block-it green fluorescent oligo Search Results


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  • 99
    Thermo Fisher green block it fluorescent oligo
    Green Block It Fluorescent Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/green block it fluorescent oligo/product/Thermo Fisher
    Average 99 stars, based on 154 article reviews
    Price from $9.99 to $1999.99
    green block it fluorescent oligo - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher block it green fluorescent oligo
    Block It Green Fluorescent Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/block it green fluorescent oligo/product/Thermo Fisher
    Average 97 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    block it green fluorescent oligo - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

    98
    Thermo Fisher control sirna
    Repressive H3K27 trimethylation is reduced in <t>Dnmt1</t> -deficient cells. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with control (A, C) or Dnmt1 <t>siRNA</t> (B, D) and stained for H3K7me3, presented as fluorescence intensity in black/white code and as thermal color-code (thermal LUT). The mean grey value relative to control is quantified in E (n = 34 POA and 118 N2a cells for ctrl siRNA, n = 32 POA and 144 N2a cells for Dnmt1 siRNA). (F, G) Protein levels of DNMT1, EZH2 and H3K27me3 in control and Dnmt1 siRNA-treated N2a cells analyzed with Western blot (G), normalized to ACTB and quantified in relation to control (G; five individual experiments). (H) Co-immunoprecipitation with a DNMT1-specific antibody revealed a protein-protein interaction with EZH2 in E16 POA and N2a cell samples analyzed by Western blot (three individual experiments, two animals per experiment for POA cells). ‘n’ refers to the number of analyzed cells. *P
    Control Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 15990 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control sirna/product/Thermo Fisher
    Average 98 stars, based on 15990 article reviews
    Price from $9.99 to $1999.99
    control sirna - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    85
    Thermo Fisher blockit alexa fluor red fluorescent oligo
    Repressive H3K27 trimethylation is reduced in <t>Dnmt1</t> -deficient cells. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with control (A, C) or Dnmt1 <t>siRNA</t> (B, D) and stained for H3K7me3, presented as fluorescence intensity in black/white code and as thermal color-code (thermal LUT). The mean grey value relative to control is quantified in E (n = 34 POA and 118 N2a cells for ctrl siRNA, n = 32 POA and 144 N2a cells for Dnmt1 siRNA). (F, G) Protein levels of DNMT1, EZH2 and H3K27me3 in control and Dnmt1 siRNA-treated N2a cells analyzed with Western blot (G), normalized to ACTB and quantified in relation to control (G; five individual experiments). (H) Co-immunoprecipitation with a DNMT1-specific antibody revealed a protein-protein interaction with EZH2 in E16 POA and N2a cell samples analyzed by Western blot (three individual experiments, two animals per experiment for POA cells). ‘n’ refers to the number of analyzed cells. *P
    Blockit Alexa Fluor Red Fluorescent Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blockit alexa fluor red fluorescent oligo/product/Thermo Fisher
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    blockit alexa fluor red fluorescent oligo - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Repressive H3K27 trimethylation is reduced in Dnmt1 -deficient cells. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with control (A, C) or Dnmt1 siRNA (B, D) and stained for H3K7me3, presented as fluorescence intensity in black/white code and as thermal color-code (thermal LUT). The mean grey value relative to control is quantified in E (n = 34 POA and 118 N2a cells for ctrl siRNA, n = 32 POA and 144 N2a cells for Dnmt1 siRNA). (F, G) Protein levels of DNMT1, EZH2 and H3K27me3 in control and Dnmt1 siRNA-treated N2a cells analyzed with Western blot (G), normalized to ACTB and quantified in relation to control (G; five individual experiments). (H) Co-immunoprecipitation with a DNMT1-specific antibody revealed a protein-protein interaction with EZH2 in E16 POA and N2a cell samples analyzed by Western blot (three individual experiments, two animals per experiment for POA cells). ‘n’ refers to the number of analyzed cells. *P

    Journal: Epigenetics

    Article Title: DNMT1 modulates interneuron morphology by regulating Pak6 expression through crosstalk with histone modifications

    doi: 10.1080/15592294.2018.1475980

    Figure Lengend Snippet: Repressive H3K27 trimethylation is reduced in Dnmt1 -deficient cells. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with control (A, C) or Dnmt1 siRNA (B, D) and stained for H3K7me3, presented as fluorescence intensity in black/white code and as thermal color-code (thermal LUT). The mean grey value relative to control is quantified in E (n = 34 POA and 118 N2a cells for ctrl siRNA, n = 32 POA and 144 N2a cells for Dnmt1 siRNA). (F, G) Protein levels of DNMT1, EZH2 and H3K27me3 in control and Dnmt1 siRNA-treated N2a cells analyzed with Western blot (G), normalized to ACTB and quantified in relation to control (G; five individual experiments). (H) Co-immunoprecipitation with a DNMT1-specific antibody revealed a protein-protein interaction with EZH2 in E16 POA and N2a cell samples analyzed by Western blot (three individual experiments, two animals per experiment for POA cells). ‘n’ refers to the number of analyzed cells. *P

    Article Snippet: Mouse Dnmt1 siRNA oligos (50 nM) or mouse Pak6 siRNA oligos (50 nM), containing a pool of at least three target-specific 19–25 nt siRNAs to knockdown gene expression ( Dnmt1 siRNA sc-35,203; Pak6 siRNA sc-44,879; Santa Cruz Biotechnology) or 50 nM control siRNA (BLOCK-iT Alexa Fluor red/green fluorescent oligo; Invitrogen) were applied for 5 h in antibiotic-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) at 37°C, 5% CO2 , and 95% humidity.

