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  • 99
    Lonza bmmscs
    Bmmscs, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC bmmscs murine raw264 7 macrophages
    Bmmscs Murine Raw264 7 Macrophages, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    R&D Systems bmp 2
    Representative radiograph and in vivo micro-CT reconstructions of diaphyseal defects for the <t>−BMMSC/−BMP-2</t> (A) , −BMMSC/+BMP-2 (B) , and +BMMSC/+BMP-2 groups (C) at 4, 8, and 12 weeks. (D) Bridging rates as determined by three independent
    Bmp 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 2993 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ScienCell mouse bmmscs
    The effect of <t>circRNA_014511</t> on apoptosis of <t>BMMSCs.</t> (A) Western blot assessment of the expressions of apoptosis-related proteins p53, Bcl-2, and Mcl-1. (B) Flow cytometry detection of apoptosis in cells of each group.
    Mouse Bmmscs, supplied by ScienCell, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bmmsc medium
    The effect of <t>circRNA_014511</t> on apoptosis of <t>BMMSCs.</t> (A) Western blot assessment of the expressions of apoptosis-related proteins p53, Bcl-2, and Mcl-1. (B) Flow cytometry detection of apoptosis in cells of each group.
    Bmmsc Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Exosome Diagnostics bmmscs carrying mir 124 3p
    Schematic image of potential molecular mechanisms. Exosomes carrying <t>miR-124-3p</t> derived from <t>BMMSCs</t> enhance M2 macrophage polarization by regulating Ern1, thus alleviating spinal injury in SCIRI. In SCIRI rats, exosomes were derived from BMMSCs expressing miR-124-3p, which elevated Arg1, Ym1, and Fizz expression and promoted M2 macrophage polarization by inhibiting Ern1 expression. Furthermore, exosomal miR-124-3p suppressed cell apoptosis and nerve injury induced by SCIRI
    Bmmscs Carrying Mir 124 3p, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bmmscs  (ATCC)
    92
    ATCC bmmscs
    hESC‐FN‐MSCs are capable of in vivo differentiating into cells of multiple lineages. a) Macroscopic appearance of adipose tissue newly formed in nude mice. Human extracellular matrix powders containing <t>ASCs,</t> <t>BMMSCs,</t> and hESC‐FN‐MSCs were implanted into the back of each nude mouse for 5 weeks. The formed tissue in the hESC‐FN‐MSC‐injected group was larger than those in the ASC‐ and BMMSC‐injected groups ( n = 4–5). b) The weight of adipose tissue formed in the group injected with hESC‐FN‐MSCs was compared with that formed in the groups injected with ASCs and BMMSCs. The formed tissue in the hESC‐FN‐MSC‐injected group was significantly heavier than that in the control (ECM‐only), but was not significantly different compared to the tissue in both the ASC‐ and BMMSC‐injected groups. n = 4, mean ± s.d., ns, not significant, and * P
    Bmmscs, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    AllCells LLC human bmmsc
    hESC‐FN‐MSCs are capable of in vivo differentiating into cells of multiple lineages. a) Macroscopic appearance of adipose tissue newly formed in nude mice. Human extracellular matrix powders containing <t>ASCs,</t> <t>BMMSCs,</t> and hESC‐FN‐MSCs were implanted into the back of each nude mouse for 5 weeks. The formed tissue in the hESC‐FN‐MSC‐injected group was larger than those in the ASC‐ and BMMSC‐injected groups ( n = 4–5). b) The weight of adipose tissue formed in the group injected with hESC‐FN‐MSCs was compared with that formed in the groups injected with ASCs and BMMSCs. The formed tissue in the hESC‐FN‐MSC‐injected group was significantly heavier than that in the control (ECM‐only), but was not significantly different compared to the tissue in both the ASC‐ and BMMSC‐injected groups. n = 4, mean ± s.d., ns, not significant, and * P
    Human Bmmsc, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ScienCell medium human bmmsc
    hESC‐FN‐MSCs are capable of in vivo differentiating into cells of multiple lineages. a) Macroscopic appearance of adipose tissue newly formed in nude mice. Human extracellular matrix powders containing <t>ASCs,</t> <t>BMMSCs,</t> and hESC‐FN‐MSCs were implanted into the back of each nude mouse for 5 weeks. The formed tissue in the hESC‐FN‐MSC‐injected group was larger than those in the ASC‐ and BMMSC‐injected groups ( n = 4–5). b) The weight of adipose tissue formed in the group injected with hESC‐FN‐MSCs was compared with that formed in the groups injected with ASCs and BMMSCs. The formed tissue in the hESC‐FN‐MSC‐injected group was significantly heavier than that in the control (ECM‐only), but was not significantly different compared to the tissue in both the ASC‐ and BMMSC‐injected groups. n = 4, mean ± s.d., ns, not significant, and * P
    Medium Human Bmmsc, supplied by ScienCell, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Exosome Diagnostics bmmsc derived exosomes electron microscopy exosome pellets
    Real time PCR analysis. Expression levels of mRNA depicted for (A) MZB1 (B) CXCL8, were measured in activated B cells with or without <t>exosomes.</t> The graphs show the results of 6 exosome batches (MZB1 exosome batches 6, 8, 10, 12, 9, 3, respectively; CXCL8 exosome batches 6, 9, 10, 8, 12, 3, respectively). GAPDH was used as internal controls for targeting mRNA expression, and data are expressed as the mean of triplicate samples ± S.E.
    Bmmsc Derived Exosomes Electron Microscopy Exosome Pellets, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Exosome Diagnostics bmmsc derived exosome agent
    Real time PCR analysis. Expression levels of mRNA depicted for (A) MZB1 (B) CXCL8, were measured in activated B cells with or without <t>exosomes.</t> The graphs show the results of 6 exosome batches (MZB1 exosome batches 6, 8, 10, 12, 9, 3, respectively; CXCL8 exosome batches 6, 9, 10, 8, 12, 3, respectively). GAPDH was used as internal controls for targeting mRNA expression, and data are expressed as the mean of triplicate samples ± S.E.
    Bmmsc Derived Exosome Agent, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher bmmsc material
    Real time PCR analysis. Expression levels of mRNA depicted for (A) MZB1 (B) CXCL8, were measured in activated B cells with or without <t>exosomes.</t> The graphs show the results of 6 exosome batches (MZB1 exosome batches 6, 8, 10, 12, 9, 3, respectively; CXCL8 exosome batches 6, 9, 10, 8, 12, 3, respectively). GAPDH was used as internal controls for targeting mRNA expression, and data are expressed as the mean of triplicate samples ± S.E.
    Bmmsc Material, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher bmmsc qualified fetal bovine serum
    Real time PCR analysis. Expression levels of mRNA depicted for (A) MZB1 (B) CXCL8, were measured in activated B cells with or without <t>exosomes.</t> The graphs show the results of 6 exosome batches (MZB1 exosome batches 6, 8, 10, 12, 9, 3, respectively; CXCL8 exosome batches 6, 9, 10, 8, 12, 3, respectively). GAPDH was used as internal controls for targeting mRNA expression, and data are expressed as the mean of triplicate samples ± S.E.
    Bmmsc Qualified Fetal Bovine Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ScienCell bmmsc zq0465 cells
    Real time PCR analysis. Expression levels of mRNA depicted for (A) MZB1 (B) CXCL8, were measured in activated B cells with or without <t>exosomes.</t> The graphs show the results of 6 exosome batches (MZB1 exosome batches 6, 8, 10, 12, 9, 3, respectively; CXCL8 exosome batches 6, 9, 10, 8, 12, 3, respectively). GAPDH was used as internal controls for targeting mRNA expression, and data are expressed as the mean of triplicate samples ± S.E.
    Bmmsc Zq0465 Cells, supplied by ScienCell, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Cytoskeleton Inc bmmscs cytoskeleton
    <t>Cytoskeleton</t> (F-actin fibers) organization in <t>bmMSCs</t> after exposure to 0, 5, 10, and 20 μ g/mL ox-LDL for 6 hours.
    Bmmscs Cytoskeleton, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cambrex bmmscs
    <t>Cytoskeleton</t> (F-actin fibers) organization in <t>bmMSCs</t> after exposure to 0, 5, 10, and 20 μ g/mL ox-LDL for 6 hours.
    Bmmscs, supplied by Cambrex, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Essen Bioscience bmmscs
    <t>Cytoskeleton</t> (F-actin fibers) organization in <t>bmMSCs</t> after exposure to 0, 5, 10, and 20 μ g/mL ox-LDL for 6 hours.
    Bmmscs, supplied by Essen Bioscience, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Exosome Diagnostics bmmscs
