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    Thermo Fisher bm mscs
    Bm Mscs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Lonza bm mscs
    Bm Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lonza human bone marrow derived mscs
    Human Bone Marrow Derived Mscs, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore bm mscs
    Role of the <t>AMPK-SIRT1</t> signaling pathway in KGN-mediated antioxidant effect. (a) The mRNA levels of SIRT1 in KGN-treated <t>BM-MSCs</t> were measured using real-time RT-PCR. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments. (b) The protein levels of SIRT1 were determined using Western blot assays. (c) Quantification of protein levels of SIRT1. (d) Activation of AMPK by KGN was determined using Western blot assays. (e–f) The phosphorylated levels of AMPK (e) and total expression of AMPK (f) in KGN-treated BM-MSCs were quantified. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments and of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p
    Bm Mscs, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bm mscs/product/Millipore
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    Role of the AMPK-SIRT1 signaling pathway in KGN-mediated antioxidant effect. (a) The mRNA levels of SIRT1 in KGN-treated BM-MSCs were measured using real-time RT-PCR. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments. (b) The protein levels of SIRT1 were determined using Western blot assays. (c) Quantification of protein levels of SIRT1. (d) Activation of AMPK by KGN was determined using Western blot assays. (e–f) The phosphorylated levels of AMPK (e) and total expression of AMPK (f) in KGN-treated BM-MSCs were quantified. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments and of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

    doi: 10.1155/2018/1368142

    Figure Lengend Snippet: Role of the AMPK-SIRT1 signaling pathway in KGN-mediated antioxidant effect. (a) The mRNA levels of SIRT1 in KGN-treated BM-MSCs were measured using real-time RT-PCR. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments. (b) The protein levels of SIRT1 were determined using Western blot assays. (c) Quantification of protein levels of SIRT1. (d) Activation of AMPK by KGN was determined using Western blot assays. (e–f) The phosphorylated levels of AMPK (e) and total expression of AMPK (f) in KGN-treated BM-MSCs were quantified. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments and of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Article Snippet: To investigate the role of SIRT1 in KGN-mediated antioxidant functions, BM-MSCs were treated with 10 mM NAM (Sigma-Aldrich) and 10−6 M KGN.

    Techniques: Quantitative RT-PCR, Western Blot, Activation Assay, Expressing

    The effect of KGN on osteogenic differentiation of BM-MSCs. Cells were induced toward osteogenic differentiation in the presence of 10 −8 M, 10 −7 M, and 10 −6 M KGN for 14 days. (a) Representative images of mineralized extracellular matrix stained by Alizarin Red S. Scale bar = 200 μ m. (b) Quantification of the stained mineral layers demonstrated that KGN increased calcium deposition in differentiated BM-MSCs. The stained mineral layers were treated with perchloric acid, and absorbance was measured at 420 nm. The values were normalized to the level of the CTRL group. (c–f) The mRNA levels of osteoblast-specific marker genes, including ALP (c), COL1A1 (d), RUNX2 (e), and BGLAP (f), were quantified with real-time RT-PCR using GAPDH for normalization. Values are the mean ± SEM of four independent experiments ( n = 4) in Alizarin Red S staining and real-time RT-PCR experiments. Statistically significant differences are indicated by ∗ where p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

    doi: 10.1155/2018/1368142

    Figure Lengend Snippet: The effect of KGN on osteogenic differentiation of BM-MSCs. Cells were induced toward osteogenic differentiation in the presence of 10 −8 M, 10 −7 M, and 10 −6 M KGN for 14 days. (a) Representative images of mineralized extracellular matrix stained by Alizarin Red S. Scale bar = 200 μ m. (b) Quantification of the stained mineral layers demonstrated that KGN increased calcium deposition in differentiated BM-MSCs. The stained mineral layers were treated with perchloric acid, and absorbance was measured at 420 nm. The values were normalized to the level of the CTRL group. (c–f) The mRNA levels of osteoblast-specific marker genes, including ALP (c), COL1A1 (d), RUNX2 (e), and BGLAP (f), were quantified with real-time RT-PCR using GAPDH for normalization. Values are the mean ± SEM of four independent experiments ( n = 4) in Alizarin Red S staining and real-time RT-PCR experiments. Statistically significant differences are indicated by ∗ where p

    Article Snippet: To investigate the role of SIRT1 in KGN-mediated antioxidant functions, BM-MSCs were treated with 10 mM NAM (Sigma-Aldrich) and 10−6 M KGN.

