bone mesenchymal stromal cells Search Results


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  • 95
    ATCC human umbilical cord matrix wharton s jelly mesenchymal stem cells wj mscs
    Human Umbilical Cord Matrix Wharton S Jelly Mesenchymal Stem Cells Wj Mscs, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore mesenchymal stromal cells msc
    Mesenchymal Stromal Cells Msc, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cyagen Biosciences cell culture human bone mesenchymal stromal cells bmscs
    Cell Culture Human Bone Mesenchymal Stromal Cells Bmscs, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Lonza human bone marrow mesenchymal stem cells
    Growth rates and metabolic indices of <t>mesenchymal</t> <t>stem</t> <t>cells</t> (MSCs) and <t>human</t> umbilical vein endothelial cells (HUVECs) under various medium compositions. Growth rates of MSCs and HUVECs ( n =5) under various medium compositions ranging from 100% complete
    Human Bone Marrow Mesenchymal Stem Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Lonza bone marrow mesenchymal stromal cells
    Growth rates and metabolic indices of <t>mesenchymal</t> <t>stem</t> <t>cells</t> (MSCs) and <t>human</t> umbilical vein endothelial cells (HUVECs) under various medium compositions. Growth rates of MSCs and HUVECs ( n =5) under various medium compositions ranging from 100% complete
    Bone Marrow Mesenchymal Stromal Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Lonza bone marrow stromal cells
    <t>Stromal</t> <t>cells</t> cultured in BMoaC express proteins found in native <t>marrow.</t> ( A ) Osteopontin (green) is expressed in the ( i ) endosteal and ( ii ) perivascular niches. EC (red) counterstained with CD34. ( B ) SDF-1 is detected adjacent to EC (red) in the ( i ) endosteal niche with more stromal cells appearing to express SDF-1 in the ( ii ) perivascular niche. ( C ) EC (red) are adjacent to stromal cells that express leptin as seen in the (i) endosteal and (ii) perivascular niche. Nuclei counterstained with DAPI (blue) (D) Marker expression of hOB and BMSC as determined by flow cytometry, n=3.
    Bone Marrow Stromal Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Lonza mesenchymal stromal cells hmscs
    <t>Stromal</t> <t>cells</t> cultured in BMoaC express proteins found in native <t>marrow.</t> ( A ) Osteopontin (green) is expressed in the ( i ) endosteal and ( ii ) perivascular niches. EC (red) counterstained with CD34. ( B ) SDF-1 is detected adjacent to EC (red) in the ( i ) endosteal niche with more stromal cells appearing to express SDF-1 in the ( ii ) perivascular niche. ( C ) EC (red) are adjacent to stromal cells that express leptin as seen in the (i) endosteal and (ii) perivascular niche. Nuclei counterstained with DAPI (blue) (D) Marker expression of hOB and BMSC as determined by flow cytometry, n=3.
    Mesenchymal Stromal Cells Hmscs, supplied by Lonza, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Exosome Diagnostics mesenchymal stromal cells derived exosome
    Overview of proposed mechanisms of MSC <t>exosome-mediated</t> recovery in EAE. Upon injection, exosomes could bypass the blood spinal cord barrier either through gaps between endothelial cells (paracellular route) or by first being endocytosed and then exocytosed by endothelial cells (transcellular route). Possessing a complex mixture of RNA and proteins targeting different pathways enable exosomes to exert their therapeutic efficacy probably by downregulating inflammation and inducing tolerance by increasing the number of Tregs. Over time, resolving inflammation in spinal cord is associated with decreased demyelination, resulting in recovery.
    Mesenchymal Stromal Cells Derived Exosome, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    PromoCell human bone marrow mesenchymal stromal cells bm mscs
    Urea and albumin concentration of <t>HepG2</t> and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, <t>HepG2—BM-MSCs,</t> and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.
    Human Bone Marrow Mesenchymal Stromal Cells Bm Mscs, supplied by PromoCell, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Lonza human bone marrow derived mesenchymal stem cells msc
    Urea and albumin concentration of <t>HepG2</t> and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, <t>HepG2—BM-MSCs,</t> and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.
    Human Bone Marrow Derived Mesenchymal Stem Cells Msc, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    ScienCell rat bone marrow derived mesenchymal stromal cells
    Urea and albumin concentration of <t>HepG2</t> and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, <t>HepG2—BM-MSCs,</t> and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.
    Rat Bone Marrow Derived Mesenchymal Stromal Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    ScienCell human mesenchymal stromal cells mscs
    Urea and albumin concentration of <t>HepG2</t> and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, <t>HepG2—BM-MSCs,</t> and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.
    Human Mesenchymal Stromal Cells Mscs, supplied by ScienCell, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Lonza bone marrow mesenchymal stromal cells bmscs
    Urea and albumin concentration of <t>HepG2</t> and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, <t>HepG2—BM-MSCs,</t> and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.
    Bone Marrow Mesenchymal Stromal Cells Bmscs, supplied by Lonza, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    St. Jude Children's Research Hospital bone marrow bm mesenchymal stromal cells