    Characteristic of exosomes isolated from mouse <t>BMMSCs.</t> a Microvesicles in MSCs were analyzed by TEM. b Immunofluorescent staining of CD63 and CD9 in MSCs. c Differential centrifugation procedure for the isolation of exosomes from culture supernatants of MSCs (SN). d Isolated exosomes from control and IFN-γ-primed MSCs were analyzed by TEM. e Exosomes numbers isolated from control and IFN-γ-primed MSCs was analyzed by EXOCEP <t>exosome</t> quantitation kit. f The expression of CD81 and CD9 in exosomes from control and IFN-γ-primed MSCs were analyzed by western blotting. Scale bar a : 500 nm, b : 20 μm, and d : 100 nm. * P
    Bmmscs, supplied by Exosome Diagnostics, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PromoCell bmmscs
    Characteristic of exosomes isolated from mouse <t>BMMSCs.</t> a Microvesicles in MSCs were analyzed by TEM. b Immunofluorescent staining of CD63 and CD9 in MSCs. c Differential centrifugation procedure for the isolation of exosomes from culture supernatants of MSCs (SN). d Isolated exosomes from control and IFN-γ-primed MSCs were analyzed by TEM. e Exosomes numbers isolated from control and IFN-γ-primed MSCs was analyzed by EXOCEP <t>exosome</t> quantitation kit. f The expression of CD81 and CD9 in exosomes from control and IFN-γ-primed MSCs were analyzed by western blotting. Scale bar a : 500 nm, b : 20 μm, and d : 100 nm. * P
    Bmmscs, supplied by PromoCell, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Siemens AG bmmscs
    Characteristic of exosomes isolated from mouse <t>BMMSCs.</t> a Microvesicles in MSCs were analyzed by TEM. b Immunofluorescent staining of CD63 and CD9 in MSCs. c Differential centrifugation procedure for the isolation of exosomes from culture supernatants of MSCs (SN). d Isolated exosomes from control and IFN-γ-primed MSCs were analyzed by TEM. e Exosomes numbers isolated from control and IFN-γ-primed MSCs was analyzed by EXOCEP <t>exosome</t> quantitation kit. f The expression of CD81 and CD9 in exosomes from control and IFN-γ-primed MSCs were analyzed by western blotting. Scale bar a : 500 nm, b : 20 μm, and d : 100 nm. * P
    Bmmscs, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher bmmscs
    Characteristic of exosomes isolated from mouse <t>BMMSCs.</t> a Microvesicles in MSCs were analyzed by TEM. b Immunofluorescent staining of CD63 and CD9 in MSCs. c Differential centrifugation procedure for the isolation of exosomes from culture supernatants of MSCs (SN). d Isolated exosomes from control and IFN-γ-primed MSCs were analyzed by TEM. e Exosomes numbers isolated from control and IFN-γ-primed MSCs was analyzed by EXOCEP <t>exosome</t> quantitation kit. f The expression of CD81 and CD9 in exosomes from control and IFN-γ-primed MSCs were analyzed by western blotting. Scale bar a : 500 nm, b : 20 μm, and d : 100 nm. * P
    Bmmscs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Vital River Laboratories bmmscs
    MiR-200b in HBM-exo targets Hmgb3 to attenuate the inflammatory injury of IECs. a TargetScan, miRWalk, and miRDB database predicted the microRNAs targeting the 3′ UTR of Hmgb3 . b QRT-PCR was used to detect the relative expression levels of miR-200b, miR-17, miR-139, miR-429, miR-191a, and miR-214a in TNF-α-exo, BM-exo, and HBM-exo groups (normalized by U6, fold change to TNF-α-exo, n = 5). c QRT-PCR was used to detect the relative expression of miR-200b in the Mock, TNF-α, TNF-α-exo, BM-exo, and HBM-exo groups (fold change relative to the Mock group, n = 4). d , e The plasmids HMGB3-WT (wild-type), HMGB3-MUT (mutant-type), positive control (Positive Con.) and negative control (Negative Con.) were constructed. Dual luciferase reporter assays were used to verify the targeted binding relationship between miR-200b and the 3′ UTR region of Hmgb3 in HEK293T cells and IEC-6s ( n = 3). f , g The protective effect of <t>overexpression</t> of miR-200b on inflammatory injury of IEC-6s. Transfection of miR-200b mimic and Negative Con., western blotting to verify the Mock group, Control group under TNF-α damage, and the Negative Con group. The relative expression levels of HMGB3, phosphorylated (p-) JNK, and other proteins in IEC-6s of the control and miR-200b mimic groups ( n = 3); h , i Interference with miR-200b expression to block the protective effect of HBM-exo on inflammatory IEC-6s. Transfection of an miR-200b inhibitor and Negative Con. After treatment, the relative expression levels of HMGB3 and p-JNK were detected in the model of IEC-6s protected by HBM-exo ( n = 3). j The relative expression levels of miR-200b in <t>BMMSCs,</t> HO-1/BMMSCs, TNF-α + BMMSCs, TNF-α + HO-1/BMMSCs cells, and exocrine bodies were detected (fold change to BMMSCs, n = 5). * P
    Bmmscs, supplied by Vital River Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Hitachi Ltd human bmmsc clusters
    MiR-200b in HBM-exo targets Hmgb3 to attenuate the inflammatory injury of IECs. a TargetScan, miRWalk, and miRDB database predicted the microRNAs targeting the 3′ UTR of Hmgb3 . b QRT-PCR was used to detect the relative expression levels of miR-200b, miR-17, miR-139, miR-429, miR-191a, and miR-214a in TNF-α-exo, BM-exo, and HBM-exo groups (normalized by U6, fold change to TNF-α-exo, n = 5). c QRT-PCR was used to detect the relative expression of miR-200b in the Mock, TNF-α, TNF-α-exo, BM-exo, and HBM-exo groups (fold change relative to the Mock group, n = 4). d , e The plasmids HMGB3-WT (wild-type), HMGB3-MUT (mutant-type), positive control (Positive Con.) and negative control (Negative Con.) were constructed. Dual luciferase reporter assays were used to verify the targeted binding relationship between miR-200b and the 3′ UTR region of Hmgb3 in HEK293T cells and IEC-6s ( n = 3). f , g The protective effect of <t>overexpression</t> of miR-200b on inflammatory injury of IEC-6s. Transfection of miR-200b mimic and Negative Con., western blotting to verify the Mock group, Control group under TNF-α damage, and the Negative Con group. The relative expression levels of HMGB3, phosphorylated (p-) JNK, and other proteins in IEC-6s of the control and miR-200b mimic groups ( n = 3); h , i Interference with miR-200b expression to block the protective effect of HBM-exo on inflammatory IEC-6s. Transfection of an miR-200b inhibitor and Negative Con. After treatment, the relative expression levels of HMGB3 and p-JNK were detected in the model of IEC-6s protected by HBM-exo ( n = 3). j The relative expression levels of miR-200b in <t>BMMSCs,</t> HO-1/BMMSCs, TNF-α + BMMSCs, TNF-α + HO-1/BMMSCs cells, and exocrine bodies were detected (fold change to BMMSCs, n = 5). * P
    Human Bmmsc Clusters, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher human bmmsc
    MiR-200b in HBM-exo targets Hmgb3 to attenuate the inflammatory injury of IECs. a TargetScan, miRWalk, and miRDB database predicted the microRNAs targeting the 3′ UTR of Hmgb3 . b QRT-PCR was used to detect the relative expression levels of miR-200b, miR-17, miR-139, miR-429, miR-191a, and miR-214a in TNF-α-exo, BM-exo, and HBM-exo groups (normalized by U6, fold change to TNF-α-exo, n = 5). c QRT-PCR was used to detect the relative expression of miR-200b in the Mock, TNF-α, TNF-α-exo, BM-exo, and HBM-exo groups (fold change relative to the Mock group, n = 4). d , e The plasmids HMGB3-WT (wild-type), HMGB3-MUT (mutant-type), positive control (Positive Con.) and negative control (Negative Con.) were constructed. Dual luciferase reporter assays were used to verify the targeted binding relationship between miR-200b and the 3′ UTR region of Hmgb3 in HEK293T cells and IEC-6s ( n = 3). f , g The protective effect of <t>overexpression</t> of miR-200b on inflammatory injury of IEC-6s. Transfection of miR-200b mimic and Negative Con., western blotting to verify the Mock group, Control group under TNF-α damage, and the Negative Con group. The relative expression levels of HMGB3, phosphorylated (p-) JNK, and other proteins in IEC-6s of the control and miR-200b mimic groups ( n = 3); h , i Interference with miR-200b expression to block the protective effect of HBM-exo on inflammatory IEC-6s. Transfection of an miR-200b inhibitor and Negative Con. After treatment, the relative expression levels of HMGB3 and p-JNK were detected in the model of IEC-6s protected by HBM-exo ( n = 3). j The relative expression levels of miR-200b in <t>BMMSCs,</t> HO-1/BMMSCs, TNF-α + BMMSCs, TNF-α + HO-1/BMMSCs cells, and exocrine bodies were detected (fold change to BMMSCs, n = 5). * P
    Human Bmmsc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative radiograph and in vivo micro-CT reconstructions of diaphyseal defects for the −BMMSC/−BMP-2 (A) , −BMMSC/+BMP-2 (B) , and +BMMSC/+BMP-2 groups (C) at 4, 8, and 12 weeks. (D) Bridging rates as determined by three independent

