infusion (BHI) broth supplemented with 0.5% yeast extract (both from Difco Laboratories, Detroit, Mich.), hemin (5 μg/ml), vitamin K (0.5 μg/ml), and cysteine (0.1%) (all from Sigma-Aldrich, St. Louis, Mo.).

    Techniques: Activation Assay, Incubation, Centrifugation, Filtration

    Inactivation of vimE resulted in decreased proteolytic activity of P. gingivalis. P. gingivalis was grown to late log phase (OD 600 of 0.7 to 0.8) or stationary phase (OD 600 of 1.4 to 1.5) in BHI broth supplemented with yeast extract, hemin, and vitamin K. Activities against Rgp (A) and Kgp (B) were tested in whole-cell culture. The results shown are representative of three independent experiments in triplicate. E, exponential phase; L, stationary phase. Error bars indicate the standard errors of the means of three independent trials.

    Journal: Infection and Immunity

    Article Title: The vimE Gene Downstream of vimA Is Independently Expressed and Is Involved in Modulating Proteolytic Activity in Porphyromonas gingivalis W83

    doi: 10.1128/IAI.72.10.5555-5564.2004

    Figure Lengend Snippet: Inactivation of vimE resulted in decreased proteolytic activity of P. gingivalis. P. gingivalis was grown to late log phase (OD 600 of 0.7 to 0.8) or stationary phase (OD 600 of 1.4 to 1.5) in BHI broth supplemented with yeast extract, hemin, and vitamin K. Activities against Rgp (A) and Kgp (B) were tested in whole-cell culture. The results shown are representative of three independent experiments in triplicate. E, exponential phase; L, stationary phase. Error bars indicate the standard errors of the means of three independent trials.

    Article Snippet: P. gingivalis strains were grown in brain heart infusion (BHI) broth supplemented with 0.5% yeast extract (both from Difco Laboratories, Detroit, Mich.), hemin (5 μg/ml), vitamin K (0.5 μg/ml), and cysteine (0.1%) (all from Sigma-Aldrich, St. Louis, Mo.).

    Techniques: Activity Assay, Cell Culture

    Immunoreactivity of the RgpB proenzyme in P. gingivalis FLL93. Western blot analysis using specific RgpB proenzyme antibodies as probes was done on the extracellular fractions from P. gingivalis W83 (lane 1), FLL92 (lane 2), and FLL93 (lane 3). All lanes contained 20 μg of acetone (37.5%)-precipitated proteins from the supernatant fractions of cultures in BHI medium at exponential growth phase.

    Journal: Infection and Immunity

    Article Title: The vimE Gene Downstream of vimA Is Independently Expressed and Is Involved in Modulating Proteolytic Activity in Porphyromonas gingivalis W83

    doi: 10.1128/IAI.72.10.5555-5564.2004

    Figure Lengend Snippet: Immunoreactivity of the RgpB proenzyme in P. gingivalis FLL93. Western blot analysis using specific RgpB proenzyme antibodies as probes was done on the extracellular fractions from P. gingivalis W83 (lane 1), FLL92 (lane 2), and FLL93 (lane 3). All lanes contained 20 μg of acetone (37.5%)-precipitated proteins from the supernatant fractions of cultures in BHI medium at exponential growth phase.

    Article Snippet: P. gingivalis strains were grown in brain heart infusion (BHI) broth supplemented with 0.5% yeast extract (both from Difco Laboratories, Detroit, Mich.), hemin (5 μg/ml), vitamin K (0.5 μg/ml), and cysteine (0.1%) (all from Sigma-Aldrich, St. Louis, Mo.).

    Techniques: Western Blot

    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. monocytogenes in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on BHI agar plate.

    Journal: PLoS ONE

    Article Title: Protective Role of Heme Oxygenase-1 in Listeria monocytogenes-Induced Abortion

    doi: 10.1371/journal.pone.0025046

    Figure Lengend Snippet: Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. monocytogenes in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on BHI agar plate.

    Article Snippet: Bacterial strains L. monocytogenes EGD was maintained as a frozen glycerol stock and cultured in brain heart infusion (BHI) broth (Becton Dickinson) or on BHI broth containing 1.5% agar.