    Techniques: Staining, Marker, ALP Assay, Quantitative RT-PCR

    The effect of KGN on the expression and activity of superoxide dismutases. (a–b) The mRNA levels of SOD1 (a) and SOD2 (b) were measured using real-time RT-PCR. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments. (c) Treatment with KGN at 10 −7 M and 10 −6 M increased the activity of SOD in BM-MSCs. Values are the mean ± SEM of four independent experiments ( n = 4) in SOD activity experiments. (d) The increase in protein levels of SOD1 and SOD2 in KGN-treated BM-MSCs were confirmed using Western blot assays. (e–f) Quantification of protein levels of SOD1 (e) and SOD2 (f). Values are the mean ± SEM of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

    doi: 10.1155/2018/1368142

    Figure Lengend Snippet: The effect of KGN on the expression and activity of superoxide dismutases. (a–b) The mRNA levels of SOD1 (a) and SOD2 (b) were measured using real-time RT-PCR. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments. (c) Treatment with KGN at 10 −7 M and 10 −6 M increased the activity of SOD in BM-MSCs. Values are the mean ± SEM of four independent experiments ( n = 4) in SOD activity experiments. (d) The increase in protein levels of SOD1 and SOD2 in KGN-treated BM-MSCs were confirmed using Western blot assays. (e–f) Quantification of protein levels of SOD1 (e) and SOD2 (f). Values are the mean ± SEM of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Article Snippet: To investigate the role of SIRT1 in KGN-mediated antioxidant functions, BM-MSCs were treated with 10 mM NAM (Sigma-Aldrich) and 10−6 M KGN.

    Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Western Blot

    The inhibition of SIRT1 by NAM counteracted the effect of KGN on osteogenic differentiation of BM-MSCs. Cells were induced toward osteogenic differentiation in the presence of 10 −6 M KGN and 10 mM NAM for 14 days. (a) Representative images of mineralized extracellular matrix stained by Alizarin Red S. Scale bar = 200 μ m. (b) Quantification of the stained mineral layers demonstrated that NAM significantly decreased calcium deposition in differentiated BM-MSCs. The stained mineral layers were treated with perchloric acid, and absorbance was measured at 420 nm. The values were normalized to the level of the untreated cells. (c–f) The mRNA levels of osteoblast-specific marker genes, including ALP (c), COL1A1 (d), RUNX2 (e), and BGLAP (f), were quantified with real-time RT-PCR using GAPDH for normalization. Values are the mean ± SEM of four independent experiments ( n = 4) in Alizarin Red S staining and real-time RT-PCR experiments. Statistically significant differences are indicated by ∗ where p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

    doi: 10.1155/2018/1368142

    Figure Lengend Snippet: The inhibition of SIRT1 by NAM counteracted the effect of KGN on osteogenic differentiation of BM-MSCs. Cells were induced toward osteogenic differentiation in the presence of 10 −6 M KGN and 10 mM NAM for 14 days. (a) Representative images of mineralized extracellular matrix stained by Alizarin Red S. Scale bar = 200 μ m. (b) Quantification of the stained mineral layers demonstrated that NAM significantly decreased calcium deposition in differentiated BM-MSCs. The stained mineral layers were treated with perchloric acid, and absorbance was measured at 420 nm. The values were normalized to the level of the untreated cells. (c–f) The mRNA levels of osteoblast-specific marker genes, including ALP (c), COL1A1 (d), RUNX2 (e), and BGLAP (f), were quantified with real-time RT-PCR using GAPDH for normalization. Values are the mean ± SEM of four independent experiments ( n = 4) in Alizarin Red S staining and real-time RT-PCR experiments. Statistically significant differences are indicated by ∗ where p

    Article Snippet: To investigate the role of SIRT1 in KGN-mediated antioxidant functions, BM-MSCs were treated with 10 mM NAM (Sigma-Aldrich) and 10−6 M KGN.

    Techniques: Inhibition, Staining, Marker, ALP Assay, Quantitative RT-PCR

    The effect of KGN on the levels of CAT and GPX1. (a–b) The mRNA levels of CAT and GPX1 (b) were measured using real-time RT-PCR. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments. (c) Treatment with KGN showed no effect on the activity of CAT. Values are the mean ± SEM of four independent experiments ( n = 4) in CAT activity experiments. (d) The protein levels of CAT and GPX1 in KGN-treated BM-MSCs were determined using Western blot assays. (e–f) Quantification of protein levels of SOD1 (e) and SOD2 (f). Values are the mean ± SEM of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

    doi: 10.1155/2018/1368142

    Figure Lengend Snippet: The effect of KGN on the levels of CAT and GPX1. (a–b) The mRNA levels of CAT and GPX1 (b) were measured using real-time RT-PCR. Values are the mean ± SEM of four independent experiments ( n = 4) in real-time RT-PCR experiments. (c) Treatment with KGN showed no effect on the activity of CAT. Values are the mean ± SEM of four independent experiments ( n = 4) in CAT activity experiments. (d) The protein levels of CAT and GPX1 in KGN-treated BM-MSCs were determined using Western blot assays. (e–f) Quantification of protein levels of SOD1 (e) and SOD2 (f). Values are the mean ± SEM of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Article Snippet: To investigate the role of SIRT1 in KGN-mediated antioxidant functions, BM-MSCs were treated with 10 mM NAM (Sigma-Aldrich) and 10−6 M KGN.