    Journal: Tissue Engineering. Part A

    Article Title: Effect of Cell Origin and Timing of Delivery for Stem Cell-Based Bone Tissue Engineering Using Biologically Functionalized Hydrogels

    doi: 10.1089/ten.tea.2014.0057

    Figure Lengend Snippet: Representative radiograph and in vivo micro-CT reconstructions of diaphyseal defects for the −BMMSC/−BMP-2 (A) , −BMMSC/+BMP-2 (B) , and +BMMSC/+BMP-2 groups (C) at 4, 8, and 12 weeks. (D) Bridging rates as determined by three independent

    Article Snippet: Incorporation of BMMSCs (+BMMSC/+BMP-2) resulted in significantly higher bone volume at 8 weeks for immediate delivery samples; this group was significantly greater than the −BMMSC/+BMP-2 and −BMMSC/−BMP-2 hydrogels, as well as the delayed +BMMSC/+BMP-2 implantation group.

    Techniques: In Vivo, Micro-CT

    The effect of circRNA_014511 on apoptosis of BMMSCs. (A) Western blot assessment of the expressions of apoptosis-related proteins p53, Bcl-2, and Mcl-1. (B) Flow cytometry detection of apoptosis in cells of each group.

    Journal: Bosnian Journal of Basic Medical Sciences

    Article Title: CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p

    doi: 10.17305/bjbms.2019.3935

    Figure Lengend Snippet: The effect of circRNA_014511 on apoptosis of BMMSCs. (A) Western blot assessment of the expressions of apoptosis-related proteins p53, Bcl-2, and Mcl-1. (B) Flow cytometry detection of apoptosis in cells of each group.

    Article Snippet: To sum up, through in vitro experiments in mouse BMMSCs, this study demonstrated that circRNA_014511 could inhibit the expression of P53 through the endogenous competitive combination of mmu-miR-29b-2-5p, and induced the changes in multiple apoptosis-related proteins and cell-cycle-related proteins, which decreased the radiosensitivity of the cells.

    Techniques: Western Blot, Flow Cytometry, Cytometry

    The regulation of p53 expression by the combination of circRNA_014511 and mmu-miR-29b-2-5p in BMMSCs. (A) and (B), quantitative fluorescence PCR detection of the expressions of circRNA_014511 and mmu-miR-29b-2-5p in the cells of each group. (C) and (D) results of dual luciferase reporter assay. (E) and (F) Western blot assessment of p53 expression.

    Journal: Bosnian Journal of Basic Medical Sciences

    Article Title: CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p

    doi: 10.17305/bjbms.2019.3935

    Figure Lengend Snippet: The regulation of p53 expression by the combination of circRNA_014511 and mmu-miR-29b-2-5p in BMMSCs. (A) and (B), quantitative fluorescence PCR detection of the expressions of circRNA_014511 and mmu-miR-29b-2-5p in the cells of each group. (C) and (D) results of dual luciferase reporter assay. (E) and (F) Western blot assessment of p53 expression.

    Article Snippet: To sum up, through in vitro experiments in mouse BMMSCs, this study demonstrated that circRNA_014511 could inhibit the expression of P53 through the endogenous competitive combination of mmu-miR-29b-2-5p, and induced the changes in multiple apoptosis-related proteins and cell-cycle-related proteins, which decreased the radiosensitivity of the cells.

    Techniques: Expressing, Fluorescence, Polymerase Chain Reaction, Luciferase, Reporter Assay, Western Blot

    The effect of circRNA_014511 on cell cycle of BMMSCs. (A) Flow cytometry assessment of cell cycle in cells of each group.(B) Western blot assessment of the expressions of cell cycle-related proteins P21, GADD45A, and Cyclin B1.