    Techniques: Expressing, Mouse Assay, Infection

    Decreased HO-1 and Bcl-XL expression in TG cells infected with L. monocytogenes . (A) TG cells were first treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 6 h, HO-1 and Bcl-XL expression was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) TG cells were treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 0.5, 2, and 6 h incubation, the infected cells were washed with PBS and lysed with cold distilled water. CFU were determined by serial dilution on BHI agar plates. (C) L. monocytogenes was deposited on TG cells by centrifugation at 150×g for 10 min at room temperature, incubated for 6 h, fixed, and stained. The figure shows FITC-labeled bacteria (green) and Alexa Fluor 594-labeled actin filaments (red) merged images. The left-hand panel shows untreated cells, the center panel Co-PP (9 µg/ml)-treated cells, and the right-hand panel, cytochalasin D-treated cells.

    Journal: PLoS ONE

    Article Title: Protective Role of Heme Oxygenase-1 in Listeria monocytogenes-Induced Abortion

    doi: 10.1371/journal.pone.0025046

    Figure Lengend Snippet: Decreased HO-1 and Bcl-XL expression in TG cells infected with L. monocytogenes . (A) TG cells were first treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 6 h, HO-1 and Bcl-XL expression was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) TG cells were treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 0.5, 2, and 6 h incubation, the infected cells were washed with PBS and lysed with cold distilled water. CFU were determined by serial dilution on BHI agar plates. (C) L. monocytogenes was deposited on TG cells by centrifugation at 150×g for 10 min at room temperature, incubated for 6 h, fixed, and stained. The figure shows FITC-labeled bacteria (green) and Alexa Fluor 594-labeled actin filaments (red) merged images. The left-hand panel shows untreated cells, the center panel Co-PP (9 µg/ml)-treated cells, and the right-hand panel, cytochalasin D-treated cells.

    Article Snippet: Bacterial strains L. monocytogenes EGD was maintained as a frozen glycerol stock and cultured in brain heart infusion (BHI) broth (Becton Dickinson) or on BHI broth containing 1.5% agar.

    Techniques: Expressing, Infection, Cell Culture, Incubation, Serial Dilution, Centrifugation, Staining, Labeling

    Prevention of cell death by HO-1 and Bcl-XL expression. (A) TG cells were treated for 48 h with either siRNA targeting HO-1, Bcl-XL, or control siRNA (QIAGEN AllStars Negative Control). Bcl-XL overexpression was achieved by transfecting the cells with pcDNA4/TO-Bcl-XL. HO-1 and Bcl-XL expression was monitored by immunoblotting. β-actin was used as an internal control. A representative immunoblot of three independent experiments is shown. (B) TG cells were infected with L. monocytogenes . The infected cells were cultured with media containing 50 µg/ml gentamicin for 2, 6, and 12 h. The cells were then washed with PBS and lysed with cold distilled water. CFU was determined by serial dilution on BHI agar plates. All values represent the average and the standard deviation of three identical experiments. (C) Cell death was determined using the JC-1 Mitochondrial Membrane Potential Assay Kit. One hundred TG cells per coverslip were examined to determine the total number of live or dead cells. All values represent the average and the standard deviation of three identical experiments. Statistically significant differences compared with the control are indicated by asterisks (*, P

    Journal: PLoS ONE

    Article Title: Protective Role of Heme Oxygenase-1 in Listeria monocytogenes-Induced Abortion

    doi: 10.1371/journal.pone.0025046

    Figure Lengend Snippet: Prevention of cell death by HO-1 and Bcl-XL expression. (A) TG cells were treated for 48 h with either siRNA targeting HO-1, Bcl-XL, or control siRNA (QIAGEN AllStars Negative Control). Bcl-XL overexpression was achieved by transfecting the cells with pcDNA4/TO-Bcl-XL. HO-1 and Bcl-XL expression was monitored by immunoblotting. β-actin was used as an internal control. A representative immunoblot of three independent experiments is shown. (B) TG cells were infected with L. monocytogenes . The infected cells were cultured with media containing 50 µg/ml gentamicin for 2, 6, and 12 h. The cells were then washed with PBS and lysed with cold distilled water. CFU was determined by serial dilution on BHI agar plates. All values represent the average and the standard deviation of three identical experiments. (C) Cell death was determined using the JC-1 Mitochondrial Membrane Potential Assay Kit. One hundred TG cells per coverslip were examined to determine the total number of live or dead cells. All values represent the average and the standard deviation of three identical experiments. Statistically significant differences compared with the control are indicated by asterisks (*, P

    Article Snippet: Bacterial strains L. monocytogenes EGD was maintained as a frozen glycerol stock and cultured in brain heart infusion (BHI) broth (Becton Dickinson) or on BHI broth containing 1.5% agar.

