bl21 de3 competent cells Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore bl 21 de3 competent cells
    Western blotting analysis of the IL6 expression with reduced IPTG concentrations. (S) soluble and (I) insoluble fraction of IL6, expressed with either 1 mM or 0.1 mM IPTG at 22°C in the cytoplasm of the E. coli <t>BL21</t> strain with and without the concomitant overexpression of endogenous cytoplasmic chaperones or in fusion to GST.
    Bl 21 De3 Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl 21 de3 competent cells/product/Millipore
    Average 99 stars, based on 519 article reviews
    Price from $9.99 to $1999.99
    bl 21 de3 competent cells - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Promega bl21 de3 competent cells
    Western blotting analysis of the IL6 expression with reduced IPTG concentrations. (S) soluble and (I) insoluble fraction of IL6, expressed with either 1 mM or 0.1 mM IPTG at 22°C in the cytoplasm of the E. coli <t>BL21</t> strain with and without the concomitant overexpression of endogenous cytoplasmic chaperones or in fusion to GST.
    Bl21 De3 Competent Cells, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 competent cells/product/Promega
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 competent cells - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Western blotting analysis of the IL6 expression with reduced IPTG concentrations. (S) soluble and (I) insoluble fraction of IL6, expressed with either 1 mM or 0.1 mM IPTG at 22°C in the cytoplasm of the E. coli BL21 strain with and without the concomitant overexpression of endogenous cytoplasmic chaperones or in fusion to GST.

    Journal: PLoS ONE

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli

    doi: 10.1371/journal.pone.0054933

    Figure Lengend Snippet: Western blotting analysis of the IL6 expression with reduced IPTG concentrations. (S) soluble and (I) insoluble fraction of IL6, expressed with either 1 mM or 0.1 mM IPTG at 22°C in the cytoplasm of the E. coli BL21 strain with and without the concomitant overexpression of endogenous cytoplasmic chaperones or in fusion to GST.

    Article Snippet: Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes.

    Techniques: Western Blot, Expressing, Over Expression

    Western blotting analysis of the IL6-expressing E. coli strains BL21 and Origami 2. (S) soluble and (I) insoluble fraction of IL6.

    Journal: PLoS ONE

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli

    doi: 10.1371/journal.pone.0054933

    Figure Lengend Snippet: Western blotting analysis of the IL6-expressing E. coli strains BL21 and Origami 2. (S) soluble and (I) insoluble fraction of IL6.

    Article Snippet: Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes.

    Techniques: Western Blot, Expressing

    Western blotting analysis of the IL6 expression in the cytoplasm and periplasm of E. coli BL21. (S) soluble and (I) insoluble fraction of IL6, expressed with and without the concomitant overexpression of endogenous cytoplasmic chaperones DnaK, DnaJ, GrpE, GroES, GroEL (C-Chaperones) or periplasmic chaperones DsbA, DsbC, SurA, FkpA (P-Chaperones) at 37°C and 22°C, respectively. Additionally, IL6 was expressed fused to GST in the cytoplasm.

    Journal: PLoS ONE

    Article Title: Recombinant Production of Human Interleukin 6 in Escherichia coli

    doi: 10.1371/journal.pone.0054933

    Figure Lengend Snippet: Western blotting analysis of the IL6 expression in the cytoplasm and periplasm of E. coli BL21. (S) soluble and (I) insoluble fraction of IL6, expressed with and without the concomitant overexpression of endogenous cytoplasmic chaperones DnaK, DnaJ, GrpE, GroES, GroEL (C-Chaperones) or periplasmic chaperones DsbA, DsbC, SurA, FkpA (P-Chaperones) at 37°C and 22°C, respectively. Additionally, IL6 was expressed fused to GST in the cytoplasm.

    Article Snippet: Competent BL21 (DE3) and Origami 2 (DE3) cells (Novagen) were transformed by electroporation with the different pET-vectors for expression of the target genes.

    Techniques: Western Blot, Expressing, Over Expression

    Characterization of the Anti- M. tuberculosis MutT Antisera A. Titers of antisera prepared from the same rabbit immunized with purified M. tuberculosis MutT are examined using total lysate of E. coli strain BL21 (DE3) harboring pET28a (+)-mutT. The batches of antisera were collected on the 6th (1st) and the 7th day (2nd) after the 1st and the 2nd boost immunization, respectively. A prominent signal of the recombinant M. tuberculosis MutT protein was still present (arrowhead) even at high dilution factors. B. The same blots were stripped and probed with anti-His antibody (Clontech, 1:5000) to mark the position of the recombinant M. tuberculosis MutT protein.

    Journal: Jundishapur Journal of Microbiology

    Article Title: A Molecular Biological and Biochemical Investigation on Mycobacterium tuberculosis MutT Protein

    doi: 10.5812/jjm.9367

    Figure Lengend Snippet: Characterization of the Anti- M. tuberculosis MutT Antisera A. Titers of antisera prepared from the same rabbit immunized with purified M. tuberculosis MutT are examined using total lysate of E. coli strain BL21 (DE3) harboring pET28a (+)-mutT. The batches of antisera were collected on the 6th (1st) and the 7th day (2nd) after the 1st and the 2nd boost immunization, respectively. A prominent signal of the recombinant M. tuberculosis MutT protein was still present (arrowhead) even at high dilution factors. B. The same blots were stripped and probed with anti-His antibody (Clontech, 1:5000) to mark the position of the recombinant M. tuberculosis MutT protein.

    Article Snippet: Bacterial Strains, Plasmids, and Antibodies E. coli isogenic strain MK602 (leu+ mutT ) was provided by Dr. M. Sekiguchi , and BL21 (DE3) was purchased from Novagen (Madison, WI).

    Techniques: Purification, Recombinant

    Overexpression of the Recombinant M. tuberculosis MutT Protein in BL21 (DE3) Plasmid pET28a (+)-mutT was overexpressed in E. coli strain BL21 (DE3) upon IPTG induction to produce the recombinant M. tuberculosis MutT protein. Lysates of bacteria were fractionated in a SDS-PAGE gel, and stained with Coomassie Blue. The recombinant M. tuberculosis MutT protein of 17.3 kD was found in the fractions.

    Journal: Jundishapur Journal of Microbiology

    Article Title: A Molecular Biological and Biochemical Investigation on Mycobacterium tuberculosis MutT Protein

    doi: 10.5812/jjm.9367

    Figure Lengend Snippet: Overexpression of the Recombinant M. tuberculosis MutT Protein in BL21 (DE3) Plasmid pET28a (+)-mutT was overexpressed in E. coli strain BL21 (DE3) upon IPTG induction to produce the recombinant M. tuberculosis MutT protein. Lysates of bacteria were fractionated in a SDS-PAGE gel, and stained with Coomassie Blue. The recombinant M. tuberculosis MutT protein of 17.3 kD was found in the fractions.

    Article Snippet: Bacterial Strains, Plasmids, and Antibodies E. coli isogenic strain MK602 (leu+ mutT ) was provided by Dr. M. Sekiguchi , and BL21 (DE3) was purchased from Novagen (Madison, WI).

    Techniques: Over Expression, Recombinant, Plasmid Preparation, SDS Page, Staining