    Urea and albumin concentration of <t>HepG2</t> and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, <t>HepG2—BM-MSCs,</t> and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.
    Bone Marrow Bm Mesenchymal Stromal Cells, supplied by St. Jude Children's Research Hospital, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Microtissues bone marrow derived mesenchymal stromal cells
    <t>Adipose-derived</t> <t>mesenchymal</t> <t>stromal</t> <t>cells</t> (adMSC) Cotransplantation drives HMEC vessel-like structure formation surrounding modules. Photographs of human microvascular endothelial cells (HMEC)+adMSC modular implants appear red, with blood vessels visible
    Bone Marrow Derived Mesenchymal Stromal Cells, supplied by Microtissues, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Lonza mesenchymal stem cells mscs
    Reaggregation of dispersed purified human β-cells. ( a ) Pure β-cells were labeled with GFP and traced in 3 different culture conditions: in monolayer (“single β”), β-cell-only aggregation (“β”), or β-cell aggregation with stromal cells including <t>HUVECs</t> and <t>MSCs</t> (“β+HM”). Live imaging at indicated days (middle panels) and immunofluorescence at day 7 (right panels) show β-cell-only pseudoislets were self-organized by Day 5, whereas β+HM aggregates were constituted in only 1 day. β-cells in β+HM pseudoislets located at the periphery, while HM cells formed the core of the aggregates (red and blue, respectively). ( b ) To determine the optimal number of β-cells per pseudoislet, GFP + transduced β-cells were seeded on aggregation-plate-wells at the indicated densities. 7 days after culture, aggregates were harvested and analyzed. Aggregates were uniform in size. Pseudoislet size correlated with the number of cells seeded per well. It was reported that human islet cell aggregates with a diameter 100—150 μm, consisting of 1000 cells, show a comparable function to native islets 24 ; we thus decided to perform reaggregation experiments at 1000 β-cells/pseudoislet (1000 β-cells, 129.6±3.1 μm). β-cell aggregates with HM were also analyzed. n = 8 pseudoislets for 500 β-cells, n = 8 pseudoislets for 1000 β-cells, n = 9 pseudoislets for 2000 β-cells, n = 8 pseudoislets for 3000 β-cells, and n = 52 pseudoislets for 1000 β-cells + 400 HUVECs + 100 MSCs. ( c ) Immunofluorescence at indicated time-points in β-cell pseudoislets and β-cell+HM pseudoislets. ( d ) TUNEL staining (green) showed rare apoptotic cells (0.8%) in β-cell aggregates after 7 day-culture. ( e ) qPCR analyses of INSULIN and PDX1 expression in monolayer and aggregated β-cells showing higher expression of β-cell markers in reaggregated β-cells. Data are expressed as fold-change relative to the value in single β-cells. * p = 0.022 in qPCR for INS , * p = 0.026 in qPCR for PDX1 , Mann-Whitney test, two-tailed. n = 6 donor samples. ( f ) ELISA measurements of static glucose-stimulated human insulin release at 3 mM (Low) and 20 mM (Hi) glucose showing glucose-responsive C-peptide secretion in both β and β+HM aggregates, but not in single β-cells. **** p
    Mesenchymal Stem Cells Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    BioBridge mesenchymal stromal cells mscs
    Reaggregation of dispersed purified human β-cells. ( a ) Pure β-cells were labeled with GFP and traced in 3 different culture conditions: in monolayer (“single β”), β-cell-only aggregation (“β”), or β-cell aggregation with stromal cells including <t>HUVECs</t> and <t>MSCs</t> (“β+HM”). Live imaging at indicated days (middle panels) and immunofluorescence at day 7 (right panels) show β-cell-only pseudoislets were self-organized by Day 5, whereas β+HM aggregates were constituted in only 1 day. β-cells in β+HM pseudoislets located at the periphery, while HM cells formed the core of the aggregates (red and blue, respectively). ( b ) To determine the optimal number of β-cells per pseudoislet, GFP + transduced β-cells were seeded on aggregation-plate-wells at the indicated densities. 7 days after culture, aggregates were harvested and analyzed. Aggregates were uniform in size. Pseudoislet size correlated with the number of cells seeded per well. It was reported that human islet cell aggregates with a diameter 100—150 μm, consisting of 1000 cells, show a comparable function to native islets 24 ; we thus decided to perform reaggregation experiments at 1000 β-cells/pseudoislet (1000 β-cells, 129.6±3.1 μm). β-cell aggregates with HM were also analyzed. n = 8 pseudoislets for 500 β-cells, n = 8 pseudoislets for 1000 β-cells, n = 9 pseudoislets for 2000 β-cells, n = 8 pseudoislets for 3000 β-cells, and n = 52 pseudoislets for 1000 β-cells + 400 HUVECs + 100 MSCs. ( c ) Immunofluorescence at indicated time-points in β-cell pseudoislets and β-cell+HM pseudoislets. ( d ) TUNEL staining (green) showed rare apoptotic cells (0.8%) in β-cell aggregates after 7 day-culture. ( e ) qPCR analyses of INSULIN and PDX1 expression in monolayer and aggregated β-cells showing higher expression of β-cell markers in reaggregated β-cells. Data are expressed as fold-change relative to the value in single β-cells. * p = 0.022 in qPCR for INS , * p = 0.026 in qPCR for PDX1 , Mann-Whitney test, two-tailed. n = 6 donor samples. ( f ) ELISA measurements of static glucose-stimulated human insulin release at 3 mM (Low) and 20 mM (Hi) glucose showing glucose-responsive C-peptide secretion in both β and β+HM aggregates, but not in single β-cells. **** p
    Mesenchymal Stromal Cells Mscs, supplied by BioBridge, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Growth rates and metabolic indices of mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs) under various medium compositions. Growth rates of MSCs and HUVECs ( n =5) under various medium compositions ranging from 100% complete

