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  • 92
    RayBiotech human bmp 7 elisa kit
    <t>ELISA</t> analysis of serum <t>BMP-7</t> levels in healthy subjects and patients with HCC. Healthy subjects (n=7); HCC stage I (n=3), II (n=12), IIIA (n=9) and IIIB (n=3). Tissues from healthy subjects were used as controls. **P
    Human Bmp 7 Elisa Kit, supplied by RayBiotech, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher bmp7 prodomain
    The <t>prodomain</t> of <t>BMP7</t> is not sufficient for maximal heterodimer activity. ( A ) Schematic illustration of ligands generated from native and chimeric BMP precursor proteins. ( B ) BMP RNAs were injected individually (200 pg) or together (100 pg of each RNA)
    Bmp7 Prodomain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cell Signaling Technology Inc bmp 7
    Effect of LIPUS stimulation on BMPs hASCs were treated by LIPUS at 20 and 30 mW cm −2 for 30 min daily, and the protein levels of BMP-2, BMP-6, <t>BMP-7,</t> and BMP-9 were detected using Western blot analysis. ( A ) Representative Western blot bands that displayed the protein expression of BMP-2, BMP-6, BMP-7, and BMP-9. ( B ) The protein levels of BMP-2, BMP-6, BMP-7, and BMP-9 relative to β-actin used as a loading control. Data are presented as mean ± SD ( n =3); * P
    Bmp 7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore bmp7
    Time course of Smad 1/5/8 phosphorylation following addition of <t>BMP7</t> ± BDNF
    Bmp7, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bmp 7  (Abcam)
    92
    Abcam bmp 7
    Western blot analyses depicting S100A4, TGF- β , <t>BMP-7,</t> Smad2/3, Smad1/5/8, and p16 INK4a expression in vivo after rhEPO treatment in UUO model. S100A4, TGF- β , Smad2/3, and p16 INK4a expression were significantly higher in UUO mice on days 3, 7, and 14 ( P
    Bmp 7, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Abcam bmp 7 antibodies
    Localization of BMP-2, BMP-4, and <t>BMP-7</t> antigens within defect sites with immobilized anti-BMP-2 mAb following in vivo implantation and retrieval after 8 weeks. (a) Representative CLSM images of titanium, alginate, and ACS groups with immobilized anti-BMP-2 mAb harvested from calvarial defects after 8 weeks. The immunofluorescence results revealed the capacity of the murine mAb to attract and hold BMP-2, -4, and -7 ligands. Scaffolds immobilized with nonspecific isotype mAb failed to show any positive staining. (b) Quantitative analysis of red fluorescence intensity of the images shown in (a). N = 4 for each group. * P
    Bmp 7 Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Proteintech bmp 7
    miR-4739 over-expression results in pleural fibrosis in mice. (a) Sequences of has-miR-4739 and the putative target sequence in the mouse or rat <t>BMP-7</t> mRNA (wild-type) or an engineered mutant of this sequence (mutant) for the luciferase activity assay. n = 3, *P
    Bmp 7, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems bmp7
    Systemic effects of <t>BMP7</t> are leptin-independent. A , B ) Treatment of leptin-deficient ob/ob mice with BMP7 adenovirus resulted in significant weight loss ( A ) and reduced food consumption ( B ) compared to ob/ob mice that received LacZ adenovirus (control)
    Bmp7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    R&D Systems human anti bmp7
    Downstream <t>BMP7</t> signaling in PCC cells A. PC12 cells were transfected with a BMP7-containing (BMP7) or an empty (mock) vector. Twenty-four h post transfection IF was performed using specific antibodies targeting integrin β1 (1:400) or BMP7 (1:100). Cell nuclei were counterstained with DAPI. In parallel, the expression of integrin β1 and BMP7 proteins was determined by western blotting using specific antibodies. α-Tubulin was used as loading control. B. PC12 cells were either transfected (txBMP7) with a Myc-BMP7 plasmid or treated with 100 ng/mL recombinant human BMP7 (rhBMP7). After 24 h, cells were collected and analyzed by western blotting using antibodies against P-Smad1/5/8 and Smad1. α-Tubulin was used as a loading control. C. PC12 cells were treated with rhBMP7 for 5, 15, 30, 45, or 60 min. Western blot analysis was performed using specific antibodies raised against integrin β1, AKT and P-AKT. α-Tubulin was used as loading control. D. We co-transfected PC12 cells with BMP7 and scr-siRNA or siRNA- Itgb1 and 24 h later proliferation, migration and invasion were assessed. Values for cells with knockdown of Itgb1 were normalized against the values of scr-siRNA-transfected cells arbitrarily set to 100%. The experiments were performed two times with three technical replicates with similar results. For proliferation, the average of the 2 experiments is shown. For migration/invasion, five random fields of each test at × 400 magnification were counted (average ± SD). **, P
    Human Anti Bmp7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems anti bmp7
    Known regulators of hepcidin have a modest effect on regulation of hepcidin in breast cancer spheroids (A) RT-qPCR of Hepcidin mRNA (normalized to Cyclophilin A) and (B) western blot analysis of pro-hepcidin and β-actin following siRNA knock-down of non-target control (NTC), BMP4, BMP6, and <t>BMP7</t> in MCF-7 spheroids. Untreated MCF-7 cells grown in 2D (A) or 3D (B) were used as controls. Statistical analysis and quantification was normalized to non-targeting control siRNA. (C) Western blot analysis of p-STAT3, total STAT-3 and Cyclophilin B in MCF-7 monolayer versus spheroids cultured for 3 days. (D) RT-qPCR of hepcidin mRNA (normalized to Cyclophilin A) following siRNA knock-down of NTC and STAT3 in MCF-7 spheroids. For statistical analysis samples were compared to non-targeting control. Untreated MCF-7 2D was used as a control.
    Anti Bmp7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    R&D Systems bmp7 antibody
    <t>BMP7</t> bioactivity was not affected in patient variants. The ability of the BMP7 variants to induce pSMAD 1/5 activation was assessed by (A) a luciferase bioassay and (B) analysis of endogenous pSMAD 1/5 levels, both in COV434 cells.
    Bmp7 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    R&D Systems bmp7 proteins
    Experimental workflow and numbers of differentially expressed probes (DEPs) resulting from BMP4 or <t>BMP7</t> treatment . (A) Seven breast cancer cell lines were cultured on 24-well plates, allowed to adhere for 24 h, and treated with the BMP ligand or vehicle for 30 min, 1 h, 3 h, 6 h, 12 h, and 24 h. Experiments were performed in triplicate, and collected cells were pooled. (B) The expression data from each cell line were individually filtered according to the following criteria: differential expression of at least 2-fold at a minimum of one time point. The number of DEPs per cell line is represented. (C) The expression data from individual time points of every cell line were filtered according to a 2-fold cutoff in expression change. The number of DEPs per time point is shown.
    Bmp7 Proteins, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    R&D Systems human bmp7
    Expression of <t>BMP7</t> in MCL lymphoma cells. Figure 2A: from patients tested by DNA chips, RT-PCR was performed in two independent experiments before therapy (lane 1, 3, 5, 7 and 9) and after therapy (lane 2, 4, 6, 8 and 10). Patient 1 (lanes 1 and 2), patient 2 (lanes 3 and 4), patient 3 (lanes 5 and 6), patient 4 (lane7 and 8) patient 5 (lanes 9 and 10). Patients 1, 2 and 4 were initially sensitive to chemotherapy (secondary resistant tumors). Human placenta was used as a positive control (lane 11) and mix without cDNA as negative control (lane 12). BMP7 was expressed in the 3 patients at relapse, but was not expressed in the 2 patients with refractory disease at diagnosis and after having failed on therapy. ß2microglobuline probe was used as internal reference. Figure 2B: Immunohistochemical analysis of one of the 2 patient’s tumor at diagnosis and at relapse, that expressed BMP7 at relapse (secondary resistant lymphoma).
    Human Bmp7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Olympus bmp 7
    Perioperative X-rays of a femoral non-union. Notes: Before non-union treatment according to the diamond concept ( A ) and 6 months postoperatively when consolidation was achieved ( B ). The intramedullary nail was removed, the non-union resected and the gap was filled with ABG (autologous bone graft), 3.3 mg <t>BMP-7</t> and a tricalcium phosphate bone graft (Vitoss; Stryker, Duisburg, Germany). Re-osteosynthesis was performed with an angled blade plate.
    Bmp 7, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Prospec bmp 7
    Immunofluorescence and functional in vitro analysis of <t>BMP-7–treated</t> hNEPT. A , top row: BMP-7–induced cell aggregates coexpress insulin (green) and C-PEP (C-peptide, red). Channel merge and DAPI nuclear staining (blue) are shown at the right. Bottom row: Nuclear PDX-1 (red)/cytoplasmic insulin (green). Channel merge and nuclear staining (blue) are shown at the right. Scale bar, 50 μm. B , top row, from left to right: INS (insulin, green)/GLU (glucagon, red); INS (insulin, green)/PPY (red); INS (insulin, green)/SST (red). Bottom row, from left to right: INS (insulin, green)/NKX6.1 (red); INS (green)/MAFA (red); and INS (green)/PDX-1 (red)/DAPI (blue) in a representative insulin − /PDX-1 + cluster. Scale bars, 50 μm for top row and pictures 1 and 2 on the bottom row. Picture 3, bottom row: 100 μm. C : GSIS of control and treated (BMP-7) hNEPT ( n = 5 preparations). x -axis: L1, low glucose 1 (2.5 mmol/L); H, high glucose (20 mmol/L); L2, low glucose 2 (2.5 mmol/L). y -axis: human C-peptide (ng/μg of DNA). A second exposure to low glucose was performed to rule out false-positive results caused by “dumping” (the nonphysiological release of insulin). Data are presented as the mean ± SD ( n = 5). n.s., no significance ( P > 0.05, two-tailed paired t test). *Statistical significance ( P
    Bmp 7, supplied by Prospec, used in various techniques. Bioz Stars score: 93/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology bmp 7
    Renal immunostaining for TGF- β 1 (a)–(c), fibronectin (d)–(f), collagen IV (g)–(i), and <t>BMP-7</t> (j)–(l) expression in normal or STZ-diabetic mice receiving 4 weeks of XCHT treatment ( n = 6 per group). Original magnification, 200x. Representative micrographs are shown.
    Bmp 7, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biotrend Chemicals bmp 7
    Immunohistochemical staining of differentially expressed gene products. Normal ovaries ( A , D , G , J , M ), serous papillary ovarian carcinoma ( B , E , H , K , N ), and serous papillary ovarian carcinoma metastatic to the omentum ( C , F , I , L , O ) tissues were stained with a mAb against the β1 integrin subunit ( A–C ), normal mouse IgG ( D–F ), and antibodies against: the β8 integrin subunit ( G–I ), <t>BMP-7</t> ( J–L ), and claudin-4 ( M–O ). Original magnifications, ×60.
    Bmp 7, supplied by Biotrend Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Aviva Systems bmp 7
    Immunohistochemistry for bone morphogenic protein (BMP; [a–c and g–l] 100× magnification; [d and f] 400× magnification). (a, d, g, and j) BMP-2, (b, e, h, and k) BMP-4, and (c, f, i, and l) <t>BMP-7.</t> IPB cells were strongly positively stained for BMP-4 and produced a negative result after staining for BMP-2 and BMP-7. IPB, inverted papilloma with new bone formation; IPC, inverted papilloma without bone formation (common type); NP, nasal polyp. *Bone tissue.
    Bmp 7, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ELISA analysis of serum BMP-7 levels in healthy subjects and patients with HCC. Healthy subjects (n=7); HCC stage I (n=3), II (n=12), IIIA (n=9) and IIIB (n=3). Tissues from healthy subjects were used as controls. **P

    Journal: Oncology Letters

    Article Title: Decreased BMP-7 and p-Smad1/5/8 expression, and increased levels of gremlin in hepatocellular carcinoma

    doi: 10.3892/ol.2018.8918

    Figure Lengend Snippet: ELISA analysis of serum BMP-7 levels in healthy subjects and patients with HCC. Healthy subjects (n=7); HCC stage I (n=3), II (n=12), IIIA (n=9) and IIIB (n=3). Tissues from healthy subjects were used as controls. **P

    Article Snippet: ELISA for cytokine analysis The serum BMP-7 level was examined by ELISA using a human BMP-7 ELISA kit according to manufacturer's protocol (RayBiotech Inc., GA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    The prodomain of BMP7 is not sufficient for maximal heterodimer activity. ( A ) Schematic illustration of ligands generated from native and chimeric BMP precursor proteins. ( B ) BMP RNAs were injected individually (200 pg) or together (100 pg of each RNA)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: The prodomain of BMP4 is necessary and sufficient to generate stable BMP4/7 heterodimers with enhanced bioactivity in vivo

    doi: 10.1073/pnas.1501449112

    Figure Lengend Snippet: The prodomain of BMP7 is not sufficient for maximal heterodimer activity. ( A ) Schematic illustration of ligands generated from native and chimeric BMP precursor proteins. ( B ) BMP RNAs were injected individually (200 pg) or together (100 pg of each RNA)

    Article Snippet: Membranes were probed with antibodies specific for HA (3F10, Roche) or the BMP7 prodomain (mAb2) ( ) and immunoreactive proteins were detected using Enhanced Chemiluminescence reagent (Pierce).

    Techniques: Activity Assay, Generated, Injection

    Effect of LIPUS stimulation on BMPs hASCs were treated by LIPUS at 20 and 30 mW cm −2 for 30 min daily, and the protein levels of BMP-2, BMP-6, BMP-7, and BMP-9 were detected using Western blot analysis. ( A ) Representative Western blot bands that displayed the protein expression of BMP-2, BMP-6, BMP-7, and BMP-9. ( B ) The protein levels of BMP-2, BMP-6, BMP-7, and BMP-9 relative to β-actin used as a loading control. Data are presented as mean ± SD ( n =3); * P

    Journal: Bioscience Reports

    Article Title: Low-intensity pulsed ultrasound stimulation facilitates in vitro osteogenic differentiation of human adipose-derived stem cells via up-regulation of heat shock protein (HSP)70, HSP90, and bone morphogenetic protein (BMP) signaling pathway

    doi: 10.1042/BSR20180087

    Figure Lengend Snippet: Effect of LIPUS stimulation on BMPs hASCs were treated by LIPUS at 20 and 30 mW cm −2 for 30 min daily, and the protein levels of BMP-2, BMP-6, BMP-7, and BMP-9 were detected using Western blot analysis. ( A ) Representative Western blot bands that displayed the protein expression of BMP-2, BMP-6, BMP-7, and BMP-9. ( B ) The protein levels of BMP-2, BMP-6, BMP-7, and BMP-9 relative to β-actin used as a loading control. Data are presented as mean ± SD ( n =3); * P

    Article Snippet: After determination of protein concentrations with a Bradford assay (Bio-Rad, Hercules, CA), equal amounts of total protein (10 μg each sample) were loaded onto 10% Tris-glycine gels, and electrophoresis was performed for 40 min. Then, the separated proteins were transferred to polyvinylidene difluoride membranes, followed by block with TBST (10 mmol l−1 Tris-HCl pH 7.5, 150 mmol ml−1 NaCl, and 0.1% Tween-20) buffer containing 5% nonfat milk for 1 h before incubation with primary antibodies against bone morphogenetic protein (BMP)-2, BMP-6, BMP-7, BMP-9, heat shock protein (HSP)27, HSP40, HSP70, HSP90, Runx2, OPN, OCN, p-Smad 1/5, Smad 5, Noggin, and β-actin (Cell Signal Technology, Beverly, MA, USA) for overnight at 4°C, followed by the addition of biotin-conjugated secondary antibodies.

