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  • 99
    Thermo Fisher bromouridine 5 triphosphate brutp
    npLa accumulates in nucleoli and colocalizes with fibrillarin and newly synthesized RNA polymerase I transcripts. Normal human fibroblasts (HSC172) were incubated with AbNP and with either antifibrillarin (A to C) or anti-B23 (D to F). AbNP is represented in red (A and D), and antifibrillarin and anti-B23 (fluorescein isothiocyanate) are represented in green (B and E). Although these colocalizations were done in parallel, the images in panels A to C are intentionally shown at higher magnification than those in panels D to F. Panels A and B were overlaid to produce panel C, and panels D and E were overlaid to produce panel F. (G to I) <t>Anti-BrUTP</t> and npLa are visualized in the red and green channels, respectively, by confocal microscopy, and the red and green images were overlaid to visualize colocalization. BrUTP incorporation occurred in the presence of 0 (G), 2 (H), and 100 (I) μg of <t>α-amanitin/ml.</t>
    Bromouridine 5 Triphosphate Brutp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 5 bromouridine 5 triphosphate brutp
    Rapid Genome-wide Transcriptional Reprogramming in the Absence of Protein Synthesis (A) Experimental design for the <t>RNA-seq</t> analysis of newly transcribed mRNAs before and after NT to Xenopus oocytes. <t>BrUTP</t> is used to label newly transcribed RNAs. (B) Venn diagram of genes classified as activated (reprogrammed), continuously transcribed (maintained), or repressed (downregulated) after NT based on log 2 count per million (log 2 CPM donor cell divided by NT). False discovery rate (FDR)
    5 Bromouridine 5 Triphosphate Brutp, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore brutp
    Rapid Genome-wide Transcriptional Reprogramming in the Absence of Protein Synthesis (A) Experimental design for the <t>RNA-seq</t> analysis of newly transcribed mRNAs before and after NT to Xenopus oocytes. <t>BrUTP</t> is used to label newly transcribed RNAs. (B) Venn diagram of genes classified as activated (reprogrammed), continuously transcribed (maintained), or repressed (downregulated) after NT based on log 2 count per million (log 2 CPM donor cell divided by NT). False discovery rate (FDR)
    Brutp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim br utp
    Rapid Genome-wide Transcriptional Reprogramming in the Absence of Protein Synthesis (A) Experimental design for the <t>RNA-seq</t> analysis of newly transcribed mRNAs before and after NT to Xenopus oocytes. <t>BrUTP</t> is used to label newly transcribed RNAs. (B) Venn diagram of genes classified as activated (reprogrammed), continuously transcribed (maintained), or repressed (downregulated) after NT based on log 2 count per million (log 2 CPM donor cell divided by NT). False discovery rate (FDR)
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    Thermo Fisher mouse anti brutp
    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and <t>LB1.</t> Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by <t>BrUTP</t> incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
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    Millipore 5 bromo uridine triphosphate brutp
    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and <t>LB1.</t> Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by <t>BrUTP</t> incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.
    5 Bromo Uridine Triphosphate Brutp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti br utp mab
    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of <t>Br-UTP,</t> and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of <t>hnRNP</t> A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    Anti Br Utp Mab, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti brutp
    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of <t>Br-UTP,</t> and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of <t>hnRNP</t> A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    Anti Brutp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher br utp
    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of <t>Br-UTP,</t> and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of <t>hnRNP</t> A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    Br Utp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bromo utp br utp
    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of <t>Br-UTP,</t> and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of <t>hnRNP</t> A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    Bromo Utp Br Utp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore triphosphate sodium salt br utp sigma 100 mm in 2 mm pipes buffer
    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of <t>Br-UTP,</t> and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of <t>hnRNP</t> A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    Triphosphate Sodium Salt Br Utp Sigma 100 Mm In 2 Mm Pipes Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore 5 bromouridine 5 triphosphato brutp
    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of <t>Br-UTP,</t> and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of <t>hnRNP</t> A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    5 Bromouridine 5 Triphosphato Brutp, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher m br utp
    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of <t>Br-UTP,</t> and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of <t>hnRNP</t> A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    M Br Utp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    5 Bromouridine 5 triphosphate 5 BrUTP is a brominated form of UTP that is used to label RNA during transcription 5 BrUTP in newly transcribed RNA is then evaluated immunologically
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    N/A
    5 Bromouridine 5 triphosphate sodium salt is a nucleotide analog of uridine It is used to measure transcription via labeling of ribonucleic acids RNA
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    N/A
    5 Bromo 2 deoxyuridine 5 triphosphate sodium salt 5 BrdUTP is a building block for preparing BrdU containing nucleotides It is effective as a mutagenic agent and may be used
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    Image Search Results


