Journal: Investigative Ophthalmology & Visual Science
Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells
Figure Lengend Snippet: Effect of EGF + BPE, and serum on lipid accumulation in human meibomian gland and conjunctival epithelial cells. Cells were cultured in SFM or in media supplemented with EGF + BPE or serum. Cells were fixed and stained with LipidTox Green neutral lipid
Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.
Techniques: Cell Culture, Staining