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  • 89
    Illumina Inc 100 bp paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    100 Bp Paired End Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Illumina Inc bp paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Bp Paired End Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 89/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc 150 bp paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    150 Bp Paired End Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc 76 bp paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    76 Bp Paired End Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novogene 150 bp paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    150 Bp Paired End Mode, supplied by Novogene, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Illumina Inc 250 bp paired end mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    250 Bp Paired End Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eurofins 150 bp paired end read mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    150 Bp Paired End Read Mode, supplied by Eurofins, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc 150 bp paired end reads mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    150 Bp Paired End Reads Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Shanghai Biochip Co. Ltd 150 bp paired end
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    150 Bp Paired End, supplied by Shanghai Biochip Co. Ltd, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc paired end 125 bp mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Paired End 125 Bp Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc 100 bp paired end protocol
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    100 Bp Paired End Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Illumina Inc paired end 2 × 300 bp mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Paired End 2 × 300 Bp Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Illumina Inc paired end 75 bp mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Paired End 75 Bp Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc hiseq2500 rapid mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Hiseq2500 Rapid Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Illumina Inc 125 bp paired end sequencing platform
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    125 Bp Paired End Sequencing Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Illumina Inc multiplex illumina hiseq high output mode
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Multiplex Illumina Hiseq High Output Mode, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc hiseq 2500 platform
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Hiseq 2500 Platform, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 19633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Elim Bio illumina hiseq 2500
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Illumina Hiseq 2500, supplied by Elim Bio, used in various techniques. Bioz Stars score: 91/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc illumina hiseq 1500
    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, <t>100</t> μm wide field). Error bars, SEM; *p
    Illumina Hiseq 1500, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 1121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, 100 μm wide field). Error bars, SEM; *p

    Journal: eLife

    Article Title: Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development

    doi: 10.7554/eLife.18683

    Figure Lengend Snippet: Changes in the proportion of cortical NSPC subtypes and neurons during human and chimpanzee cerebral organoid development. ( A ) Cryosections of cortical regions from human and chimpanzee organoids at day 28 and day 52 immunostained for PAX6 (magenta) and TBR2 (green) combined with DAPI staining. Scale bars; D28, 10 μm; D52, 20 μm. Insets in the D52 merge images show selected areas with PAX6+TBR2+ double-positive nuclei (arrowheads) at higher magnification. ( B ) Quantification of the percentage of PAX6+TBR2–, PAX6+TBR2+, PAX6–TBR2+ and PAX6–TBR2– cortical cells in human (light grey) and chimpanzee (dark grey) organoids at D28 (n = 5 organoids, 50 μm wide field) and D52-D54 (n = 17 organoids, 100 μm wide field). Error bars, SEM; *p

    Article Snippet: Up to 192 cells were pooled and sequenced in 100-bp paired-end mode on one lane of an Illumina HiSeq 2500 platform (rapid mode).

    Techniques: Staining

    Cell cycle assignment for differential gene expression analysis. ( A ) Hierarchical clustering was used to identify human organoid APs that most strongly expressed genes enriched in G2M phase of the cell cycle (red). The genes were identified from PCA on fetal cortex progenitor cells (top 100 correlating genes) ( Camp et al., 2015 ). The cluster with weakest expression of these G2M associated genes was assigned as G1 phase (blue). Intermediate cells (grey) were discarded from differential gene expression analysis. ( B ) A previously published method was used to computationally assign cell-cycle stage based on a machine-learning approach ( Scialdone et al., 2015 ). This method was generally consistent with our assignment based on the hierarchical clustering presented in panel A . ( C – F ) The same approach was used to identify the chimpanzee organoid APs, endothelial cells (ECs), and iPSCs that most highly express G2M markers. Note that all iPSCs analyzed highly expressed most of the G2M marker genes. DOI: http://dx.doi.org/10.7554/eLife.18683.026

    Journal: eLife

    Article Title: Differences and similarities between human and chimpanzee neural progenitors during cerebral cortex development

    doi: 10.7554/eLife.18683

    Figure Lengend Snippet: Cell cycle assignment for differential gene expression analysis. ( A ) Hierarchical clustering was used to identify human organoid APs that most strongly expressed genes enriched in G2M phase of the cell cycle (red). The genes were identified from PCA on fetal cortex progenitor cells (top 100 correlating genes) ( Camp et al., 2015 ). The cluster with weakest expression of these G2M associated genes was assigned as G1 phase (blue). Intermediate cells (grey) were discarded from differential gene expression analysis. ( B ) A previously published method was used to computationally assign cell-cycle stage based on a machine-learning approach ( Scialdone et al., 2015 ). This method was generally consistent with our assignment based on the hierarchical clustering presented in panel A . ( C – F ) The same approach was used to identify the chimpanzee organoid APs, endothelial cells (ECs), and iPSCs that most highly express G2M markers. Note that all iPSCs analyzed highly expressed most of the G2M marker genes. DOI: http://dx.doi.org/10.7554/eLife.18683.026

    Article Snippet: Up to 192 cells were pooled and sequenced in 100-bp paired-end mode on one lane of an Illumina HiSeq 2500 platform (rapid mode).

    Techniques: Expressing, Marker