    Techniques: Expressing, Negative Control, Over Expression, Infection, Cell Culture, Serial Dilution, Standard Deviation

    Growth-dependent bioluminescence in phage-infected B. anthracis cells (A) Overnight Sterne cultures were diluted (1:100) with BHI broth and incubated at 35°C with continuous shaking (250 rpm) and assessed at various times for A 600 . At the times indicated (denoted by the arrows), samples were harvested and mixed with 10 9 PFU mL −1 (final concentration) phage and bioluminescence was determined after 1 h (B) . Numbers indicate the mean (n=3) ± SD. *p

    Journal: Journal of applied microbiology

    Article Title: Detection of Bacillus anthracis Spores from Environmental Water Using Bioluminescent Reporter Phage

    doi: 10.1111/jam.13569

    Figure Lengend Snippet: Growth-dependent bioluminescence in phage-infected B. anthracis cells (A) Overnight Sterne cultures were diluted (1:100) with BHI broth and incubated at 35°C with continuous shaking (250 rpm) and assessed at various times for A 600 . At the times indicated (denoted by the arrows), samples were harvested and mixed with 10 9 PFU mL −1 (final concentration) phage and bioluminescence was determined after 1 h (B) . Numbers indicate the mean (n=3) ± SD. *p

    Article Snippet: All strains ( B. anthracis and B. cereus ) were grown in BD™ Brain Heart Infusion broth (BHI), soft agar (broth and 0.7% Bacto agar) or broth plus 1.5% agar.

    Techniques: Infection, Incubation, Concentration Assay

    Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, Luria-Bertani; BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.

    Journal: PLoS ONE

    Article Title: A novel bacteriocin from Enterococcus faecalis 478 exhibits a potent activity against vancomycin-resistant enterococci

    doi: 10.1371/journal.pone.0186415

    Figure Lengend Snippet: Optimization of bacteriocin-production by E . faecalis EF478. (A) Culture media (B) Medium pH (C) Incubation temperature (D) Incubation time. LB, Luria-Bertani; BHI, brain heart infusion; MRS, De Man Rogosa Sharpe. AU/ml: arbitrary unit.

    Article Snippet: Three growth media namely Luria-Bertani medium (LB), De Man Rogosa Sharpe medium (MRS) and brain heart infusion broth (BHI) (all were purchased from Merck, Darmstadt, Germany) were used for comparison.

    Techniques: Incubation

    Correlation of bioluminescence (relative light units [RLU]) and culture regimen-dependent viable cell count (CFU per square centimeter) measured at 10°C. The A lines show the correlation on cheese after 14 days of storage (open squares) and during the first 3 days after inoculation (closed squares). The B lines show the correlation for Y . enterocolitica on BHI agar with 2.5% salt (closed triangles) and without salt (open triangles) on the first 5 days. The background luminescence of cheese and BHI agar has been subtracted. Immediately after the light intensity was measured, the plates or cheeses were used to determine the viable counts (see text).

    Journal: Applied and Environmental Microbiology

    Article Title: Sensitive In Situ Monitoring of a Recombinant Bioluminescent Yersinia enterocolitica Reporter Mutant in Real Time on Camembert Cheese

    doi: 10.1128/AEM.68.11.5737-5740.2002

    Figure Lengend Snippet: Correlation of bioluminescence (relative light units [RLU]) and culture regimen-dependent viable cell count (CFU per square centimeter) measured at 10°C. The A lines show the correlation on cheese after 14 days of storage (open squares) and during the first 3 days after inoculation (closed squares). The B lines show the correlation for Y . enterocolitica on BHI agar with 2.5% salt (closed triangles) and without salt (open triangles) on the first 5 days. The background luminescence of cheese and BHI agar has been subtracted. Immediately after the light intensity was measured, the plates or cheeses were used to determine the viable counts (see text).

    Article Snippet: They were grown in brain heart infusion (BHI) broth (Merck) supplemented with 2.5% NaCl (10°C, 48 h).

    Techniques: Cell Counting

    Influence of growth temperature and growth phase on bioluminescence of Y . enterocolitica B94. The mutant was grown in BHI broth with 2.5% salt at different temperatures to an OD 600 of 0.15 (5 × 10 7 CFU/ml) (triangles) and to an OD 600 of 1.1 (4.5 × 10 8 CFU/ml) (diamonds). A 100-μl sample of the grown culture was spread on BHI plates preadjusted to 10°C. After 5 min at 10°C, bioluminescence was measured at 10°C. RLU, relative light units; 1.0E+02, 1 × 10 2 .