    Journal: Tissue Engineering. Part A

    Article Title: A Mathematical Model Predicting the Coculture Dynamics of Endothelial and Mesenchymal Stem Cells for Tissue Regeneration

    doi: 10.1089/ten.tea.2012.0507

    Figure Lengend Snippet: Growth rates and metabolic indices of mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs) under various medium compositions. Growth rates of MSCs and HUVECs ( n =5) under various medium compositions ranging from 100% complete

    Article Snippet: Human bone marrow mesenchymal stem cells and HUVECs (Lonza™, Basel, Switzerland) were cultured using two types of media and their combinations: complete Lonza endothelial growth medium-2 (EGM-2)™, HUVEC-designated medium according to the literature , ; and complete αMEM (minimum essential medium Eagle, alpha modification; Sigma-Aldrich™, St. Louis, MO), MSC-designated medium according to the literature, – supplemented with 10% Gibco® fetal bovine serum (FBS), 1% L-glutamine, 5 ng/mL basic fibroblast growth factor, and 1% antibiotic-antimycotic (Invitrogen™, Carlsbad, CA).

    Techniques:

    Flow cytometric analysis of PDGFR α + cells. ( a ) Human muscle-derived cells were analyzed for PDGFR α and CD56 expression. Representative data of 30 independent experiments are shown. ( b ) Positive gates were set by analyzing negative control samples stained with isotype control antibody or secondary reagent only. ( c ) Sorted PDGFR α + cells were reanalyzed for PDGFR α and CD56 expression. Consistent results were obtained from two independent experiments. ( d ) Sorted CD56 + cells were reanalyzed for PDGFR α and CD56 expression. Consistent results were obtained from two independent experiments. Expressions of indicated cell surface antigens, PDGFR α + cells ( e ), CD56 + cells ( f ), and bone marrow-derived mesenchymal stem cells (BMMSC, g ) were analyzed. Positive gates were set by analyzing negative control samples stained with isotype control antibody (dotted line). The percentages of each cell population are shown in the panels

    Journal: Cell Death & Disease

    Article Title: Identification and characterization of PDGFRα+ mesenchymal progenitors in human skeletal muscle

    doi: 10.1038/cddis.2014.161

    Figure Lengend Snippet: Flow cytometric analysis of PDGFR α + cells. ( a ) Human muscle-derived cells were analyzed for PDGFR α and CD56 expression. Representative data of 30 independent experiments are shown. ( b ) Positive gates were set by analyzing negative control samples stained with isotype control antibody or secondary reagent only. ( c ) Sorted PDGFR α + cells were reanalyzed for PDGFR α and CD56 expression. Consistent results were obtained from two independent experiments. ( d ) Sorted CD56 + cells were reanalyzed for PDGFR α and CD56 expression. Consistent results were obtained from two independent experiments. Expressions of indicated cell surface antigens, PDGFR α + cells ( e ), CD56 + cells ( f ), and bone marrow-derived mesenchymal stem cells (BMMSC, g ) were analyzed. Positive gates were set by analyzing negative control samples stained with isotype control antibody (dotted line). The percentages of each cell population are shown in the panels

    Article Snippet: Human bone marrow mesenchymal stromal cells (Lonza, Basel, Switzerland) were maintained in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin at 37 °C in 5% CO2 and 21% O2 .

