brain heart infusion bhi broth Search Results


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  • 99
    Thermo Fisher brain heart infusion bhi broth
    Detection of E. faecalis EbpC on the cell surface by immunoelectron microscopy. Anti-EbpC MAb-probed <t>OG1RF</t> cells grown in <t>BHI</t> were immunogold labeled, negatively stained with 1% uranyl acetate, and viewed by transmission electron microscopy.
    Brain Heart Infusion Bhi Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore bhi broth
    FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in <t>BHI</t> broth. Bacteria were grown in BHI broth plus 300 mM <t>NaCl,</t> and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P
    Bhi Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco brain heart infusion bhi broth
    Protease activation in the extracellular protein fraction from P. <t>gingivalis</t> FLL93. P. gingivalis was grown to stationary phase (OD 600 of 1.3) in 1 liter of <t>BHI</t> broth supplemented with hemin and vitamin K. Acetone (37.5%)-precipitated proteins were mixed with 8 M urea and were incubated at 4°C for 1 h. The urea was slowly removed from the mixture by centrifugation (10,000 × g ) in a Millipore filtration unit with the addition of increasing volumes of 100 mM Tris-HCl buffer (pH 7.4). Activities for Rgp and Kgp were tested. Error bars indicate the standard errors of the means of three independent trials.
    Brain Heart Infusion Bhi Broth, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 1650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson brain heart infusion bhi broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Bhi Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 1043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher brain heart infusion broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Broth, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco brain heart infusion broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Broth, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 2271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson brain heart infusion broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 1491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA brain heart infusion bhi broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Bhi Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories brain heart infusion bhi broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Bhi Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA brain heart infusion broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Broth, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories brain heart infusion broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Broth, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 349 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co brain heart infusion broth
    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. <t>monocytogenes</t> in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on <t>BHI</t> agar plate.
    Brain Heart Infusion Broth, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lab M Ltd brain heart infusion bhi broth
    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. <t>monocytogenes</t> EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in <t>BHI</t> from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl
    Brain Heart Infusion Bhi Broth, supplied by Lab M Ltd, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co brain heart infusion bhi broth
    Correlation of bioluminescence (relative light units [RLU]) and culture regimen-dependent viable cell count (CFU per square centimeter) measured at <t>10°C.</t> The A lines show the correlation on cheese after 14 days of storage (open squares) and during the first 3 days after inoculation (closed squares). The B lines show the correlation for Y . enterocolitica on <t>BHI</t> agar with 2.5% salt (closed triangles) and without salt (open triangles) on the first 5 days. The background luminescence of cheese and BHI agar has been subtracted. Immediately after the light intensity was measured, the plates or cheeses were used to determine the viable counts (see text).
    Brain Heart Infusion Bhi Broth, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher remel brain heart infusion bhi broth media for automation mfa tubes
    Correlation of bioluminescence (relative light units [RLU]) and culture regimen-dependent viable cell count (CFU per square centimeter) measured at <t>10°C.</t> The A lines show the correlation on cheese after 14 days of storage (open squares) and during the first 3 days after inoculation (closed squares). The B lines show the correlation for Y . enterocolitica on <t>BHI</t> agar with 2.5% salt (closed triangles) and without salt (open triangles) on the first 5 days. The background luminescence of cheese and BHI agar has been subtracted. Immediately after the light intensity was measured, the plates or cheeses were used to determine the viable counts (see text).
    Remel Brain Heart Infusion Bhi Broth Media For Automation Mfa Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Detection of E. faecalis EbpC on the cell surface by immunoelectron microscopy. Anti-EbpC MAb-probed OG1RF cells grown in BHI were immunogold labeled, negatively stained with 1% uranyl acetate, and viewed by transmission electron microscopy.

    Journal: Infection and Immunity

    Article Title: Targeting Pili in Enterococcal Pathogenesis

    doi: 10.1128/IAI.01403-13

    Figure Lengend Snippet: Detection of E. faecalis EbpC on the cell surface by immunoelectron microscopy. Anti-EbpC MAb-probed OG1RF cells grown in BHI were immunogold labeled, negatively stained with 1% uranyl acetate, and viewed by transmission electron microscopy.