    npLa accumulates in nucleoli and colocalizes with fibrillarin and newly synthesized RNA polymerase I transcripts. Normal human fibroblasts (HSC172) were incubated with AbNP and with either antifibrillarin (A to C) or anti-B23 (D to F). AbNP is represented in red (A and D), and antifibrillarin and anti-B23 (fluorescein isothiocyanate) are represented in green (B and E). Although these colocalizations were done in parallel, the images in panels A to C are intentionally shown at higher magnification than those in panels D to F. Panels A and B were overlaid to produce panel C, and panels D and E were overlaid to produce panel F. (G to I) Anti-BrUTP and npLa are visualized in the red and green channels, respectively, by confocal microscopy, and the red and green images were overlaid to visualize colocalization. BrUTP incorporation occurred in the presence of 0 (G), 2 (H), and 100 (I) μg of α-amanitin/ml.

    Journal: Molecular and Cellular Biology

    Article Title: Nonphosphorylated Human La Antigen Interacts with Nucleolin at Nucleolar Sites Involved in rRNA Biogenesis

    doi: 10.1128/MCB.24.24.10894-10904.2004

    Figure Lengend Snippet: npLa accumulates in nucleoli and colocalizes with fibrillarin and newly synthesized RNA polymerase I transcripts. Normal human fibroblasts (HSC172) were incubated with AbNP and with either antifibrillarin (A to C) or anti-B23 (D to F). AbNP is represented in red (A and D), and antifibrillarin and anti-B23 (fluorescein isothiocyanate) are represented in green (B and E). Although these colocalizations were done in parallel, the images in panels A to C are intentionally shown at higher magnification than those in panels D to F. Panels A and B were overlaid to produce panel C, and panels D and E were overlaid to produce panel F. (G to I) Anti-BrUTP and npLa are visualized in the red and green channels, respectively, by confocal microscopy, and the red and green images were overlaid to visualize colocalization. BrUTP incorporation occurred in the presence of 0 (G), 2 (H), and 100 (I) μg of α-amanitin/ml.

    Article Snippet: Cells were washed, permeabilized, and incubated for 10 min at 37°C in transcription buffer containing 0.5 mM 5-bromouridine 5′-triphosphate (BrUTP; Sigma) containing the appropriate concentration of α-amanitin and processed as described previously with primary monoclonal Ab against BrUTP (1:200; Caltag) and AbNP (1:50) in phosphate-buffered saline for 1 h, washed with phosphate-buffered saline, and incubated with secondary Ab conjugated with fluorescein or Texas red (Vector Laboratories) ( , ).

    Techniques: Synthesized, Incubation, Confocal Microscopy

    Rapid Genome-wide Transcriptional Reprogramming in the Absence of Protein Synthesis (A) Experimental design for the RNA-seq analysis of newly transcribed mRNAs before and after NT to Xenopus oocytes. BrUTP is used to label newly transcribed RNAs. (B) Venn diagram of genes classified as activated (reprogrammed), continuously transcribed (maintained), or repressed (downregulated) after NT based on log 2 count per million (log 2 CPM donor cell divided by NT). False discovery rate (FDR)

    Journal: Molecular Cell

    Article Title: Hierarchical Molecular Events Driven by Oocyte-Specific Factors Lead to Rapid and Extensive Reprogramming

    doi: 10.1016/j.molcel.2014.06.024

    Figure Lengend Snippet: Rapid Genome-wide Transcriptional Reprogramming in the Absence of Protein Synthesis (A) Experimental design for the RNA-seq analysis of newly transcribed mRNAs before and after NT to Xenopus oocytes. BrUTP is used to label newly transcribed RNAs. (B) Venn diagram of genes classified as activated (reprogrammed), continuously transcribed (maintained), or repressed (downregulated) after NT based on log 2 count per million (log 2 CPM donor cell divided by NT). False discovery rate (FDR)

    Article Snippet: RNA Immunoprecipitation BrUTP (Sigma: B7166, 4.6 nl of 100 mM stock) was injected to the cytoplasm of Xenopus oocytes 2 hr after transplantation with MEFs.