    Techniques: Flow Cytometry, Derivative Assay, Expressing, Negative Control, Staining

    Stromal cells cultured in BMoaC express proteins found in native marrow. ( A ) Osteopontin (green) is expressed in the ( i ) endosteal and ( ii ) perivascular niches. EC (red) counterstained with CD34. ( B ) SDF-1 is detected adjacent to EC (red) in the ( i ) endosteal niche with more stromal cells appearing to express SDF-1 in the ( ii ) perivascular niche. ( C ) EC (red) are adjacent to stromal cells that express leptin as seen in the (i) endosteal and (ii) perivascular niche. Nuclei counterstained with DAPI (blue) (D) Marker expression of hOB and BMSC as determined by flow cytometry, n=3.

    Journal: bioRxiv

    Article Title: Organ-on-a-chip model of vascularized human bone marrow niches

    doi: 10.1101/2020.04.17.039339

    Figure Lengend Snippet: Stromal cells cultured in BMoaC express proteins found in native marrow. ( A ) Osteopontin (green) is expressed in the ( i ) endosteal and ( ii ) perivascular niches. EC (red) counterstained with CD34. ( B ) SDF-1 is detected adjacent to EC (red) in the ( i ) endosteal niche with more stromal cells appearing to express SDF-1 in the ( ii ) perivascular niche. ( C ) EC (red) are adjacent to stromal cells that express leptin as seen in the (i) endosteal and (ii) perivascular niche. Nuclei counterstained with DAPI (blue) (D) Marker expression of hOB and BMSC as determined by flow cytometry, n=3.

    Article Snippet: Bone marrow stromal cells (BMSC, Lonza) were cultured in Myelocult 5100 (StemCell Technologies) supplemented with 1 μM hydrocortisone (StemCell Technologies), 2 mM L-glutamine (ThermoFisher), and 50 U/mL Penicillin-Streptomycin (ThermoFisher).

    Techniques: Cell Culture, Marker, Expressing, Flow Cytometry

    Overview of proposed mechanisms of MSC exosome-mediated recovery in EAE. Upon injection, exosomes could bypass the blood spinal cord barrier either through gaps between endothelial cells (paracellular route) or by first being endocytosed and then exocytosed by endothelial cells (transcellular route). Possessing a complex mixture of RNA and proteins targeting different pathways enable exosomes to exert their therapeutic efficacy probably by downregulating inflammation and inducing tolerance by increasing the number of Tregs. Over time, resolving inflammation in spinal cord is associated with decreased demyelination, resulting in recovery.

    Journal: ACS nano

    Article Title: Stem Cell-Derived Exosomes as Nanotherapeutics for Autoimmune and Neurodegenerative Disorders

    doi: 10.1021/acsnano.9b01004

    Figure Lengend Snippet: Overview of proposed mechanisms of MSC exosome-mediated recovery in EAE. Upon injection, exosomes could bypass the blood spinal cord barrier either through gaps between endothelial cells (paracellular route) or by first being endocytosed and then exocytosed by endothelial cells (transcellular route). Possessing a complex mixture of RNA and proteins targeting different pathways enable exosomes to exert their therapeutic efficacy probably by downregulating inflammation and inducing tolerance by increasing the number of Tregs. Over time, resolving inflammation in spinal cord is associated with decreased demyelination, resulting in recovery.

    Article Snippet: [ ] [ ] [ ] (67) Chen W; Huang Y; Han J; Yu L; Li Y; Lu Z; Li H; Liu Z; Shi C; Duan F; Xiao Y Immunomodulatory Effects of Mesenchymal Stromal Cells-Derived Exosome .

    Techniques: Injection

    MSC-derived exosome-treated mice showed a reduced number of infiltrated macrophages/microglia and T cells in the spinal cords of EAE mice. (A) Immunohistochemistry of Iba-1, a Ca 2+ -binding protein indicative of macrophages and microglia, and the nuclear stain DAPI in spinal cord sections of EAE mice (Left and middle panel scale: 400 μ m; right panel scale: 80 μ m). Yellow inset frames are magnified images showing the morphology of macrophages. (B) Quantification of Iba-1 positive cells from immunohistochemistry images. Iba-1 positive cells were counted in the spinal cords’ sections in both white and gray matter areas ( n = 3; ** p

    Journal: ACS nano

    Article Title: Stem Cell-Derived Exosomes as Nanotherapeutics for Autoimmune and Neurodegenerative Disorders

    doi: 10.1021/acsnano.9b01004

    Figure Lengend Snippet: MSC-derived exosome-treated mice showed a reduced number of infiltrated macrophages/microglia and T cells in the spinal cords of EAE mice. (A) Immunohistochemistry of Iba-1, a Ca 2+ -binding protein indicative of macrophages and microglia, and the nuclear stain DAPI in spinal cord sections of EAE mice (Left and middle panel scale: 400 μ m; right panel scale: 80 μ m). Yellow inset frames are magnified images showing the morphology of macrophages. (B) Quantification of Iba-1 positive cells from immunohistochemistry images. Iba-1 positive cells were counted in the spinal cords’ sections in both white and gray matter areas ( n = 3; ** p

    Article Snippet: [ ] [ ] [ ] (67) Chen W; Huang Y; Han J; Yu L; Li Y; Lu Z; Li H; Liu Z; Shi C; Duan F; Xiao Y Immunomodulatory Effects of Mesenchymal Stromal Cells-Derived Exosome .