    Article Snippet: Briefly, overnight-cultured OG1RF was inoculated into 20 ml brain heart infusion (BHI) broth (Gibco) at a starting optical density at 600 nm (OD600 ) of 0.05 and harvested at mid-log phase.

    Techniques: Immuno-Electron Microscopy, Labeling, Staining, Transmission Assay, Electron Microscopy

    Oxacillin susceptibility and autolysis phenotypes of strains USA300 and HoR34. (A) Oxacillin MICs of strains USA300, HoR34, and HoR34 carrying plasmids pLI50 (control), p gdpP , p guaA , and p camS determined using Etests. (B) Autolytic activity in USA300 and HoR34. Strains USA300, HoR34, and HoR34 carrying plasmids pLI50 (control), p gdpP , p guaA , and p camS , and a USA300 JE2 atl mutant (negative control) were grown to early exponential phase in BHI at 37°C, washed in phosphate-buffered saline (PBS), and adjusted to an A 600 of 1.0 in 0.01% Triton X-100. The A 600 was measured initially and at 15-min intervals thereafter with shaking incubation at 37°C. Autolytic activity is expressed as a percentage of the initial A 600 . Average results from three independent experiments are shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Tandem Amplification of the Staphylococcal Cassette Chromosome mec Element Can Drive High-Level Methicillin Resistance in Methicillin-Resistant Staphylococcus aureus

    doi: 10.1128/AAC.00869-17

    Figure Lengend Snippet: Oxacillin susceptibility and autolysis phenotypes of strains USA300 and HoR34. (A) Oxacillin MICs of strains USA300, HoR34, and HoR34 carrying plasmids pLI50 (control), p gdpP , p guaA , and p camS determined using Etests. (B) Autolytic activity in USA300 and HoR34. Strains USA300, HoR34, and HoR34 carrying plasmids pLI50 (control), p gdpP , p guaA , and p camS , and a USA300 JE2 atl mutant (negative control) were grown to early exponential phase in BHI at 37°C, washed in phosphate-buffered saline (PBS), and adjusted to an A 600 of 1.0 in 0.01% Triton X-100. The A 600 was measured initially and at 15-min intervals thereafter with shaking incubation at 37°C. Autolytic activity is expressed as a percentage of the initial A 600 . Average results from three independent experiments are shown.

    Article Snippet: Bacterial strains used in this study are listed in and were grown at 37°C in LB (Sigma), brain heart infusion (BHI) (Oxoid), Mueller-Hinton (Oxoid), or nutrient (Oxoid) broth supplemented with ampicillin (50 μg/ml), oxacillin (0.5, 64, 100, or 130 μg/ml), chloramphenicol (10 μg/ml), or erythromycin (10 μg/ml) as indicated.

    Techniques: Activity Assay, Mutagenesis, Negative Control, Incubation

    ESR determination of hydroxyl radicals formed in cultures of S. aureus treated with hydrogen peroxide or antibiotics. Hydroxyl-radical signals were monitored in BHI (A) or TSB (B) medium (filled diamonds) or in cultures of S. aureus grown in the corresponding

    Journal: Applied and Environmental Microbiology

    Article Title: Catalase Expression Is Modulated by Vancomycin and Ciprofloxacin and Influences the Formation of Free Radicals in Staphylococcus aureus Cultures

    doi: 10.1128/AEM.01199-15

    Figure Lengend Snippet: ESR determination of hydroxyl radicals formed in cultures of S. aureus treated with hydrogen peroxide or antibiotics. Hydroxyl-radical signals were monitored in BHI (A) or TSB (B) medium (filled diamonds) or in cultures of S. aureus grown in the corresponding

    Article Snippet: Staphylococcus aureus Newman (NTCT 8178) was cultivated in 25 ml of tryptic soy broth (TSB) or brain heart infusion broth (BHI) (Oxoid, Denmark) in a 250-ml narrow-neck Erlenmeyer flask at 37°C and 200 rpm.

