bisulfite-specific pcr Search Results


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  • 97
    Zymo Research bisulfite converted
    Analysis of NDRG2 gene promoter methylation in PA samples. a Methylation frequency (%). b Hormone distribution of PAs in methylated and unmethylated NDRG2 promoter groups. PRL – prolactin, IGF-1 – insulin-like grow factor 1, GH – growth hormone, ACTH – adrenocorticotropic hormone, multiple – PAs secreting more than one hormone, NS – non-secreting PAs. c Representative <t>MS-PCR</t> for NDRG2 in PA samples. M indicates amplification of methylated alleles, U unmethylated alleles. M cont. – positive methylation control (Standard Bisulfite Converted Universal Methylated Human <t>DNA),</t> U cont. – negative methylation control (normal human peripheral lymphocytes), H2O – water control, I-VI designate PA samples
    Bisulfite Converted, supplied by Zymo Research, used in various techniques. Bioz Stars score: 97/100, based on 2312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisulfite converted/product/Zymo Research
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    97
    Thermo Fisher methylation specific pcr msp methyl primer express v1 0 software
    Analysis of NDRG2 gene promoter methylation in PA samples. a Methylation frequency (%). b Hormone distribution of PAs in methylated and unmethylated NDRG2 promoter groups. PRL – prolactin, IGF-1 – insulin-like grow factor 1, GH – growth hormone, ACTH – adrenocorticotropic hormone, multiple – PAs secreting more than one hormone, NS – non-secreting PAs. c Representative <t>MS-PCR</t> for NDRG2 in PA samples. M indicates amplification of methylated alleles, U unmethylated alleles. M cont. – positive methylation control (Standard Bisulfite Converted Universal Methylated Human <t>DNA),</t> U cont. – negative methylation control (normal human peripheral lymphocytes), H2O – water control, I-VI designate PA samples
    Methylation Specific Pcr Msp Methyl Primer Express V1 0 Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methylation specific pcr msp methyl primer express v1 0 software/product/Thermo Fisher
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    85
    Active Motif methylation specific polymerase chain reaction msp bisulfite conversion
    Bisulfite sequencing of the IGSF4 promoter region . The IGSF4 promoter region (559 bp, 52 CpG sites) was sequenced after bisulfite conversion of <t>DNA</t> in two cell lines and selected AML patients. CpGs are represented as open dots (unmethylated) or filled dots (methylated). In MLL wt AML patients and in the MLL wt cell line U-937 clones with CpG methylation next to the transcriptional start site (TSS) were detected whereas no methylation was detectable in the MLL mu patient analyzed and in the MLL mu cell line THP-1. Note that CpG sites analyzed by the MS-MLPA probe (green) and <t>MSP</t> primers (red) are not identical.
    Methylation Specific Polymerase Chain Reaction Msp Bisulfite Conversion, supplied by Active Motif, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore methylation specific pcr sodium bisulfite modification
    Bisulfite sequencing of the IGSF4 promoter region . The IGSF4 promoter region (559 bp, 52 CpG sites) was sequenced after bisulfite conversion of <t>DNA</t> in two cell lines and selected AML patients. CpGs are represented as open dots (unmethylated) or filled dots (methylated). In MLL wt AML patients and in the MLL wt cell line U-937 clones with CpG methylation next to the transcriptional start site (TSS) were detected whereas no methylation was detectable in the MLL mu patient analyzed and in the MLL mu cell line THP-1. Note that CpG sites analyzed by the MS-MLPA probe (green) and <t>MSP</t> primers (red) are not identical.
    Methylation Specific Pcr Sodium Bisulfite Modification, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Eurofins bisulfite specific primers
    Bisulfite sequencing of the IGSF4 promoter region . The IGSF4 promoter region (559 bp, 52 CpG sites) was sequenced after bisulfite conversion of <t>DNA</t> in two cell lines and selected AML patients. CpGs are represented as open dots (unmethylated) or filled dots (methylated). In MLL wt AML patients and in the MLL wt cell line U-937 clones with CpG methylation next to the transcriptional start site (TSS) were detected whereas no methylation was detectable in the MLL mu patient analyzed and in the MLL mu cell line THP-1. Note that CpG sites analyzed by the MS-MLPA probe (green) and <t>MSP</t> primers (red) are not identical.
    Bisulfite Specific Primers, supplied by Eurofins, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen epitect fast dna bisulfite kit
    5-methylcytosine detection using mass spectrometry and bisulfite sequencing. (a) <t>DNA</t> mass spectrometry analysis showing the percentage of cytosine methylation, vegetative unstarved; wild type cells were allowed to undergo vegetative divisions, starved vegetative; wild type vegetative cells grown four divisions and then starved for 24hours before extracting gDNA, 40% fragmented; a population where about 40% of cells have fragmented parental macronucleus during autogamy, post-autogamous; cells after seven days post autogamy. (b) Bisulfite sequencing analysis on different developmental stages; veg represents wild type vegetative cells, Veg starved is the stage where wild type cells were allowed to make four divisions and then was starved for 24hrs to block further vegetative divisions mac dev represents 40% fragmented, (c) Lambda DNA was spiked in sequencing samples as a negative control. (d) raw data from a published human bisulfite dataset was downloaded and ran through the workflow. (e) snapshot of mapping of putative loci for methylated cytosine (marked with orange bar) with the reference genome. Genomic DNA was treated with <t>Epitect</t> Fast DNA Bisulfite Kit (Qiagen) for Bisulfite conversion, PCR product was then sent for Sanger sequencing and mapped with Geneious software version R8.