    Techniques: Derivative Assay, Mouse Assay, Immunohistochemistry, Binding Assay, Staining

    Urea and albumin concentration of HepG2 and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, HepG2—BM-MSCs, and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.

    Journal: PLoS ONE

    Article Title: The potential of cell sheet technique on the development of hepatocellular carcinoma in rat models

    doi: 10.1371/journal.pone.0184004

    Figure Lengend Snippet: Urea and albumin concentration of HepG2 and stromal cell sheets at day 1 and day 7. The cells were co-cultivated in up-cell dish to form a cell sheet. Where the conditioned medium was collected and HepG2 only cell sheet as control, whereas each stromal cells bar represents co-culture of HepG2 and stromal cells. Albumin or urea secretion by HepG2—UCMSCs, HepG2—BM-MSCs, and HepG2 alone significantly increased at day 7 in culture as compared to day 1, P = 0.009 and P = 0.0004 respectively. Mean±SD. Two-way ANOVA.

    Article Snippet: Cell culture Cell lines of liver hepatocellular carcinoma (HepG2, PromoCell, USA), human bone marrow mesenchymal stromal cells (BM-MSCs) (PromoCell, USA), human umbilical cord mesenchymal stromal cells (UC-MSCs) (PromoCell, USA) were cultured in a culture medium consists of high glucose DMEM (Sigma, Tokyo, Japan), 10% fetal bovine serum (FBS) (CCB, NICHIREI BIOSCIENCE, Tokyo, Japan) and 1% penicillin/streptomycin (Life Technology, Carlsbad, CA, USA) while human umbilical vein endothelial cells (HUVECs) (Lonza, USA) were cultured in endothelial cell culture medium (EBM) (EGM, Lonza, Tokyo, Japan) containing 2% FBS and vascular endothelial growth factor (VEGF).

    Techniques: Concentration Assay, Co-Culture Assay

    Fabrication of cell sheets using UPcell dishes. (A): (a); HepG2, (b); co-culture of HepG2+BM-MSCs, (c); HepG2+UC-MSC. The Mean of the total surface areas of HepG2, HepG2+human UC-SCs and HepG2+human BM-MSCs; 20 mm 2, 10 mm 2, and 19.8 mm 2 respectively. (B); Fluorescent staining of F-actin filaments in fixed cell sheet of HepG2 and stromal cells. Where (a); HepG2 and UC-SC, and (b) HepG2 and BM-SC. x100 magnification. Staining of F- actin (red) indicates the denseness of differentiated tube formation network of HepG2 and UC-MSC in pouch-like cell sheets in vitro.

    Journal: PLoS ONE

    Article Title: The potential of cell sheet technique on the development of hepatocellular carcinoma in rat models

    doi: 10.1371/journal.pone.0184004

    Figure Lengend Snippet: Fabrication of cell sheets using UPcell dishes. (A): (a); HepG2, (b); co-culture of HepG2+BM-MSCs, (c); HepG2+UC-MSC. The Mean of the total surface areas of HepG2, HepG2+human UC-SCs and HepG2+human BM-MSCs; 20 mm 2, 10 mm 2, and 19.8 mm 2 respectively. (B); Fluorescent staining of F-actin filaments in fixed cell sheet of HepG2 and stromal cells. Where (a); HepG2 and UC-SC, and (b) HepG2 and BM-SC. x100 magnification. Staining of F- actin (red) indicates the denseness of differentiated tube formation network of HepG2 and UC-MSC in pouch-like cell sheets in vitro.

    Article Snippet: Cell culture Cell lines of liver hepatocellular carcinoma (HepG2, PromoCell, USA), human bone marrow mesenchymal stromal cells (BM-MSCs) (PromoCell, USA), human umbilical cord mesenchymal stromal cells (UC-MSCs) (PromoCell, USA) were cultured in a culture medium consists of high glucose DMEM (Sigma, Tokyo, Japan), 10% fetal bovine serum (FBS) (CCB, NICHIREI BIOSCIENCE, Tokyo, Japan) and 1% penicillin/streptomycin (Life Technology, Carlsbad, CA, USA) while human umbilical vein endothelial cells (HUVECs) (Lonza, USA) were cultured in endothelial cell culture medium (EBM) (EGM, Lonza, Tokyo, Japan) containing 2% FBS and vascular endothelial growth factor (VEGF).