    Techniques: Electron Paramagnetic Resonance

    FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Journal: Applied and Environmental Microbiology

    Article Title: A Single Mechanosensitive Channel Protects Francisella tularensis subsp. holarctica from Hypoosmotic Shock and Promotes Survival in the Aquatic Environment

    doi: 10.1128/AEM.02203-17

    Figure Lengend Snippet: FTL_1753 is required for F. tularensis subsp. holarctica to survive hypoosmotic shock. (A and B) Survival data from downshock experiments performed in BHI broth. Bacteria were grown in BHI broth plus 300 mM NaCl, and then diluted in BHI broth plus 300, 200, 100, or 0 mM NaCl, producing hypoosmotic shocks of 0, 100, 200, and 300 mM, respectively. (C) Survival data for PBS-equilibrated bacteria diluted in water retrieved from a freshwater lake or double-distilled H 2 O. Statistical differences were determined by one-way analyses of variance (ANOVAs) with Dunnett's posttests. *, P

    Article Snippet: For downshock experiments conducted in medium, single colonies of each strain were picked from MH chocolate agar plates and grown in BHI broth supplemented with 300 mM NaCl (EMD Chemicals).

    Techniques:

    Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in BHI with serum at 37°C and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)

    Journal: FEMS microbiology letters

    Article Title: Expression of the Collagen Adhesin ace by Enterococcus faecalis Strain OG1RF is not Repressed by Ers but Requires the Ers box

    doi: 10.1111/1574-6968.12146

    Figure Lengend Snippet: Expression of ace/ Ace by E. faecalis OG1RF and its Δers derivative. (A) Transcriptional changes by strains grown in BHI with serum at 37°C and in BHI at 46°C. Northern blot analysis was performed with total RNA (gel, 10 µg/lane)

    Article Snippet: Bacteria were grown at 37°C in Luria-Bertani (LB) broth, Brain Heart Infusion (BHI) broth, M17 medium supplemented with 0.5% glucose (M17-G) or MM9YEG with 10 mM p -chloro-phenylalanine (p-Cl-Phe, Sigma-Aldrich Co.) ( ).

    Techniques: Expressing, Northern Blot

    Impaired growth of S. pneumoniae R6 strain EL1387 containing a regulated replacement of clpX (Δ clpX P fcsK :: clpX + ) on medium lacking fucose. (A) EL59 ( clpX + parent), EL1383 (regulated merodiploid clpX + P fcsK :: clpX + ), and EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) were streaked onto TSA-BA plates containing 0.25% (wt/vol) fucose (inducing) or lacking additional fucose (noninducing). Plates were photographed after 24 h of incubation at 37°C in an atmosphere of 5% CO 2 . (B) EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) was grown statically overnight in BHI broth containing 0.1% (wt/vol) fucose at 37°C in 5% CO 2 . Cultures were then diluted 100-fold into fresh BHI containing or lacking 0.1% (wt/vol) fucose, and static incubation was continued at 37°C in 5% CO 2 . Filled circles and squares represent the optical densities and viable cell counts, respectively, of the culture containing 0.1% (wt/vol) fucose. Open circles and squares represent the optical densities and viable cell counts, respectively, of the culture lacking fucose (0.001% [wt/vol]) carryover fucose from starting culture). Results are representative of at least two independent experiments. OD 620 (1.4 cm), optical density at 620 nm for a tube with a 1.4-cm diameter.

    Journal: Journal of Bacteriology

    Article Title: Essentiality of clpX, but Not clpP, clpL, clpC, or clpE, in Streptococcus pneumoniae R6

    doi: 10.1128/JB.185.9.2961-2966.2003

    Figure Lengend Snippet: Impaired growth of S. pneumoniae R6 strain EL1387 containing a regulated replacement of clpX (Δ clpX P fcsK :: clpX + ) on medium lacking fucose. (A) EL59 ( clpX + parent), EL1383 (regulated merodiploid clpX + P fcsK :: clpX + ), and EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) were streaked onto TSA-BA plates containing 0.25% (wt/vol) fucose (inducing) or lacking additional fucose (noninducing). Plates were photographed after 24 h of incubation at 37°C in an atmosphere of 5% CO 2 . (B) EL1387 (regulated replacement Δ clpX P fcsK :: clpX + ) was grown statically overnight in BHI broth containing 0.1% (wt/vol) fucose at 37°C in 5% CO 2 . Cultures were then diluted 100-fold into fresh BHI containing or lacking 0.1% (wt/vol) fucose, and static incubation was continued at 37°C in 5% CO 2 . Filled circles and squares represent the optical densities and viable cell counts, respectively, of the culture containing 0.1% (wt/vol) fucose. Open circles and squares represent the optical densities and viable cell counts, respectively, of the culture lacking fucose (0.001% [wt/vol]) carryover fucose from starting culture). Results are representative of at least two independent experiments. OD 620 (1.4 cm), optical density at 620 nm for a tube with a 1.4-cm diameter.