    Journal: Applied and Environmental Microbiology

    Article Title: Sensitive In Situ Monitoring of a Recombinant Bioluminescent Yersinia enterocolitica Reporter Mutant in Real Time on Camembert Cheese

    doi: 10.1128/AEM.68.11.5737-5740.2002

    Figure Lengend Snippet: Influence of growth temperature and growth phase on bioluminescence of Y . enterocolitica B94. The mutant was grown in BHI broth with 2.5% salt at different temperatures to an OD 600 of 0.15 (5 × 10 7 CFU/ml) (triangles) and to an OD 600 of 1.1 (4.5 × 10 8 CFU/ml) (diamonds). A 100-μl sample of the grown culture was spread on BHI plates preadjusted to 10°C. After 5 min at 10°C, bioluminescence was measured at 10°C. RLU, relative light units; 1.0E+02, 1 × 10 2 .

    Article Snippet: They were grown in brain heart infusion (BHI) broth (Merck) supplemented with 2.5% NaCl (10°C, 48 h).

    Techniques: Mutagenesis

    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. monocytogenes EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in BHI from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl

    Journal: Bioengineered Bugs

    Article Title: Development and optimization of an EGFP-based reporter for measuring the general stress response in Listeria monocytogenes

    doi: 10.4161/bbug.19476

    Figure Lengend Snippet: Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. monocytogenes EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in BHI from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl

    Article Snippet: L. monocytogenes strains were grown in Brain Heart Infusion (BHI) broth or agar (LabM) at 37°C unless otherwise stated.

    Techniques: Expressing

    P. aeruginosa supports the growth of F. nucleatum in both planktonic and biofilm settings under aerobic conditions. Tubes containing 5 mL of BHI broth and a cellulose disk were inoculated with ~10 6 CFU of P. aeruginosa and 10 2 CFU of F. nucleatum and incubated aerobically at 37°C for time indicated on graph. (a) The disks were removed and rinsed to remove planktonic bacteria, and the numbers of CFU in the biofilm (CFU/disk) were determined. (b) The numbers of planktonic bacteria (CFU/mL) were also determined. Values in (a) and (b) represent the means of three independent experiments ± SEM; ∗ P

    Journal: International Journal of Otolaryngology

    Article Title: Planktonic Growth of Pseudomonas aeruginosa around a Dual-Species Biofilm Supports the Growth of Fusobacterium nucleatum within That Biofilm

    doi: 10.1155/2017/3037191

    Figure Lengend Snippet: P. aeruginosa supports the growth of F. nucleatum in both planktonic and biofilm settings under aerobic conditions. Tubes containing 5 mL of BHI broth and a cellulose disk were inoculated with ~10 6 CFU of P. aeruginosa and 10 2 CFU of F. nucleatum and incubated aerobically at 37°C for time indicated on graph. (a) The disks were removed and rinsed to remove planktonic bacteria, and the numbers of CFU in the biofilm (CFU/disk) were determined. (b) The numbers of planktonic bacteria (CFU/mL) were also determined. Values in (a) and (b) represent the means of three independent experiments ± SEM; ∗ P

    Article Snippet: For general growth, the strains were grown aerobically (18.6% ambient oxygen) or anaerobically (0% oxygen) at 37°C for up to 96 h in 37°C in brain heart infusion (BHI) broth or on BHI agar plates (CRITERION; Hardy Diagnostics, Santa Maria, CA).

    Techniques: Incubation

    The growth of P. aeruginosa significantly reduces the level of dissolved oxygen in broth. Fifty mL conical tubes containing 15 mL of BHI broth were inoculated with ~10 6 CFU of PAO1, 10 2 CFU of VPI 4355, or both organisms and incubated aerobically at 37°C. The level of DO 2 (mg/L) was measured at 24 h after inoculation. As a control, the level of DO 2 in uninoculated BHI broth was measured at time 0 for a baseline and at 24 h. Values represent the means of three independent experiments ± SEM; ∗∗∗∗ P

    Journal: International Journal of Otolaryngology

    Article Title: Planktonic Growth of Pseudomonas aeruginosa around a Dual-Species Biofilm Supports the Growth of Fusobacterium nucleatum within That Biofilm

    doi: 10.1155/2017/3037191

    Figure Lengend Snippet: The growth of P. aeruginosa significantly reduces the level of dissolved oxygen in broth. Fifty mL conical tubes containing 15 mL of BHI broth were inoculated with ~10 6 CFU of PAO1, 10 2 CFU of VPI 4355, or both organisms and incubated aerobically at 37°C. The level of DO 2 (mg/L) was measured at 24 h after inoculation. As a control, the level of DO 2 in uninoculated BHI broth was measured at time 0 for a baseline and at 24 h. Values represent the means of three independent experiments ± SEM; ∗∗∗∗ P

    Article Snippet: For general growth, the strains were grown aerobically (18.6% ambient oxygen) or anaerobically (0% oxygen) at 37°C for up to 96 h in 37°C in brain heart infusion (BHI) broth or on BHI agar plates (CRITERION; Hardy Diagnostics, Santa Maria, CA).