    Techniques: Co-Culture Assay, Staining, In Vitro

    Human VEGF concentration of co-cultured HepG2 with MSCs (UC or BM) after one week of incubation. HUVECs were used as a positive control. Tow-way NOVA (P = 0.001).

    Journal: PLoS ONE

    Article Title: The potential of cell sheet technique on the development of hepatocellular carcinoma in rat models

    doi: 10.1371/journal.pone.0184004

    Figure Lengend Snippet: Human VEGF concentration of co-cultured HepG2 with MSCs (UC or BM) after one week of incubation. HUVECs were used as a positive control. Tow-way NOVA (P = 0.001).

    Article Snippet: Cell culture Cell lines of liver hepatocellular carcinoma (HepG2, PromoCell, USA), human bone marrow mesenchymal stromal cells (BM-MSCs) (PromoCell, USA), human umbilical cord mesenchymal stromal cells (UC-MSCs) (PromoCell, USA) were cultured in a culture medium consists of high glucose DMEM (Sigma, Tokyo, Japan), 10% fetal bovine serum (FBS) (CCB, NICHIREI BIOSCIENCE, Tokyo, Japan) and 1% penicillin/streptomycin (Life Technology, Carlsbad, CA, USA) while human umbilical vein endothelial cells (HUVECs) (Lonza, USA) were cultured in endothelial cell culture medium (EBM) (EGM, Lonza, Tokyo, Japan) containing 2% FBS and vascular endothelial growth factor (VEGF).

    Techniques: Concentration Assay, Cell Culture, Incubation, Positive Control

    Vascularisation of co-culture of HUVEC or MSCs with HepG2 on Matrigel after 14h incubation. (A) HUVEC+HepG2, (B) UC-MSC+HepG2, (C D) BM-MSC+HepG2. Cells were seeded into Matrigel in the presence of VEGF. Tubes started to form after 14 hours. CD31 (green) and Hoechst 33258 staining (blue). The presented ratio of the co-culture cells is 1:1. 4x magnification.

    Journal: PLoS ONE

    Article Title: The potential of cell sheet technique on the development of hepatocellular carcinoma in rat models

    doi: 10.1371/journal.pone.0184004

    Figure Lengend Snippet: Vascularisation of co-culture of HUVEC or MSCs with HepG2 on Matrigel after 14h incubation. (A) HUVEC+HepG2, (B) UC-MSC+HepG2, (C D) BM-MSC+HepG2. Cells were seeded into Matrigel in the presence of VEGF. Tubes started to form after 14 hours. CD31 (green) and Hoechst 33258 staining (blue). The presented ratio of the co-culture cells is 1:1. 4x magnification.

    Article Snippet: Cell culture Cell lines of liver hepatocellular carcinoma (HepG2, PromoCell, USA), human bone marrow mesenchymal stromal cells (BM-MSCs) (PromoCell, USA), human umbilical cord mesenchymal stromal cells (UC-MSCs) (PromoCell, USA) were cultured in a culture medium consists of high glucose DMEM (Sigma, Tokyo, Japan), 10% fetal bovine serum (FBS) (CCB, NICHIREI BIOSCIENCE, Tokyo, Japan) and 1% penicillin/streptomycin (Life Technology, Carlsbad, CA, USA) while human umbilical vein endothelial cells (HUVECs) (Lonza, USA) were cultured in endothelial cell culture medium (EBM) (EGM, Lonza, Tokyo, Japan) containing 2% FBS and vascular endothelial growth factor (VEGF).

    Techniques: Co-Culture Assay, Incubation, Staining

    Vascularisation of HepG2 and MSCs on Matrigel after 14h incubation. (A) HepG2, (B)HUVECs, (C) BM-MSC, (D) UC-MSC. Cells were seeded into Matrigel in the presence of VEGF. CD31 (green) and Hoechst 33258 staining (blue). x4 magnification.

    Journal: PLoS ONE

    Article Title: The potential of cell sheet technique on the development of hepatocellular carcinoma in rat models

    doi: 10.1371/journal.pone.0184004

    Figure Lengend Snippet: Vascularisation of HepG2 and MSCs on Matrigel after 14h incubation. (A) HepG2, (B)HUVECs, (C) BM-MSC, (D) UC-MSC. Cells were seeded into Matrigel in the presence of VEGF. CD31 (green) and Hoechst 33258 staining (blue). x4 magnification.