    Article Snippet: Ten independent exponential cultures of EL59 (R6 parent strain) in brain heart infusion (BHI) medium were diluted 1:20 in 1 ml of BHI medium containing 10% heat-inactivated horse serum (Sigma), 10 mM glucose, and 100 ng of competence stimulatory peptide 1 to give a cell density of ∼7.0 × 105 CFU/ml ( ).

    Techniques: Incubation

    Protease activation in the extracellular protein fraction from P. gingivalis FLL93. P. gingivalis was grown to stationary phase (OD 600 of 1.3) in 1 liter of BHI broth supplemented with hemin and vitamin K. Acetone (37.5%)-precipitated proteins were mixed with 8 M urea and were incubated at 4°C for 1 h. The urea was slowly removed from the mixture by centrifugation (10,000 × g ) in a Millipore filtration unit with the addition of increasing volumes of 100 mM Tris-HCl buffer (pH 7.4). Activities for Rgp and Kgp were tested. Error bars indicate the standard errors of the means of three independent trials.

    Journal: Infection and Immunity

    Article Title: The vimE Gene Downstream of vimA Is Independently Expressed and Is Involved in Modulating Proteolytic Activity in Porphyromonas gingivalis W83

    doi: 10.1128/IAI.72.10.5555-5564.2004

    Figure Lengend Snippet: Protease activation in the extracellular protein fraction from P. gingivalis FLL93. P. gingivalis was grown to stationary phase (OD 600 of 1.3) in 1 liter of BHI broth supplemented with hemin and vitamin K. Acetone (37.5%)-precipitated proteins were mixed with 8 M urea and were incubated at 4°C for 1 h. The urea was slowly removed from the mixture by centrifugation (10,000 × g ) in a Millipore filtration unit with the addition of increasing volumes of 100 mM Tris-HCl buffer (pH 7.4). Activities for Rgp and Kgp were tested. Error bars indicate the standard errors of the means of three independent trials.

    Article Snippet: P. gingivalis strains were grown in brain heart infusion (BHI) broth supplemented with 0.5% yeast extract (both from Difco Laboratories, Detroit, Mich.), hemin (5 μg/ml), vitamin K (0.5 μg/ml), and cysteine (0.1%) (all from Sigma-Aldrich, St. Louis, Mo.).

    Techniques: Activation Assay, Incubation, Centrifugation, Filtration

    Inactivation of vimE resulted in decreased proteolytic activity of P. gingivalis. P. gingivalis was grown to late log phase (OD 600 of 0.7 to 0.8) or stationary phase (OD 600 of 1.4 to 1.5) in BHI broth supplemented with yeast extract, hemin, and vitamin K. Activities against Rgp (A) and Kgp (B) were tested in whole-cell culture. The results shown are representative of three independent experiments in triplicate. E, exponential phase; L, stationary phase. Error bars indicate the standard errors of the means of three independent trials.

    Journal: Infection and Immunity

    Article Title: The vimE Gene Downstream of vimA Is Independently Expressed and Is Involved in Modulating Proteolytic Activity in Porphyromonas gingivalis W83

    doi: 10.1128/IAI.72.10.5555-5564.2004

    Figure Lengend Snippet: Inactivation of vimE resulted in decreased proteolytic activity of P. gingivalis. P. gingivalis was grown to late log phase (OD 600 of 0.7 to 0.8) or stationary phase (OD 600 of 1.4 to 1.5) in BHI broth supplemented with yeast extract, hemin, and vitamin K. Activities against Rgp (A) and Kgp (B) were tested in whole-cell culture. The results shown are representative of three independent experiments in triplicate. E, exponential phase; L, stationary phase. Error bars indicate the standard errors of the means of three independent trials.

    Article Snippet: P. gingivalis strains were grown in brain heart infusion (BHI) broth supplemented with 0.5% yeast extract (both from Difco Laboratories, Detroit, Mich.), hemin (5 μg/ml), vitamin K (0.5 μg/ml), and cysteine (0.1%) (all from Sigma-Aldrich, St. Louis, Mo.).