    Journal: Bosnian Journal of Basic Medical Sciences

    Article Title: CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p

    doi: 10.17305/bjbms.2019.3935

    Figure Lengend Snippet: The effect of circRNA_014511 on cell cycle of BMMSCs. (A) Flow cytometry assessment of cell cycle in cells of each group.(B) Western blot assessment of the expressions of cell cycle-related proteins P21, GADD45A, and Cyclin B1.

    Article Snippet: To sum up, through in vitro experiments in mouse BMMSCs, this study demonstrated that circRNA_014511 could inhibit the expression of P53 through the endogenous competitive combination of mmu-miR-29b-2-5p, and induced the changes in multiple apoptosis-related proteins and cell-cycle-related proteins, which decreased the radiosensitivity of the cells.

    Techniques: Flow Cytometry, Cytometry, Western Blot

    Effect of circRNA_014511 on the radiosensitivity of BMMSCs.

    Journal: Bosnian Journal of Basic Medical Sciences

    Article Title: CircRNA_014511 affects the radiosensitivity of bone marrow mesenchymal stem cells by binding to miR-29b-2-5p

    doi: 10.17305/bjbms.2019.3935

    Figure Lengend Snippet: Effect of circRNA_014511 on the radiosensitivity of BMMSCs.

    Article Snippet: To sum up, through in vitro experiments in mouse BMMSCs, this study demonstrated that circRNA_014511 could inhibit the expression of P53 through the endogenous competitive combination of mmu-miR-29b-2-5p, and induced the changes in multiple apoptosis-related proteins and cell-cycle-related proteins, which decreased the radiosensitivity of the cells.

    Techniques:

    Schematic image of potential molecular mechanisms. Exosomes carrying miR-124-3p derived from BMMSCs enhance M2 macrophage polarization by regulating Ern1, thus alleviating spinal injury in SCIRI. In SCIRI rats, exosomes were derived from BMMSCs expressing miR-124-3p, which elevated Arg1, Ym1, and Fizz expression and promoted M2 macrophage polarization by inhibiting Ern1 expression. Furthermore, exosomal miR-124-3p suppressed cell apoptosis and nerve injury induced by SCIRI

    Journal: Arthritis Research & Therapy

    Article Title: Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization

    doi: 10.1186/s13075-020-2146-x

    Figure Lengend Snippet: Schematic image of potential molecular mechanisms. Exosomes carrying miR-124-3p derived from BMMSCs enhance M2 macrophage polarization by regulating Ern1, thus alleviating spinal injury in SCIRI. In SCIRI rats, exosomes were derived from BMMSCs expressing miR-124-3p, which elevated Arg1, Ym1, and Fizz expression and promoted M2 macrophage polarization by inhibiting Ern1 expression. Furthermore, exosomal miR-124-3p suppressed cell apoptosis and nerve injury induced by SCIRI

    Article Snippet: Exosome derived from BMMSCs carrying miR-124-3p modulates Ern1 and augments polarization of M2 macrophage in vivo With an aim to further verify the regulation of miR-124-3p on Ern1 and M2 macrophage polarization in SCIRI in vivo, SCIRI rats were injected with exosomes derived from BMMSCs carrying miR-124-3p.

    Techniques: Derivative Assay, Expressing

    Expression of Ern1 in macrophages was mediated by exosomal miR-124-3p derived from BMMSCs. a Macrophages ingesting exosomes observed by confocal fluorescence microscope (× 200). b Expression of miR-124-3p in BMMSCs and exosomes detected by RT-qPCR; * p

    Journal: Arthritis Research & Therapy

    Article Title: Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization

    doi: 10.1186/s13075-020-2146-x

    Figure Lengend Snippet: Expression of Ern1 in macrophages was mediated by exosomal miR-124-3p derived from BMMSCs. a Macrophages ingesting exosomes observed by confocal fluorescence microscope (× 200). b Expression of miR-124-3p in BMMSCs and exosomes detected by RT-qPCR; * p

    Article Snippet: Exosome derived from BMMSCs carrying miR-124-3p modulates Ern1 and augments polarization of M2 macrophage in vivo With an aim to further verify the regulation of miR-124-3p on Ern1 and M2 macrophage polarization in SCIRI in vivo, SCIRI rats were injected with exosomes derived from BMMSCs carrying miR-124-3p.

    Techniques: Expressing, Derivative Assay, Fluorescence, Microscopy, Quantitative RT-PCR

    SCIRI could be alleviated by miR-124-3p overexpression. a Evaluation of hindlimb motor function in rats. b Cell apoptosis in spinal cord tissues determined by TUNEL assay (× 400). c BSCB integrity tested by EB staining (× 400). d H E staining of spinal cord tissues of rats (× 200). * p

    Journal: Arthritis Research & Therapy

    Article Title: Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization

    doi: 10.1186/s13075-020-2146-x

    Figure Lengend Snippet: SCIRI could be alleviated by miR-124-3p overexpression. a Evaluation of hindlimb motor function in rats. b Cell apoptosis in spinal cord tissues determined by TUNEL assay (× 400). c BSCB integrity tested by EB staining (× 400). d H E staining of spinal cord tissues of rats (× 200). * p

    Article Snippet: Exosome derived from BMMSCs carrying miR-124-3p modulates Ern1 and augments polarization of M2 macrophage in vivo With an aim to further verify the regulation of miR-124-3p on Ern1 and M2 macrophage polarization in SCIRI in vivo, SCIRI rats were injected with exosomes derived from BMMSCs carrying miR-124-3p.

    Techniques: Over Expression, TUNEL Assay, Staining

    M2 macrophage polarization was induced by miR-124-3p via regulation of Ern1. a Markers of M2 macrophage polarization (Arg1, Ym1, and Fizz) expression determined by western blot analysis; a * p

    Journal: Arthritis Research & Therapy

    Article Title: Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization

    doi: 10.1186/s13075-020-2146-x

    Figure Lengend Snippet: M2 macrophage polarization was induced by miR-124-3p via regulation of Ern1. a Markers of M2 macrophage polarization (Arg1, Ym1, and Fizz) expression determined by western blot analysis; a * p

    Article Snippet: Exosome derived from BMMSCs carrying miR-124-3p modulates Ern1 and augments polarization of M2 macrophage in vivo With an aim to further verify the regulation of miR-124-3p on Ern1 and M2 macrophage polarization in SCIRI in vivo, SCIRI rats were injected with exosomes derived from BMMSCs carrying miR-124-3p.