    Epitect Fast Dna Bisulfite Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche bisulfite treated dna
    5-methylcytosine detection using mass spectrometry and bisulfite sequencing. (a) <t>DNA</t> mass spectrometry analysis showing the percentage of cytosine methylation, vegetative unstarved; wild type cells were allowed to undergo vegetative divisions, starved vegetative; wild type vegetative cells grown four divisions and then starved for 24hours before extracting gDNA, 40% fragmented; a population where about 40% of cells have fragmented parental macronucleus during autogamy, post-autogamous; cells after seven days post autogamy. (b) Bisulfite sequencing analysis on different developmental stages; veg represents wild type vegetative cells, Veg starved is the stage where wild type cells were allowed to make four divisions and then was starved for 24hrs to block further vegetative divisions mac dev represents 40% fragmented, (c) Lambda DNA was spiked in sequencing samples as a negative control. (d) raw data from a published human bisulfite dataset was downloaded and ran through the workflow. (e) snapshot of mapping of putative loci for methylated cytosine (marked with orange bar) with the reference genome. Genomic DNA was treated with <t>Epitect</t> Fast DNA Bisulfite Kit (Qiagen) for Bisulfite conversion, PCR product was then sent for Sanger sequencing and mapped with Geneious software version R8.
    Bisulfite Treated Dna, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    EpiGentek bisulflash bisulfite conversion kit
    5-methylcytosine detection using mass spectrometry and bisulfite sequencing. (a) <t>DNA</t> mass spectrometry analysis showing the percentage of cytosine methylation, vegetative unstarved; wild type cells were allowed to undergo vegetative divisions, starved vegetative; wild type vegetative cells grown four divisions and then starved for 24hours before extracting gDNA, 40% fragmented; a population where about 40% of cells have fragmented parental macronucleus during autogamy, post-autogamous; cells after seven days post autogamy. (b) Bisulfite sequencing analysis on different developmental stages; veg represents wild type vegetative cells, Veg starved is the stage where wild type cells were allowed to make four divisions and then was starved for 24hrs to block further vegetative divisions mac dev represents 40% fragmented, (c) Lambda DNA was spiked in sequencing samples as a negative control. (d) raw data from a published human bisulfite dataset was downloaded and ran through the workflow. (e) snapshot of mapping of putative loci for methylated cytosine (marked with orange bar) with the reference genome. Genomic DNA was treated with <t>Epitect</t> Fast DNA Bisulfite Kit (Qiagen) for Bisulfite conversion, PCR product was then sent for Sanger sequencing and mapped with Geneious software version R8.
    Bisulflash Bisulfite Conversion Kit, supplied by EpiGentek, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bisulflash bisulfite conversion kit/product/EpiGentek
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    95
    Zymo Research sodium bisulfite methylated human dna standard
    5-methylcytosine detection using mass spectrometry and bisulfite sequencing. (a) <t>DNA</t> mass spectrometry analysis showing the percentage of cytosine methylation, vegetative unstarved; wild type cells were allowed to undergo vegetative divisions, starved vegetative; wild type vegetative cells grown four divisions and then starved for 24hours before extracting gDNA, 40% fragmented; a population where about 40% of cells have fragmented parental macronucleus during autogamy, post-autogamous; cells after seven days post autogamy. (b) Bisulfite sequencing analysis on different developmental stages; veg represents wild type vegetative cells, Veg starved is the stage where wild type cells were allowed to make four divisions and then was starved for 24hrs to block further vegetative divisions mac dev represents 40% fragmented, (c) Lambda DNA was spiked in sequencing samples as a negative control. (d) raw data from a published human bisulfite dataset was downloaded and ran through the workflow. (e) snapshot of mapping of putative loci for methylated cytosine (marked with orange bar) with the reference genome. Genomic DNA was treated with <t>Epitect</t> Fast DNA Bisulfite Kit (Qiagen) for Bisulfite conversion, PCR product was then sent for Sanger sequencing and mapped with Geneious software version R8.
    Sodium Bisulfite Methylated Human Dna Standard, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Zymo Research methylation specific pcr ez dna methylation gold kit
    MiR-1247 is deregulated by methylation in non-small lung cancer tissues and cell lines. Notes: ( A ) The <t>DNA</t> methylation level of miR-1247 in 21 pairs of NSCLC and adjacent tissue samples were analyzed by MSP. ( B ) MiR-1247 expression levels were detected by <t>qRT-PCR</t> in 21 NSCLC and 21 normal tissue samples. ( C ) MSP analysis of the DNA methylation level of miR-1247 in HBE, A549, H460, and H1299 cell lines. ( D ) MiR-1247 levels of HBE, A549, H1299, H460 cells, performed by qRT-PCR. ( E ) STMN1 levels. ( F ) The STMN1 protein expression was analyzed by Western blot. Bars represent mean values ± standard deviation. *** P
    Methylation Specific Pcr Ez Dna Methylation Gold Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methylation specific pcr ez dna methylation gold kit/product/Zymo Research
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    Image Search Results