    Techniques: Western Blot, Expressing

    Ratio of α-SMA, TGF-β1, BMP-7 and E-cadherin to GAPDH in the alendronate sodium control and liver fibrosis model groups. (−) Primary mouse hepatocytes (1×10 6 /dish) were cultured for 48 h in F12 medium containing 10% fetal bovine serum and 5 μ g/ml insulin until they had adhered. (+) Primary mouse hepatocytes (1×10 6 /dish) were cultured for 48 h in F12 medium containing 10% fetal bovine serum, 5 μ g/ml insulin and 50 μ g/mL alendronate sodium. TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; BMP-7, bone morphogenetic protein-7.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of alendronate sodium on the expression of mesenchymal-epithelial transition markers in mice with liver fibrosis

    doi: 10.3892/etm.2012.759

    Figure Lengend Snippet: Ratio of α-SMA, TGF-β1, BMP-7 and E-cadherin to GAPDH in the alendronate sodium control and liver fibrosis model groups. (−) Primary mouse hepatocytes (1×10 6 /dish) were cultured for 48 h in F12 medium containing 10% fetal bovine serum and 5 μ g/ml insulin until they had adhered. (+) Primary mouse hepatocytes (1×10 6 /dish) were cultured for 48 h in F12 medium containing 10% fetal bovine serum, 5 μ g/ml insulin and 50 μ g/mL alendronate sodium. TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; BMP-7, bone morphogenetic protein-7.

    Article Snippet: The primary antibodies were as follows: anti-α-SMA (1:2,000 dilution; Dako, Carpinteria, CA, USA), anti-TGF-β1 (1:2,000 dilution; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-E-cadherin (1:2,000 dilution; USA), anti-BMP-7 (1:1,500 dilution; Cell Signaling Technology, Danvers, MA, USA), anti-FSP1 (1:2,000 dilution; Santa Cruz Biotechnology) and anti-GAPDH (1:2,000 dilution; Sigma-Aldrich).

    Techniques: Cell Culture

    RT-PCR results demonstrating the effect of CCl 4 -induced mouse liver fibrosis on the expression of TGF-β1, α-SMA, N-cadherin, FSP1, BMP-7 and E-cadherin mRNA during EMT in (A) the control group, (B) the liver fibrosis model group and (C) the alendronate sodium-treated group during chronic exposure to TGF-β1 (5 ng/ml) from 24 to 96 h. TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; FSP1, fibroblast-specific protein 1; BMP-7, bone morphogenetic protein-7; EMT, epithelial-mesenchymal transition.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of alendronate sodium on the expression of mesenchymal-epithelial transition markers in mice with liver fibrosis

    doi: 10.3892/etm.2012.759

    Figure Lengend Snippet: RT-PCR results demonstrating the effect of CCl 4 -induced mouse liver fibrosis on the expression of TGF-β1, α-SMA, N-cadherin, FSP1, BMP-7 and E-cadherin mRNA during EMT in (A) the control group, (B) the liver fibrosis model group and (C) the alendronate sodium-treated group during chronic exposure to TGF-β1 (5 ng/ml) from 24 to 96 h. TGF-β1, transforming growth factor-β1; α-SMA, α-smooth muscle actin; FSP1, fibroblast-specific protein 1; BMP-7, bone morphogenetic protein-7; EMT, epithelial-mesenchymal transition.

    Article Snippet: The primary antibodies were as follows: anti-α-SMA (1:2,000 dilution; Dako, Carpinteria, CA, USA), anti-TGF-β1 (1:2,000 dilution; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-E-cadherin (1:2,000 dilution; USA), anti-BMP-7 (1:1,500 dilution; Cell Signaling Technology, Danvers, MA, USA), anti-FSP1 (1:2,000 dilution; Santa Cruz Biotechnology) and anti-GAPDH (1:2,000 dilution; Sigma-Aldrich).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    BMP7 mediates KDM5C-induced migration and invasion in HCC cells. a Silenced BMP7 protein expression in MHCC97L-pSuper-shKDM5C cells was measured by Western blotting. b , Silenced BMP7 mRNA expression in MHCC97L-pSuper-shKDM5C cells was measured by qRT-PCR. c MHCC97L-pSuper-shKDM5C cells treated with siBMP7 were subjected to Transwell migration (top), and Matrigel invasion assays (bottom), quantification of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown as proportions of their vector controls. **, P

    Journal: BMC Cancer

    Article Title: Lysine-specific demethylase 5C promotes hepatocellular carcinoma cell invasion through inhibition BMP7 expression

    doi: 10.1186/s12885-015-1798-4

    Figure Lengend Snippet: BMP7 mediates KDM5C-induced migration and invasion in HCC cells. a Silenced BMP7 protein expression in MHCC97L-pSuper-shKDM5C cells was measured by Western blotting. b , Silenced BMP7 mRNA expression in MHCC97L-pSuper-shKDM5C cells was measured by qRT-PCR. c MHCC97L-pSuper-shKDM5C cells treated with siBMP7 were subjected to Transwell migration (top), and Matrigel invasion assays (bottom), quantification of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown as proportions of their vector controls. **, P

    Article Snippet: E-cadherin, N-cadherin, vimentin, BMP7, and β-actin antibodies were from Cell Signaling technology (Danvers, MA, USA).

    Techniques: Migration, Expressing, Western Blot, Quantitative RT-PCR, Plasmid Preparation

    KDM5C downregulates BMP7 expression in HCC cells. a Supervised hierarchical clustering of the genes differentially expressed after KDM5C overexpression in L02 cells. b Gene set enrichment analysis was carried out using ConceptGen. c Protein level of BMP7 was measured by Western blotting assay in L02-pBabe-KDM5C and its control cells. d , mRNA level of BMP7 was measured by qRT-PCR assay in L02-pBabe-KDM5C and its control cells. e Protein level of BMP7 was measured by Western blotting assay in MHCC97L-pSuper-shKDM5C and its control cells. f mRNA level of BMP7 was measured by qRT-PCR assay in MHCC97L-pSuper-shKDM5C and its control cells. **, P

    Journal: BMC Cancer

    Article Title: Lysine-specific demethylase 5C promotes hepatocellular carcinoma cell invasion through inhibition BMP7 expression

    doi: 10.1186/s12885-015-1798-4

    Figure Lengend Snippet: KDM5C downregulates BMP7 expression in HCC cells. a Supervised hierarchical clustering of the genes differentially expressed after KDM5C overexpression in L02 cells. b Gene set enrichment analysis was carried out using ConceptGen. c Protein level of BMP7 was measured by Western blotting assay in L02-pBabe-KDM5C and its control cells. d , mRNA level of BMP7 was measured by qRT-PCR assay in L02-pBabe-KDM5C and its control cells. e Protein level of BMP7 was measured by Western blotting assay in MHCC97L-pSuper-shKDM5C and its control cells. f mRNA level of BMP7 was measured by qRT-PCR assay in MHCC97L-pSuper-shKDM5C and its control cells. **, P

    Article Snippet: E-cadherin, N-cadherin, vimentin, BMP7, and β-actin antibodies were from Cell Signaling technology (Danvers, MA, USA).

    Techniques: Expressing, Over Expression, Western Blot, Quantitative RT-PCR

    KDM5C regulates BMP7 expression through H3K4 trimethylation. a The abundance of H3 lysine methylation was assessed in HCC cells with KDM5C overexpression or shRNA by Western blotting using whole-cell lysate; total H3 and β-actin were used as a loading control. b Schematic presentation of three regions relative to the BMP7 transcriptional start site used as primers to test histone occupied abundance. c and d qChIP was performed to assess H3K4me3 occupancy in L02-pBabe-KDM5C and its control cells. IgG was used as negative control. e and f , qChIP was performed to assess H3K4me3 occupancy in MHCC97L-pSuper-shKDM5C and its control cells. IgG was used as negative control. “Percentage of input” indicates the ratio of DNA fragment of each promoter region bound by H3K4me3 to the total amount of input DNA fragment without H3K4me3 antibody pull-down. **, P

    Journal: BMC Cancer

    Article Title: Lysine-specific demethylase 5C promotes hepatocellular carcinoma cell invasion through inhibition BMP7 expression

    doi: 10.1186/s12885-015-1798-4

    Figure Lengend Snippet: KDM5C regulates BMP7 expression through H3K4 trimethylation. a The abundance of H3 lysine methylation was assessed in HCC cells with KDM5C overexpression or shRNA by Western blotting using whole-cell lysate; total H3 and β-actin were used as a loading control. b Schematic presentation of three regions relative to the BMP7 transcriptional start site used as primers to test histone occupied abundance. c and d qChIP was performed to assess H3K4me3 occupancy in L02-pBabe-KDM5C and its control cells. IgG was used as negative control. e and f , qChIP was performed to assess H3K4me3 occupancy in MHCC97L-pSuper-shKDM5C and its control cells. IgG was used as negative control. “Percentage of input” indicates the ratio of DNA fragment of each promoter region bound by H3K4me3 to the total amount of input DNA fragment without H3K4me3 antibody pull-down. **, P

    Article Snippet: E-cadherin, N-cadherin, vimentin, BMP7, and β-actin antibodies were from Cell Signaling technology (Danvers, MA, USA).

    Techniques: Expressing, Methylation, Over Expression, shRNA, Western Blot, Negative Control

    Time course of Smad 1/5/8 phosphorylation following addition of BMP7 ± BDNF

    Journal:

    Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

    doi: 10.1111/j.1471-4159.2009.05990.x

    Figure Lengend Snippet: Time course of Smad 1/5/8 phosphorylation following addition of BMP7 ± BDNF

    Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

    Techniques:

    Hypoglycemia increases the enhancement of nuclear and cytoplasmic P-Smad levels produced by both BMP7 alone and a BMP7 + BDNF combination

    Journal:

    Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

    doi: 10.1111/j.1471-4159.2009.05990.x

    Figure Lengend Snippet: Hypoglycemia increases the enhancement of nuclear and cytoplasmic P-Smad levels produced by both BMP7 alone and a BMP7 + BDNF combination

    Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

    Techniques: Produced

    BDNF-induced increases in phosphorylated Akt and Erk are not modified by BMP7 or a CK2 inhibitor, TBB

    Journal:

    Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

    doi: 10.1111/j.1471-4159.2009.05990.x

    Figure Lengend Snippet: BDNF-induced increases in phosphorylated Akt and Erk are not modified by BMP7 or a CK2 inhibitor, TBB

    Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

    Techniques: Modification

    Localization of catalytic subunits of CK2 in septal neurons and activation of CK2 by BDNF and BMP7

    Journal:

    Article Title: Casein kinase II contributes to the synergistic effects of BMP7 and BDNF on Smad 1/5/8 phosphorylation in septal neurons under hypoglycemic stress

    doi: 10.1111/j.1471-4159.2009.05990.x

    Figure Lengend Snippet: Localization of catalytic subunits of CK2 in septal neurons and activation of CK2 by BDNF and BMP7

    Article Snippet: Trophic factors: recombinant human BMP6 and BMP7 (Calbiochem, LaJolla, CA, USA); recombinant human NGF and BDNF (Alomone Labs, Jerusalem, Israel).

    Techniques: Activation Assay

    Western blot analyses depicting S100A4, TGF- β , BMP-7, Smad2/3, Smad1/5/8, and p16 INK4a expression in vivo after rhEPO treatment in UUO model. S100A4, TGF- β , Smad2/3, and p16 INK4a expression were significantly higher in UUO mice on days 3, 7, and 14 ( P

    Journal: BioMed Research International

    Article Title: Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction

    doi: 10.1155/2013/308130

    Figure Lengend Snippet: Western blot analyses depicting S100A4, TGF- β , BMP-7, Smad2/3, Smad1/5/8, and p16 INK4a expression in vivo after rhEPO treatment in UUO model. S100A4, TGF- β , Smad2/3, and p16 INK4a expression were significantly higher in UUO mice on days 3, 7, and 14 ( P

    Article Snippet: Positive Correlation between TGF-β , Smad2/3, and p16INK4a but Negative Correlation between BMP-7, Smad1/5/8, and p16INK4a in UUO Kidneys Obstructed kidneys had significantly increased TGF-β , Smad2/3, and p16INK4a but decreased BMP-7, Smad1/5/8 protein, and mRNA.

    Techniques: Western Blot, Expressing, In Vivo, Mouse Assay

    Real-time RT-PCR for TGF- β , BMP-7, Smad3, Smad8, and p16 INK4a mRNA expression in vivo after rhEPO treatment in UUO model. TGF- β , Smad3, and p16 INK4a mRNA expression show markedly progressive upregulation in UUO mice on days 3, 7, and 14 ( P

    Journal: BioMed Research International

    Article Title: Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction

    doi: 10.1155/2013/308130

    Figure Lengend Snippet: Real-time RT-PCR for TGF- β , BMP-7, Smad3, Smad8, and p16 INK4a mRNA expression in vivo after rhEPO treatment in UUO model. TGF- β , Smad3, and p16 INK4a mRNA expression show markedly progressive upregulation in UUO mice on days 3, 7, and 14 ( P

    Article Snippet: Positive Correlation between TGF-β , Smad2/3, and p16INK4a but Negative Correlation between BMP-7, Smad1/5/8, and p16INK4a in UUO Kidneys Obstructed kidneys had significantly increased TGF-β , Smad2/3, and p16INK4a but decreased BMP-7, Smad1/5/8 protein, and mRNA.

