bl21 Thermo Fisher Search Results


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  • 99
    Thermo Fisher bl21 de3 competent cells
    Bl21 De3 Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher e coli bl21 ai thermo fisher
    E Coli Bl21 Ai Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher bl21
    SDS-PAGE (12.5% polyacrylamide gel) of rATB. Lanes: 1: molecular size markers; 2: E. coli <t>BL21</t> (pET28arATB) prior to induction; 3: E. coli BL21 (pET28arATB) after induction; 4: 5 μ g denatured rATB in inclusion bodies; 5: 1 μ g refolded soluble rATB.
    Bl21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher bl21 ai
    Physiological effects of rate of membrane protein production. CX21-GFP was produced by rapid, restrained, and basal expression in <t>BL21-AI</t> cells. A: Biomass production. Growth was monitored via OD 600 nm of culture. B: Cell viability analysis using the
    Bl21 Ai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher bl21 de3 plys
    Expression and purification of fusion protein. <t>1—pET16b–scFv-DAF/BL21</t> (DE3) <t>plyss</t> before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.
    Bl21 De3 Plys, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher bl21 star
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 Star, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 991 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher bl21 plyss
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 Plyss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher coli bl21 al
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Coli Bl21 Al, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher prsetb
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Prsetb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1007 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher bl21 de3 rosetta
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 De3 Rosetta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bl21 gold
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bl21 codonplus
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 Codonplus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher bl21 si
    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and <t>BL21</t> Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).
    Bl21 Si, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SDS-PAGE (12.5% polyacrylamide gel) of rATB. Lanes: 1: molecular size markers; 2: E. coli BL21 (pET28arATB) prior to induction; 3: E. coli BL21 (pET28arATB) after induction; 4: 5 μ g denatured rATB in inclusion bodies; 5: 1 μ g refolded soluble rATB.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Production of Active Nonglycosylated Recombinant B-Chain of Type-2 Ribosome-Inactivating Protein from Viscum articulatum and Its Biological Effects on Peripheral Blood Mononuclear Cells

    doi: 10.1155/2011/283747

    Figure Lengend Snippet: SDS-PAGE (12.5% polyacrylamide gel) of rATB. Lanes: 1: molecular size markers; 2: E. coli BL21 (pET28arATB) prior to induction; 3: E. coli BL21 (pET28arATB) after induction; 4: 5 μ g denatured rATB in inclusion bodies; 5: 1 μ g refolded soluble rATB.

    Article Snippet: Bacterial Strains and Plasmids Escherichia coli XL1 blue (F− φ 80(lacZ )ΔM15 ΔlacX74 hsdR (rk − , mk + ) ΔrecA1398 endA1 tonA ) and BL21 [F− ompT hsdSB (rB − mB − ) gal dcm (DE3) pLysS(CamR )] were obtained from Invitrogen.

    Techniques: SDS Page

    SDS-PAGE and western blot analysis of E. coli BL21-AI cells expressing B. melitensis Omp31, BP26, or 16. Panel A. SDS-PAGE analysis of E. coli BL21-AI cells harbouring pDESTOMPs of B. melitensis. (M) Marker lane, (1) uninduced, (2), (3) and (4) are Omp31, BP26, and Omp16, respectively. Panel B. Western blot analysis of induced samples using anti-HIS probe. Lanes (1), (2) and (3) are B. melitensis Omp31, BP26, and Omp16, respectively.

    Journal: Vaccine

    Article Title: Invasive Escherichia coli vaccines expressing Brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein BP26 confer protection in mice challenged with B. melitensis

    doi: 10.1016/j.vaccine.2012.04.036

    Figure Lengend Snippet: SDS-PAGE and western blot analysis of E. coli BL21-AI cells expressing B. melitensis Omp31, BP26, or 16. Panel A. SDS-PAGE analysis of E. coli BL21-AI cells harbouring pDESTOMPs of B. melitensis. (M) Marker lane, (1) uninduced, (2), (3) and (4) are Omp31, BP26, and Omp16, respectively. Panel B. Western blot analysis of induced samples using anti-HIS probe. Lanes (1), (2) and (3) are B. melitensis Omp31, BP26, and Omp16, respectively.

    Article Snippet: Construction of inv E. coli vaccines used co-transformation of BL21-AI cells (Invitrogen) with pGB2Ωinv-hly and pDEST17/Omps.

    Techniques: SDS Page, Western Blot, Expressing, Marker

    Physiological effects of rate of membrane protein production. CX21-GFP was produced by rapid, restrained, and basal expression in BL21-AI cells. A: Biomass production. Growth was monitored via OD 600 nm of culture. B: Cell viability analysis using the

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Physiological effects of rate of membrane protein production. CX21-GFP was produced by rapid, restrained, and basal expression in BL21-AI cells. A: Biomass production. Growth was monitored via OD 600 nm of culture. B: Cell viability analysis using the

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Produced, Expressing

    Validation of the restrained-expression method to produce toxic proteins. 6K protein, an ion channel from Sindibis virus, was expressed as a GFP fusion in BL21-AI and BL21(DE3) cells. Cell growth (A) was monitored at 0, 2, 6, and 20 h postinduction by

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Validation of the restrained-expression method to produce toxic proteins. 6K protein, an ion channel from Sindibis virus, was expressed as a GFP fusion in BL21-AI and BL21(DE3) cells. Cell growth (A) was monitored at 0, 2, 6, and 20 h postinduction by

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Expressing

    Comparative analysis of overexpression methods. A: Application of the restrained-expression method to improve expression of CX homologs. Fluorescence of GFP fused to CX homologs expressed in BL21-AI and BL21(DE3) cells induced with 0.01% (w/v) arabinose