    Analysis of NDRG2 gene promoter methylation in PA samples. a Methylation frequency (%). b Hormone distribution of PAs in methylated and unmethylated NDRG2 promoter groups. PRL – prolactin, IGF-1 – insulin-like grow factor 1, GH – growth hormone, ACTH – adrenocorticotropic hormone, multiple – PAs secreting more than one hormone, NS – non-secreting PAs. c Representative MS-PCR for NDRG2 in PA samples. M indicates amplification of methylated alleles, U unmethylated alleles. M cont. – positive methylation control (Standard Bisulfite Converted Universal Methylated Human DNA), U cont. – negative methylation control (normal human peripheral lymphocytes), H2O – water control, I-VI designate PA samples

    Journal: Diagnostic Pathology

    Article Title: N-myc downstream-regulated gene 2 (NDRG2) promoter methylation and expression in pituitary adenoma

    doi: 10.1186/s13000-017-0622-7

    Figure Lengend Snippet: Analysis of NDRG2 gene promoter methylation in PA samples. a Methylation frequency (%). b Hormone distribution of PAs in methylated and unmethylated NDRG2 promoter groups. PRL – prolactin, IGF-1 – insulin-like grow factor 1, GH – growth hormone, ACTH – adrenocorticotropic hormone, multiple – PAs secreting more than one hormone, NS – non-secreting PAs. c Representative MS-PCR for NDRG2 in PA samples. M indicates amplification of methylated alleles, U unmethylated alleles. M cont. – positive methylation control (Standard Bisulfite Converted Universal Methylated Human DNA), U cont. – negative methylation control (normal human peripheral lymphocytes), H2O – water control, I-VI designate PA samples

    Article Snippet: For each set of methylation-specific PCR reactions methylated (Bisulfite-Converted Universal Methylated Human DNA Standard (Zymo Research, USA)), unmethylated (human blood lymphocyte DNA, treated with bisulfite) and negative (nuclease-free water) controls were included in all reactions.

