black 96-well plate Search Results


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  • 99
    Thermo Fisher nunc black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Nunc Black 96 Well Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc black 96 well plate/product/Thermo Fisher
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    nunc black 96 well plate - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Millipore black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Millipore
    Average 99 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    99/100 stars
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    94
    Becton Dickinson black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Becton Dickinson
    Average 94 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    94/100 stars
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    94
    BRAND GMBH black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by BRAND GMBH, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/BRAND GMBH
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    94/100 stars
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    99
    Corning Life Sciences black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 99/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Corning Life Sciences
    Average 99 stars, based on 328 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
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    96
    Fisher Scientific black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 96/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Fisher Scientific
    Average 96 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    96/100 stars
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    99
    Greiner Bio black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 99/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Greiner Bio
    Average 99 stars, based on 277 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
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    95
    PerkinElmer black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 95/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/PerkinElmer
    Average 95 stars, based on 167 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
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    93
    USA Scientific Inc black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by USA Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/USA Scientific Inc
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    93/100 stars
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    87
    E&K Scientific black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by E&K Scientific, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/E&K Scientific
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    87/100 stars
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    96
    Molecular Devices LLC black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 96/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Molecular Devices LLC
    Average 96 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    96/100 stars
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    94
    Bio-Rad black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Bio-Rad
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    94/100 stars
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    92
    Avantor black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Avantor
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    92/100 stars
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    92
    Cayman Chemical black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/black 96 well plate/product/Cayman Chemical
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    black 96 well plate - by Bioz Stars, 2020-04
    92/100 stars
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    87
    Thermo Fisher microfluor black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Microfluor Black 96 Well Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microfluor black 96 well plate/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microfluor black 96 well plate - by Bioz Stars, 2020-04
    87/100 stars
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    85
    Corning Life Sciences sterile black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Sterile Black 96 Well Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile black 96 well plate/product/Corning Life Sciences
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sterile black 96 well plate - by Bioz Stars, 2020-04
    85/100 stars
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    94
    Greiner Bio solid black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Solid Black 96 Well Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/solid black 96 well plate/product/Greiner Bio
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    solid black 96 well plate - by Bioz Stars, 2020-04
    94/100 stars
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    94
    SPL Life Sciences opaque black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Opaque Black 96 Well Plate, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/opaque black 96 well plate/product/SPL Life Sciences
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    opaque black 96 well plate - by Bioz Stars, 2020-04
    94/100 stars
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    92
    Avantor polystyrene black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Polystyrene Black 96 Well Plate, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences polystyrene black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Polystyrene Black 96 Well Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioTek Instruments black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 94/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BMG Labtech black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by BMG Labtech, used in various techniques. Bioz Stars score: 97/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tecan Systems black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 95/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SPL Life Sciences black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Berthold Technologies black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Black 96 Well Plate, supplied by Berthold Technologies, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Greiner Bio opaque black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
    Opaque Black 96 Well Plate, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences 3694 black 96 well plate
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
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    LabTech Inc black 96 well plate spectrofluorometer
    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)
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    Image Search Results


    Schematic protocol for  R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to  R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In  R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different  Agrobacterium  and then co-infiltrated into the leaves of  N. benthamiana  in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at  JXB  online.)

    Journal: Journal of Experimental Botany

    Article Title: A reversible Renilla luciferase protein complementation assay for rapid identification of protein–protein interactions reveals the existence of an interaction network involved in xyloglucan biosynthesis in the plant Golgi apparatus

    doi: 10.1093/jxb/eru401

    Figure Lengend Snippet: Schematic protocol for R luc-PCA. Genes of interest (GOIs) are PCR amplified and recombined into pDONR TM /Zeo vector by BP cloning (BP). The GOI-containing entry clone can be recombined by LR cloning (LR) to insert GOI into desired destination vectors, which here is the Gateway-compatible ph R luc[F1] and ph R luc[F2] belonging to R luc-PCA. Alternatively, GOI can be recombined into other destination vectors, e.g. Gateway-compatible DUALmembrane vectors containing the amino terminal ubiquitin fragment (NubG) and the carboxy terminal ubiquitin fragment fused to an artificial transcription factor (TF–Cub). In R luc-PCA, ph R luc[F1]- and ph R luc[F2]-fused GOIs are individually introduced into different Agrobacterium and then co-infiltrated into the leaves of N. benthamiana in desired combinations to test PPIs. The assay is performed 3–4 d post infiltration by harvesting three leaf discs and transferring to tubes containing 200 µl assay buffer and a chrome ball. Leaf discs are macerated and 100 µl is transferred into a black 96-well plate. Bioluminescence upon addition of coelenterazine-h is monitored in a plate luminometer. (This figure is available in colour at JXB online.)

    Article Snippet: Of each sample, 100 µl was transferred to a Nunc black 96-well plate (Thermo Scientific, Rockford, IL, USA).

    Techniques: Polymerase Chain Reaction, Amplification, Plasmid Preparation, Clone Assay, Transferring

    The rPrP–LMWHep complex formed at pH 5.8 is resistant to conversion. For this assay, 98 μl of fresh RT-QuIC buffer (10 mM phosphate buffer, pH 7.4; 130 mM NaCl; 0.1 mg/ml rPrP  23–231 –LMWHep; 10 mM ThT; and 10 mM EDTA) was loaded into the wells of a black 96-well plate with a clear bottom. LMWHep, at a final concentration of 10 μM, was added to rPrP in 10 mM phosphate buffer containing 130 mM NaCl at pH 7.0 ( A ) or 5.8 ( B ). The reactions were seeded with 2 μl of a 10 −7  dilution of mouse BH. NBH, normal BH. The average ThT fluorescence from a set of quadruplicate wells is reported on the vertical axis.

    Journal: The FASEB Journal

    Article Title: Heparin binding confers prion stability and impairs its aggregation

    doi: 10.1096/fj.13-246777

    Figure Lengend Snippet: The rPrP–LMWHep complex formed at pH 5.8 is resistant to conversion. For this assay, 98 μl of fresh RT-QuIC buffer (10 mM phosphate buffer, pH 7.4; 130 mM NaCl; 0.1 mg/ml rPrP 23–231 –LMWHep; 10 mM ThT; and 10 mM EDTA) was loaded into the wells of a black 96-well plate with a clear bottom. LMWHep, at a final concentration of 10 μM, was added to rPrP in 10 mM phosphate buffer containing 130 mM NaCl at pH 7.0 ( A ) or 5.8 ( B ). The reactions were seeded with 2 μl of a 10 −7 dilution of mouse BH. NBH, normal BH. The average ThT fluorescence from a set of quadruplicate wells is reported on the vertical axis.

    Article Snippet: Briefly, 98 μl of fresh RT-QuIC buffer (10 mM phosphate buffer, pH 7.4; 130 mM NaCl; 0.1 mg/ml rPrP; 10 mM ThT; and 10 mM EDTA) was loaded into the wells of a black 96-well plate with a clear bottom (Nalge Nunc International, Penfield, NY, USA).

    Techniques: Concentration Assay, Fluorescence