Journal: Cell Death and Differentiation
Article Title: A novel role for the apoptosis inhibitor ARC in suppressing TNFα-induced regulated necrosis
Figure Lengend Snippet: The ARC CARD is required for the ability of ARC to suppress TNF α -induced necrosis. ( a ) Immunoblot showing inhibition of TNF α -induced HMGB1 release by wild-type ARC but not by the CARD-defective double-point ARC mutant (L31F; G69R). L929 cells were stably transduced with empty vector (Φ), ARC-HA (ARC) or ARC-HA double-point CARD mutant (DM). Immunblot of bovine serum albumin (BSA) in media used as loading control. ( b ) Inhibition of TNF α -induced LDH release by ARC requires the CARD. Data shown as mean±S.E. from n =4. *** P -value
Article Snippet: Primary antibodies include: ARC (Cayman Chemical, Ann Arbor, MI, USA); β -actin (Sigma-Aldrich, St. Louis, MO, USA); HA (Roche Applied Science, Indianapolis, IN, USA); BSA, HMGB1, His (Abcam, Cambridge, MA, USA); histone H3, PARP, p65 (Cell Signaling Technology, Danvers, MA, USA); RhoGDI α (A-20) (Santa Cruz Biotechnology, Dallas, TX, USA); mouse RIP3 (Prosci, Poway, CA, USA); human RIP3 (Thermo Scientific); RIP1 (BD Biosciences, San Jose, CA, USA); and FADD (Enzo Life Sciences, Farmingdale, NY, USA).
Techniques: Inhibition, Mutagenesis, Stable Transfection, Transduction, Plasmid Preparation