    Techniques: Methylation, Mass Spectrometry, Polymerase Chain Reaction, Amplification

    Comparison of DNA copy numbers obtained using different techniques. DNA copy numbers of methylated/unmethylated DNA standards based on specifications of manufacturer (Expected), measured by flourometer and of p14 by Restriction Enzyme (RE) dPCR and p14 and COL2A1 with MethyLight dPCR in the 0% and 100% methylated samples. Copy numbers shown were obtained from 5 ng starting material (based on expected DNA quantity), pre-bisulfite conversion and RE digestion. RE dPCR data shows p14 copy number from the mock, MSRE and MDRE treatments. MethyLight dPCR data shows copy number obtained using the p14_M assay in singleplex and the methylation independent control COL2A1. Statistical comparisons are for Student’s t-test (* = p

    Journal: BMC Genomics

    Article Title: Quantification of epigenetic biomarkers: an evaluation of established and emerging methods for DNA methylation analysis

    doi: 10.1186/1471-2164-15-1174

    Figure Lengend Snippet: Comparison of DNA copy numbers obtained using different techniques. DNA copy numbers of methylated/unmethylated DNA standards based on specifications of manufacturer (Expected), measured by flourometer and of p14 by Restriction Enzyme (RE) dPCR and p14 and COL2A1 with MethyLight dPCR in the 0% and 100% methylated samples. Copy numbers shown were obtained from 5 ng starting material (based on expected DNA quantity), pre-bisulfite conversion and RE digestion. RE dPCR data shows p14 copy number from the mock, MSRE and MDRE treatments. MethyLight dPCR data shows copy number obtained using the p14_M assay in singleplex and the methylation independent control COL2A1. Statistical comparisons are for Student’s t-test (* = p

    Article Snippet: For MethyLight qPCR, quantification was performed using the standard curve method with Bisulfite Converted Methylated Human DNA from Zymo Research.

    Techniques: Methylation, Digital PCR

    Relationship between the starting amount of genomic DNA used for bisulfite conversion and percent methylation . Column-purified BSC DNA was diluted to 1 ng/μL and used as a DNA template for HRM analysis for LINE-1 methylation. Values are expressed as mean percent ± S.D.

    Journal: BMC Research Notes

    Article Title: A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation

    doi: 10.1186/1756-0500-4-565

    Figure Lengend Snippet: Relationship between the starting amount of genomic DNA used for bisulfite conversion and percent methylation . Column-purified BSC DNA was diluted to 1 ng/μL and used as a DNA template for HRM analysis for LINE-1 methylation. Values are expressed as mean percent ± S.D.

    Article Snippet: To test the reliability of using the NanoDrop to quantify the amount of DNA after bisulfite conversion, further experiments were conducted to compare this spectral absorbance method with qPCR using a commercially available standard BSC DNA (D5015, Zymo Research, Irvine CA, USA) supplied at a concentration of 20 ng/μL to produce a qPCR standard curve.

    Techniques: Methylation, Purification

    Raw Melt curves (in triplicates) for 100% methylated (red) and 100% unmethylated (blue) BSC DNA standards . Melt curve peaks differed by approximately 2°C and had characteristically different shapes indicating that the methylated and unmethylated DNA are different products.

    Journal: BMC Research Notes

    Article Title: A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation

    doi: 10.1186/1756-0500-4-565

    Figure Lengend Snippet: Raw Melt curves (in triplicates) for 100% methylated (red) and 100% unmethylated (blue) BSC DNA standards . Melt curve peaks differed by approximately 2°C and had characteristically different shapes indicating that the methylated and unmethylated DNA are different products.

    Article Snippet: To test the reliability of using the NanoDrop to quantify the amount of DNA after bisulfite conversion, further experiments were conducted to compare this spectral absorbance method with qPCR using a commercially available standard BSC DNA (D5015, Zymo Research, Irvine CA, USA) supplied at a concentration of 20 ng/μL to produce a qPCR standard curve.

    Techniques: Methylation

    Relationship between the amount of BSC DNA template from blood and LINE-1 methylation . The amount of BSC DNA was varied (left to right, 10 (red), 5 (blue), 2.0 (green), 1 (purple), 0.5 (light blue) and 0.1 ng (light green)) and HRM was used to measure LINE-1 methylation. (A) Fluorescence curves for varying amounts of DNA. Each sample was analyzed in triplicate. Note that at 40 cycles, the 0.1 ng curve did not fully reach a plateau. (B) Determination of percent methylation as a function of amount of template BSC DNA. Values are expressed as mean percent ± S.D. Data were analyzed by one-way ANOVA with Tukey's post hoc test.