    Techniques: Quantitative RT-PCR, Expressing, In Vivo, Mouse Assay

    Representative photographs of kidney sections stained with TGF- β , BMP-7, Smad2/3, and p16 INK4a in UUO model. In sham kidneys, no or little TGF- β labelling was seen. Advanced increased TGF- β labelling was seen in the interstitium area in the obstructed kidneys compared with the sham at days 3, 7, and 14. In contrast, decrease of TGF- β staining was observed in UUO mice with rhEPO treatment. In contrast, BMP-7 was demonstrated in the cytoplasm of TEC in sham kidneys, whereas the labeling of BMP-7 was decreased in cytoplasm of TEC particularly in dilated and atrophic tubules of the placebo treated UUO kidneys since day 3 after UUO and progressive loss until day 14. rhEPO treatment in mice with UUO demonstrated the significantly preserved cytoplasm staining intensity of BMP-7 in the obstructed kidneys. Moreover, no Smad2/3 and p16 INK4a staining was seen in TEC in sham kidneys. Smad2/3 and p16 INK4a are detected at the nucleus of TEC with weak cytoplasm staining particularly in dilated and atrophic tubules of the UUO kidneys since days 3, 7, and 14. In contrast, rhEPO treatment in mice with UUO demonstrated the significantly attenuated nucleus and cytoplasm staining intensity of Smad2/3 and p16 INK4a in the obstructed kidneys. Original magnifications ×400.

    Journal: BioMed Research International

    Article Title: Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction

    doi: 10.1155/2013/308130

    Figure Lengend Snippet: Representative photographs of kidney sections stained with TGF- β , BMP-7, Smad2/3, and p16 INK4a in UUO model. In sham kidneys, no or little TGF- β labelling was seen. Advanced increased TGF- β labelling was seen in the interstitium area in the obstructed kidneys compared with the sham at days 3, 7, and 14. In contrast, decrease of TGF- β staining was observed in UUO mice with rhEPO treatment. In contrast, BMP-7 was demonstrated in the cytoplasm of TEC in sham kidneys, whereas the labeling of BMP-7 was decreased in cytoplasm of TEC particularly in dilated and atrophic tubules of the placebo treated UUO kidneys since day 3 after UUO and progressive loss until day 14. rhEPO treatment in mice with UUO demonstrated the significantly preserved cytoplasm staining intensity of BMP-7 in the obstructed kidneys. Moreover, no Smad2/3 and p16 INK4a staining was seen in TEC in sham kidneys. Smad2/3 and p16 INK4a are detected at the nucleus of TEC with weak cytoplasm staining particularly in dilated and atrophic tubules of the UUO kidneys since days 3, 7, and 14. In contrast, rhEPO treatment in mice with UUO demonstrated the significantly attenuated nucleus and cytoplasm staining intensity of Smad2/3 and p16 INK4a in the obstructed kidneys. Original magnifications ×400.

    Article Snippet: Positive Correlation between TGF-β , Smad2/3, and p16INK4a but Negative Correlation between BMP-7, Smad1/5/8, and p16INK4a in UUO Kidneys Obstructed kidneys had significantly increased TGF-β , Smad2/3, and p16INK4a but decreased BMP-7, Smad1/5/8 protein, and mRNA.

    Techniques: Staining, Mouse Assay, Labeling

    Localization of BMP-2, BMP-4, and BMP-7 antigens within defect sites with immobilized anti-BMP-2 mAb following in vivo implantation and retrieval after 8 weeks. (a) Representative CLSM images of titanium, alginate, and ACS groups with immobilized anti-BMP-2 mAb harvested from calvarial defects after 8 weeks. The immunofluorescence results revealed the capacity of the murine mAb to attract and hold BMP-2, -4, and -7 ligands. Scaffolds immobilized with nonspecific isotype mAb failed to show any positive staining. (b) Quantitative analysis of red fluorescence intensity of the images shown in (a). N = 4 for each group. * P

    Journal: BioMed Research International

    Article Title: Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering

    doi: 10.1155/2014/940860

    Figure Lengend Snippet: Localization of BMP-2, BMP-4, and BMP-7 antigens within defect sites with immobilized anti-BMP-2 mAb following in vivo implantation and retrieval after 8 weeks. (a) Representative CLSM images of titanium, alginate, and ACS groups with immobilized anti-BMP-2 mAb harvested from calvarial defects after 8 weeks. The immunofluorescence results revealed the capacity of the murine mAb to attract and hold BMP-2, -4, and -7 ligands. Scaffolds immobilized with nonspecific isotype mAb failed to show any positive staining. (b) Quantitative analysis of red fluorescence intensity of the images shown in (a). N = 4 for each group. * P

    Article Snippet: For immunofluorescence staining, deparaffinized samples were treated with 3% H2 O2 , followed by a blocking buffer (1% BSA and 0.25% Triton X-100 in PBS), stained with rabbit polyclonal anti-BMP-2, BMP-4, and BMP-7 antibodies (Abcam, Cambridge, MA) at 4°C overnight, and detected using Alexa Fluor-conjugated secondary antibody (1 : 200 dilution; Invitrogen) using CLSM (Fluoview FV10i, Olympus Corp., Tokyo, Japan).

    Techniques: In Vivo, Confocal Laser Scanning Microscopy, Immunofluorescence, Staining, Fluorescence

    Immunohistochemical analysis of bone morphogenetic protein-7 expression in rats of each group (× 400). A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats

    doi: 10.3748/wjg.v20.i40.14875

    Figure Lengend Snippet: Immunohistochemical analysis of bone morphogenetic protein-7 expression in rats of each group (× 400). A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated

    Article Snippet: Hyaluronic acid (HA), hydroxyproline (Hyp), and TGF-β1 detection kits (products of Nanjing Jiancheng Co. Ltd., Batch No. 20110228, 20110215, and 20110218, respectively); a reverse-transcription kit (product of Canada Fermentas MBI, Batch No. 00087036); an electrochemiluminescence kit (product of America Millipore Company, Batch No. 1219101); a whole protein extraction kit (product of Nanjing Keygen Biotech Co., Ltd., Batch No. KGP250); a BCA protein quantification kit (product of America Thermo Scientific Company, Batch No. 23227); and Gremlin and BMP-7 primary antibodies (product of England Abcam Company, Batch No. ad90670 and ab56023) were utilized in the study.

    Techniques: Immunohistochemistry, Expressing

    Comparison of mRNA and protein expression of BMP-7 between the groups

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats

    doi: 10.3748/wjg.v20.i40.14875

    Figure Lengend Snippet: Comparison of mRNA and protein expression of BMP-7 between the groups

    Article Snippet: Hyaluronic acid (HA), hydroxyproline (Hyp), and TGF-β1 detection kits (products of Nanjing Jiancheng Co. Ltd., Batch No. 20110228, 20110215, and 20110218, respectively); a reverse-transcription kit (product of Canada Fermentas MBI, Batch No. 00087036); an electrochemiluminescence kit (product of America Millipore Company, Batch No. 1219101); a whole protein extraction kit (product of Nanjing Keygen Biotech Co., Ltd., Batch No. KGP250); a BCA protein quantification kit (product of America Thermo Scientific Company, Batch No. 23227); and Gremlin and BMP-7 primary antibodies (product of England Abcam Company, Batch No. ad90670 and ab56023) were utilized in the study.

    Techniques: Expressing

    Western blot analysis of bone morphogenetic protein-7 expression in rats of each group. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group. BMP-7: Bone morphogenetic

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats

    doi: 10.3748/wjg.v20.i40.14875

    Figure Lengend Snippet: Western blot analysis of bone morphogenetic protein-7 expression in rats of each group. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group. BMP-7: Bone morphogenetic

    Article Snippet: Hyaluronic acid (HA), hydroxyproline (Hyp), and TGF-β1 detection kits (products of Nanjing Jiancheng Co. Ltd., Batch No. 20110228, 20110215, and 20110218, respectively); a reverse-transcription kit (product of Canada Fermentas MBI, Batch No. 00087036); an electrochemiluminescence kit (product of America Millipore Company, Batch No. 1219101); a whole protein extraction kit (product of Nanjing Keygen Biotech Co., Ltd., Batch No. KGP250); a BCA protein quantification kit (product of America Thermo Scientific Company, Batch No. 23227); and Gremlin and BMP-7 primary antibodies (product of England Abcam Company, Batch No. ad90670 and ab56023) were utilized in the study.

    Techniques: Western Blot, Expressing

    Agmatine treatment modulated the BMP- 2/4/7 expressions after SCI. Quantification of BMP- 2/4/7 protein expressions was determined by western blot analysis. The bar graph represents densitometry measurement of BMP- 2/4/7 protein expressions. (A) BMP- 2 protein expression was significantly higher in the Agm treated group ( n  = 5) compared with the EC group ( n  = 5) at 1 and 7 DPI. (B) BMP- 7 protein expression was higher in the Agm treated group ( n  = 5) compared with the EC group ( n  = 5) at 1, 7, 14, and 35 DPI and the increase was significant at 7    14 DPI. (C) BMP- 4 expression was significantly lower in the Agm treated group ( n  = 5) compared with the EC group ( n  = 5) at 7, 14    35 DPI following SCI. †,  p

    Journal: PLoS ONE

    Article Title: The Multifaceted Effects of Agmatine on Functional Recovery after Spinal Cord Injury through Modulations of BMP-2/4/7 Expressions in Neurons and Glial Cells

    doi: 10.1371/journal.pone.0053911

    Figure Lengend Snippet: Agmatine treatment modulated the BMP- 2/4/7 expressions after SCI. Quantification of BMP- 2/4/7 protein expressions was determined by western blot analysis. The bar graph represents densitometry measurement of BMP- 2/4/7 protein expressions. (A) BMP- 2 protein expression was significantly higher in the Agm treated group ( n  = 5) compared with the EC group ( n  = 5) at 1 and 7 DPI. (B) BMP- 7 protein expression was higher in the Agm treated group ( n  = 5) compared with the EC group ( n  = 5) at 1, 7, 14, and 35 DPI and the increase was significant at 7 14 DPI. (C) BMP- 4 expression was significantly lower in the Agm treated group ( n  = 5) compared with the EC group ( n  = 5) at 7, 14 35 DPI following SCI. †, p

    Article Snippet: The following primary antibodies were used overnight at 4°C: anti-mouse GFAP (1∶1000, monoclonal antibody; Thermo), anti-rabbit S100β (1∶1000, monoclonal antibody; Abcam), anti-mouse BMP-2 (1∶1000, monoclonal antibody; Abcam), anti-mouse BMP-4 (1∶1000, polyclonal antibody; Santa-cruz), anti-rabbit BMP-7 (1∶1000, monoclonal antibody; Abcam), anti-mouse MAP-2 (1∶1000, monoclonal antibody; Sigma), and anti-goat Olig-2 (1∶1000, polyclonal antibody; Santa-cruz).

    Techniques: Western Blot, Expressing

    CAST analysis showed that agmatine treatment modulated BMP- 2/7 expressions in neurons oligodendrocytes and BMP- 4 expressions in astrocytes oligodendrocytes following SCI. (A) Stereological counting of BMP- 2 + /MAP-2 + and (B) BMP- 2 + /Olig-2 + cells in the injured spinal cord (Th 8–Th 10 segments). The number of BMP- 2 + /MAP-2 + and BMP- 2 + /Olig-2 + cells were significantly increased in the Agm treated group ( n = 5) compared with the EC group ( n = 5) at 7 35 DPI and 7 14 DPI (C) BMP- 7 + /MAP-2 + and (D) BMP- 7 + /Olig-2 + cells in the Th 8–Th 10 segments of the injured spinal cord. The number of BMP-7 + /MAP-2 + and BMP-7 + /Olig-2 + cells were significantly increased in the Agm treated group ( n = 5) compared with the EC group ( n = 5) at 7 14 days and 7 days respectively following SCI. (E) BMP- 4 + /GFAP + and (F) BMP- 4 + /Olig-2 + cells in Th 8–Th 10 segments of the injured spinal cord. The number of BMP- 4 + /GFAP + and BMP- 4 + /Olig-2 + cells were decreased at all the time periods in the Agm treated group ( n = 5) compared with the EC group ( n = 5) and the decrease was significant at 7 14 days and 14 35 days respectively following SCI. †, p

    Journal: PLoS ONE

    Article Title: The Multifaceted Effects of Agmatine on Functional Recovery after Spinal Cord Injury through Modulations of BMP-2/4/7 Expressions in Neurons and Glial Cells

    doi: 10.1371/journal.pone.0053911

    Figure Lengend Snippet: CAST analysis showed that agmatine treatment modulated BMP- 2/7 expressions in neurons oligodendrocytes and BMP- 4 expressions in astrocytes oligodendrocytes following SCI. (A) Stereological counting of BMP- 2 + /MAP-2 + and (B) BMP- 2 + /Olig-2 + cells in the injured spinal cord (Th 8–Th 10 segments). The number of BMP- 2 + /MAP-2 + and BMP- 2 + /Olig-2 + cells were significantly increased in the Agm treated group ( n = 5) compared with the EC group ( n = 5) at 7 35 DPI and 7 14 DPI (C) BMP- 7 + /MAP-2 + and (D) BMP- 7 + /Olig-2 + cells in the Th 8–Th 10 segments of the injured spinal cord. The number of BMP-7 + /MAP-2 + and BMP-7 + /Olig-2 + cells were significantly increased in the Agm treated group ( n = 5) compared with the EC group ( n = 5) at 7 14 days and 7 days respectively following SCI. (E) BMP- 4 + /GFAP + and (F) BMP- 4 + /Olig-2 + cells in Th 8–Th 10 segments of the injured spinal cord. The number of BMP- 4 + /GFAP + and BMP- 4 + /Olig-2 + cells were decreased at all the time periods in the Agm treated group ( n = 5) compared with the EC group ( n = 5) and the decrease was significant at 7 14 days and 14 35 days respectively following SCI. †, p

    Article Snippet: The following primary antibodies were used overnight at 4°C: anti-mouse GFAP (1∶1000, monoclonal antibody; Thermo), anti-rabbit S100β (1∶1000, monoclonal antibody; Abcam), anti-mouse BMP-2 (1∶1000, monoclonal antibody; Abcam), anti-mouse BMP-4 (1∶1000, polyclonal antibody; Santa-cruz), anti-rabbit BMP-7 (1∶1000, monoclonal antibody; Abcam), anti-mouse MAP-2 (1∶1000, monoclonal antibody; Sigma), and anti-goat Olig-2 (1∶1000, polyclonal antibody; Santa-cruz).