    Article Snippet: Cell culture Cell lines of liver hepatocellular carcinoma (HepG2, PromoCell, USA), human bone marrow mesenchymal stromal cells (BM-MSCs) (PromoCell, USA), human umbilical cord mesenchymal stromal cells (UC-MSCs) (PromoCell, USA) were cultured in a culture medium consists of high glucose DMEM (Sigma, Tokyo, Japan), 10% fetal bovine serum (FBS) (CCB, NICHIREI BIOSCIENCE, Tokyo, Japan) and 1% penicillin/streptomycin (Life Technology, Carlsbad, CA, USA) while human umbilical vein endothelial cells (HUVECs) (Lonza, USA) were cultured in endothelial cell culture medium (EBM) (EGM, Lonza, Tokyo, Japan) containing 2% FBS and vascular endothelial growth factor (VEGF).

    Techniques: Incubation, Staining

    Appearance of rat’s liver after one month after cell sheet transplantation. Where (A) appearance of tumour generated from HepG2 and BM-SC cell sheet, and a mass (2 cm in size) underneath the skin of the rats. (B) cell sheet of HepG2 alone (C) HepG2 and UC-MSC cell sheet, and (D) attached cell sheet of HepG2 and UC-MSC on the liver without forming any tumour. The average size of the tumour developed after transplantation of only HepG2, HepG2 + BM-MSCs, and HepG2+UC-MSC are; 4.5cm, 4cm and 2.5cm respectively, 5 rats was used in each sample group.

    Journal: PLoS ONE

    Article Title: The potential of cell sheet technique on the development of hepatocellular carcinoma in rat models

    doi: 10.1371/journal.pone.0184004

    Figure Lengend Snippet: Appearance of rat’s liver after one month after cell sheet transplantation. Where (A) appearance of tumour generated from HepG2 and BM-SC cell sheet, and a mass (2 cm in size) underneath the skin of the rats. (B) cell sheet of HepG2 alone (C) HepG2 and UC-MSC cell sheet, and (D) attached cell sheet of HepG2 and UC-MSC on the liver without forming any tumour. The average size of the tumour developed after transplantation of only HepG2, HepG2 + BM-MSCs, and HepG2+UC-MSC are; 4.5cm, 4cm and 2.5cm respectively, 5 rats was used in each sample group.

    Article Snippet: Cell culture Cell lines of liver hepatocellular carcinoma (HepG2, PromoCell, USA), human bone marrow mesenchymal stromal cells (BM-MSCs) (PromoCell, USA), human umbilical cord mesenchymal stromal cells (UC-MSCs) (PromoCell, USA) were cultured in a culture medium consists of high glucose DMEM (Sigma, Tokyo, Japan), 10% fetal bovine serum (FBS) (CCB, NICHIREI BIOSCIENCE, Tokyo, Japan) and 1% penicillin/streptomycin (Life Technology, Carlsbad, CA, USA) while human umbilical vein endothelial cells (HUVECs) (Lonza, USA) were cultured in endothelial cell culture medium (EBM) (EGM, Lonza, Tokyo, Japan) containing 2% FBS and vascular endothelial growth factor (VEGF).

    Techniques: Transplantation Assay, Generated

    Adipose-derived mesenchymal stromal cells (adMSC) Cotransplantation drives HMEC vessel-like structure formation surrounding modules. Photographs of human microvascular endothelial cells (HMEC)+adMSC modular implants appear red, with blood vessels visible

    Journal: Tissue Engineering. Part A

    Article Title: Cotransplantation of Adipose-Derived Mesenchymal Stromal Cells and Endothelial Cells in a Modular Construct Drives Vascularization in SCID/bg Mice

    doi: 10.1089/ten.tea.2011.0467

    Figure Lengend Snippet: Adipose-derived mesenchymal stromal cells (adMSC) Cotransplantation drives HMEC vessel-like structure formation surrounding modules. Photographs of human microvascular endothelial cells (HMEC)+adMSC modular implants appear red, with blood vessels visible

    Article Snippet: Bone marrow-derived mesenchymal stromal cells enhance chimeric vessel development driven by endothelial cell coated microtissues.