    Journal: BMC Research Notes

    Article Title: A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation

    doi: 10.1186/1756-0500-4-565

    Figure Lengend Snippet: Relationship between the amount of BSC DNA template from blood and LINE-1 methylation . The amount of BSC DNA was varied (left to right, 10 (red), 5 (blue), 2.0 (green), 1 (purple), 0.5 (light blue) and 0.1 ng (light green)) and HRM was used to measure LINE-1 methylation. (A) Fluorescence curves for varying amounts of DNA. Each sample was analyzed in triplicate. Note that at 40 cycles, the 0.1 ng curve did not fully reach a plateau. (B) Determination of percent methylation as a function of amount of template BSC DNA. Values are expressed as mean percent ± S.D. Data were analyzed by one-way ANOVA with Tukey's post hoc test.

    Article Snippet: To test the reliability of using the NanoDrop to quantify the amount of DNA after bisulfite conversion, further experiments were conducted to compare this spectral absorbance method with qPCR using a commercially available standard BSC DNA (D5015, Zymo Research, Irvine CA, USA) supplied at a concentration of 20 ng/μL to produce a qPCR standard curve.

    Techniques: Methylation, Fluorescence

    Fluorescence curves (in triplicate) of fully methylated (red) and fully unmethylated (blue) BSC DNA standards . It is important that both methylated and unmethylated DNA are amplified with similar efficiencies, thereby indicating no PCR bias for either template DNA.

    Journal: BMC Research Notes

    Article Title: A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation

    doi: 10.1186/1756-0500-4-565

    Figure Lengend Snippet: Fluorescence curves (in triplicate) of fully methylated (red) and fully unmethylated (blue) BSC DNA standards . It is important that both methylated and unmethylated DNA are amplified with similar efficiencies, thereby indicating no PCR bias for either template DNA.

    Article Snippet: To test the reliability of using the NanoDrop to quantify the amount of DNA after bisulfite conversion, further experiments were conducted to compare this spectral absorbance method with qPCR using a commercially available standard BSC DNA (D5015, Zymo Research, Irvine CA, USA) supplied at a concentration of 20 ng/μL to produce a qPCR standard curve.

    Techniques: Fluorescence, Methylation, Amplification, Polymerase Chain Reaction

    Bisulfite sequencing of the IGSF4 promoter region . The IGSF4 promoter region (559 bp, 52 CpG sites) was sequenced after bisulfite conversion of DNA in two cell lines and selected AML patients. CpGs are represented as open dots (unmethylated) or filled dots (methylated). In MLL wt AML patients and in the MLL wt cell line U-937 clones with CpG methylation next to the transcriptional start site (TSS) were detected whereas no methylation was detectable in the MLL mu patient analyzed and in the MLL mu cell line THP-1. Note that CpG sites analyzed by the MS-MLPA probe (green) and MSP primers (red) are not identical.

    Journal: Molecular Cancer

    Article Title: Hypomethylation and expression of BEX2, IGSF4 and TIMP3 indicative of MLL translocations in Acute Myeloid Leukemia

    doi: 10.1186/1476-4598-8-86

    Figure Lengend Snippet: Bisulfite sequencing of the IGSF4 promoter region . The IGSF4 promoter region (559 bp, 52 CpG sites) was sequenced after bisulfite conversion of DNA in two cell lines and selected AML patients. CpGs are represented as open dots (unmethylated) or filled dots (methylated). In MLL wt AML patients and in the MLL wt cell line U-937 clones with CpG methylation next to the transcriptional start site (TSS) were detected whereas no methylation was detectable in the MLL mu patient analyzed and in the MLL mu cell line THP-1. Note that CpG sites analyzed by the MS-MLPA probe (green) and MSP primers (red) are not identical.

    Article Snippet: Methylation-specific polymerase chain reaction (MSP) Bisulfite conversion of DNA was performed as described by the supplier (Active Motif, Rixensart, Belgium).

    Techniques: Methylation Sequencing, Methylation, Clone Assay, CpG Methylation Assay, Mass Spectrometry, Multiplex Ligation-dependent Probe Amplification

    5-methylcytosine detection using mass spectrometry and bisulfite sequencing. (a) DNA mass spectrometry analysis showing the percentage of cytosine methylation, vegetative unstarved; wild type cells were allowed to undergo vegetative divisions, starved vegetative; wild type vegetative cells grown four divisions and then starved for 24hours before extracting gDNA, 40% fragmented; a population where about 40% of cells have fragmented parental macronucleus during autogamy, post-autogamous; cells after seven days post autogamy. (b) Bisulfite sequencing analysis on different developmental stages; veg represents wild type vegetative cells, Veg starved is the stage where wild type cells were allowed to make four divisions and then was starved for 24hrs to block further vegetative divisions mac dev represents 40% fragmented, (c) Lambda DNA was spiked in sequencing samples as a negative control. (d) raw data from a published human bisulfite dataset was downloaded and ran through the workflow. (e) snapshot of mapping of putative loci for methylated cytosine (marked with orange bar) with the reference genome. Genomic DNA was treated with Epitect Fast DNA Bisulfite Kit (Qiagen) for Bisulfite conversion, PCR product was then sent for Sanger sequencing and mapped with Geneious software version R8.