    Techniques:

    Confocal microscopic images of BMP- 2/4/7 expressions in neurons, oligodendrocytes and astrocytes after agmatine treatment following SCI. (A) Dual immunofluorescence was done to localize the BMP- 2 expression in neurons (MAP-2) and oligodendrocytes (Olig-2) after SCI. The BMP- 2 + /MAP- 2 +   BMP- 2 + /Olig-2 +  cells around the lesion site were higher in the Agm treated group ( n  = 4) compared with the EC group ( n  = 4) at7 DPI. Scale bars: 50 µm. (B) The immunostaining of BMP- 7 (red) with MAP-2 (green) showed increased number of BMP- 7 + /MAP-2 +  cells in the Agm treated group ( n  = 5) when compared with the EC group ( n  = 5) at 7 days after SCI. The immunostaining of BMP- 7 with Olig-2 showed an increased number of BMP- 7 + /Olig-2 +  cells in the Agm treated group ( n  = 5) compared to EC group ( n  = 5) at 14 DPI. Scale bars: 10 µm. (C) The immuno co-localization of BMP- 4 +  cells in astrocytes (GFAP) showed reduced number of BMP- 4 + /GFAP +  cellsaround the lesion site in the Agm treated group ( n  = 4) compared with the EC group ( n  = 4) at7 DPI. Whereas, BMP- 4 + /Olig-2 +  cells were higher in the Agm treated group compared with EC at 35 DPI. The scale bars: 10 µm.

    Journal: PLoS ONE

    Article Title: The Multifaceted Effects of Agmatine on Functional Recovery after Spinal Cord Injury through Modulations of BMP-2/4/7 Expressions in Neurons and Glial Cells

    doi: 10.1371/journal.pone.0053911

    Figure Lengend Snippet: Confocal microscopic images of BMP- 2/4/7 expressions in neurons, oligodendrocytes and astrocytes after agmatine treatment following SCI. (A) Dual immunofluorescence was done to localize the BMP- 2 expression in neurons (MAP-2) and oligodendrocytes (Olig-2) after SCI. The BMP- 2 + /MAP- 2 + BMP- 2 + /Olig-2 + cells around the lesion site were higher in the Agm treated group ( n  = 4) compared with the EC group ( n  = 4) at7 DPI. Scale bars: 50 µm. (B) The immunostaining of BMP- 7 (red) with MAP-2 (green) showed increased number of BMP- 7 + /MAP-2 + cells in the Agm treated group ( n  = 5) when compared with the EC group ( n  = 5) at 7 days after SCI. The immunostaining of BMP- 7 with Olig-2 showed an increased number of BMP- 7 + /Olig-2 + cells in the Agm treated group ( n  = 5) compared to EC group ( n  = 5) at 14 DPI. Scale bars: 10 µm. (C) The immuno co-localization of BMP- 4 + cells in astrocytes (GFAP) showed reduced number of BMP- 4 + /GFAP + cellsaround the lesion site in the Agm treated group ( n  = 4) compared with the EC group ( n  = 4) at7 DPI. Whereas, BMP- 4 + /Olig-2 + cells were higher in the Agm treated group compared with EC at 35 DPI. The scale bars: 10 µm.

    Article Snippet: The following primary antibodies were used overnight at 4°C: anti-mouse GFAP (1∶1000, monoclonal antibody; Thermo), anti-rabbit S100β (1∶1000, monoclonal antibody; Abcam), anti-mouse BMP-2 (1∶1000, monoclonal antibody; Abcam), anti-mouse BMP-4 (1∶1000, polyclonal antibody; Santa-cruz), anti-rabbit BMP-7 (1∶1000, monoclonal antibody; Abcam), anti-mouse MAP-2 (1∶1000, monoclonal antibody; Sigma), and anti-goat Olig-2 (1∶1000, polyclonal antibody; Santa-cruz).

    Techniques: Immunofluorescence, Expressing, Immunostaining

    Dysregulated expression of molecules mediating Langerhans cell differentiation in the peri-implant mucosa.  (A)  Immunofluorescence cross sections of peri-implant and gingival tissues stained 4 weeks after implantation against TGF-β1 (red), bone morphogenetic protein 7 (BMP7) (green), and hoechst (blue). Negative control represents staining with the secondary antibody only. Representative immunofluorescence images of two independent experiments are shown. In each experiment, at least three mice were examined.  (B)  Bar graphs represent the mean field staining intensity of TGF-β1 and BMP7 in the epithelium and submucosa, respectively, shown as the mean ± SEM. The average pixel intensity was calculated for each slide using ImageJ software by countering the epithelium or the submucosa from nine different slides for each group ( n  = 3 mice per group). Representative images of two independent experiments are shown.  (C)  Four weeks after implant insertion, TGF-β1 and BMP7 mRNA expressions were quantified in the epithelium and submucosa, respectively, by RT- q PCR. Graph presents the fold change in gene expression normalized to non-implanted mice and presented as the mean ± SEM (at least 10 mice per group were analyzed). Data are representative of two pooled independent experiments.  (D)  Four weeks after teeth extraction, immunofluorescence cross sections of the alveolar process at extraction sites were stained as described in  (A) . Representative images of one out of two independent experiments are provided, three mice were analyzed in each experiment.  (E)  Graph presents the fold change in gene expression of activing-like kinase 3 (ALK3), ALK5, CCL20, CCL2, and GM-CSF in the epithelium, normalized to non-implanted mice 4 weeks after implantation, presented as the mean ± SEM ( n  = 6 mice per group). Data are representative of one out of two independent experiments. * p

    Journal: Frontiers in Immunology

    Article Title: Impaired Differentiation of Langerhans Cells in the Murine Oral Epithelium Adjacent to Titanium Dental Implants

    doi: 10.3389/fimmu.2018.01712

    Figure Lengend Snippet: Dysregulated expression of molecules mediating Langerhans cell differentiation in the peri-implant mucosa. (A) Immunofluorescence cross sections of peri-implant and gingival tissues stained 4 weeks after implantation against TGF-β1 (red), bone morphogenetic protein 7 (BMP7) (green), and hoechst (blue). Negative control represents staining with the secondary antibody only. Representative immunofluorescence images of two independent experiments are shown. In each experiment, at least three mice were examined. (B) Bar graphs represent the mean field staining intensity of TGF-β1 and BMP7 in the epithelium and submucosa, respectively, shown as the mean ± SEM. The average pixel intensity was calculated for each slide using ImageJ software by countering the epithelium or the submucosa from nine different slides for each group ( n  = 3 mice per group). Representative images of two independent experiments are shown. (C) Four weeks after implant insertion, TGF-β1 and BMP7 mRNA expressions were quantified in the epithelium and submucosa, respectively, by RT- q PCR. Graph presents the fold change in gene expression normalized to non-implanted mice and presented as the mean ± SEM (at least 10 mice per group were analyzed). Data are representative of two pooled independent experiments. (D) Four weeks after teeth extraction, immunofluorescence cross sections of the alveolar process at extraction sites were stained as described in (A) . Representative images of one out of two independent experiments are provided, three mice were analyzed in each experiment. (E) Graph presents the fold change in gene expression of activing-like kinase 3 (ALK3), ALK5, CCL20, CCL2, and GM-CSF in the epithelium, normalized to non-implanted mice 4 weeks after implantation, presented as the mean ± SEM ( n  = 6 mice per group). Data are representative of one out of two independent experiments. * p

    Article Snippet: The cross sections, as well as the separated epithelium, were washed three times in PBS, blocked in a blocking buffer (5% FCS, 0.1% Triton X-100 in PBS) for 1 h at room temperature, and incubated with primary antibodies: goat anti-langerin (E-17, Santa Cruz Biotechnology), rat anti-MHCII (M5/114.15.2, BioLegend), rabbit anti-TGF-β1 (ab92486 Abcam), and mouse anti-BMP-7 (ab54904 Abcam) overnight at 4°C.

    Techniques: Expressing, Cell Differentiation, Immunofluorescence, Staining, Negative Control, Mouse Assay, Software, Polymerase Chain Reaction

    miR-4739 over-expression results in pleural fibrosis in mice. (a) Sequences of has-miR-4739 and the putative target sequence in the mouse or rat BMP-7 mRNA (wild-type) or an engineered mutant of this sequence (mutant) for the luciferase activity assay. n = 3, *P

    Journal: EBioMedicine

    Article Title: miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7

    doi: 10.1016/j.ebiom.2019.02.057

    Figure Lengend Snippet: miR-4739 over-expression results in pleural fibrosis in mice. (a) Sequences of has-miR-4739 and the putative target sequence in the mouse or rat BMP-7 mRNA (wild-type) or an engineered mutant of this sequence (mutant) for the luciferase activity assay. n = 3, *P

    Article Snippet: Antibodies against collagen-I (Cat#14695-1-AP), BMP-7 (Cat#12221-1-AP), α-smooth muscle actin (α-SMA) (Cat#55135-1-AP), TGF-β1 (Cat#21898-1-AP) and GAPDH (Cat#60004–1-lg) were purchased from Proteintech (Rosemont, IL, USA).

    Techniques: Over Expression, Mouse Assay, Sequencing, Mutagenesis, Luciferase, Activity Assay

    BMP-7 reserved bleomycin induced pleural fibrosis in mice. (a-b) Rat primary PMCs were treated with or without bleomycin (0.2 μg/ml) in the presence or absence of BMP-7 (0.1 μg/ml) for 24 h. (a) Changes of protein levels of p-Smad2/3 and p-Smad1/5/9 were measured by Western blotting. n = 3, *P

    Journal: EBioMedicine

    Article Title: miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7

    doi: 10.1016/j.ebiom.2019.02.057

    Figure Lengend Snippet: BMP-7 reserved bleomycin induced pleural fibrosis in mice. (a-b) Rat primary PMCs were treated with or without bleomycin (0.2 μg/ml) in the presence or absence of BMP-7 (0.1 μg/ml) for 24 h. (a) Changes of protein levels of p-Smad2/3 and p-Smad1/5/9 were measured by Western blotting. n = 3, *P

    Article Snippet: Antibodies against collagen-I (Cat#14695-1-AP), BMP-7 (Cat#12221-1-AP), α-smooth muscle actin (α-SMA) (Cat#55135-1-AP), TGF-β1 (Cat#21898-1-AP) and GAPDH (Cat#60004–1-lg) were purchased from Proteintech (Rosemont, IL, USA).

    Techniques: Mouse Assay, Western Blot

    miR-4739 targets against BMP-7 and breaks the balance between Smad1/5/9 and Smad2/3 signaling. (a) Human PMCs were transducted with recombinant lentivirus vector containing miR-4739 or scrambled negative control (vector control) plasmids. After 96 h, changes of BMP-7 protein levels measured by Western blotting. n = 3, # P

    Journal: EBioMedicine

    Article Title: miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7

    doi: 10.1016/j.ebiom.2019.02.057

    Figure Lengend Snippet: miR-4739 targets against BMP-7 and breaks the balance between Smad1/5/9 and Smad2/3 signaling. (a) Human PMCs were transducted with recombinant lentivirus vector containing miR-4739 or scrambled negative control (vector control) plasmids. After 96 h, changes of BMP-7 protein levels measured by Western blotting. n = 3, # P

    Article Snippet: Antibodies against collagen-I (Cat#14695-1-AP), BMP-7 (Cat#12221-1-AP), α-smooth muscle actin (α-SMA) (Cat#55135-1-AP), TGF-β1 (Cat#21898-1-AP) and GAPDH (Cat#60004–1-lg) were purchased from Proteintech (Rosemont, IL, USA).

    Techniques: Recombinant, Plasmid Preparation, Negative Control, Western Blot

    miR-4739 is high but BMP-7 is low expressed in patients with pleural fibrosis. Pleural tissue samples were collected from parietal pleura in patients with TPE (TB1, 2, 3) or MPE (Tumor1, 2, 3). Pleura sections were stained with Masson's trichrome staining, in situ hybridization histochemistry and immunofluorescence staining. Blue color showed collagen in the Masson's trichrome stainings (a1-f1). In situ hybridization histochemistry stainings (a2-f2), yellow color showed miR-4739. Red color showed BMP-7 in immunofluorescence stainings (a3-f3). Green color showed calretinin in immunofluorescence staining (a4-f4). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: EBioMedicine

    Article Title: miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7

    doi: 10.1016/j.ebiom.2019.02.057

    Figure Lengend Snippet: miR-4739 is high but BMP-7 is low expressed in patients with pleural fibrosis. Pleural tissue samples were collected from parietal pleura in patients with TPE (TB1, 2, 3) or MPE (Tumor1, 2, 3). Pleura sections were stained with Masson's trichrome staining, in situ hybridization histochemistry and immunofluorescence staining. Blue color showed collagen in the Masson's trichrome stainings (a1-f1). In situ hybridization histochemistry stainings (a2-f2), yellow color showed miR-4739. Red color showed BMP-7 in immunofluorescence stainings (a3-f3). Green color showed calretinin in immunofluorescence staining (a4-f4). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Antibodies against collagen-I (Cat#14695-1-AP), BMP-7 (Cat#12221-1-AP), α-smooth muscle actin (α-SMA) (Cat#55135-1-AP), TGF-β1 (Cat#21898-1-AP) and GAPDH (Cat#60004–1-lg) were purchased from Proteintech (Rosemont, IL, USA).

    Techniques: Staining, In Situ Hybridization, Immunofluorescence

    miR-4739/BMP-7/Smad1/5/9 pathway induces pleural fibrosis. Bleomycin increased miR-4739 expression by activating Smad2/3 signaling. The up-expressed miR-4739 targeted and reduced BMP-7 expression which broke the balance between Smad1/5/9 and Smad2/3 signaling and led to pleural fibrosis.

    Journal: EBioMedicine

    Article Title: miR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7

    doi: 10.1016/j.ebiom.2019.02.057

    Figure Lengend Snippet: miR-4739/BMP-7/Smad1/5/9 pathway induces pleural fibrosis. Bleomycin increased miR-4739 expression by activating Smad2/3 signaling. The up-expressed miR-4739 targeted and reduced BMP-7 expression which broke the balance between Smad1/5/9 and Smad2/3 signaling and led to pleural fibrosis.

    Article Snippet: Antibodies against collagen-I (Cat#14695-1-AP), BMP-7 (Cat#12221-1-AP), α-smooth muscle actin (α-SMA) (Cat#55135-1-AP), TGF-β1 (Cat#21898-1-AP) and GAPDH (Cat#60004–1-lg) were purchased from Proteintech (Rosemont, IL, USA).

    Techniques: Expressing

    Systemic effects of BMP7 are leptin-independent. A , B ) Treatment of leptin-deficient ob/ob mice with BMP7 adenovirus resulted in significant weight loss ( A ) and reduced food consumption ( B ) compared to ob/ob mice that received LacZ adenovirus (control)

    Journal: The FASEB Journal

    Article Title: Bone morphogenetic protein 7 (BMP7) reverses obesity and regulates appetite through a central mTOR pathway

    doi: 10.1096/fj.11-199067

    Figure Lengend Snippet: Systemic effects of BMP7 are leptin-independent. A , B ) Treatment of leptin-deficient ob/ob mice with BMP7 adenovirus resulted in significant weight loss ( A ) and reduced food consumption ( B ) compared to ob/ob mice that received LacZ adenovirus (control)

    Article Snippet: At 1–2 h prior to the onset of the dark cycle, mice received an i.c.v. injection (1 μl) of BMP7 (2 μg/μl; R & D Systems, Minneapolis, MN, USA), leptin (1.6 μg/μl), or vehicle using an internal cannula connected to a Hamilton microsyringe (Hamilton, Reno, NV, USA).