    Techniques: Genome Wide, RNA Sequencing Assay

    Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Journal: Nucleus

    Article Title: Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells

    doi: 10.1080/19491034.2015.1004256

    Figure Lengend Snippet: Bleb-associated genetic regions are actively transcribed. ( A ) Primary p.S143F fibroblasts (p33) were stained for either RNA polymerase II (first row) or active RNA polymerase II (Pol IIo, second row), LA and LB1. Hoechst was used to visualize DNA. Single mid-plane confocal sections from the whole cell are shown. RNA polymerase II and active RNA polymerase II (Pol IIo) are enriched in the blebs (arrows). Scale bars 5 μm. ( B ) Transcriptional activity in p.S143F fibroblasts (p30) was visualized by BrUTP incorporation into nascent RNA by scratch labeling. DAPI was used to visualize DNA. A single mid-plane confocal section is shown. Note the intense BrUTP staining in the bleb compartment (arrow). Scale bar 5 μm. ( C ) p.S143F fibroblasts (p36) were subjected to RNA FISH with a probe for a highly transcribed region on chromosome 19. DAPI was used to visualize DNA. Single confocal sections from mid-plane and nuclear surface are shown. FISH signals are detectable in the bleb region (arrow). Scale bar 5 μm. ( D ) p.S143F fibroblasts (p21) were stained for LA/C and histone H3 trimethylated at lysine 4 or lysine 36. Hoechst was used to visualize DNA. Single mid-plane confocal sections are shown. Both histone marks are detectable in the bleb region (arrows). Scale bars 5 μm.

    Article Snippet: Primary antibodies used were mouse monoclonal anti-LA/C (1:300; JoL2, Chemicon, cat.# MAB3211), rabbit polyclonal anti-LA (1:1000) , rabbit polyclonal anti-LB1 (1:1000) , mouse monoclonal anti-LB2 (1:100; LN43, Abcam, cat.# ab8983), rabbit polyclonal anti-H3K9me3 (1:1000, Abcam, cat.# ab8898) and rabbit polyclonal anti-H3K27me3 (1:1000, Abcam, cat.# ab108245), rabbit polyclonal anti-H3K4me2 (1:1000, Abcam, cat.# ab7766), rabbit polyclonal anti-H3K36me3 (1:1000, Abcam, cat.# ab9050, rabbit polyclonal anti-AcH3 (1:1000, Upstate/Merck Millipore, cat.# 06–599), mouse monoclonal anti-Pol II (1:1000, 4H8, Abcam, cat.# ab5408), mouse monoclonal anti-Pol II phosphorylated at Ser2 (H5, 1:100, Abcam, cat.# ab24758), mouse monoclonal anti-FLAG (1:400, clone M2, Sigma, cat.# F1804), rabbit polyclonal anti-SUN1 and anti-SUN2 (1:40 and 1:20; kindly provided by Didier Hodzic) , goat polyclonal anti-LB1 (1:200 Santa Cruz cat.# sc-6217), mouse anti-BrUTP (1:100, Caltag Laboratories, clone Br-3), rabbit polyclonal anti-p53 (1:100, Imgenex, cat.# IMG-533) and rabbit polyclonal anti-SKIP (NCOA62) (1:100, Abcam, cat.# ab153887).

    Techniques: Staining, Activity Assay, Labeling, Fluorescence In Situ Hybridization

    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of Br-UTP, and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of hnRNP A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.

    Journal: Molecular Biology of the Cell

    Article Title: Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription

    doi:

    Figure Lengend Snippet: ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of Br-UTP, and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of hnRNP A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.

    Article Snippet: Antibodies used in this study included anti-UBF–specific human serum at a dilution of 1:300 ( ; ), anti-nucleolin mAb at a dilution of 1:3000 , anti-fibrillarin human serum at a dilution of 1:100 , anti-SC35 at a dilution of 1:1000 , anti-hnRNP A1 at a dilution of 1:300 , and anti-Br-UTP mAb at a dilution of 1:500 (Sigma Chemical, St. Louis, MO) for 1 h at room temperature.

    Techniques: Concentration Assay, In Situ, Immunolabeling, Labeling