    Techniques: Incubation

    Growth of F. nucleatum within the P. aeruginosa/F. nucleatum biofilm depends on the environment around the biofilm. PAO1 and VPI 4355 were inoculated into two sets of tubes of BHI broth containing cellulose disks as described in Figure 2 and incubated aerobically for 24 h at 37°C. (a) Disks were removed from one set of tubes, placed on the surface of BHI agar plates, and incubated aerobically for 48 h at 37°C. At intervals indicated on the graph, the disks were removed from the plates and the number of CFU/disk was determined. (b) Disks from the second set of tubes were transferred to fresh tubes of BHI broth and incubated aerobically for 48 h at 37°C. At intervals indicated on the graph, the disks were removed from the broth and the number of CFU/disk was determined. Values in (a) and (b) represent the means of three independent experiments ± SEM; ∗ P

    Journal: International Journal of Otolaryngology

    Article Title: Planktonic Growth of Pseudomonas aeruginosa around a Dual-Species Biofilm Supports the Growth of Fusobacterium nucleatum within That Biofilm

    doi: 10.1155/2017/3037191

    Figure Lengend Snippet: Growth of F. nucleatum within the P. aeruginosa/F. nucleatum biofilm depends on the environment around the biofilm. PAO1 and VPI 4355 were inoculated into two sets of tubes of BHI broth containing cellulose disks as described in Figure 2 and incubated aerobically for 24 h at 37°C. (a) Disks were removed from one set of tubes, placed on the surface of BHI agar plates, and incubated aerobically for 48 h at 37°C. At intervals indicated on the graph, the disks were removed from the plates and the number of CFU/disk was determined. (b) Disks from the second set of tubes were transferred to fresh tubes of BHI broth and incubated aerobically for 48 h at 37°C. At intervals indicated on the graph, the disks were removed from the broth and the number of CFU/disk was determined. Values in (a) and (b) represent the means of three independent experiments ± SEM; ∗ P

    Article Snippet: For general growth, the strains were grown aerobically (18.6% ambient oxygen) or anaerobically (0% oxygen) at 37°C for up to 96 h in 37°C in brain heart infusion (BHI) broth or on BHI agar plates (CRITERION; Hardy Diagnostics, Santa Maria, CA).

    Techniques: Incubation

    P. aeruginosa supports the growth of F. nucleatum under aerobic conditions. P. aeruginosa strain PAO1 and F. nucleatum strain VPI 4355 were inoculated into BHI broth individually or in coculture and incubated at 37°C under aerobic or anaerobic conditions. The number of CFU/mL was determined. Values represent the means of three independent experiments ± SEM.

    Journal: International Journal of Otolaryngology

    Article Title: Planktonic Growth of Pseudomonas aeruginosa around a Dual-Species Biofilm Supports the Growth of Fusobacterium nucleatum within That Biofilm

    doi: 10.1155/2017/3037191

    Figure Lengend Snippet: P. aeruginosa supports the growth of F. nucleatum under aerobic conditions. P. aeruginosa strain PAO1 and F. nucleatum strain VPI 4355 were inoculated into BHI broth individually or in coculture and incubated at 37°C under aerobic or anaerobic conditions. The number of CFU/mL was determined. Values represent the means of three independent experiments ± SEM.

    Article Snippet: For general growth, the strains were grown aerobically (18.6% ambient oxygen) or anaerobically (0% oxygen) at 37°C for up to 96 h in 37°C in brain heart infusion (BHI) broth or on BHI agar plates (CRITERION; Hardy Diagnostics, Santa Maria, CA).

