bovine pituitary extract Search Results


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  • 97
    Lonza bovine pituitary extract
    Bovine Pituitary Extract, supplied by Lonza, used in various techniques. Bioz Stars score: 97/100, based on 532 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine pituitary extract/product/Lonza
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    99
    Thermo Fisher bpe
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bpe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 346 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biomedical Technologies bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Biomedical Technologies, used in various techniques. Bioz Stars score: 92/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cambrex bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Cambrex, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    ScienCell bpe
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bpe, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher recombinant epidermal growth factor bpe regf
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Recombinant Epidermal Growth Factor Bpe Regf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher keratinocyte sfm
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Keratinocyte Sfm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Gemini Bio bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PromoCell bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by PromoCell, used in various techniques. Bioz Stars score: 92/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Kurabo industries bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pel-Freez bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Pel-Freez, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Scientific Cell Company bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Scientific Cell Company, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Upstate Biotechnology Inc bovine pituitary extract
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Bovine Pituitary Extract, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Avanti Polar porcine brain l α phosphatidylethanolamine bpe
    Influence of <t>EGF</t> and <t>BPE</t> on Gene Expression in Human Meibomian Gland Epithelial Cells
    Porcine Brain L α Phosphatidylethanolamine Bpe, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Expressing

    Effect of EGF + BPE, serum, and media on the proliferation of human meibomian gland epithelial cells. Cells ( n = 3 wells/condition) at passages 44 ( A ) and 49 ( B ) were cultured as described in the text. Values equal the mean ± SE. *Significantly

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Effect of EGF + BPE, serum, and media on the proliferation of human meibomian gland epithelial cells. Cells ( n = 3 wells/condition) at passages 44 ( A ) and 49 ( B ) were cultured as described in the text. Values equal the mean ± SE. *Significantly

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Cell Culture

    Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Expressing

    Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Expressing

    Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Expressing

    Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Expressing

    Effect of EGF + BPE, and serum on lipid accumulation in human meibomian gland and conjunctival epithelial cells. Cells were cultured in SFM or in media supplemented with EGF + BPE or serum. Cells were fixed and stained with LipidTox Green neutral lipid

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Effect of EGF + BPE, and serum on lipid accumulation in human meibomian gland and conjunctival epithelial cells. Cells were cultured in SFM or in media supplemented with EGF + BPE or serum. Cells were fixed and stained with LipidTox Green neutral lipid

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Cell Culture, Staining

    Impact of EGF, BPE, and EGF + BPE on the proliferation of human meibomian gland epithelial cells. Cells ( n = 3 wells/condition) at passages 50 ( A ) and 16 ( B ) were cultured as described in the text. Values equal the mean ± SE. Significantly (*

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Impact of EGF, BPE, and EGF + BPE on the proliferation of human meibomian gland epithelial cells. Cells ( n = 3 wells/condition) at passages 50 ( A ) and 16 ( B ) were cultured as described in the text. Values equal the mean ± SE. Significantly (*

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Cell Culture

    Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Expressing

    Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Expressing

    Influence of EGF, BPE, and EGF + BPE on the proliferation of human meibomian gland epithelial cells. Cells at passage 50 were cultured as explained in the text. Values represent the mean ± SE. *Significantly ( P

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

    doi: 10.1167/iovs.12-11221

    Figure Lengend Snippet: Influence of EGF, BPE, and EGF + BPE on the proliferation of human meibomian gland epithelial cells. Cells at passage 50 were cultured as explained in the text. Values represent the mean ± SE. *Significantly ( P

    Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

    Techniques: Cell Culture

    Effect of physiologic doses of pilocarpine or timolol on IHMGEC survival and proliferation. (a,b) IHMGECs (n = 3 wells/treatment/experiment) were treated with 0.001% pilocarpine, 0.0002% timolol, or vehicle for 24 hours. A representative of three separate experiments is shown. (c,d) Cells were cultured with 0.001% pilocarpine or 0.0002% timolol for 5 days in KSFM with or without EGF+BPE. A representative example of three experiments is shown. ** indicates p

    Journal: Cornea

    Article Title: Influence of pilocarpine and timolol on human meibomian gland epithelial cells

    doi: 10.1097/ICO.0000000000001181

    Figure Lengend Snippet: Effect of physiologic doses of pilocarpine or timolol on IHMGEC survival and proliferation. (a,b) IHMGECs (n = 3 wells/treatment/experiment) were treated with 0.001% pilocarpine, 0.0002% timolol, or vehicle for 24 hours. A representative of three separate experiments is shown. (c,d) Cells were cultured with 0.001% pilocarpine or 0.0002% timolol for 5 days in KSFM with or without EGF+BPE. A representative example of three experiments is shown. ** indicates p

    Article Snippet: Immortalized (I) HMGECs were cultured in keratinocyte serum-free medium (KSFM), supplemented with 5 ng/ml epidermal growth factor (EGF) and 50 μg/ml bovine pituitary extract (BPE) (Life Technologies, Grand Island, NY).

    Techniques: Cell Culture

    Influence of clinical doses of pilocarpine or timolol on IHMGEC survival. IHMGECs (n = 3 wells/treatment/experiment) were treated with various concentrations of anti-glaucoma drugs, or EGF+BPE or vehicle for 24 hours. (a) Pilocarpine and timolol induce time- and dose-dependent morphological changes in IHMGECs. Images shown are representative of three separate experiments. All images are 200X magnification. (b) Cells were cultured for 24 hours with different dosages of pilocarpine or timolol, or EGF+BPE or vehicle. Pooled data from three experiments are shown. ** indicates p

    Journal: Cornea

    Article Title: Influence of pilocarpine and timolol on human meibomian gland epithelial cells

    doi: 10.1097/ICO.0000000000001181

    Figure Lengend Snippet: Influence of clinical doses of pilocarpine or timolol on IHMGEC survival. IHMGECs (n = 3 wells/treatment/experiment) were treated with various concentrations of anti-glaucoma drugs, or EGF+BPE or vehicle for 24 hours. (a) Pilocarpine and timolol induce time- and dose-dependent morphological changes in IHMGECs. Images shown are representative of three separate experiments. All images are 200X magnification. (b) Cells were cultured for 24 hours with different dosages of pilocarpine or timolol, or EGF+BPE or vehicle. Pooled data from three experiments are shown. ** indicates p

    Article Snippet: Immortalized (I) HMGECs were cultured in keratinocyte serum-free medium (KSFM), supplemented with 5 ng/ml epidermal growth factor (EGF) and 50 μg/ml bovine pituitary extract (BPE) (Life Technologies, Grand Island, NY).

    Techniques: Cell Culture