    Techniques: Mouse Assay

    BMP7 utilizes the mTOR pathway to regulate food intake. A ) Western blot analysis demonstrating that BMP7, when injected i.c.v. in mice, activated multiple signaling pathways in the hypothalamus, most notably the p70S6K and pSTAT3 pathways. Cyclophilin

    Journal: The FASEB Journal

    Article Title: Bone morphogenetic protein 7 (BMP7) reverses obesity and regulates appetite through a central mTOR pathway

    doi: 10.1096/fj.11-199067

    Figure Lengend Snippet: BMP7 utilizes the mTOR pathway to regulate food intake. A ) Western blot analysis demonstrating that BMP7, when injected i.c.v. in mice, activated multiple signaling pathways in the hypothalamus, most notably the p70S6K and pSTAT3 pathways. Cyclophilin

    Article Snippet: At 1–2 h prior to the onset of the dark cycle, mice received an i.c.v. injection (1 μl) of BMP7 (2 μg/μl; R & D Systems, Minneapolis, MN, USA), leptin (1.6 μg/μl), or vehicle using an internal cannula connected to a Hamilton microsyringe (Hamilton, Reno, NV, USA).

    Techniques: Western Blot, Injection, Mouse Assay

    Systemic administration of BMP7 reduces appetite and body weight in DIO mice. A ) Body weight monitoring of DIO mice receiving BMP7 or LacZ adenovirus (control), showing a weight-lowering effect of BMP7. B ) DIO mice treated with BMP7 adenovirus had increased

    Journal: The FASEB Journal

    Article Title: Bone morphogenetic protein 7 (BMP7) reverses obesity and regulates appetite through a central mTOR pathway

    doi: 10.1096/fj.11-199067

    Figure Lengend Snippet: Systemic administration of BMP7 reduces appetite and body weight in DIO mice. A ) Body weight monitoring of DIO mice receiving BMP7 or LacZ adenovirus (control), showing a weight-lowering effect of BMP7. B ) DIO mice treated with BMP7 adenovirus had increased

    Article Snippet: At 1–2 h prior to the onset of the dark cycle, mice received an i.c.v. injection (1 μl) of BMP7 (2 μg/μl; R & D Systems, Minneapolis, MN, USA), leptin (1.6 μg/μl), or vehicle using an internal cannula connected to a Hamilton microsyringe (Hamilton, Reno, NV, USA).

    Techniques: Mouse Assay

    Expression of BMP7 ligand and receptors in the hypothalamus. A ) In situ hybridization analysis of hypothalamic sections for BMPRII (pink), showing caudal hypothalamus (left panel) and rostral hypothalamus (right panel). Antisense represents specific staining

    Journal: The FASEB Journal

    Article Title: Bone morphogenetic protein 7 (BMP7) reverses obesity and regulates appetite through a central mTOR pathway

    doi: 10.1096/fj.11-199067

    Figure Lengend Snippet: Expression of BMP7 ligand and receptors in the hypothalamus. A ) In situ hybridization analysis of hypothalamic sections for BMPRII (pink), showing caudal hypothalamus (left panel) and rostral hypothalamus (right panel). Antisense represents specific staining

    Article Snippet: At 1–2 h prior to the onset of the dark cycle, mice received an i.c.v. injection (1 μl) of BMP7 (2 μg/μl; R & D Systems, Minneapolis, MN, USA), leptin (1.6 μg/μl), or vehicle using an internal cannula connected to a Hamilton microsyringe (Hamilton, Reno, NV, USA).

    Techniques: Expressing, In Situ Hybridization, Staining

    Intracerebroventricular administration of BMP7 suppresses food intake. A ) Intracerebroventricular administration of rhBMP7 acutely reduced food intake in C57BL/6 mice. Results are presented from a representative of 7 independent experiments. Leptin served

    Journal: The FASEB Journal

    Article Title: Bone morphogenetic protein 7 (BMP7) reverses obesity and regulates appetite through a central mTOR pathway

    doi: 10.1096/fj.11-199067

    Figure Lengend Snippet: Intracerebroventricular administration of BMP7 suppresses food intake. A ) Intracerebroventricular administration of rhBMP7 acutely reduced food intake in C57BL/6 mice. Results are presented from a representative of 7 independent experiments. Leptin served

    Article Snippet: At 1–2 h prior to the onset of the dark cycle, mice received an i.c.v. injection (1 μl) of BMP7 (2 μg/μl; R & D Systems, Minneapolis, MN, USA), leptin (1.6 μg/μl), or vehicle using an internal cannula connected to a Hamilton microsyringe (Hamilton, Reno, NV, USA).

    Techniques: Mouse Assay

    Downstream BMP7 signaling in PCC cells A. PC12 cells were transfected with a BMP7-containing (BMP7) or an empty (mock) vector. Twenty-four h post transfection IF was performed using specific antibodies targeting integrin β1 (1:400) or BMP7 (1:100). Cell nuclei were counterstained with DAPI. In parallel, the expression of integrin β1 and BMP7 proteins was determined by western blotting using specific antibodies. α-Tubulin was used as loading control. B. PC12 cells were either transfected (txBMP7) with a Myc-BMP7 plasmid or treated with 100 ng/mL recombinant human BMP7 (rhBMP7). After 24 h, cells were collected and analyzed by western blotting using antibodies against P-Smad1/5/8 and Smad1. α-Tubulin was used as a loading control. C. PC12 cells were treated with rhBMP7 for 5, 15, 30, 45, or 60 min. Western blot analysis was performed using specific antibodies raised against integrin β1, AKT and P-AKT. α-Tubulin was used as loading control. D. We co-transfected PC12 cells with BMP7 and scr-siRNA or siRNA- Itgb1 and 24 h later proliferation, migration and invasion were assessed. Values for cells with knockdown of Itgb1 were normalized against the values of scr-siRNA-transfected cells arbitrarily set to 100%. The experiments were performed two times with three technical replicates with similar results. For proliferation, the average of the 2 experiments is shown. For migration/invasion, five random fields of each test at × 400 magnification were counted (average ± SD). **, P

    Journal: Oncotarget

    Article Title: Oncogenic features of the bone morphogenic protein 7 (BMP7) in pheochromocytoma

    doi:

    Figure Lengend Snippet: Downstream BMP7 signaling in PCC cells A. PC12 cells were transfected with a BMP7-containing (BMP7) or an empty (mock) vector. Twenty-four h post transfection IF was performed using specific antibodies targeting integrin β1 (1:400) or BMP7 (1:100). Cell nuclei were counterstained with DAPI. In parallel, the expression of integrin β1 and BMP7 proteins was determined by western blotting using specific antibodies. α-Tubulin was used as loading control. B. PC12 cells were either transfected (txBMP7) with a Myc-BMP7 plasmid or treated with 100 ng/mL recombinant human BMP7 (rhBMP7). After 24 h, cells were collected and analyzed by western blotting using antibodies against P-Smad1/5/8 and Smad1. α-Tubulin was used as a loading control. C. PC12 cells were treated with rhBMP7 for 5, 15, 30, 45, or 60 min. Western blot analysis was performed using specific antibodies raised against integrin β1, AKT and P-AKT. α-Tubulin was used as loading control. D. We co-transfected PC12 cells with BMP7 and scr-siRNA or siRNA- Itgb1 and 24 h later proliferation, migration and invasion were assessed. Values for cells with knockdown of Itgb1 were normalized against the values of scr-siRNA-transfected cells arbitrarily set to 100%. The experiments were performed two times with three technical replicates with similar results. For proliferation, the average of the 2 experiments is shown. For migration/invasion, five random fields of each test at × 400 magnification were counted (average ± SD). **, P

    Article Snippet: The primary antibodies used are: human anti-BMP7 (R & D Systems, USA; clone #164311; dilution 1:1000); rat anti-Bmp7 (#4693, 1:100), P-Smad1/5/8 (#9511, 1:50), Smad1 (#5753; 1:500), AKT (#9272; 1:500) and P-AKT (#9271; 1:500) all from Cell Signaling Technology (Danvers MA, USA); integrin β1 (Abcam, Cambridge, UK; #EP1041Y; dilution 1:500); anti-Myc tag (Clontech, St-Germain-en-Laye, France; #631206; dilution 1:500), α-Tubulin (Sigma-Aldrich, Hamburg, Germany; #T5168; dilution 1:1000).

    Techniques: Periodic Counter-current Chromatography, Transfection, Plasmid Preparation, Expressing, Western Blot, Recombinant, Migration

    Expression of BMP7 in human PCC A–D. IHC for BMP7 was performed on tissue microarrays using a human anti-BMP7 antibody. After selection, 150 PCCs and 34 PGLs were scored. We only considered tumors for which both cores could be scored after IHC staining. Images were recorded and we show low (original magnification, × 40) and high (original magnification, × 400) power views of four different PCCs representative of the intensities of BMP7 staining (A,−; B,+; C,++; D,+++). E–H. BMP7 expression of the 184 tumors was correlated with clinical variables, including (E) location (Extra-adrenal PCC = PGL; Adrenal PCC = Pheo), (F) tumor size (

    Journal: Oncotarget

    Article Title: Oncogenic features of the bone morphogenic protein 7 (BMP7) in pheochromocytoma

    doi:

    Figure Lengend Snippet: Expression of BMP7 in human PCC A–D. IHC for BMP7 was performed on tissue microarrays using a human anti-BMP7 antibody. After selection, 150 PCCs and 34 PGLs were scored. We only considered tumors for which both cores could be scored after IHC staining. Images were recorded and we show low (original magnification, × 40) and high (original magnification, × 400) power views of four different PCCs representative of the intensities of BMP7 staining (A,−; B,+; C,++; D,+++). E–H. BMP7 expression of the 184 tumors was correlated with clinical variables, including (E) location (Extra-adrenal PCC = PGL; Adrenal PCC = Pheo), (F) tumor size (

    Article Snippet: The primary antibodies used are: human anti-BMP7 (R & D Systems, USA; clone #164311; dilution 1:1000); rat anti-Bmp7 (#4693, 1:100), P-Smad1/5/8 (#9511, 1:50), Smad1 (#5753; 1:500), AKT (#9272; 1:500) and P-AKT (#9271; 1:500) all from Cell Signaling Technology (Danvers MA, USA); integrin β1 (Abcam, Cambridge, UK; #EP1041Y; dilution 1:500); anti-Myc tag (Clontech, St-Germain-en-Laye, France; #631206; dilution 1:500), α-Tubulin (Sigma-Aldrich, Hamburg, Germany; #T5168; dilution 1:1000).

    Techniques: Expressing, Periodic Counter-current Chromatography, Immunohistochemistry, Selection, Staining

    Bmp7 promotes proliferation of PCC cells in vitro A. We transfected PC12 cells with a Myc-BMP7 plasmid, and 24 h later cells were analyzed by western blotting using a specific anti-Myc antibody. B. MPC, C. MTT and D. primary rat tumor (P.C.) cells were infected with lentiviral vectors containing shBMP7-GFP (#2.9) or GFP alone. Western blotting was performed 72 h later using the rat anti-BMP7 antibody. α-Tubulin was used as loading control. E. PC12 cells were transfected with the BMP7 plasmid (BMP7) or with the mock vector (Mock) and proliferation was assessed 24 h later using the WST-1 assay. Data were analyzed independently with six technical replicates each, and are expressed as the mean ± SD. Proliferation was normalized against the values of mock transfected cells arbitrarily set to 100%. **, P

    Journal: Oncotarget

    Article Title: Oncogenic features of the bone morphogenic protein 7 (BMP7) in pheochromocytoma

    doi:

    Figure Lengend Snippet: Bmp7 promotes proliferation of PCC cells in vitro A. We transfected PC12 cells with a Myc-BMP7 plasmid, and 24 h later cells were analyzed by western blotting using a specific anti-Myc antibody. B. MPC, C. MTT and D. primary rat tumor (P.C.) cells were infected with lentiviral vectors containing shBMP7-GFP (#2.9) or GFP alone. Western blotting was performed 72 h later using the rat anti-BMP7 antibody. α-Tubulin was used as loading control. E. PC12 cells were transfected with the BMP7 plasmid (BMP7) or with the mock vector (Mock) and proliferation was assessed 24 h later using the WST-1 assay. Data were analyzed independently with six technical replicates each, and are expressed as the mean ± SD. Proliferation was normalized against the values of mock transfected cells arbitrarily set to 100%. **, P

    Article Snippet: The primary antibodies used are: human anti-BMP7 (R & D Systems, USA; clone #164311; dilution 1:1000); rat anti-Bmp7 (#4693, 1:100), P-Smad1/5/8 (#9511, 1:50), Smad1 (#5753; 1:500), AKT (#9272; 1:500) and P-AKT (#9271; 1:500) all from Cell Signaling Technology (Danvers MA, USA); integrin β1 (Abcam, Cambridge, UK; #EP1041Y; dilution 1:500); anti-Myc tag (Clontech, St-Germain-en-Laye, France; #631206; dilution 1:500), α-Tubulin (Sigma-Aldrich, Hamburg, Germany; #T5168; dilution 1:1000).

    Techniques: Periodic Counter-current Chromatography, In Vitro, Transfection, Plasmid Preparation, Western Blot, MTT Assay, Infection, WST-1 Assay

    Bmp7 enhances migration and invasion of PCC cells in vitro We transfected PC12 cells with a Myc-BMP7 plasmid and 24 h later A, C. migration and B, E. invasion were assessed. MPC and MTT cells were infected with lentiviral vectors containing sh Bmp7 -GFP or GFP alone, and 72 h later we assessed A, D. migration and B, F. invasion. The percentage of cells that migrated or invaded was normalized against the values of mock transfected cells or of GFP-infected cells arbitrarily set to 100%. The experiment was performed two times with three technical replicates with similar results. Five random fields of each test at ×400 magnification were counted (±SD). **, P

    Journal: Oncotarget

    Article Title: Oncogenic features of the bone morphogenic protein 7 (BMP7) in pheochromocytoma

    doi:

    Figure Lengend Snippet: Bmp7 enhances migration and invasion of PCC cells in vitro We transfected PC12 cells with a Myc-BMP7 plasmid and 24 h later A, C. migration and B, E. invasion were assessed. MPC and MTT cells were infected with lentiviral vectors containing sh Bmp7 -GFP or GFP alone, and 72 h later we assessed A, D. migration and B, F. invasion. The percentage of cells that migrated or invaded was normalized against the values of mock transfected cells or of GFP-infected cells arbitrarily set to 100%. The experiment was performed two times with three technical replicates with similar results. Five random fields of each test at ×400 magnification were counted (±SD). **, P

    Article Snippet: The primary antibodies used are: human anti-BMP7 (R & D Systems, USA; clone #164311; dilution 1:1000); rat anti-Bmp7 (#4693, 1:100), P-Smad1/5/8 (#9511, 1:50), Smad1 (#5753; 1:500), AKT (#9272; 1:500) and P-AKT (#9271; 1:500) all from Cell Signaling Technology (Danvers MA, USA); integrin β1 (Abcam, Cambridge, UK; #EP1041Y; dilution 1:500); anti-Myc tag (Clontech, St-Germain-en-Laye, France; #631206; dilution 1:500), α-Tubulin (Sigma-Aldrich, Hamburg, Germany; #T5168; dilution 1:1000).