    Techniques: Activity Assay, Cell Culture

    Immunoreactivity of the RgpB proenzyme in P. gingivalis FLL93. Western blot analysis using specific RgpB proenzyme antibodies as probes was done on the extracellular fractions from P. gingivalis W83 (lane 1), FLL92 (lane 2), and FLL93 (lane 3). All lanes contained 20 μg of acetone (37.5%)-precipitated proteins from the supernatant fractions of cultures in BHI medium at exponential growth phase.

    Journal: Infection and Immunity

    Article Title: The vimE Gene Downstream of vimA Is Independently Expressed and Is Involved in Modulating Proteolytic Activity in Porphyromonas gingivalis W83

    doi: 10.1128/IAI.72.10.5555-5564.2004

    Figure Lengend Snippet: Immunoreactivity of the RgpB proenzyme in P. gingivalis FLL93. Western blot analysis using specific RgpB proenzyme antibodies as probes was done on the extracellular fractions from P. gingivalis W83 (lane 1), FLL92 (lane 2), and FLL93 (lane 3). All lanes contained 20 μg of acetone (37.5%)-precipitated proteins from the supernatant fractions of cultures in BHI medium at exponential growth phase.

    Article Snippet: P. gingivalis strains were grown in brain heart infusion (BHI) broth supplemented with 0.5% yeast extract (both from Difco Laboratories, Detroit, Mich.), hemin (5 μg/ml), vitamin K (0.5 μg/ml), and cysteine (0.1%) (all from Sigma-Aldrich, St. Louis, Mo.).

    Techniques: Western Blot

    Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. monocytogenes in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on BHI agar plate.

    Journal: PLoS ONE

    Article Title: Protective Role of Heme Oxygenase-1 in Listeria monocytogenes-Induced Abortion

    doi: 10.1371/journal.pone.0025046

    Figure Lengend Snippet: Prevention of infectious abortion by HO-1 and Bcl-XL expression. (A) Pregnant mice were infected with 10 5 cells of L. monocytogenes in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on BHI agar plate.

    Article Snippet: Bacterial strains L. monocytogenes EGD was maintained as a frozen glycerol stock and cultured in brain heart infusion (BHI) broth (Becton Dickinson) or on BHI broth containing 1.5% agar.

    Techniques: Expressing, Mouse Assay, Infection

    Decreased HO-1 and Bcl-XL expression in TG cells infected with L. monocytogenes . (A) TG cells were first treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 6 h, HO-1 and Bcl-XL expression was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) TG cells were treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 0.5, 2, and 6 h incubation, the infected cells were washed with PBS and lysed with cold distilled water. CFU were determined by serial dilution on BHI agar plates. (C) L. monocytogenes was deposited on TG cells by centrifugation at 150×g for 10 min at room temperature, incubated for 6 h, fixed, and stained. The figure shows FITC-labeled bacteria (green) and Alexa Fluor 594-labeled actin filaments (red) merged images. The left-hand panel shows untreated cells, the center panel Co-PP (9 µg/ml)-treated cells, and the right-hand panel, cytochalasin D-treated cells.

    Journal: PLoS ONE

    Article Title: Protective Role of Heme Oxygenase-1 in Listeria monocytogenes-Induced Abortion

    doi: 10.1371/journal.pone.0025046

    Figure Lengend Snippet: Decreased HO-1 and Bcl-XL expression in TG cells infected with L. monocytogenes . (A) TG cells were first treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 6 h, HO-1 and Bcl-XL expression was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) TG cells were treated with Co-PP and then infected with L. monocytogenes . The infected cells were cultured in 50 µg/ml of gentamicin. After 0.5, 2, and 6 h incubation, the infected cells were washed with PBS and lysed with cold distilled water. CFU were determined by serial dilution on BHI agar plates. (C) L. monocytogenes was deposited on TG cells by centrifugation at 150×g for 10 min at room temperature, incubated for 6 h, fixed, and stained. The figure shows FITC-labeled bacteria (green) and Alexa Fluor 594-labeled actin filaments (red) merged images. The left-hand panel shows untreated cells, the center panel Co-PP (9 µg/ml)-treated cells, and the right-hand panel, cytochalasin D-treated cells.