    Journal: PLoS ONE

    Article Title: Determination of the presence of 5-methylcytosine in Paramecium tetraurelia

    doi: 10.1371/journal.pone.0206667

    Figure Lengend Snippet: 5-methylcytosine detection using mass spectrometry and bisulfite sequencing. (a) DNA mass spectrometry analysis showing the percentage of cytosine methylation, vegetative unstarved; wild type cells were allowed to undergo vegetative divisions, starved vegetative; wild type vegetative cells grown four divisions and then starved for 24hours before extracting gDNA, 40% fragmented; a population where about 40% of cells have fragmented parental macronucleus during autogamy, post-autogamous; cells after seven days post autogamy. (b) Bisulfite sequencing analysis on different developmental stages; veg represents wild type vegetative cells, Veg starved is the stage where wild type cells were allowed to make four divisions and then was starved for 24hrs to block further vegetative divisions mac dev represents 40% fragmented, (c) Lambda DNA was spiked in sequencing samples as a negative control. (d) raw data from a published human bisulfite dataset was downloaded and ran through the workflow. (e) snapshot of mapping of putative loci for methylated cytosine (marked with orange bar) with the reference genome. Genomic DNA was treated with Epitect Fast DNA Bisulfite Kit (Qiagen) for Bisulfite conversion, PCR product was then sent for Sanger sequencing and mapped with Geneious software version R8.

    Article Snippet: There was no bias in C conversion among the three different nucleotide contexts ( , right panel) Furthermore, we tested specific loci using the Epitect Fast DNA Bisulfite Kit (Qiagen) followed by Sanger sequencing.

    Techniques: Mass Spectrometry, Methylation Sequencing, Methylation, Blocking Assay, Lambda DNA Preparation, Sequencing, Negative Control, Polymerase Chain Reaction, Software

    MiR-1247 is deregulated by methylation in non-small lung cancer tissues and cell lines. Notes: ( A ) The DNA methylation level of miR-1247 in 21 pairs of NSCLC and adjacent tissue samples were analyzed by MSP. ( B ) MiR-1247 expression levels were detected by qRT-PCR in 21 NSCLC and 21 normal tissue samples. ( C ) MSP analysis of the DNA methylation level of miR-1247 in HBE, A549, H460, and H1299 cell lines. ( D ) MiR-1247 levels of HBE, A549, H1299, H460 cells, performed by qRT-PCR. ( E ) STMN1 levels. ( F ) The STMN1 protein expression was analyzed by Western blot. Bars represent mean values ± standard deviation. *** P

    Journal: OncoTargets and therapy

    Article Title: Silencing of miR-1247 by DNA methylation promoted non-small-cell lung cancer cell invasion and migration by effects of STMN1

    doi: 10.2147/OTT.S111291

    Figure Lengend Snippet: MiR-1247 is deregulated by methylation in non-small lung cancer tissues and cell lines. Notes: ( A ) The DNA methylation level of miR-1247 in 21 pairs of NSCLC and adjacent tissue samples were analyzed by MSP. ( B ) MiR-1247 expression levels were detected by qRT-PCR in 21 NSCLC and 21 normal tissue samples. ( C ) MSP analysis of the DNA methylation level of miR-1247 in HBE, A549, H460, and H1299 cell lines. ( D ) MiR-1247 levels of HBE, A549, H1299, H460 cells, performed by qRT-PCR. ( E ) STMN1 levels. ( F ) The STMN1 protein expression was analyzed by Western blot. Bars represent mean values ± standard deviation. *** P

    Article Snippet: Methylation-specific PCR EZ DNA Methylation-Gold™ Kit (Zymo Research, Irvine, CA, USA) was used to modify genomic DNA, and MSP was used to detect the methylation level of miR-1247 in cells and tissues.

    Techniques: Methylation, DNA Methylation Assay, Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation