bis-tris propane Search Results


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  • 99
    New England Biolabs ne buffer 1
    Ne Buffer 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ne buffer 1/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
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    ne buffer 1 - by Bioz Stars, 2020-03
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    99
    Thermo Fisher tris solution
    Tris Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 19 article reviews
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    99
    Millipore bistris propane
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bistris Propane, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore btp 1 3 bis tris hydroxymethyl methylamino propane
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Btp 1 3 Bis Tris Hydroxymethyl Methylamino Propane, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Jena Bioscience bis tris propane hcl
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bis Tris Propane Hcl, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 98/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    ATUM bis tris propane
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bis Tris Propane, supplied by ATUM, used in various techniques. Bioz Stars score: 81/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Nacalai bis tris propane
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bis Tris Propane, supplied by Nacalai, used in various techniques. Bioz Stars score: 81/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    GE Healthcare bis tris propane btp
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bis Tris Propane Btp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 87/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore bis tris propane 2 2 propane 1 3 diyldiimino bis 2 hydroxymethyl propane 1
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bis Tris Propane 2 2 Propane 1 3 Diyldiimino Bis 2 Hydroxymethyl Propane 1, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Thermo Fisher bis tris 2 bis 2 hydroxyethyl amino 2 hydroxymethyl propane 1
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bis Tris 2 Bis 2 Hydroxyethyl Amino 2 Hydroxymethyl Propane 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Bio-Rad g1 dpeaae bistris 2 bis 2 hydroxyethyl amino 2 hydroxymeth yl propane 1
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    G1 Dpeaae Bistris 2 Bis 2 Hydroxyethyl Amino 2 Hydroxymeth Yl Propane 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 3 article reviews
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    99
    Millipore bis tris
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Bis Tris, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore tris hydroxymethyl amino methane hydrochloride
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Tris Hydroxymethyl Amino Methane Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore buffer
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore source q resin
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Source Q Resin, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Millipore size exclusion chromatography buffer
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    Size Exclusion Chromatography Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    GE Healthcare 1 3 bis tris
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
    1 3 Bis Tris, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bruker Corporation nmr buffer
    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM <t>BIS-TRIS</t> propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P
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    Image Search Results


    Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM BIS-TRIS propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P

    Journal: Microbiology

    Article Title: Effects of chromosomal deletion of the operon encoding the multiple resistance and pH-related antiporter in Vibrio cholerae

    doi: 10.1099/mic.0.000384

    Figure Lengend Snippet: Loss of mrp causes increased swimming motility in soft agar. V. cholerae strains were grown overnight at 37 °C on LB agar. Single colonies were stabbed using a 10 µl pipette tip into (a) LBB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 60 mM BIS-TRIS propane, 0.2 % arabinose and 200 µg ml −1 ampicillin) and (b) LB− soft agar (10 g l −1 tryptone, 5 g l −1 yeast extract, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) at pH 8.5, 7.5, 6.5 and 5.5 and incubated for 24 h. Single colonies were stabbed using a 10 µl pipette tip into M9 minimal media soft agar (12.8 g l −1 Na 2 HPO 4 ·7H 2 O, 3 g l −1 KH 2 HPO 4 , 0.5 g l −1 NaCl, 1 g l −1 NH 4 Cl, 0.1 mM CaCl 2 , 2 mM MgSO 4 , 20 mM succinate, 0.3 % agar, 0.2 % arabinose and 200 µg ml −1 ampicillin) and incubated for (c) 24 or (d) 48 h. Soft agar plates were incubated at 37 °C for the indicated times and pictures were taken. Results presented are the mean±standard error of at least three independent experiments. Asterisks indicate statistical significance (* P

    Article Snippet: The LBB− plates were buffered with 60 mM BIS-TRIS propane (Sigma).

    Techniques: Transferring, Incubation