Article Title: Analysis of circulating protein aggregates reveals pathological hallmarks of amyotrophic lateral sclerosis
Figure Lengend Snippet: Proteins identified by LC-MS/MS in circulating protein aggregates (CPA) enriched from ALS and HC pooled plasma samples and from aggregates enriched from brain. (A) Venn diagram showing CPA proteins unique to or shared by ALS and HC. (B) Venn diagram showing HC and ALS CPA proteins shared by brain aggregates. Five proteins were expressed in all 3 aggregate groups (actin cytoplasmic 1, tubulin alpha-4A chain isoform 2, clathrin heavy chain 1 isoform 2, collagen alpha-1(VI) and plectin isoform 7), while brain aggregates shared only one protein with ALS and HC CPA (cytoplasmic dynein 1 heavy chain 1 and collagen alpha-2(VI), respectively).
Article Snippet: Circulating and brain protein aggregates protease digestion Aggregates-enriched fractions were enzymatically digested using trypsin (V542A, Promega), α-Chymotrypsin (referred as Chymotrypsin in the text, C4129, Sigma), Calpain (208712, Millipore) and Enterokinase (11334115001, Roche).
Techniques: Liquid Chromatography with Mass Spectroscopy