    Article Snippet: Bacterial strains L. monocytogenes EGD was maintained as a frozen glycerol stock and cultured in brain heart infusion (BHI) broth (Becton Dickinson) or on BHI broth containing 1.5% agar.

    Techniques: Expressing, Infection, Cell Culture, Incubation, Serial Dilution, Centrifugation, Staining, Labeling

    Prevention of cell death by HO-1 and Bcl-XL expression. (A) TG cells were treated for 48 h with either siRNA targeting HO-1, Bcl-XL, or control siRNA (QIAGEN AllStars Negative Control). Bcl-XL overexpression was achieved by transfecting the cells with pcDNA4/TO-Bcl-XL. HO-1 and Bcl-XL expression was monitored by immunoblotting. β-actin was used as an internal control. A representative immunoblot of three independent experiments is shown. (B) TG cells were infected with L. monocytogenes . The infected cells were cultured with media containing 50 µg/ml gentamicin for 2, 6, and 12 h. The cells were then washed with PBS and lysed with cold distilled water. CFU was determined by serial dilution on BHI agar plates. All values represent the average and the standard deviation of three identical experiments. (C) Cell death was determined using the JC-1 Mitochondrial Membrane Potential Assay Kit. One hundred TG cells per coverslip were examined to determine the total number of live or dead cells. All values represent the average and the standard deviation of three identical experiments. Statistically significant differences compared with the control are indicated by asterisks (*, P

    Journal: PLoS ONE

    Article Title: Protective Role of Heme Oxygenase-1 in Listeria monocytogenes-Induced Abortion

    doi: 10.1371/journal.pone.0025046

    Figure Lengend Snippet: Prevention of cell death by HO-1 and Bcl-XL expression. (A) TG cells were treated for 48 h with either siRNA targeting HO-1, Bcl-XL, or control siRNA (QIAGEN AllStars Negative Control). Bcl-XL overexpression was achieved by transfecting the cells with pcDNA4/TO-Bcl-XL. HO-1 and Bcl-XL expression was monitored by immunoblotting. β-actin was used as an internal control. A representative immunoblot of three independent experiments is shown. (B) TG cells were infected with L. monocytogenes . The infected cells were cultured with media containing 50 µg/ml gentamicin for 2, 6, and 12 h. The cells were then washed with PBS and lysed with cold distilled water. CFU was determined by serial dilution on BHI agar plates. All values represent the average and the standard deviation of three identical experiments. (C) Cell death was determined using the JC-1 Mitochondrial Membrane Potential Assay Kit. One hundred TG cells per coverslip were examined to determine the total number of live or dead cells. All values represent the average and the standard deviation of three identical experiments. Statistically significant differences compared with the control are indicated by asterisks (*, P

    Article Snippet: Bacterial strains L. monocytogenes EGD was maintained as a frozen glycerol stock and cultured in brain heart infusion (BHI) broth (Becton Dickinson) or on BHI broth containing 1.5% agar.

    Techniques: Expressing, Negative Control, Over Expression, Infection, Cell Culture, Serial Dilution, Standard Deviation

    Growth-dependent bioluminescence in phage-infected B. anthracis cells (A) Overnight Sterne cultures were diluted (1:100) with BHI broth and incubated at 35°C with continuous shaking (250 rpm) and assessed at various times for A 600 . At the times indicated (denoted by the arrows), samples were harvested and mixed with 10 9 PFU mL −1 (final concentration) phage and bioluminescence was determined after 1 h (B) . Numbers indicate the mean (n=3) ± SD. *p

    Journal: Journal of applied microbiology

    Article Title: Detection of Bacillus anthracis Spores from Environmental Water Using Bioluminescent Reporter Phage

    doi: 10.1111/jam.13569

    Figure Lengend Snippet: Growth-dependent bioluminescence in phage-infected B. anthracis cells (A) Overnight Sterne cultures were diluted (1:100) with BHI broth and incubated at 35°C with continuous shaking (250 rpm) and assessed at various times for A 600 . At the times indicated (denoted by the arrows), samples were harvested and mixed with 10 9 PFU mL −1 (final concentration) phage and bioluminescence was determined after 1 h (B) . Numbers indicate the mean (n=3) ± SD. *p

    Article Snippet: All strains ( B. anthracis and B. cereus ) were grown in BD™ Brain Heart Infusion broth (BHI), soft agar (broth and 0.7% Bacto agar) or broth plus 1.5% agar.