    Techniques: Expressing, Western Blot

    In vivo experiment demonstrating exosomal miR-124-3p derived from BMMSCs regulated Ern1 and polarization of M2 macrophage in SCIRI rats. a Ern1, Arg1, Ym1, and Fizz expression in rats spinal cord tissues determined using RT-qPCR. b Ern1, Arg1, Ym1, and Fizz protein levels in rat spinal cord tissues measured by western blot analysis. c Immunohistochemistry assay of Ern1, Arg1, Ym1, and Fizz expression in rat spinal cord tissues (× 400). d Immunofluorescence assay of Ern1, Arg1, Ym1, and Fizz expression in rats spinal cord tissues (× 400). * p

    Journal: Arthritis Research & Therapy

    Article Title: Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization

    doi: 10.1186/s13075-020-2146-x

    Figure Lengend Snippet: In vivo experiment demonstrating exosomal miR-124-3p derived from BMMSCs regulated Ern1 and polarization of M2 macrophage in SCIRI rats. a Ern1, Arg1, Ym1, and Fizz expression in rats spinal cord tissues determined using RT-qPCR. b Ern1, Arg1, Ym1, and Fizz protein levels in rat spinal cord tissues measured by western blot analysis. c Immunohistochemistry assay of Ern1, Arg1, Ym1, and Fizz expression in rat spinal cord tissues (× 400). d Immunofluorescence assay of Ern1, Arg1, Ym1, and Fizz expression in rats spinal cord tissues (× 400). * p

    Article Snippet: Exosome derived from BMMSCs carrying miR-124-3p modulates Ern1 and augments polarization of M2 macrophage in vivo With an aim to further verify the regulation of miR-124-3p on Ern1 and M2 macrophage polarization in SCIRI in vivo, SCIRI rats were injected with exosomes derived from BMMSCs carrying miR-124-3p.

    Techniques: In Vivo, Derivative Assay, Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemistry, Immunofluorescence

    Ern1 was targeted by miR-124-3p. a Possible target genes of miR-124-3p predicted by bioinformatic analysis. b Expression of miR-124-3p in SCIRI tissues detected by RT-qPCR, * p

    Journal: Arthritis Research & Therapy

    Article Title: Bone marrow mesenchymal stem cell-derived exosomal microRNA-124-3p attenuates neurological damage in spinal cord ischemia-reperfusion injury by downregulating Ern1 and promoting M2 macrophage polarization

    doi: 10.1186/s13075-020-2146-x

    Figure Lengend Snippet: Ern1 was targeted by miR-124-3p. a Possible target genes of miR-124-3p predicted by bioinformatic analysis. b Expression of miR-124-3p in SCIRI tissues detected by RT-qPCR, * p

    Article Snippet: Exosome derived from BMMSCs carrying miR-124-3p modulates Ern1 and augments polarization of M2 macrophage in vivo With an aim to further verify the regulation of miR-124-3p on Ern1 and M2 macrophage polarization in SCIRI in vivo, SCIRI rats were injected with exosomes derived from BMMSCs carrying miR-124-3p.

    Techniques: Expressing, Quantitative RT-PCR

    hESC‐FN‐MSCs are capable of in vivo differentiating into cells of multiple lineages. a) Macroscopic appearance of adipose tissue newly formed in nude mice. Human extracellular matrix powders containing ASCs, BMMSCs, and hESC‐FN‐MSCs were implanted into the back of each nude mouse for 5 weeks. The formed tissue in the hESC‐FN‐MSC‐injected group was larger than those in the ASC‐ and BMMSC‐injected groups ( n = 4–5). b) The weight of adipose tissue formed in the group injected with hESC‐FN‐MSCs was compared with that formed in the groups injected with ASCs and BMMSCs. The formed tissue in the hESC‐FN‐MSC‐injected group was significantly heavier than that in the control (ECM‐only), but was not significantly different compared to the tissue in both the ASC‐ and BMMSC‐injected groups. n = 4, mean ± s.d., ns, not significant, and * P

    Journal: Advanced Science

    Article Title: Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin, Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin

    doi: 10.1002/advs.202001365

    Figure Lengend Snippet: hESC‐FN‐MSCs are capable of in vivo differentiating into cells of multiple lineages. a) Macroscopic appearance of adipose tissue newly formed in nude mice. Human extracellular matrix powders containing ASCs, BMMSCs, and hESC‐FN‐MSCs were implanted into the back of each nude mouse for 5 weeks. The formed tissue in the hESC‐FN‐MSC‐injected group was larger than those in the ASC‐ and BMMSC‐injected groups ( n = 4–5). b) The weight of adipose tissue formed in the group injected with hESC‐FN‐MSCs was compared with that formed in the groups injected with ASCs and BMMSCs. The formed tissue in the hESC‐FN‐MSC‐injected group was significantly heavier than that in the control (ECM‐only), but was not significantly different compared to the tissue in both the ASC‐ and BMMSC‐injected groups. n = 4, mean ± s.d., ns, not significant, and * P

    Article Snippet: To evaluate the population‐doubling time (PDT), human ASCs (ATCC), BMMSCs (ATCC), and hESC‐FN‐MSCs at passage 3 were counted and cultured at a starting number of 5 × 103 cells per well in 24‐well plates.

    Techniques: In Vivo, Mouse Assay, Injection

    Transcriptomic analysis elucidates the biological similarities between hESC‐FN‐MSCs and BMMSCs. a,b) Scatter plot and Venn diagram of significant differentially expressed genes (SDEs) versus different groups (BMMSCs vs hESCs, hESC‐FN‐MSCs vs hESCs, and hESC‐FN‐MSCs vs BMMSCs). hESC‐FN‐MSCs were more similar to BMMSCs than to hESCs. c) Heat map based on the log 2 of fragments per kilobase of transcript per million mapped reads (FPKM) for signature genes of pluripotency, mesoderm, endoderm, and ectoderm differentiation for hESCs, BMMSCs, and hESC‐FN‐MSCs samples. A heat map based on the signature genes of mesoderm differentiation showed similar upregulated gene expression patterns between hESC‐FN‐MSCs and BMMSCs. d) Hierarchical clustering of average gene expression profiles in hESCs, BMMSCs, and hESC‐FN‐MSCs. hESC‐FN‐MSCs were very similar to BMMSCs, but showed distinct differences from hESCs. e) PCA of the top 500 high variance genes within hESCs, BMMSCs, and hESC‐FN‐MSCs samples using the first two principal components. Each spot represents a single RNA‐seq. hESC‐FN‐MSCs were more similar to BMMSCs than to hESCs, n = 3. f) Significant GO terms for associated biological processes from sixfold up‐ and downregulated genes in hESC‐FN‐MSCs compared to hESCs and BMMSCs. Terms related to biological processes indicate high proliferation and cell replication. Bar charts present the six most significant terms in each category for each cell type sorted by mean −log10 ( P ‐values).

    Journal: Advanced Science

    Article Title: Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin, Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin

    doi: 10.1002/advs.202001365

    Figure Lengend Snippet: Transcriptomic analysis elucidates the biological similarities between hESC‐FN‐MSCs and BMMSCs. a,b) Scatter plot and Venn diagram of significant differentially expressed genes (SDEs) versus different groups (BMMSCs vs hESCs, hESC‐FN‐MSCs vs hESCs, and hESC‐FN‐MSCs vs BMMSCs). hESC‐FN‐MSCs were more similar to BMMSCs than to hESCs. c) Heat map based on the log 2 of fragments per kilobase of transcript per million mapped reads (FPKM) for signature genes of pluripotency, mesoderm, endoderm, and ectoderm differentiation for hESCs, BMMSCs, and hESC‐FN‐MSCs samples. A heat map based on the signature genes of mesoderm differentiation showed similar upregulated gene expression patterns between hESC‐FN‐MSCs and BMMSCs. d) Hierarchical clustering of average gene expression profiles in hESCs, BMMSCs, and hESC‐FN‐MSCs. hESC‐FN‐MSCs were very similar to BMMSCs, but showed distinct differences from hESCs. e) PCA of the top 500 high variance genes within hESCs, BMMSCs, and hESC‐FN‐MSCs samples using the first two principal components. Each spot represents a single RNA‐seq. hESC‐FN‐MSCs were more similar to BMMSCs than to hESCs, n = 3. f) Significant GO terms for associated biological processes from sixfold up‐ and downregulated genes in hESC‐FN‐MSCs compared to hESCs and BMMSCs. Terms related to biological processes indicate high proliferation and cell replication. Bar charts present the six most significant terms in each category for each cell type sorted by mean −log10 ( P ‐values).

