Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Effect of EGF + BPE, serum, and media on the proliferation of human meibomian gland epithelial cells. Cells ( n = 3 wells/condition) at passages 44 ( A ) and 49 ( B ) were cultured as described in the text. Values equal the mean ± SE. *Significantly

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Cell Culture

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Effect of EGF + BPE, and serum on lipid accumulation in human meibomian gland and conjunctival epithelial cells. Cells were cultured in SFM or in media supplemented with EGF + BPE or serum. Cells were fixed and stained with LipidTox Green neutral lipid

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Cell Culture, Staining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Impact of EGF, BPE, and EGF + BPE on the proliferation of human meibomian gland epithelial cells. Cells ( n = 3 wells/condition) at passages 50 ( A ) and 16 ( B ) were cultured as described in the text. Values equal the mean ± SE. Significantly (*

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Cell Culture

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF and BPE on Gene Expression in Human Meibomian Gland Epithelial Cells

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Effect of Growth Factors on the Proliferation and Gene Expression of Human Meibomian Gland Epithelial Cells

doi: 10.1167/iovs.12-11221

Figure Lengend Snippet: Influence of EGF, BPE, and EGF + BPE on the proliferation of human meibomian gland epithelial cells. Cells at passage 50 were cultured as explained in the text. Values represent the mean ± SE. *Significantly ( P

Article Snippet: Immortalized human meibomian gland epithelial cells, recently generated in our laboratory, were cultured in 6-well plates (BD Falcon, San Jose, CA) or 10 cm culture dishes (Corning Incorporated, Corning, NY) at 37°C in 5% CO2 in one of the following media: MCDB 153 medium (MCDB; Sigma-Aldrich, St. Louis, MO), which was developed originally for the serum-free growth of specific cell types; serum-free keratinocyte basal medium (SFM; Invitrogen-Gibco, Grand Island, NY); SFM with 5 ng/mL EGF (Invitrogen-Gibco); SFM with 50 μg/mL BPE (Invitrogen-Gibco); SFM with EGF + BPE; or serum-containing medium, which consisted of an equal volume of Dulbecco's modified Eagle's medium (DMEM) and Ham's F12 (Invitrogen-Gibco), supplemented with 10% fetal bovine serum (Invitrogen-Gibco) and 10 ng/mL EGF.

Techniques: Cell Culture

Journal: Cornea

Article Title: Influence of pilocarpine and timolol on human meibomian gland epithelial cells

doi: 10.1097/ICO.0000000000001181

Figure Lengend Snippet: Effect of physiologic doses of pilocarpine or timolol on IHMGEC survival and proliferation. (a,b) IHMGECs (n = 3 wells/treatment/experiment) were treated with 0.001% pilocarpine, 0.0002% timolol, or vehicle for 24 hours. A representative of three separate experiments is shown. (c,d) Cells were cultured with 0.001% pilocarpine or 0.0002% timolol for 5 days in KSFM with or without EGF+BPE. A representative example of three experiments is shown. ** indicates p

Article Snippet: Immortalized (I) HMGECs were cultured in keratinocyte serum-free medium (KSFM), supplemented with 5 ng/ml epidermal growth factor (EGF) and 50 μg/ml bovine pituitary extract (BPE) (Life Technologies, Grand Island, NY).

Techniques: Cell Culture

Journal: Cornea

Article Title: Influence of pilocarpine and timolol on human meibomian gland epithelial cells

doi: 10.1097/ICO.0000000000001181

Figure Lengend Snippet: Influence of clinical doses of pilocarpine or timolol on IHMGEC survival. IHMGECs (n = 3 wells/treatment/experiment) were treated with various concentrations of anti-glaucoma drugs, or EGF+BPE or vehicle for 24 hours. (a) Pilocarpine and timolol induce time- and dose-dependent morphological changes in IHMGECs. Images shown are representative of three separate experiments. All images are 200X magnification. (b) Cells were cultured for 24 hours with different dosages of pilocarpine or timolol, or EGF+BPE or vehicle. Pooled data from three experiments are shown. ** indicates p

Article Snippet: Immortalized (I) HMGECs were cultured in keratinocyte serum-free medium (KSFM), supplemented with 5 ng/ml epidermal growth factor (EGF) and 50 μg/ml bovine pituitary extract (BPE) (Life Technologies, Grand Island, NY).

Techniques: Cell Culture