    Techniques: Infection, Incubation, Concentration Assay

    Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. monocytogenes EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in BHI from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl

    Journal: Bioengineered Bugs

    Article Title: Development and optimization of an EGFP-based reporter for measuring the general stress response in Listeria monocytogenes

    doi: 10.4161/bbug.19476

    Figure Lengend Snippet: Figure 5. σ B -dependent EGFP expression is induced after osmotic upshock in L. monocytogenes EGD-e wild-type P lmo2230 ::egfp integrant. Cells bearing P lmo2230 ::egfp fusion grown in BHI from OD 600 = 0.05 up to OD 600 = 0.6 when 0.5 M NaCl

    Article Snippet: L. monocytogenes strains were grown in Brain Heart Infusion (BHI) broth or agar (LabM) at 37°C unless otherwise stated.

    Techniques: Expressing

    Correlation of bioluminescence (relative light units [RLU]) and culture regimen-dependent viable cell count (CFU per square centimeter) measured at 10°C. The A lines show the correlation on cheese after 14 days of storage (open squares) and during the first 3 days after inoculation (closed squares). The B lines show the correlation for Y . enterocolitica on BHI agar with 2.5% salt (closed triangles) and without salt (open triangles) on the first 5 days. The background luminescence of cheese and BHI agar has been subtracted. Immediately after the light intensity was measured, the plates or cheeses were used to determine the viable counts (see text).

    Journal: Applied and Environmental Microbiology

    Article Title: Sensitive In Situ Monitoring of a Recombinant Bioluminescent Yersinia enterocolitica Reporter Mutant in Real Time on Camembert Cheese

    doi: 10.1128/AEM.68.11.5737-5740.2002

    Figure Lengend Snippet: Correlation of bioluminescence (relative light units [RLU]) and culture regimen-dependent viable cell count (CFU per square centimeter) measured at 10°C. The A lines show the correlation on cheese after 14 days of storage (open squares) and during the first 3 days after inoculation (closed squares). The B lines show the correlation for Y . enterocolitica on BHI agar with 2.5% salt (closed triangles) and without salt (open triangles) on the first 5 days. The background luminescence of cheese and BHI agar has been subtracted. Immediately after the light intensity was measured, the plates or cheeses were used to determine the viable counts (see text).

    Article Snippet: They were grown in brain heart infusion (BHI) broth (Merck) supplemented with 2.5% NaCl (10°C, 48 h).

    Techniques: Cell Counting

    Influence of growth temperature and growth phase on bioluminescence of Y . enterocolitica B94. The mutant was grown in BHI broth with 2.5% salt at different temperatures to an OD 600 of 0.15 (5 × 10 7 CFU/ml) (triangles) and to an OD 600 of 1.1 (4.5 × 10 8 CFU/ml) (diamonds). A 100-μl sample of the grown culture was spread on BHI plates preadjusted to 10°C. After 5 min at 10°C, bioluminescence was measured at 10°C. RLU, relative light units; 1.0E+02, 1 × 10 2 .

    Journal: Applied and Environmental Microbiology

    Article Title: Sensitive In Situ Monitoring of a Recombinant Bioluminescent Yersinia enterocolitica Reporter Mutant in Real Time on Camembert Cheese

    doi: 10.1128/AEM.68.11.5737-5740.2002

    Figure Lengend Snippet: Influence of growth temperature and growth phase on bioluminescence of Y . enterocolitica B94. The mutant was grown in BHI broth with 2.5% salt at different temperatures to an OD 600 of 0.15 (5 × 10 7 CFU/ml) (triangles) and to an OD 600 of 1.1 (4.5 × 10 8 CFU/ml) (diamonds). A 100-μl sample of the grown culture was spread on BHI plates preadjusted to 10°C. After 5 min at 10°C, bioluminescence was measured at 10°C. RLU, relative light units; 1.0E+02, 1 × 10 2 .

    Article Snippet: They were grown in brain heart infusion (BHI) broth (Merck) supplemented with 2.5% NaCl (10°C, 48 h).

    Techniques: Mutagenesis