    Techniques: Migration, Periodic Counter-current Chromatography, In Vitro, Transfection, Plasmid Preparation, MTT Assay, Infection

    Expression of Bmp7 in rat PCC and its secretion A. IHC or IF on adrenal medullary tissue from wild-type and mutant rats was performed using a rat anti-BMP7 antibody or an anti-P-Smad1/5/8 antibody, respectively. These tissues are representative of five tissues per animal group. Original magnification: 400× B. Plasma Bmp7 levels were measured in seven MENX mutant rats and seven wild-type rats fasted for 12 h. Measurements were performed using a rat Bmp7 ELISA-KIT. ***, P

    Journal: Oncotarget

    Article Title: Oncogenic features of the bone morphogenic protein 7 (BMP7) in pheochromocytoma

    doi:

    Figure Lengend Snippet: Expression of Bmp7 in rat PCC and its secretion A. IHC or IF on adrenal medullary tissue from wild-type and mutant rats was performed using a rat anti-BMP7 antibody or an anti-P-Smad1/5/8 antibody, respectively. These tissues are representative of five tissues per animal group. Original magnification: 400× B. Plasma Bmp7 levels were measured in seven MENX mutant rats and seven wild-type rats fasted for 12 h. Measurements were performed using a rat Bmp7 ELISA-KIT. ***, P

    Article Snippet: The primary antibodies used are: human anti-BMP7 (R & D Systems, USA; clone #164311; dilution 1:1000); rat anti-Bmp7 (#4693, 1:100), P-Smad1/5/8 (#9511, 1:50), Smad1 (#5753; 1:500), AKT (#9272; 1:500) and P-AKT (#9271; 1:500) all from Cell Signaling Technology (Danvers MA, USA); integrin β1 (Abcam, Cambridge, UK; #EP1041Y; dilution 1:500); anti-Myc tag (Clontech, St-Germain-en-Laye, France; #631206; dilution 1:500), α-Tubulin (Sigma-Aldrich, Hamburg, Germany; #T5168; dilution 1:1000).

    Techniques: Expressing, Periodic Counter-current Chromatography, Immunohistochemistry, Mutagenesis, Enzyme-linked Immunosorbent Assay

    Known regulators of hepcidin have a modest effect on regulation of hepcidin in breast cancer spheroids (A) RT-qPCR of Hepcidin mRNA (normalized to Cyclophilin A) and (B) western blot analysis of pro-hepcidin and β-actin following siRNA knock-down of non-target control (NTC), BMP4, BMP6, and BMP7 in MCF-7 spheroids. Untreated MCF-7 cells grown in 2D (A) or 3D (B) were used as controls. Statistical analysis and quantification was normalized to non-targeting control siRNA. (C) Western blot analysis of p-STAT3, total STAT-3 and Cyclophilin B in MCF-7 monolayer versus spheroids cultured for 3 days. (D) RT-qPCR of hepcidin mRNA (normalized to Cyclophilin A) following siRNA knock-down of NTC and STAT3 in MCF-7 spheroids. For statistical analysis samples were compared to non-targeting control. Untreated MCF-7 2D was used as a control.

    Journal: Oncogene

    Article Title: Contribution of three dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer

    doi: 10.1038/s41388-018-0243-y

    Figure Lengend Snippet: Known regulators of hepcidin have a modest effect on regulation of hepcidin in breast cancer spheroids (A) RT-qPCR of Hepcidin mRNA (normalized to Cyclophilin A) and (B) western blot analysis of pro-hepcidin and β-actin following siRNA knock-down of non-target control (NTC), BMP4, BMP6, and BMP7 in MCF-7 spheroids. Untreated MCF-7 cells grown in 2D (A) or 3D (B) were used as controls. Statistical analysis and quantification was normalized to non-targeting control siRNA. (C) Western blot analysis of p-STAT3, total STAT-3 and Cyclophilin B in MCF-7 monolayer versus spheroids cultured for 3 days. (D) RT-qPCR of hepcidin mRNA (normalized to Cyclophilin A) following siRNA knock-down of NTC and STAT3 in MCF-7 spheroids. For statistical analysis samples were compared to non-targeting control. Untreated MCF-7 2D was used as a control.

    Article Snippet: Neutralizing antibody and recombinant protein treatments For neutralization of BMPs, cells were treated with 1 or 3 μg/mL anti-BMP4, anti-BMP6, anti-BMP7, (R & D Systems, Minneapolis, MN, USA cat#MAB757, MAB507, MAB3541) or 3 μg/mL isotope-matched anti-IgG (R & D systems, cat#MAB004) for 48 hours.

    Techniques: Quantitative RT-PCR, Western Blot, Cell Culture

    BMPs regulate hepcidin expression in MCF-7 breast cancer cells (A) RT-qPCR of BMP4, BMP6 or BMP7 mRNA (normalized to β-actin) in MCF-7 and MCF-10A monolayer cells. (B) Western blot analysis of pro-hepcidin and β-actin following siRNA knock-down of non-target control (NTC), BMP4, BMP6, and BMP7 for 48 hours in MCF-7 cells. For quantification, samples were compared to NTC. GAPDH siRNA was used as an additional control. (C) Western blot analysis of pro-hepcidin and β-actin after the addition of 1 and 3 μg/mL neutralizing antibodies against BMP4, BMP6, BMP7 or IgG (3μg/ml) isotope control for 48 hrs in MCF-7 cells. For quantification, samples were compared to untreated sample. (D) Western blot analysis of phosporylated-STAT3 (pSTAT3), total STAT3 (tSTAT3), pro-hepcidin, and β-actin following the addition of recombinant IL-6 for 24 hours in MCF-7 cells.

    Journal: Oncogene

    Article Title: Contribution of three dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer

    doi: 10.1038/s41388-018-0243-y

    Figure Lengend Snippet: BMPs regulate hepcidin expression in MCF-7 breast cancer cells (A) RT-qPCR of BMP4, BMP6 or BMP7 mRNA (normalized to β-actin) in MCF-7 and MCF-10A monolayer cells. (B) Western blot analysis of pro-hepcidin and β-actin following siRNA knock-down of non-target control (NTC), BMP4, BMP6, and BMP7 for 48 hours in MCF-7 cells. For quantification, samples were compared to NTC. GAPDH siRNA was used as an additional control. (C) Western blot analysis of pro-hepcidin and β-actin after the addition of 1 and 3 μg/mL neutralizing antibodies against BMP4, BMP6, BMP7 or IgG (3μg/ml) isotope control for 48 hrs in MCF-7 cells. For quantification, samples were compared to untreated sample. (D) Western blot analysis of phosporylated-STAT3 (pSTAT3), total STAT3 (tSTAT3), pro-hepcidin, and β-actin following the addition of recombinant IL-6 for 24 hours in MCF-7 cells.

    Article Snippet: Neutralizing antibody and recombinant protein treatments For neutralization of BMPs, cells were treated with 1 or 3 μg/mL anti-BMP4, anti-BMP6, anti-BMP7, (R & D Systems, Minneapolis, MN, USA cat#MAB757, MAB507, MAB3541) or 3 μg/mL isotope-matched anti-IgG (R & D systems, cat#MAB004) for 48 hours.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Recombinant

    BMP7 bioactivity was not affected in patient variants. The ability of the BMP7 variants to induce pSMAD 1/5 activation was assessed by (A) a luciferase bioassay and (B) analysis of endogenous pSMAD 1/5 levels, both in COV434 cells.

    Journal: Journal of the Endocrine Society

    Article Title: Functional Characterization of Two New Variants in the Bone Morphogenetic Protein 7 Prodomain in Two Pairs of Monozygotic Twins With Hypospadias

    doi: 10.1210/js.2018-00333

    Figure Lengend Snippet: BMP7 bioactivity was not affected in patient variants. The ability of the BMP7 variants to induce pSMAD 1/5 activation was assessed by (A) a luciferase bioassay and (B) analysis of endogenous pSMAD 1/5 levels, both in COV434 cells.

    Article Snippet: Blots were blocked in 1% BSA in TBS-Tween buffer (Tris-buffered saline with 0.05% Tween-20) for a minimum of one hour, and then probed with a BMP7 antibody (MAB3542, R & D Systems, Minneapolis, MN) diluted in TBS-Tw buffer (1:5000) overnight.

    Techniques: Activation Assay, Luciferase

    BMP7 variants affect protein expression. (A) Homozygous and heterozygous variants’ expression is compared by Western Blot to the wild-type and standard BMP7 (Std). (B) Densitometry results for the two variants, at both homozygous and heterozygous states. Twin pair 1: G265T (A89S). Twin pair 2: G634A (D212N). (C) Total protein concentrations as determined by a BCA assay.

    Journal: Journal of the Endocrine Society

    Article Title: Functional Characterization of Two New Variants in the Bone Morphogenetic Protein 7 Prodomain in Two Pairs of Monozygotic Twins With Hypospadias

    doi: 10.1210/js.2018-00333

    Figure Lengend Snippet: BMP7 variants affect protein expression. (A) Homozygous and heterozygous variants’ expression is compared by Western Blot to the wild-type and standard BMP7 (Std). (B) Densitometry results for the two variants, at both homozygous and heterozygous states. Twin pair 1: G265T (A89S). Twin pair 2: G634A (D212N). (C) Total protein concentrations as determined by a BCA assay.

    Article Snippet: Blots were blocked in 1% BSA in TBS-Tween buffer (Tris-buffered saline with 0.05% Tween-20) for a minimum of one hour, and then probed with a BMP7 antibody (MAB3542, R & D Systems, Minneapolis, MN) diluted in TBS-Tw buffer (1:5000) overnight.

    Techniques: Expressing, Western Blot, BIA-KA

    Variants affect highly conserved amino-acids in the BMP7 prodomain. (A) Variants in both pairs of twins (nucleic acid highlighted in orange) affect amino acids highly conserved across multiple species. (B) Sanger sequencing confirmation for both variants. Pair 1: exon 1. c.G265T; p.A89S. Pair 2: exon 3 c.G634A; p.D212N.

    Journal: Journal of the Endocrine Society

    Article Title: Functional Characterization of Two New Variants in the Bone Morphogenetic Protein 7 Prodomain in Two Pairs of Monozygotic Twins With Hypospadias

    doi: 10.1210/js.2018-00333

    Figure Lengend Snippet: Variants affect highly conserved amino-acids in the BMP7 prodomain. (A) Variants in both pairs of twins (nucleic acid highlighted in orange) affect amino acids highly conserved across multiple species. (B) Sanger sequencing confirmation for both variants. Pair 1: exon 1. c.G265T; p.A89S. Pair 2: exon 3 c.G634A; p.D212N.

    Article Snippet: Blots were blocked in 1% BSA in TBS-Tween buffer (Tris-buffered saline with 0.05% Tween-20) for a minimum of one hour, and then probed with a BMP7 antibody (MAB3542, R & D Systems, Minneapolis, MN) diluted in TBS-Tw buffer (1:5000) overnight.

    Techniques: Sequencing

    Experimental workflow and numbers of differentially expressed probes (DEPs) resulting from BMP4 or BMP7 treatment . (A) Seven breast cancer cell lines were cultured on 24-well plates, allowed to adhere for 24 h, and treated with the BMP ligand or vehicle for 30 min, 1 h, 3 h, 6 h, 12 h, and 24 h. Experiments were performed in triplicate, and collected cells were pooled. (B) The expression data from each cell line were individually filtered according to the following criteria: differential expression of at least 2-fold at a minimum of one time point. The number of DEPs per cell line is represented. (C) The expression data from individual time points of every cell line were filtered according to a 2-fold cutoff in expression change. The number of DEPs per time point is shown.

    Journal: BMC Medical Genomics

    Article Title: Analysis of BMP4 and BMP7 signaling in breast cancer cells unveils time-dependent transcription patterns and highlights a common synexpression group of genes

    doi: 10.1186/1755-8794-4-80

    Figure Lengend Snippet: Experimental workflow and numbers of differentially expressed probes (DEPs) resulting from BMP4 or BMP7 treatment . (A) Seven breast cancer cell lines were cultured on 24-well plates, allowed to adhere for 24 h, and treated with the BMP ligand or vehicle for 30 min, 1 h, 3 h, 6 h, 12 h, and 24 h. Experiments were performed in triplicate, and collected cells were pooled. (B) The expression data from each cell line were individually filtered according to the following criteria: differential expression of at least 2-fold at a minimum of one time point. The number of DEPs per cell line is represented. (C) The expression data from individual time points of every cell line were filtered according to a 2-fold cutoff in expression change. The number of DEPs per time point is shown.

    Article Snippet: BMP4 and BMP7 treatments Recombinant human BMP4 and BMP7 proteins were purchased from R & D Systems (Minneapolis, MN).

    Techniques: Cell Culture, Expressing

    Time series analyses . Representative clusters from the four temporal categories are shown for BMP4 (A) and BMP7 (B). For each cluster, the upper figure shows the levels of differential expression through the time series for every probe. The lower chart represents the average value of differential expression for all the probes in the cluster along the time scale. The number of probes in each cluster is indicated under the cell line name. (C) GO analyses of the four temporal categories were performed, and data from the five cell lines were combined. The enriched GO terms for BMP4 are depicted.

    Journal: BMC Medical Genomics

    Article Title: Analysis of BMP4 and BMP7 signaling in breast cancer cells unveils time-dependent transcription patterns and highlights a common synexpression group of genes

    doi: 10.1186/1755-8794-4-80

    Figure Lengend Snippet: Time series analyses . Representative clusters from the four temporal categories are shown for BMP4 (A) and BMP7 (B). For each cluster, the upper figure shows the levels of differential expression through the time series for every probe. The lower chart represents the average value of differential expression for all the probes in the cluster along the time scale. The number of probes in each cluster is indicated under the cell line name. (C) GO analyses of the four temporal categories were performed, and data from the five cell lines were combined. The enriched GO terms for BMP4 are depicted.

    Article Snippet: BMP4 and BMP7 treatments Recombinant human BMP4 and BMP7 proteins were purchased from R & D Systems (Minneapolis, MN).