    Article Snippet: To evaluate the population‐doubling time (PDT), human ASCs (ATCC), BMMSCs (ATCC), and hESC‐FN‐MSCs at passage 3 were counted and cultured at a starting number of 5 × 103 cells per well in 24‐well plates.

    Techniques: Expressing, RNA Sequencing Assay

    The MSC surface markers of hESC‐FN‐MSCs are similar to both of ASCs and BMMSCs, but hESC‐FN‐MSCs have unique cell behaviors and characteristics. a) Surface antigen profiling with FACS showed that hESC‐FN‐MSCs are similar to ASCs and BMMSCs. OCT4 is a pluripotent marker, while CD73, CD90, and CD105 are positive MSC markers, and CD31, CD34, and HLA‐DR are negative MSC markers. b,c) Upon measuring the cumulative cell number and population doubling time until 10 passages, hESC‐FN‐MSCs showed high proliferative capabilities with short doubling time compared to ASCs and BMMSCs. Interestingly, even at passage 10, hESC‐FN‐MSCs were able to maintain their short doubling time, but ASCs and BMMSCs drastically increased their doubling time. n = 4, mean ± s.d., ** P

    Journal: Advanced Science

    Article Title: Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin, Efficient Isolation and Enrichment of Mesenchymal Stem Cells from Human Embryonic Stem Cells by Utilizing the Interaction between Integrin α5β1 and Fibronectin

    doi: 10.1002/advs.202001365

    Figure Lengend Snippet: The MSC surface markers of hESC‐FN‐MSCs are similar to both of ASCs and BMMSCs, but hESC‐FN‐MSCs have unique cell behaviors and characteristics. a) Surface antigen profiling with FACS showed that hESC‐FN‐MSCs are similar to ASCs and BMMSCs. OCT4 is a pluripotent marker, while CD73, CD90, and CD105 are positive MSC markers, and CD31, CD34, and HLA‐DR are negative MSC markers. b,c) Upon measuring the cumulative cell number and population doubling time until 10 passages, hESC‐FN‐MSCs showed high proliferative capabilities with short doubling time compared to ASCs and BMMSCs. Interestingly, even at passage 10, hESC‐FN‐MSCs were able to maintain their short doubling time, but ASCs and BMMSCs drastically increased their doubling time. n = 4, mean ± s.d., ** P

    Article Snippet: To evaluate the population‐doubling time (PDT), human ASCs (ATCC), BMMSCs (ATCC), and hESC‐FN‐MSCs at passage 3 were counted and cultured at a starting number of 5 × 103 cells per well in 24‐well plates.

    Techniques: FACS, Marker

    Real time PCR analysis. Expression levels of mRNA depicted for (A) MZB1 (B) CXCL8, were measured in activated B cells with or without exosomes. The graphs show the results of 6 exosome batches (MZB1 exosome batches 6, 8, 10, 12, 9, 3, respectively; CXCL8 exosome batches 6, 9, 10, 8, 12, 3, respectively). GAPDH was used as internal controls for targeting mRNA expression, and data are expressed as the mean of triplicate samples ± S.E.

    Journal: Frontiers in Immunology

    Article Title: Mesenchymal Stromal Cell-Derived Exosomes Affect mRNA Expression and Function of B-Lymphocytes

    doi: 10.3389/fimmu.2018.03053

    Figure Lengend Snippet: Real time PCR analysis. Expression levels of mRNA depicted for (A) MZB1 (B) CXCL8, were measured in activated B cells with or without exosomes. The graphs show the results of 6 exosome batches (MZB1 exosome batches 6, 8, 10, 12, 9, 3, respectively; CXCL8 exosome batches 6, 9, 10, 8, 12, 3, respectively). GAPDH was used as internal controls for targeting mRNA expression, and data are expressed as the mean of triplicate samples ± S.E.

    Article Snippet: Characterization of bmMSC-Derived Exosomes Electron Microscopy Exosome pellets were loaded onto electron-microscope grids (Formvar/carbon film 200 Mesh, nickel; EMS FCF200-Ni) followed by washing, blocking, and incubating with primary antibodies (Purified Mouse Anti Human CD63, BD Pharmingen) and thereafter with gold-conjugated secondary Ab (12 nm Colloidal Gold-Affini Pure Goat Anti-Mouse IgG, EM Grade; Jackson Immuno Research).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Mesenchymal stem cell (MSC)-derived exosome characterization. (A) Electronic microscopy of MSC-derived exosomes. Right image—no primary antibody (Ab). Right image—exosome stained with gold-conjugates, which are secondary antibody to anti-CD63. (B) Zeta sizer measurement of MSC-derived exosomes. (A representative measurement of 4 extractions is shown). (C) Anti-CD63 labeled exosomes bound to aldehyde sulfate beads. Right image—a representative graph of the percentage of CD63 staining in eight exosome batches, isolated from 8 MSC donors. The difference in CD63 expression might reflect the variability between donors and protein expression in their MSCs.

    Journal: Frontiers in Immunology

    Article Title: Mesenchymal Stromal Cell-Derived Exosomes Affect mRNA Expression and Function of B-Lymphocytes

    doi: 10.3389/fimmu.2018.03053

    Figure Lengend Snippet: Mesenchymal stem cell (MSC)-derived exosome characterization. (A) Electronic microscopy of MSC-derived exosomes. Right image—no primary antibody (Ab). Right image—exosome stained with gold-conjugates, which are secondary antibody to anti-CD63. (B) Zeta sizer measurement of MSC-derived exosomes. (A representative measurement of 4 extractions is shown). (C) Anti-CD63 labeled exosomes bound to aldehyde sulfate beads. Right image—a representative graph of the percentage of CD63 staining in eight exosome batches, isolated from 8 MSC donors. The difference in CD63 expression might reflect the variability between donors and protein expression in their MSCs.

    Article Snippet: Characterization of bmMSC-Derived Exosomes Electron Microscopy Exosome pellets were loaded onto electron-microscope grids (Formvar/carbon film 200 Mesh, nickel; EMS FCF200-Ni) followed by washing, blocking, and incubating with primary antibodies (Purified Mouse Anti Human CD63, BD Pharmingen) and thereafter with gold-conjugated secondary Ab (12 nm Colloidal Gold-Affini Pure Goat Anti-Mouse IgG, EM Grade; Jackson Immuno Research).