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Comparative analysis of overexpression methods. A: Application of the restrained-expression method to improve expression of CX homologs. Fluorescence of GFP fused to CX homologs expressed in BL21-AI and BL21(DE3) cells induced with 0.01% (w/v) arabinose

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Over Expression, Expressing, Fluorescence

    Improving membrane protein expression. A and B: Tuning target gene expression in BL21-AI cells. Fluorescence (AFU/mL) of cultures expressing either GFP (A) or CX21-GFP (B) using restrained and rapid expression measured 20 h after addition of inducer(s)

    Journal: Protein Science : A Publication of the Protein Society

    Article Title: Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions

    doi: 10.1002/pro.535

    Figure Lengend Snippet: Improving membrane protein expression. A and B: Tuning target gene expression in BL21-AI cells. Fluorescence (AFU/mL) of cultures expressing either GFP (A) or CX21-GFP (B) using restrained and rapid expression measured 20 h after addition of inducer(s)

    Article Snippet: The following E. coli strains were used to express proteins from T7 lac promoter based vectors: BL21(DE3) and BL21(DE3) pLysS (Novagen, CA), C43(DE3) (Lucigen, WI), Lemo21(DE3) (NEB, MA), and BL21-AI (Invitrogen, CA).

    Techniques: Expressing, Fluorescence

    Expression and purification of fusion protein. 1—pET16b–scFv-DAF/BL21 (DE3) plyss before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.

    Journal: Muscle & nerve

    Article Title: PROTECTIVE EFFECT OF ScFv-DAF FUSION PROTEIN ON THE COMPLEMENT ATTACK TO ACETYLCHOLINE RECEPTOR: A POSSIBLE OPTION FOR TREATMENT OF MYASTHENIA GRAVIS

    doi: 10.1002/mus.23247

    Figure Lengend Snippet: Expression and purification of fusion protein. 1—pET16b–scFv-DAF/BL21 (DE3) plyss before induction; 2—pET16b–scFv-DAF induced by 1 mM IPTG for 4 h; 3 and 4—eluted peak of fusion protein from Hitrap chelating HP column; 5 and 6—pET16b–scFv-DAF refolded with the optimized urea gradient dialysis method. M, low molecular protein markers. The black arrow on lane 2 indicates the additional band at 61 kDa.

    Article Snippet: BL21 (DE3) pLyss (Invitrogen) bacteria transformed with the reconstructed plasmid pET16b were grown at 37°C in 2× YT medium containing 100 μ g/ml ampicillin and 50 μ g/ml chloramphenicol.

    Techniques: Expressing, Purification

    Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and BL21 Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: Cell density at induction and harvesting. Expression tests of archaeal transporters were performed in E. coli C43(DE3) and BL21 Star strains. Cells were grown in LB medium, induced with 0.5 mM IPTG at OD 600nm of 0.4-0.8 and harvested 3 hours post-induction. The bars show the OD 600nm at the time of induction (white) and harvesting (black).

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: Expressing

    Affinity purification of seven membrane transport proteins. ( A ) Protein purification by Ni-NTA chromatography of 13 constructs expressed in E. coli BL21 Star. SDS-PAGE (top panel) and Western blot (bottom panel) of purified protein. MW - Bio-rad precision plus protein standards. ( B ) Estimated protein yield per litre of cell culture after initial Ni-NTA affinity chromatography. N/D - not determined.

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: Affinity purification of seven membrane transport proteins. ( A ) Protein purification by Ni-NTA chromatography of 13 constructs expressed in E. coli BL21 Star. SDS-PAGE (top panel) and Western blot (bottom panel) of purified protein. MW - Bio-rad precision plus protein standards. ( B ) Estimated protein yield per litre of cell culture after initial Ni-NTA affinity chromatography. N/D - not determined.

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: Affinity Purification, Protein Purification, Chromatography, Construct, SDS Page, Western Blot, Purification, Cell Culture, Affinity Chromatography

    Western blot analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed Western blotting. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-).

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: Western blot analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed Western blotting. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-).

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: Western Blot, Clone Assay

    SDS-PAGE analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed by SDS-PAGE. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-). Positions of the His-tagged protein determined by Western blotting (see Figure 3 ) are indicated by: triangle - protein detected by Western blotting but not visible on SDS-PAGE; star - protein detected by Western blotting and visible on SDS-PAGE gels.

    Journal: PLoS ONE

    Article Title: An Efficient Strategy for Small-Scale Screening and Production of Archaeal Membrane Transport Proteins in Escherichia coli

    doi: 10.1371/journal.pone.0076913

    Figure Lengend Snippet: SDS-PAGE analysis of archaeal membrane transport proteins in E. coli membranes. Membrane transport protein genes cloned into pTTQ18, pET52b and pWarf were tested in two E. coli host strains, C43(DE3) and BL21 Star. Membranes were analysed by SDS-PAGE. C - C43(DE3), S - BL21 Star, 1 - pTTQ18, 2 - pET52b(+), 3 - pWarf(-). Positions of the His-tagged protein determined by Western blotting (see Figure 3 ) are indicated by: triangle - protein detected by Western blotting but not visible on SDS-PAGE; star - protein detected by Western blotting and visible on SDS-PAGE gels.

    Article Snippet: Over-production of archaeal membrane transport proteins Expression constructs were used to transform two E. coli strains, BL21 Star (Invitrogen) and C43(DE3) [ ], containing the pRARE2 plasmid.

    Techniques: SDS Page, Clone Assay, Western Blot