    Techniques: Expressing

    Expression of BMP7 in MCL lymphoma cells. Figure 2A: from patients tested by DNA chips, RT-PCR was performed in two independent experiments before therapy (lane 1, 3, 5, 7 and 9) and after therapy (lane 2, 4, 6, 8 and 10). Patient 1 (lanes 1 and 2), patient 2 (lanes 3 and 4), patient 3 (lanes 5 and 6), patient 4 (lane7 and 8) patient 5 (lanes 9 and 10). Patients 1, 2 and 4 were initially sensitive to chemotherapy (secondary resistant tumors). Human placenta was used as a positive control (lane 11) and mix without cDNA as negative control (lane 12). BMP7 was expressed in the 3 patients at relapse, but was not expressed in the 2 patients with refractory disease at diagnosis and after having failed on therapy. ß2microglobuline probe was used as internal reference. Figure 2B: Immunohistochemical analysis of one of the 2 patient’s tumor at diagnosis and at relapse, that expressed BMP7 at relapse (secondary resistant lymphoma).

    Journal: PLoS ONE

    Article Title: BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma

    doi: 10.1371/journal.pone.0073993

    Figure Lengend Snippet: Expression of BMP7 in MCL lymphoma cells. Figure 2A: from patients tested by DNA chips, RT-PCR was performed in two independent experiments before therapy (lane 1, 3, 5, 7 and 9) and after therapy (lane 2, 4, 6, 8 and 10). Patient 1 (lanes 1 and 2), patient 2 (lanes 3 and 4), patient 3 (lanes 5 and 6), patient 4 (lane7 and 8) patient 5 (lanes 9 and 10). Patients 1, 2 and 4 were initially sensitive to chemotherapy (secondary resistant tumors). Human placenta was used as a positive control (lane 11) and mix without cDNA as negative control (lane 12). BMP7 was expressed in the 3 patients at relapse, but was not expressed in the 2 patients with refractory disease at diagnosis and after having failed on therapy. ß2microglobuline probe was used as internal reference. Figure 2B: Immunohistochemical analysis of one of the 2 patient’s tumor at diagnosis and at relapse, that expressed BMP7 at relapse (secondary resistant lymphoma).

    Article Snippet: The mouse monoclonal antibody directed against human BMP7 (R & D Systems, Inc., Minneapolis, MN ; Ref : MAB3542 ; clone 164313) was used at 25 µg/mL (1:40).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Negative Control, Immunohistochemistry

    SiRNA BMP7 had a negative effect on cell survival at all time points assessed with both drugs showing that BMP7 plays a role in drug resistance. Jeko cells were exposed with Bortezomib (5 ng/ml) or Cytarabine (20 µg/ml) 24h or 48h after transfection with a BMP7 siRNA or a nonsilencing siRNA used as a control (see material and methods). BMP7 protein expression was assessed by immunoblotting analyzes. Annexin V- and PI-negative cells (non apoptotic) were quantified 48h and 72h after transfection. BMP7 siRNA markedly increased the fraction of annexin V- and PI-negative cells showing that BMP7 suppression plays a role in Jeko cell chemosensitivity to Bortezomib and Cytarabine.

    Journal: PLoS ONE

    Article Title: BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma

    doi: 10.1371/journal.pone.0073993

    Figure Lengend Snippet: SiRNA BMP7 had a negative effect on cell survival at all time points assessed with both drugs showing that BMP7 plays a role in drug resistance. Jeko cells were exposed with Bortezomib (5 ng/ml) or Cytarabine (20 µg/ml) 24h or 48h after transfection with a BMP7 siRNA or a nonsilencing siRNA used as a control (see material and methods). BMP7 protein expression was assessed by immunoblotting analyzes. Annexin V- and PI-negative cells (non apoptotic) were quantified 48h and 72h after transfection. BMP7 siRNA markedly increased the fraction of annexin V- and PI-negative cells showing that BMP7 suppression plays a role in Jeko cell chemosensitivity to Bortezomib and Cytarabine.

    Article Snippet: The mouse monoclonal antibody directed against human BMP7 (R & D Systems, Inc., Minneapolis, MN ; Ref : MAB3542 ; clone 164313) was used at 25 µg/mL (1:40).

    Techniques: Transfection, Expressing

    A: Expression of mRNAs encoding BMP7 tested by RT-PCR performed on 1: UPN1, 2: Jeko, 3: Rec-1, 4: GRANTA-519 and 5: placenta (positive control). B: methylation profile of BMP-7 CpG islands in the cell line C: UPN1, Jeko, Rec-1 and GRANTA -519 cells were cultured in serum-free media in the presence or absence of 200 ng/ml of BMP-7 for 7 days. BMP-7 had no effect on Jeko, GRANTA -519 and Rec-1 cell survival and on annexin V-positive cells compared to serum-free media alone.

    Journal: PLoS ONE

    Article Title: BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma

    doi: 10.1371/journal.pone.0073993

    Figure Lengend Snippet: A: Expression of mRNAs encoding BMP7 tested by RT-PCR performed on 1: UPN1, 2: Jeko, 3: Rec-1, 4: GRANTA-519 and 5: placenta (positive control). B: methylation profile of BMP-7 CpG islands in the cell line C: UPN1, Jeko, Rec-1 and GRANTA -519 cells were cultured in serum-free media in the presence or absence of 200 ng/ml of BMP-7 for 7 days. BMP-7 had no effect on Jeko, GRANTA -519 and Rec-1 cell survival and on annexin V-positive cells compared to serum-free media alone.

    Article Snippet: The mouse monoclonal antibody directed against human BMP7 (R & D Systems, Inc., Minneapolis, MN ; Ref : MAB3542 ; clone 164313) was used at 25 µg/mL (1:40).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control, Methylation, Cell Culture

    Gene expression ratios were calculated for each gene in primary refractory (non-responders) and in responders (secondary resistant). Gene expression ratio is equal to the geometric mean gene expression after treatment divided by the geometric mean gene expression before treatment. Gene expression ratios greater than one correspond to increased expression after treatment and conversely. Each point on the figure corresponds to a gene expression ratio in responders according to the expression ratio in non-responders. Nine genes classified as relevant (see methods) are symbolized by black dots. BMP7 which was selected for further investigations is symbolized by a red dot. Small grey symbols were used for “Other genes”.

    Journal: PLoS ONE

    Article Title: BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma

    doi: 10.1371/journal.pone.0073993

    Figure Lengend Snippet: Gene expression ratios were calculated for each gene in primary refractory (non-responders) and in responders (secondary resistant). Gene expression ratio is equal to the geometric mean gene expression after treatment divided by the geometric mean gene expression before treatment. Gene expression ratios greater than one correspond to increased expression after treatment and conversely. Each point on the figure corresponds to a gene expression ratio in responders according to the expression ratio in non-responders. Nine genes classified as relevant (see methods) are symbolized by black dots. BMP7 which was selected for further investigations is symbolized by a red dot. Small grey symbols were used for “Other genes”.

    Article Snippet: The mouse monoclonal antibody directed against human BMP7 (R & D Systems, Inc., Minneapolis, MN ; Ref : MAB3542 ; clone 164313) was used at 25 µg/mL (1:40).

    Techniques: Expressing

    Perioperative X-rays of a femoral non-union. Notes: Before non-union treatment according to the diamond concept ( A ) and 6 months postoperatively when consolidation was achieved ( B ). The intramedullary nail was removed, the non-union resected and the gap was filled with ABG (autologous bone graft), 3.3 mg BMP-7 and a tricalcium phosphate bone graft (Vitoss; Stryker, Duisburg, Germany). Re-osteosynthesis was performed with an angled blade plate.

    Journal: Therapeutics and Clinical Risk Management

    Article Title: Safety study: is there a pathologic IGF-1, PDGF and TGF-β cytokine expression caused by adjunct BMP-7 in tibial and femoral non-union therapy?

    doi: 10.2147/TCRM.S160064

    Figure Lengend Snippet: Perioperative X-rays of a femoral non-union. Notes: Before non-union treatment according to the diamond concept ( A ) and 6 months postoperatively when consolidation was achieved ( B ). The intramedullary nail was removed, the non-union resected and the gap was filled with ABG (autologous bone graft), 3.3 mg BMP-7 and a tricalcium phosphate bone graft (Vitoss; Stryker, Duisburg, Germany). Re-osteosynthesis was performed with an angled blade plate.

    Article Snippet: In addition to that, the results of our previous studies showed that local application of BMP-7 leads to a cytokine expression pattern similar to that of patients with proper bone healing., Since the application of both BMP-7 and ABG is helpful for bone healing, a combined treatment is successfully used for the therapy of atrophic non-unions., Yet, the safety of additional BMP-7 administration has never been assured on a molecular level comparing the cytokine expressions to an equivalent group that has received ABG alone.

    Techniques:

    Groups of matched patients: G1 – treatment with BMP-7 and autologous bone graft (ABG) according to the diamond concept, G2 – treatment with ABG alone.

    Journal: Therapeutics and Clinical Risk Management

    Article Title: Safety study: is there a pathologic IGF-1, PDGF and TGF-β cytokine expression caused by adjunct BMP-7 in tibial and femoral non-union therapy?

    doi: 10.2147/TCRM.S160064

    Figure Lengend Snippet: Groups of matched patients: G1 – treatment with BMP-7 and autologous bone graft (ABG) according to the diamond concept, G2 – treatment with ABG alone.

    Article Snippet: In addition to that, the results of our previous studies showed that local application of BMP-7 leads to a cytokine expression pattern similar to that of patients with proper bone healing., Since the application of both BMP-7 and ABG is helpful for bone healing, a combined treatment is successfully used for the therapy of atrophic non-unions., Yet, the safety of additional BMP-7 administration has never been assured on a molecular level comparing the cytokine expressions to an equivalent group that has received ABG alone.

    Techniques:

    Analysis of sequential IGF-1 ( A ), PDGF-AB ( B ) and TGF-β ( C ) expression levels of G1 (autologous bone graft with additional BMP-7) and G2 (autologous bone graft alone) groups expressed as absolute mean concentrations ± standard deviation. Notes: Serum concentration was measured in picograms per milliliter (pg/mL). The Wilcoxon’s signed-rank test assessed significant differences between both groups at each particular time point (*indicates significant differences, p

    Journal: Therapeutics and Clinical Risk Management

    Article Title: Safety study: is there a pathologic IGF-1, PDGF and TGF-β cytokine expression caused by adjunct BMP-7 in tibial and femoral non-union therapy?

    doi: 10.2147/TCRM.S160064

    Figure Lengend Snippet: Analysis of sequential IGF-1 ( A ), PDGF-AB ( B ) and TGF-β ( C ) expression levels of G1 (autologous bone graft with additional BMP-7) and G2 (autologous bone graft alone) groups expressed as absolute mean concentrations ± standard deviation. Notes: Serum concentration was measured in picograms per milliliter (pg/mL). The Wilcoxon’s signed-rank test assessed significant differences between both groups at each particular time point (*indicates significant differences, p

    Article Snippet: In addition to that, the results of our previous studies showed that local application of BMP-7 leads to a cytokine expression pattern similar to that of patients with proper bone healing., Since the application of both BMP-7 and ABG is helpful for bone healing, a combined treatment is successfully used for the therapy of atrophic non-unions., Yet, the safety of additional BMP-7 administration has never been assured on a molecular level comparing the cytokine expressions to an equivalent group that has received ABG alone.

    Techniques: Expressing, Standard Deviation, Concentration Assay

    Immunofluorescence and functional in vitro analysis of BMP-7–treated hNEPT. A , top row: BMP-7–induced cell aggregates coexpress insulin (green) and C-PEP (C-peptide, red). Channel merge and DAPI nuclear staining (blue) are shown at the right. Bottom row: Nuclear PDX-1 (red)/cytoplasmic insulin (green). Channel merge and nuclear staining (blue) are shown at the right. Scale bar, 50 μm. B , top row, from left to right: INS (insulin, green)/GLU (glucagon, red); INS (insulin, green)/PPY (red); INS (insulin, green)/SST (red). Bottom row, from left to right: INS (insulin, green)/NKX6.1 (red); INS (green)/MAFA (red); and INS (green)/PDX-1 (red)/DAPI (blue) in a representative insulin − /PDX-1 + cluster. Scale bars, 50 μm for top row and pictures 1 and 2 on the bottom row. Picture 3, bottom row: 100 μm. C : GSIS of control and treated (BMP-7) hNEPT ( n = 5 preparations). x -axis: L1, low glucose 1 (2.5 mmol/L); H, high glucose (20 mmol/L); L2, low glucose 2 (2.5 mmol/L). y -axis: human C-peptide (ng/μg of DNA). A second exposure to low glucose was performed to rule out false-positive results caused by “dumping” (the nonphysiological release of insulin). Data are presented as the mean ± SD ( n = 5). n.s., no significance ( P > 0.05, two-tailed paired t test). *Statistical significance ( P

    Journal: Diabetes

    Article Title: BMP-7 Induces Adult Human Pancreatic Exocrine-to-Endocrine Conversion

    doi: 10.2337/db15-0688

    Figure Lengend Snippet: Immunofluorescence and functional in vitro analysis of BMP-7–treated hNEPT. A , top row: BMP-7–induced cell aggregates coexpress insulin (green) and C-PEP (C-peptide, red). Channel merge and DAPI nuclear staining (blue) are shown at the right. Bottom row: Nuclear PDX-1 (red)/cytoplasmic insulin (green). Channel merge and nuclear staining (blue) are shown at the right. Scale bar, 50 μm. B , top row, from left to right: INS (insulin, green)/GLU (glucagon, red); INS (insulin, green)/PPY (red); INS (insulin, green)/SST (red). Bottom row, from left to right: INS (insulin, green)/NKX6.1 (red); INS (green)/MAFA (red); and INS (green)/PDX-1 (red)/DAPI (blue) in a representative insulin − /PDX-1 + cluster. Scale bars, 50 μm for top row and pictures 1 and 2 on the bottom row. Picture 3, bottom row: 100 μm. C : GSIS of control and treated (BMP-7) hNEPT ( n = 5 preparations). x -axis: L1, low glucose 1 (2.5 mmol/L); H, high glucose (20 mmol/L); L2, low glucose 2 (2.5 mmol/L). y -axis: human C-peptide (ng/μg of DNA). A second exposure to low glucose was performed to rule out false-positive results caused by “dumping” (the nonphysiological release of insulin). Data are presented as the mean ± SD ( n = 5). n.s., no significance ( P > 0.05, two-tailed paired t test). *Statistical significance ( P

    Article Snippet: These experiments suggest that BMP-7 acts through ALK3 in this setting.