    Techniques: Incubation

    Growth of P. aeruginosa creates anaerobic conditions within 4 h of inoculation. Fifty mL tubes of BHI broth were left uninoculated or inoculated with 10 6 CFU PAO1 and incubated for 24 h at 37°C. Levels of DO 2 were measured at times indicated. Values represent the means of three independent experiments ± SEM; ∗∗∗∗ P

    Journal: International Journal of Otolaryngology

    Article Title: Planktonic Growth of Pseudomonas aeruginosa around a Dual-Species Biofilm Supports the Growth of Fusobacterium nucleatum within That Biofilm

    doi: 10.1155/2017/3037191

    Figure Lengend Snippet: Growth of P. aeruginosa creates anaerobic conditions within 4 h of inoculation. Fifty mL tubes of BHI broth were left uninoculated or inoculated with 10 6 CFU PAO1 and incubated for 24 h at 37°C. Levels of DO 2 were measured at times indicated. Values represent the means of three independent experiments ± SEM; ∗∗∗∗ P

    Article Snippet: For general growth, the strains were grown aerobically (18.6% ambient oxygen) or anaerobically (0% oxygen) at 37°C for up to 96 h in 37°C in brain heart infusion (BHI) broth or on BHI agar plates (CRITERION; Hardy Diagnostics, Santa Maria, CA).

    Techniques: Incubation

    Invasion of CPEK monolayers by S. pseudintermedius strains. S. pseudintermedius cells were incubated with CPEK monolayers. Extracellular bacteria were killed with gentamicin, and internalized bacteria were quantified by plating lysates on BHI agar. The assay was performed three times. Each point represents the average value for three replicas, and error bars show the standard deviations (SD). Statistically significant ( P

    Journal: Infection and Immunity

    Article Title: Fibronectin Binding Proteins SpsD and SpsL Both Support Invasion of Canine Epithelial Cells by Staphylococcus pseudintermedius

    doi: 10.1128/IAI.00542-15

    Figure Lengend Snippet: Invasion of CPEK monolayers by S. pseudintermedius strains. S. pseudintermedius cells were incubated with CPEK monolayers. Extracellular bacteria were killed with gentamicin, and internalized bacteria were quantified by plating lysates on BHI agar. The assay was performed three times. Each point represents the average value for three replicas, and error bars show the standard deviations (SD). Statistically significant ( P

    Article Snippet: S. pseudintermedius ED99 and its mutants were grown in brain heart infusion (BHI) broth (VWR International Srl, Milan, Italy) at 37°C with shaking.

    Techniques: Incubation

    Production of cytolysins by L. monocytogenes mutants. Wild-type and mutant L. monocytogenes strains were cultured first in BHI medium and then transferred into RPMI 1640 medium. Three hours after cultivation, the supernatant was collected by centrifugation. (A) An aliquot of supernatant was applied to SDS-polyacrylamide gels and transferred onto a polyvinylidene difluoride membrane. Immunoblotting was performed with anti-LLO polyclonal antibody (left) or anti-ILO polyclonal antibody (right). (B) Culture supernatants were twofold serially diluted with PBS and mixed with an equal volume of a 2% suspension of SRBCs. After incubation for 30 min at 37°C, the hemolytic activity was measured by the amount of hemoglobin released into the supernatant. One hundred percent hemolysis was defined as the level of hemoglobin released after treatment with 2% Triton X-100.

    Journal: Infection and Immunity

    Article Title: Cytolysin-Dependent Escape of the Bacterium from the Phagosome Is Required but Not Sufficient for Induction of the Th1 Immune Response against Listeria monocytogenes Infection: Distinct Role of Listeriolysin O Determined by Cytolysin Gene Replacement ▿

    doi: 10.1128/IAI.01779-06

    Figure Lengend Snippet: Production of cytolysins by L. monocytogenes mutants. Wild-type and mutant L. monocytogenes strains were cultured first in BHI medium and then transferred into RPMI 1640 medium. Three hours after cultivation, the supernatant was collected by centrifugation. (A) An aliquot of supernatant was applied to SDS-polyacrylamide gels and transferred onto a polyvinylidene difluoride membrane. Immunoblotting was performed with anti-LLO polyclonal antibody (left) or anti-ILO polyclonal antibody (right). (B) Culture supernatants were twofold serially diluted with PBS and mixed with an equal volume of a 2% suspension of SRBCs. After incubation for 30 min at 37°C, the hemolytic activity was measured by the amount of hemoglobin released into the supernatant. One hundred percent hemolysis was defined as the level of hemoglobin released after treatment with 2% Triton X-100.

    Article Snippet: Bacteria were grown overnight in brain heart infusion (BHI) broth (EIKEN CHEMICAL, Tokyo, Japan) at 37°C with shaking.

    Techniques: Mutagenesis, Cell Culture, Centrifugation, Incubation, Activity Assay