    Techniques: Derivative Assay, Microscopy, Staining, Labeling, Isolation, Expressing

    Exosome suppression of activated peripheral blood mononuclear cell (PBMCS) and lymphocytes and internalization assays. (A) Thymidine incorporation assays. Mesenchymal stem cell (MSC)-derived exosomes co-cultured with: (1) PHA-activated PBMCs. The results summarize 3 experiments with 6 batches of exosomes derived from 0.5 × 10 6 MSCs and 9 batches of exosomes derived from 1 × 10 6 MSCs (batches no. 1–9). Control—Activated condition counts set to represent 100%. P

    Journal: Frontiers in Immunology

    Article Title: Mesenchymal Stromal Cell-Derived Exosomes Affect mRNA Expression and Function of B-Lymphocytes

    doi: 10.3389/fimmu.2018.03053

    Figure Lengend Snippet: Exosome suppression of activated peripheral blood mononuclear cell (PBMCS) and lymphocytes and internalization assays. (A) Thymidine incorporation assays. Mesenchymal stem cell (MSC)-derived exosomes co-cultured with: (1) PHA-activated PBMCs. The results summarize 3 experiments with 6 batches of exosomes derived from 0.5 × 10 6 MSCs and 9 batches of exosomes derived from 1 × 10 6 MSCs (batches no. 1–9). Control—Activated condition counts set to represent 100%. P

    Article Snippet: Characterization of bmMSC-Derived Exosomes Electron Microscopy Exosome pellets were loaded onto electron-microscope grids (Formvar/carbon film 200 Mesh, nickel; EMS FCF200-Ni) followed by washing, blocking, and incubating with primary antibodies (Purified Mouse Anti Human CD63, BD Pharmingen) and thereafter with gold-conjugated secondary Ab (12 nm Colloidal Gold-Affini Pure Goat Anti-Mouse IgG, EM Grade; Jackson Immuno Research).

    Techniques: Derivative Assay, Cell Culture

    Differentially expressed genes in activated B-lymphocytes, with or without incubation with exosomes. All 5 replicates of cells exposed to exosomes (batches no. 6, 7, 8), samples B5, B6, B8, B9, and B10 (green), were compared to the duplicate samples of cells without exosome exposure, B3 and B4 (blue). The normalized expression of significantly expressed genes, padj

    Journal: Frontiers in Immunology

    Article Title: Mesenchymal Stromal Cell-Derived Exosomes Affect mRNA Expression and Function of B-Lymphocytes

    doi: 10.3389/fimmu.2018.03053

    Figure Lengend Snippet: Differentially expressed genes in activated B-lymphocytes, with or without incubation with exosomes. All 5 replicates of cells exposed to exosomes (batches no. 6, 7, 8), samples B5, B6, B8, B9, and B10 (green), were compared to the duplicate samples of cells without exosome exposure, B3 and B4 (blue). The normalized expression of significantly expressed genes, padj

    Article Snippet: Characterization of bmMSC-Derived Exosomes Electron Microscopy Exosome pellets were loaded onto electron-microscope grids (Formvar/carbon film 200 Mesh, nickel; EMS FCF200-Ni) followed by washing, blocking, and incubating with primary antibodies (Purified Mouse Anti Human CD63, BD Pharmingen) and thereafter with gold-conjugated secondary Ab (12 nm Colloidal Gold-Affini Pure Goat Anti-Mouse IgG, EM Grade; Jackson Immuno Research).

    Techniques: Incubation, Expressing

    Immunoglobulin estimation by ELISA. Expression levels of immunoglobulin M were measured by ELISA, in cell culture supernatant of activated B-lymphocytes cultured with or without exosomes. The graphs show the results of 6 exosome batches (batches no. 9, 8, 3, 10, 6, 12), cultured with the same single B lymphocyte donor. Data are expressed as the mean of triplicate samples ± S.E.

    Journal: Frontiers in Immunology

    Article Title: Mesenchymal Stromal Cell-Derived Exosomes Affect mRNA Expression and Function of B-Lymphocytes

    doi: 10.3389/fimmu.2018.03053

    Figure Lengend Snippet: Immunoglobulin estimation by ELISA. Expression levels of immunoglobulin M were measured by ELISA, in cell culture supernatant of activated B-lymphocytes cultured with or without exosomes. The graphs show the results of 6 exosome batches (batches no. 9, 8, 3, 10, 6, 12), cultured with the same single B lymphocyte donor. Data are expressed as the mean of triplicate samples ± S.E.

    Article Snippet: Characterization of bmMSC-Derived Exosomes Electron Microscopy Exosome pellets were loaded onto electron-microscope grids (Formvar/carbon film 200 Mesh, nickel; EMS FCF200-Ni) followed by washing, blocking, and incubating with primary antibodies (Purified Mouse Anti Human CD63, BD Pharmingen) and thereafter with gold-conjugated secondary Ab (12 nm Colloidal Gold-Affini Pure Goat Anti-Mouse IgG, EM Grade; Jackson Immuno Research).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture

    Cytoskeleton (F-actin fibers) organization in bmMSCs after exposure to 0, 5, 10, and 20 μ g/mL ox-LDL for 6 hours.

    Journal: Mediators of Inflammation

    Article Title: Ox-LDL Promotes Migration and Adhesion of Bone Marrow-Derived Mesenchymal Stem Cells via Regulation of MCP-1 Expression

    doi: 10.1155/2013/691023

    Figure Lengend Snippet: Cytoskeleton (F-actin fibers) organization in bmMSCs after exposure to 0, 5, 10, and 20 μ g/mL ox-LDL for 6 hours.

    Article Snippet: Ox-LDL Mediates Reorganization of Cytoskeleton in bmMSCs Cytoskeleton has been known to regulate cell migration and adhesion [ ].

    Techniques:

    Characteristic of exosomes isolated from mouse BMMSCs. a Microvesicles in MSCs were analyzed by TEM. b Immunofluorescent staining of CD63 and CD9 in MSCs. c Differential centrifugation procedure for the isolation of exosomes from culture supernatants of MSCs (SN). d Isolated exosomes from control and IFN-γ-primed MSCs were analyzed by TEM. e Exosomes numbers isolated from control and IFN-γ-primed MSCs was analyzed by EXOCEP exosome quantitation kit. f The expression of CD81 and CD9 in exosomes from control and IFN-γ-primed MSCs were analyzed by western blotting. Scale bar a : 500 nm, b : 20 μm, and d : 100 nm. * P

    Journal: Cell Death & Disease

    Article Title: IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125b

    doi: 10.1038/s41419-020-02788-0

    Figure Lengend Snippet: Characteristic of exosomes isolated from mouse BMMSCs. a Microvesicles in MSCs were analyzed by TEM. b Immunofluorescent staining of CD63 and CD9 in MSCs. c Differential centrifugation procedure for the isolation of exosomes from culture supernatants of MSCs (SN). d Isolated exosomes from control and IFN-γ-primed MSCs were analyzed by TEM. e Exosomes numbers isolated from control and IFN-γ-primed MSCs was analyzed by EXOCEP exosome quantitation kit. f The expression of CD81 and CD9 in exosomes from control and IFN-γ-primed MSCs were analyzed by western blotting. Scale bar a : 500 nm, b : 20 μm, and d : 100 nm. * P

    Article Snippet: Exosome-depleted culture supernatant of BMMSCs was collected.