    Techniques: Immunofluorescence, Functional Assay, In Vitro, Staining, Two Tailed Test

    BMP-7 acts partially through the SMAD pathway. A : C-peptide (ng/μg DNA) following hNEPT treatment with BMP-4. * P

    Journal: Diabetes

    Article Title: BMP-7 Induces Adult Human Pancreatic Exocrine-to-Endocrine Conversion

    doi: 10.2337/db15-0688

    Figure Lengend Snippet: BMP-7 acts partially through the SMAD pathway. A : C-peptide (ng/μg DNA) following hNEPT treatment with BMP-4. * P

    Article Snippet: These experiments suggest that BMP-7 acts through ALK3 in this setting.

    Techniques:

    BMP-7 induces endocrine-like colonies in hNEPT cultures. A : The administration of BMP-7 to hNEPT results in the formation of cellular clusters, in contrast to the mostly monolayer pattern that is observed in untreated controls (inset) at the same time point (10–12 days from the beginning of culture). Scale bars, 100 μm. B : An Incucyte Zoom instrument was used to capture still images of the same colony at four time points throughout the 10 days after BMP-7 addition. Scale bar, 50 μm. Representative markers of adult pancreatic cells ( C , left), pancreatic development ( C , right), and EMT ( D ) were analyzed by TaqMan Low Density Array qRT-PCR in BMP-7–treated and untreated hNEPT at the same time point after completion of the protocol. Values represent fold change (RQ ratios) vs. the untreated control. Following the Shapiro-Wilk normality test, statistical differences between RQ ratios were calculated by a two-tailed paired t test for normal distributions or a Wilcoxon signed rank test for nonparametric distributions. *** P

    Journal: Diabetes

    Article Title: BMP-7 Induces Adult Human Pancreatic Exocrine-to-Endocrine Conversion

    doi: 10.2337/db15-0688

    Figure Lengend Snippet: BMP-7 induces endocrine-like colonies in hNEPT cultures. A : The administration of BMP-7 to hNEPT results in the formation of cellular clusters, in contrast to the mostly monolayer pattern that is observed in untreated controls (inset) at the same time point (10–12 days from the beginning of culture). Scale bars, 100 μm. B : An Incucyte Zoom instrument was used to capture still images of the same colony at four time points throughout the 10 days after BMP-7 addition. Scale bar, 50 μm. Representative markers of adult pancreatic cells ( C , left), pancreatic development ( C , right), and EMT ( D ) were analyzed by TaqMan Low Density Array qRT-PCR in BMP-7–treated and untreated hNEPT at the same time point after completion of the protocol. Values represent fold change (RQ ratios) vs. the untreated control. Following the Shapiro-Wilk normality test, statistical differences between RQ ratios were calculated by a two-tailed paired t test for normal distributions or a Wilcoxon signed rank test for nonparametric distributions. *** P

    Article Snippet: These experiments suggest that BMP-7 acts through ALK3 in this setting.

    Techniques: TLDA Assay, Quantitative RT-PCR, Two Tailed Test

    Functional in vivo characterization of BMP-7–treated hNEPT. A : Human C-peptide determinations in nu/nu, STZ-treated mice transplanted with BMP-7–treated hNEPT, untreated hNEPT, or saline (sham) following the intraperitoneal glucose tolerance test. Left column: hNEPT/mouse recipient identifiers. Second/fifth columns: POD of serum human C-peptide (hC-pep determination). Third/fourth columns: human C-peptide concentrations (pmol/L) obtained prior to (0 min) and 60 min after glucose bolus injection (2.0 g/kg body wt) at PODs 25–39. Sixth/seventh columns: human C-peptide values obtained at PODs 108–122. Average glucose-stimulated human C-peptide release (0 and 60 min, represented by light gray and black columns, respectively) at PODs 25–39 ( B ) and PODs 108–122 ( C ). x -axis: C (saline), sham controls; C (untreated), control mice transplanted with untreated hNEPT; BMP-7, mice transplanted with BMP-7–treated NEPT. y -axis: C-peptide (pmol/L). Data are presented as the mean ± SD ( n = 12). * P

    Journal: Diabetes

    Article Title: BMP-7 Induces Adult Human Pancreatic Exocrine-to-Endocrine Conversion

    doi: 10.2337/db15-0688

    Figure Lengend Snippet: Functional in vivo characterization of BMP-7–treated hNEPT. A : Human C-peptide determinations in nu/nu, STZ-treated mice transplanted with BMP-7–treated hNEPT, untreated hNEPT, or saline (sham) following the intraperitoneal glucose tolerance test. Left column: hNEPT/mouse recipient identifiers. Second/fifth columns: POD of serum human C-peptide (hC-pep determination). Third/fourth columns: human C-peptide concentrations (pmol/L) obtained prior to (0 min) and 60 min after glucose bolus injection (2.0 g/kg body wt) at PODs 25–39. Sixth/seventh columns: human C-peptide values obtained at PODs 108–122. Average glucose-stimulated human C-peptide release (0 and 60 min, represented by light gray and black columns, respectively) at PODs 25–39 ( B ) and PODs 108–122 ( C ). x -axis: C (saline), sham controls; C (untreated), control mice transplanted with untreated hNEPT; BMP-7, mice transplanted with BMP-7–treated NEPT. y -axis: C-peptide (pmol/L). Data are presented as the mean ± SD ( n = 12). * P

    Article Snippet: These experiments suggest that BMP-7 acts through ALK3 in this setting.

    Techniques: Functional Assay, In Vivo, Mouse Assay, Injection

    Lineage-tracing studies. A : Tissue-specific promoters (PDX-1: β-cells and putative progenitors; β-cells; CAII, ductal; ELAS: elastase 3a, acinar) drive Cre expression. The reporter expresses dsRed (red) or EGFP (green) upon Cre-mediated loxP excision. Panels corresponding to each experiment are in parentheses. C-PEP, C-peptide (white); EGFP (green); dsRed (red); and channel merge (DAPI, blue) are shown for all experiments. B : PDX-1–Cre plus reporter. Abundant C-peptide + cells expressed EGFP (white arrows), suggesting a significant participation of PDX-1 + cells in BMP-7–induced C-peptide + cells. Another representative field is shown in higher magnification in C . D : RIP-Cre plus reporter. A smaller percentage of RIP-expressing cells contributed to the C-peptide + population. One such EGFP + /C-peptide + cell (white arrow) is shown among several other dsRed + /EGFP − /C-peptide + cells. E : RIP-Cre plus reporter. VIM, vimentin (white). Most green cells (white arrows) expressed vimentin, suggesting that residual β-cells typically undergo EMT. F : CAII-Cre plus reporter. Approximately 5% of C-peptide–expressing cells were EGFP tagged (white arrows). G : ELAS-Cre plus reporter. Several dsRed + /EGFP − /C-peptide + cells and two EGFP + /C-peptide + cells (white arrows) are shown. ImageJ-aided quantification of double positives indicated only a marginal acinar contribution to C-peptide + clusters. H : Table showing the relative estimated contribution (%) of each population. Scale bars for all panels, 50 μm.

    Journal: Diabetes

    Article Title: BMP-7 Induces Adult Human Pancreatic Exocrine-to-Endocrine Conversion

    doi: 10.2337/db15-0688

    Figure Lengend Snippet: Lineage-tracing studies. A : Tissue-specific promoters (PDX-1: β-cells and putative progenitors; β-cells; CAII, ductal; ELAS: elastase 3a, acinar) drive Cre expression. The reporter expresses dsRed (red) or EGFP (green) upon Cre-mediated loxP excision. Panels corresponding to each experiment are in parentheses. C-PEP, C-peptide (white); EGFP (green); dsRed (red); and channel merge (DAPI, blue) are shown for all experiments. B : PDX-1–Cre plus reporter. Abundant C-peptide + cells expressed EGFP (white arrows), suggesting a significant participation of PDX-1 + cells in BMP-7–induced C-peptide + cells. Another representative field is shown in higher magnification in C . D : RIP-Cre plus reporter. A smaller percentage of RIP-expressing cells contributed to the C-peptide + population. One such EGFP + /C-peptide + cell (white arrow) is shown among several other dsRed + /EGFP − /C-peptide + cells. E : RIP-Cre plus reporter. VIM, vimentin (white). Most green cells (white arrows) expressed vimentin, suggesting that residual β-cells typically undergo EMT. F : CAII-Cre plus reporter. Approximately 5% of C-peptide–expressing cells were EGFP tagged (white arrows). G : ELAS-Cre plus reporter. Several dsRed + /EGFP − /C-peptide + cells and two EGFP + /C-peptide + cells (white arrows) are shown. ImageJ-aided quantification of double positives indicated only a marginal acinar contribution to C-peptide + clusters. H : Table showing the relative estimated contribution (%) of each population. Scale bars for all panels, 50 μm.

    Article Snippet: These experiments suggest that BMP-7 acts through ALK3 in this setting.

    Techniques: Expressing

    Renal immunostaining for TGF- β 1 (a)–(c), fibronectin (d)–(f), collagen IV (g)–(i), and BMP-7 (j)–(l) expression in normal or STZ-diabetic mice receiving 4 weeks of XCHT treatment ( n = 6 per group). Original magnification, 200x. Representative micrographs are shown.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

    doi: 10.1155/2012/984024

    Figure Lengend Snippet: Renal immunostaining for TGF- β 1 (a)–(c), fibronectin (d)–(f), collagen IV (g)–(i), and BMP-7 (j)–(l) expression in normal or STZ-diabetic mice receiving 4 weeks of XCHT treatment ( n = 6 per group). Original magnification, 200x. Representative micrographs are shown.

    Article Snippet: Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) RT-PCR was performed to determine the mRNA expression levels of TGF-β 1, fibronectin, collagen IV, and BMP-7.

    Techniques: Immunostaining, Expressing, Mouse Assay

    Effect of XCHT on TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in the kidney of normal or STZ-diabetic mice after 4 weeks of treatment ( n = 6 in each group). (a) The mRNA expression of TGF- β 1, fibronectin, collagen IV, and BMP-7 in the kidney of mice was detected using RT-PCR. β -actin mRNA expression was included as internal control. (b) Western blot analysis of TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in the kidney of the test mice. Representative data are shown.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

    doi: 10.1155/2012/984024

    Figure Lengend Snippet: Effect of XCHT on TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in the kidney of normal or STZ-diabetic mice after 4 weeks of treatment ( n = 6 in each group). (a) The mRNA expression of TGF- β 1, fibronectin, collagen IV, and BMP-7 in the kidney of mice was detected using RT-PCR. β -actin mRNA expression was included as internal control. (b) Western blot analysis of TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in the kidney of the test mice. Representative data are shown.

    Article Snippet: Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) RT-PCR was performed to determine the mRNA expression levels of TGF-β 1, fibronectin, collagen IV, and BMP-7.

    Techniques: Expressing, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Effect of XCHT on TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in RMCs. RMCs were cultured in normal-glucose (NG; 5 mmol/L) or high-glucose (HG; 30 mmol/L) medium in the presence of 50 μ g/mL of XCHT for 24 h. (a) The mRNA expression of TGF- β 1, fibronectin, collagen IV, and BMP-7 in RMCs detected by RT-PCR. β -Actin mRNA expression is used as an internal control. (b) Western blot analysis of TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in RMCs.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Renal Protective Effect of Xiao-Chai-Hu-Tang on Diabetic Nephropathy of Type 1-Diabetic Mice

    doi: 10.1155/2012/984024

    Figure Lengend Snippet: Effect of XCHT on TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in RMCs. RMCs were cultured in normal-glucose (NG; 5 mmol/L) or high-glucose (HG; 30 mmol/L) medium in the presence of 50 μ g/mL of XCHT for 24 h. (a) The mRNA expression of TGF- β 1, fibronectin, collagen IV, and BMP-7 in RMCs detected by RT-PCR. β -Actin mRNA expression is used as an internal control. (b) Western blot analysis of TGF- β 1, fibronectin, collagen IV, and BMP-7 expression in RMCs.

    Article Snippet: Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) RT-PCR was performed to determine the mRNA expression levels of TGF-β 1, fibronectin, collagen IV, and BMP-7.

    Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Immunohistochemical staining of differentially expressed gene products. Normal ovaries ( A , D , G , J , M ), serous papillary ovarian carcinoma ( B , E , H , K , N ), and serous papillary ovarian carcinoma metastatic to the omentum ( C , F , I , L , O ) tissues were stained with a mAb against the β1 integrin subunit ( A–C ), normal mouse IgG ( D–F ), and antibodies against: the β8 integrin subunit ( G–I ), BMP-7 ( J–L ), and claudin-4 ( M–O ). Original magnifications, ×60.

    Journal: The American Journal of Pathology

    Article Title: Differential Gene Expression in Ovarian Carcinoma

    doi:

    Figure Lengend Snippet: Immunohistochemical staining of differentially expressed gene products. Normal ovaries ( A , D , G , J , M ), serous papillary ovarian carcinoma ( B , E , H , K , N ), and serous papillary ovarian carcinoma metastatic to the omentum ( C , F , I , L , O ) tissues were stained with a mAb against the β1 integrin subunit ( A–C ), normal mouse IgG ( D–F ), and antibodies against: the β8 integrin subunit ( G–I ), BMP-7 ( J–L ), and claudin-4 ( M–O ). Original magnifications, ×60.

    Article Snippet: Based on these selection criteria, seven genes were chosen for further analysis: the β8 integrin subunit, BMP-7, CRABP-1, claudin-4, COL IX α2, FOX J1, and S100 calcium-binding protein A1 (S100A1).

    Techniques: Immunohistochemistry, Staining

    Immunohistochemistry for bone morphogenic protein (BMP; [a–c and g–l] 100× magnification; [d and f] 400× magnification). (a, d, g, and j) BMP-2, (b, e, h, and k) BMP-4, and (c, f, i, and l) BMP-7. IPB cells were strongly positively stained for BMP-4 and produced a negative result after staining for BMP-2 and BMP-7. IPB, inverted papilloma with new bone formation; IPC, inverted papilloma without bone formation (common type); NP, nasal polyp. *Bone tissue.

    Journal: Allergy & Rhinology

    Article Title: Expression of bone morphogenic protein in sinonasal inverted papilloma with new bone formation

    doi: 10.2500/ar.2011.2.0004

    Figure Lengend Snippet: Immunohistochemistry for bone morphogenic protein (BMP; [a–c and g–l] 100× magnification; [d and f] 400× magnification). (a, d, g, and j) BMP-2, (b, e, h, and k) BMP-4, and (c, f, i, and l) BMP-7. IPB cells were strongly positively stained for BMP-4 and produced a negative result after staining for BMP-2 and BMP-7. IPB, inverted papilloma with new bone formation; IPC, inverted papilloma without bone formation (common type); NP, nasal polyp. *Bone tissue.

    Article Snippet: BMP-7 plays a crucial role in osteoblast differentiation, eye development, renal development/repair, and craniofacial development and may play a role in cerebral protection from ischemic stroke.

    Techniques: Immunohistochemistry, Staining, Produced