Journal: Nephron. Clinical Practice
Article Title: Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins
Figure Lengend Snippet: Integrin small molecule inhibitors are as effective as Nintedanib in blocking ECM accumulation. Cells were cultured for 7 days in the presence of Nintedanib (1 µM), Pirfenidone (10 µM), Bindarit (10 µM), RS504393 (10 µM), Beraprost sodium (0.3 µM), IVA337 (3 µM), SB525334 (10 μM), Obeticholic acid (10 µM), Sarpogrelate hydrochloride (1 µM), TRAM34 (10 µM), Nilotinib (0.3 µM), Baricitinib (3 µM), Rapamycin (10 µM), FTY720 (3 µM), Pyridoxamine dihydrochloride (10 µM), BML-111 (10 µM), L3223 (10 µM), Enalapril maleate (10 µM), Emricasam (10 µM), CWHM12 (1 µM), C8 (10 µM), Cyclo (RGDyK) (0.04 µM), Cyclo (RGDfK) (0.1 µM), SB273005 (10 µM), Cilengitide (1 µM), DC9711 (10 µM). Cells were then lysed, and the ECM was fixed and stained using a total protein dye or specific antibodies. Graph shows the effect of each small molecule at the dose indicated (highest dose before effect on cell viability was observed) on the total ECM. DMSO control (0.1%) is also shown. Data are represented as fold change from the DMSO control and show the mean with 95% CIs from 2 independent experiments with 4 replicate wells per condition. * p
Article Snippet: For treatment with small molecule inhibitors, cells were incubated in the presence of nintedanib (Selleckchem), pirfenidone (Sigma-Aldrich), bindarit (Abcam), RS504393 (Sigma-Aldrich), beraprost sodium (Tocris Bioscience), IVA337 (UCB), obeticholic acid (Selleckchem), sarpogrelate hydrochloride (Sigma-Aldrich), TRAM34 (Sigma-Aldrich), nilotinib (Abcam), baricitinib (Selleckchem), rapamycin (Sigma-Aldrich), FTY720 (Sigma-Aldrich), pyridoxamine dihydrochloride (Sigma-Aldrich), BML-111 (Abcam), L3223 (Echelon Biosciences), enalapril maleate (Sigma-Aldrich), emricasam (Selleckchem), CWHM12 (UCB), C8 (UCB), Cyclo (RGDyK) (Selleckchem), Cyclo (RGDfK) (Selleckchem), SB273005 (Selleckchem), cilengitide (Selleckchem) and DC9711 (DC Chemicals) at the indicated concentrations.
Techniques: Blocking Assay, Cell Culture, Staining