    Techniques: Isolation, Transmission Electron Microscopy, Staining, Centrifugation, Quantitation Assay, Expressing, Western Blot

    MiR-200b in HBM-exo targets Hmgb3 to attenuate the inflammatory injury of IECs. a TargetScan, miRWalk, and miRDB database predicted the microRNAs targeting the 3′ UTR of Hmgb3 . b QRT-PCR was used to detect the relative expression levels of miR-200b, miR-17, miR-139, miR-429, miR-191a, and miR-214a in TNF-α-exo, BM-exo, and HBM-exo groups (normalized by U6, fold change to TNF-α-exo, n = 5). c QRT-PCR was used to detect the relative expression of miR-200b in the Mock, TNF-α, TNF-α-exo, BM-exo, and HBM-exo groups (fold change relative to the Mock group, n = 4). d , e The plasmids HMGB3-WT (wild-type), HMGB3-MUT (mutant-type), positive control (Positive Con.) and negative control (Negative Con.) were constructed. Dual luciferase reporter assays were used to verify the targeted binding relationship between miR-200b and the 3′ UTR region of Hmgb3 in HEK293T cells and IEC-6s ( n = 3). f , g The protective effect of overexpression of miR-200b on inflammatory injury of IEC-6s. Transfection of miR-200b mimic and Negative Con., western blotting to verify the Mock group, Control group under TNF-α damage, and the Negative Con group. The relative expression levels of HMGB3, phosphorylated (p-) JNK, and other proteins in IEC-6s of the control and miR-200b mimic groups ( n = 3); h , i Interference with miR-200b expression to block the protective effect of HBM-exo on inflammatory IEC-6s. Transfection of an miR-200b inhibitor and Negative Con. After treatment, the relative expression levels of HMGB3 and p-JNK were detected in the model of IEC-6s protected by HBM-exo ( n = 3). j The relative expression levels of miR-200b in BMMSCs, HO-1/BMMSCs, TNF-α + BMMSCs, TNF-α + HO-1/BMMSCs cells, and exocrine bodies were detected (fold change to BMMSCs, n = 5). * P

    Journal: Cell Death & Disease

    Article Title: MiR-200b in heme oxygenase-1-modified bone marrow mesenchymal stem cell-derived exosomes alleviates inflammatory injury of intestinal epithelial cells by targeting high mobility group box 3

    doi: 10.1038/s41419-020-2685-8

    Figure Lengend Snippet: MiR-200b in HBM-exo targets Hmgb3 to attenuate the inflammatory injury of IECs. a TargetScan, miRWalk, and miRDB database predicted the microRNAs targeting the 3′ UTR of Hmgb3 . b QRT-PCR was used to detect the relative expression levels of miR-200b, miR-17, miR-139, miR-429, miR-191a, and miR-214a in TNF-α-exo, BM-exo, and HBM-exo groups (normalized by U6, fold change to TNF-α-exo, n = 5). c QRT-PCR was used to detect the relative expression of miR-200b in the Mock, TNF-α, TNF-α-exo, BM-exo, and HBM-exo groups (fold change relative to the Mock group, n = 4). d , e The plasmids HMGB3-WT (wild-type), HMGB3-MUT (mutant-type), positive control (Positive Con.) and negative control (Negative Con.) were constructed. Dual luciferase reporter assays were used to verify the targeted binding relationship between miR-200b and the 3′ UTR region of Hmgb3 in HEK293T cells and IEC-6s ( n = 3). f , g The protective effect of overexpression of miR-200b on inflammatory injury of IEC-6s. Transfection of miR-200b mimic and Negative Con., western blotting to verify the Mock group, Control group under TNF-α damage, and the Negative Con group. The relative expression levels of HMGB3, phosphorylated (p-) JNK, and other proteins in IEC-6s of the control and miR-200b mimic groups ( n = 3); h , i Interference with miR-200b expression to block the protective effect of HBM-exo on inflammatory IEC-6s. Transfection of an miR-200b inhibitor and Negative Con. After treatment, the relative expression levels of HMGB3 and p-JNK were detected in the model of IEC-6s protected by HBM-exo ( n = 3). j The relative expression levels of miR-200b in BMMSCs, HO-1/BMMSCs, TNF-α + BMMSCs, TNF-α + HO-1/BMMSCs cells, and exocrine bodies were detected (fold change to BMMSCs, n = 5). * P

    Article Snippet: Isolation, identification, and HO-1-overexpression modification of BMMSCs The rats used in our study were purchased from Vital River Company (Beijing, China).

    Techniques: Quantitative RT-PCR, Expressing, Mutagenesis, Positive Control, Negative Control, Construct, Luciferase, Binding Assay, Over Expression, Transfection, Western Blot, Blocking Assay

    Ho-1 gene overexpression changes the transcriptional expression profile of BMMSCs and improves immune regulation and stress tolerance abilities of BMMSCs. a – c HO-1/BMMSCs and GFP/BMMSCs were established by transfection of Adenovirus- Ho-1 and Adenovirus- Gfp . The relative expression levels of the mRNA and protein of HO-1 in HO-1/BMMSCs were verified using qRT-PCR ( a , fold change relative to BMMSCs, n = 4). Western blotting ( b , n = 3) and immunofluorescence ( c , n = 3). d The number of DEGs between HO-1/BMMSCs and GFP/BMMSCs. e A heatmap was used to represent the DEGs in HO-1/BMMSCs compared with GFP/BMMSCs. f , g Go analysis ( f ) and KEGG analysis ( g ) of the DEGs showed the biological function and signal pathways of BMMSCs regulation after Ho-1 modification. h QRT-PCR validation of the mRNA levels of selected DEGs to verify the results of RNA-sequencing, including Ifit1 , Akap2 , Nlrc5 , Rgs7 , Cdh1 , and Snip1 (fold change relative to BMMSCs, n = 3). * P

    Journal: Cell Death & Disease

    Article Title: MiR-200b in heme oxygenase-1-modified bone marrow mesenchymal stem cell-derived exosomes alleviates inflammatory injury of intestinal epithelial cells by targeting high mobility group box 3

    doi: 10.1038/s41419-020-2685-8

    Figure Lengend Snippet: Ho-1 gene overexpression changes the transcriptional expression profile of BMMSCs and improves immune regulation and stress tolerance abilities of BMMSCs. a – c HO-1/BMMSCs and GFP/BMMSCs were established by transfection of Adenovirus- Ho-1 and Adenovirus- Gfp . The relative expression levels of the mRNA and protein of HO-1 in HO-1/BMMSCs were verified using qRT-PCR ( a , fold change relative to BMMSCs, n = 4). Western blotting ( b , n = 3) and immunofluorescence ( c , n = 3). d The number of DEGs between HO-1/BMMSCs and GFP/BMMSCs. e A heatmap was used to represent the DEGs in HO-1/BMMSCs compared with GFP/BMMSCs. f , g Go analysis ( f ) and KEGG analysis ( g ) of the DEGs showed the biological function and signal pathways of BMMSCs regulation after Ho-1 modification. h QRT-PCR validation of the mRNA levels of selected DEGs to verify the results of RNA-sequencing, including Ifit1 , Akap2 , Nlrc5 , Rgs7 , Cdh1 , and Snip1 (fold change relative to BMMSCs, n = 3). * P

    Article Snippet: Isolation, identification, and HO-1-overexpression modification of BMMSCs The rats used in our study were purchased from Vital River Company (Beijing, China).

    Techniques: Over Expression, Expressing, Transfection, Quantitative RT-PCR, Western Blot, Immunofluorescence, Modification, RNA Sequencing Assay