    Techniques: Derivative Assay

    Reaggregation of dispersed purified human β-cells. ( a ) Pure β-cells were labeled with GFP and traced in 3 different culture conditions: in monolayer (“single β”), β-cell-only aggregation (“β”), or β-cell aggregation with stromal cells including HUVECs and MSCs (“β+HM”). Live imaging at indicated days (middle panels) and immunofluorescence at day 7 (right panels) show β-cell-only pseudoislets were self-organized by Day 5, whereas β+HM aggregates were constituted in only 1 day. β-cells in β+HM pseudoislets located at the periphery, while HM cells formed the core of the aggregates (red and blue, respectively). ( b ) To determine the optimal number of β-cells per pseudoislet, GFP + transduced β-cells were seeded on aggregation-plate-wells at the indicated densities. 7 days after culture, aggregates were harvested and analyzed. Aggregates were uniform in size. Pseudoislet size correlated with the number of cells seeded per well. It was reported that human islet cell aggregates with a diameter 100—150 μm, consisting of 1000 cells, show a comparable function to native islets 24 ; we thus decided to perform reaggregation experiments at 1000 β-cells/pseudoislet (1000 β-cells, 129.6±3.1 μm). β-cell aggregates with HM were also analyzed. n = 8 pseudoislets for 500 β-cells, n = 8 pseudoislets for 1000 β-cells, n = 9 pseudoislets for 2000 β-cells, n = 8 pseudoislets for 3000 β-cells, and n = 52 pseudoislets for 1000 β-cells + 400 HUVECs + 100 MSCs. ( c ) Immunofluorescence at indicated time-points in β-cell pseudoislets and β-cell+HM pseudoislets. ( d ) TUNEL staining (green) showed rare apoptotic cells (0.8%) in β-cell aggregates after 7 day-culture. ( e ) qPCR analyses of INSULIN and PDX1 expression in monolayer and aggregated β-cells showing higher expression of β-cell markers in reaggregated β-cells. Data are expressed as fold-change relative to the value in single β-cells. * p = 0.022 in qPCR for INS , * p = 0.026 in qPCR for PDX1 , Mann-Whitney test, two-tailed. n = 6 donor samples. ( f ) ELISA measurements of static glucose-stimulated human insulin release at 3 mM (Low) and 20 mM (Hi) glucose showing glucose-responsive C-peptide secretion in both β and β+HM aggregates, but not in single β-cells. **** p

    Journal: Nature

    Article Title: Diabetes Relief in Mice by Glucose-Sensing Insulin-Secreting Human α-Cells

    doi: 10.1038/s41586-019-0942-8

    Figure Lengend Snippet: Reaggregation of dispersed purified human β-cells. ( a ) Pure β-cells were labeled with GFP and traced in 3 different culture conditions: in monolayer (“single β”), β-cell-only aggregation (“β”), or β-cell aggregation with stromal cells including HUVECs and MSCs (“β+HM”). Live imaging at indicated days (middle panels) and immunofluorescence at day 7 (right panels) show β-cell-only pseudoislets were self-organized by Day 5, whereas β+HM aggregates were constituted in only 1 day. β-cells in β+HM pseudoislets located at the periphery, while HM cells formed the core of the aggregates (red and blue, respectively). ( b ) To determine the optimal number of β-cells per pseudoislet, GFP + transduced β-cells were seeded on aggregation-plate-wells at the indicated densities. 7 days after culture, aggregates were harvested and analyzed. Aggregates were uniform in size. Pseudoislet size correlated with the number of cells seeded per well. It was reported that human islet cell aggregates with a diameter 100—150 μm, consisting of 1000 cells, show a comparable function to native islets 24 ; we thus decided to perform reaggregation experiments at 1000 β-cells/pseudoislet (1000 β-cells, 129.6±3.1 μm). β-cell aggregates with HM were also analyzed. n = 8 pseudoislets for 500 β-cells, n = 8 pseudoislets for 1000 β-cells, n = 9 pseudoislets for 2000 β-cells, n = 8 pseudoislets for 3000 β-cells, and n = 52 pseudoislets for 1000 β-cells + 400 HUVECs + 100 MSCs. ( c ) Immunofluorescence at indicated time-points in β-cell pseudoislets and β-cell+HM pseudoislets. ( d ) TUNEL staining (green) showed rare apoptotic cells (0.8%) in β-cell aggregates after 7 day-culture. ( e ) qPCR analyses of INSULIN and PDX1 expression in monolayer and aggregated β-cells showing higher expression of β-cell markers in reaggregated β-cells. Data are expressed as fold-change relative to the value in single β-cells. * p = 0.022 in qPCR for INS , * p = 0.026 in qPCR for PDX1 , Mann-Whitney test, two-tailed. n = 6 donor samples. ( f ) ELISA measurements of static glucose-stimulated human insulin release at 3 mM (Low) and 20 mM (Hi) glucose showing glucose-responsive C-peptide secretion in both β and β+HM aggregates, but not in single β-cells. **** p

    Article Snippet: Human umbilical vein endothelial cells (HUVECs) and human bone marrow–derived mesenchymal stem cells (MSCs) were purchased from Lonza (catalog number: C2519A, PT-2501) and cultured according to manufacturer’s instructions.

    Techniques: Purification, Labeling, Imaging, Immunofluorescence, TUNEL Assay, Staining, Real-time Polymerase Chain Reaction, Expressing, MANN-WHITNEY, Two Tailed Test, Enzyme-linked Immunosorbent Assay