    Techniques: Quantitative RT-PCR, Activity Assay, Western Blot

    The effect of KGN on cell proliferation and intracellular ROS of BM-MSCs. (a) BM-MSCs were treated with KGN at the concentrations of 10 −8 M, 10 −7 M, and 10 −6 M, and the cell proliferation was tested on days 1, 3, 5, and 7 using the CCK-8 assay. Values are the mean ± SEM of six independent experiments ( n = 6) in cell proliferation assays. (b) Intracellular ROS of KGN-treated BM-MSCs were determined by flow cytometry. (c) Quantification data showed that treatments with KGN attenuated the levels of intracellular ROS in BM-MSCs. Values are the mean ± SEM of four independent experiments ( n = 4) in ROS assays. Untreated cells served as the CTRL group, and cells treated with DMSO served as the vehicle control. Statistically significant differences are indicated by ∗ where p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

    doi: 10.1155/2018/1368142

    Figure Lengend Snippet: The effect of KGN on cell proliferation and intracellular ROS of BM-MSCs. (a) BM-MSCs were treated with KGN at the concentrations of 10 −8 M, 10 −7 M, and 10 −6 M, and the cell proliferation was tested on days 1, 3, 5, and 7 using the CCK-8 assay. Values are the mean ± SEM of six independent experiments ( n = 6) in cell proliferation assays. (b) Intracellular ROS of KGN-treated BM-MSCs were determined by flow cytometry. (c) Quantification data showed that treatments with KGN attenuated the levels of intracellular ROS in BM-MSCs. Values are the mean ± SEM of four independent experiments ( n = 4) in ROS assays. Untreated cells served as the CTRL group, and cells treated with DMSO served as the vehicle control. Statistically significant differences are indicated by ∗ where p

    Article Snippet: To investigate the role of SIRT1 in KGN-mediated antioxidant functions, BM-MSCs were treated with 10 mM NAM (Sigma-Aldrich) and 10−6 M KGN.

    Techniques: CCK-8 Assay, Flow Cytometry, Cytometry

    The inhibition of SIRT1 by NAM reversed the KGN-mediated antioxidant effect on BM-MSCs. To inhibit the activity of SIRT1, BM-MSCs were treated with 10 mM NAM with or without the supplementation of 10 −6 M KGN. (a) Intracellular ROS of KGN-treated and NAM-treated BM-MSCs were determined using flow cytometry. (b) Quantification data showed that NAM significantly increased the levels of intracellular ROS in BM-MSCs. (c) The protein levels of SIRT1 and AMPK and the phosphorylated levels of AMPK were determined using Western blot assays. (d) The protein levels of intracellular antioxidant enzymes, including SOD1, SOD2, CAT, and GPX1, were determined using Western blot assays. Values are the mean ± SEM of four independent experiments ( n = 4) in ROS assays and of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Upregulation of SIRT1 by Kartogenin Enhances Antioxidant Functions and Promotes Osteogenesis in Human Mesenchymal Stem Cells

    doi: 10.1155/2018/1368142

    Figure Lengend Snippet: The inhibition of SIRT1 by NAM reversed the KGN-mediated antioxidant effect on BM-MSCs. To inhibit the activity of SIRT1, BM-MSCs were treated with 10 mM NAM with or without the supplementation of 10 −6 M KGN. (a) Intracellular ROS of KGN-treated and NAM-treated BM-MSCs were determined using flow cytometry. (b) Quantification data showed that NAM significantly increased the levels of intracellular ROS in BM-MSCs. (c) The protein levels of SIRT1 and AMPK and the phosphorylated levels of AMPK were determined using Western blot assays. (d) The protein levels of intracellular antioxidant enzymes, including SOD1, SOD2, CAT, and GPX1, were determined using Western blot assays. Values are the mean ± SEM of four independent experiments ( n = 4) in ROS assays and of three independent experiments ( n = 3) in Western blot assays. Statistically significant differences are indicated by ∗ where p

    Article Snippet: To investigate the role of SIRT1 in KGN-mediated antioxidant functions, BM-MSCs were treated with 10 mM NAM (Sigma-Aldrich) and 10−6 M KGN.

    Techniques: Inhibition, Activity Assay, Flow Cytometry, Cytometry, Western Blot