Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Growth conditions and environmental factors impact aerosolization but not virulence of Francisella tularensis infection in mice
Figure Lengend Snippet: Bacterial load in tissues of mice after aerosol exposure to LVS grown in different culture media. Mice became moribund after LVS infection were euthanized and necropsied. The liver, lung, and spleen were removed, weighed, and then analyzed to determine the bacterial titer in each tissue. Graphs in (A,B) show weight for lungs (A) and spleens (B) at time of necropsy from individual mice infected with LVS grown in MHb, CCDM, or BHI as well as the mean for each group and standard deviation. Weights of tissues from naive, uninfected mice are also shown. Graph in (C) shows a box and whiskers plot for the bacterial titer from the lung, liver, and spleen of mice that succumbed to infection with LVS grown in MHb (white boxes), CCDM (light gray boxes), or BHI (dark gray boxes).
Article Snippet: Broth media Three different broth media were evaluated in these studies: (1) modified Mueller–Hinton medium (MHb) supplemented with 1.23 mM calcium chloride dihydrate, 1.03 mM magnesium chloride hexahydrate, 0.1% (wt/vol) glucose, 0.025% (wt/vol) ferric pyrophosphate, and 2% (vol/vol) IsoVitaleX (Becton-Dickinson, Franklin Lakes, NJ); (2) Chamberlain's chemically defined medium (CCDM) (Chamberlain, ); or (3) brain heart infusion (BHI) broth (Becton-Dickinson, Franklin Lakes, NJ).
Techniques: Mouse Assay, Infection, Standard Deviation