    Techniques: Staining, Fluorescence, Western Blot, Immunoprecipitation

    Dnmt1 -depletion causes a reduced association of trimethylated H3K27 with Pak6 regulatory regions. (A) Schematic illustration of the Pak6 gene locus with promoter (red), promoter flanking (light red), enhancer (yellow) and non-regulatory (white) sites according to UCSC genome browser showing specific DNA primer positions (#1, #2, #3) in regulatory (#1, #2) and non-regulatory (#3) gene regions. (B, C) ChIP-analysis of control and Dnmt1 siRNA-treated N2a cells with a H3K27me3-specific antibody shows the association of H3K27me3 to the indicated primer locations (#1–3 in A) in the Pak6 gene (B), analyzed by quantitative PCR and normalized to the amount of input (C; three individual experiments with four technical replicates each). IgG controls indicate the amount of non-specifically bound DNA. ***P

    Journal: Epigenetics

    Article Title: DNMT1 modulates interneuron morphology by regulating Pak6 expression through crosstalk with histone modifications

    doi: 10.1080/15592294.2018.1475980

    Figure Lengend Snippet: Dnmt1 -depletion causes a reduced association of trimethylated H3K27 with Pak6 regulatory regions. (A) Schematic illustration of the Pak6 gene locus with promoter (red), promoter flanking (light red), enhancer (yellow) and non-regulatory (white) sites according to UCSC genome browser showing specific DNA primer positions (#1, #2, #3) in regulatory (#1, #2) and non-regulatory (#3) gene regions. (B, C) ChIP-analysis of control and Dnmt1 siRNA-treated N2a cells with a H3K27me3-specific antibody shows the association of H3K27me3 to the indicated primer locations (#1–3 in A) in the Pak6 gene (B), analyzed by quantitative PCR and normalized to the amount of input (C; three individual experiments with four technical replicates each). IgG controls indicate the amount of non-specifically bound DNA. ***P

    Article Snippet: Mouse Dnmt1 siRNA oligos (50 nM) or mouse Pak6 siRNA oligos (50 nM), containing a pool of at least three target-specific 19–25 nt siRNAs to knockdown gene expression ( Dnmt1 siRNA sc-35,203; Pak6 siRNA sc-44,879; Santa Cruz Biotechnology) or 50 nM control siRNA (BLOCK-iT Alexa Fluor red/green fluorescent oligo; Invitrogen) were applied for 5 h in antibiotic-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) at 37°C, 5% CO2 , and 95% humidity.

    Techniques: Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

    Enhanced Pak6 expression induced by EZH2 inhibition results in increased morphological complexity. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with DMSO (A, C) or DZNep (B, D) and stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). Quantification of cell morphology is shown in (E) as complexity index relative to DMSO controls. The complexity index is a product of primary neurite number, the branch point number of the longest process and the ratio between longest process length and the mean length of all other neurites. (E; n = 52 POA and 85 N2a cells for DMSO, n = 69 POA and 93 N2a cells for DZNep). (F-J) Representative microphotographs of dissociated E16 (+ 2 div) POA cells treated either with DMSO (F, H) or DZNep (G, I) in addition to control (F, G) or Pak6 siRNA (H, I), then stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). The complexity index is quantified in (J) relative to DMSO + ctrl siRNA cell parameters (n = 39 POA cells for DMSO + ctrl siRNA, n = 43 POA cells for DZNep + ctrl siRNA, n = 51 POA cells for DMSO + Pak6 siRNA, n = 68 POA cells for DZNep + Pak6 siRNA). ‘n’ refers to the number of analyzed cells. **P

    Journal: Epigenetics

    Article Title: DNMT1 modulates interneuron morphology by regulating Pak6 expression through crosstalk with histone modifications

    doi: 10.1080/15592294.2018.1475980

    Figure Lengend Snippet: Enhanced Pak6 expression induced by EZH2 inhibition results in increased morphological complexity. (A-E) Representative microphotographs of dissociated E16 (+ 2 DIV) POA cells (A, B) and N2a cells (C, D) treated either with DMSO (A, C) or DZNep (B, D) and stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). Quantification of cell morphology is shown in (E) as complexity index relative to DMSO controls. The complexity index is a product of primary neurite number, the branch point number of the longest process and the ratio between longest process length and the mean length of all other neurites. (E; n = 52 POA and 85 N2a cells for DMSO, n = 69 POA and 93 N2a cells for DZNep). (F-J) Representative microphotographs of dissociated E16 (+ 2 div) POA cells treated either with DMSO (F, H) or DZNep (G, I) in addition to control (F, G) or Pak6 siRNA (H, I), then stained against TUBB3 (green channel) with a specific antibody, ACTB (red channel) with fluorescently labeled phalloidin and DAPI (blue channel). The complexity index is quantified in (J) relative to DMSO + ctrl siRNA cell parameters (n = 39 POA cells for DMSO + ctrl siRNA, n = 43 POA cells for DZNep + ctrl siRNA, n = 51 POA cells for DMSO + Pak6 siRNA, n = 68 POA cells for DZNep + Pak6 siRNA). ‘n’ refers to the number of analyzed cells. **P

    Article Snippet: Mouse Dnmt1 siRNA oligos (50 nM) or mouse Pak6 siRNA oligos (50 nM), containing a pool of at least three target-specific 19–25 nt siRNAs to knockdown gene expression ( Dnmt1 siRNA sc-35,203; Pak6 siRNA sc-44,879; Santa Cruz Biotechnology) or 50 nM control siRNA (BLOCK-iT Alexa Fluor red/green fluorescent oligo; Invitrogen) were applied for 5 h in antibiotic-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) at 37°C, 5% CO2 , and 95% humidity.

    Techniques: Expressing, Inhibition, Staining, Labeling