biotinylated secondary antibody Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Vector Laboratories biotinylated secondary antibodies
    Biotinylated Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 10949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Vector Laboratories
    Average 99 stars, based on 10949 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher biotinylated secondary antibodies
    Expression and nuclear translocation of IRF-3 after CSFV infection in porcine alveolar macrophages. (A) Expression of IRF-3 was measured by Western Blotting with antibodies specific for IRF-3, and the cells were treated as demonstrated in Figure 1 . (B) Indirect immunofluorescence analysis was used to measure cellular localization of IRF-3 in CSFV-infected PAMs. Cells were mock treated, poly (I:C) stimulated, or infected with Shimen isolates at an MOI of 3. At 24 hpi, cells were fixed and the localization of IRF-3 (red) was observed by fluorescence microscope using immunofluorescence stain with anti-IRF-3 and QDs-SA 605-conjugated and <t>biotinylated</t> secondary antibodies. Nuclei were stained with DAPI. Bar, 10 μm. The experiment was repeated three times and the figure shows a representative experiment. An asterisk indicates a statistically significant difference from uninfected cells, * P
    Biotinylated Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1348 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Thermo Fisher
    Average 99 stars, based on 1348 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Agilent technologies biotinylated secondary antibody
    Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, <t>biotinylated</t> ADSCs; Avi-β-TCP, avidin-coated β-TCP.
    Biotinylated Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 5121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Agilent technologies
    Average 94 stars, based on 5121 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated goat anti rabbit secondary antibody
    Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, <t>biotinylated</t> ADSCs; Avi-β-TCP, avidin-coated β-TCP.
    Biotinylated Goat Anti Rabbit Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 3869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 3869 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated anti rabbit secondary antibody
    Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, <t>biotinylated</t> ADSCs; Avi-β-TCP, avidin-coated β-TCP.
    Biotinylated Anti Rabbit Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1856 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    94
    Santa Cruz Biotechnology biotinylated secondary antibody
    B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary. Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with <t>biotinylated-DBA</t> lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.
    Biotinylated Secondary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1103 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated anti mouse secondary antibody
    B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary. Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with <t>biotinylated-DBA</t> lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.
    Biotinylated Anti Mouse Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti mouse secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1060 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti mouse secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated horse anti mouse secondary antibody
    B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary. Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with <t>biotinylated-DBA</t> lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.
    Biotinylated Horse Anti Mouse Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated horse anti mouse secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 1046 article reviews
    Price from $9.99 to $1999.99
    biotinylated horse anti mouse secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated goat anti rabbit igg secondary antibody
    B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary. Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with <t>biotinylated-DBA</t> lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.
    Biotinylated Goat Anti Rabbit Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 782 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti rabbit igg secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 782 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti rabbit igg secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated goat anti mouse secondary antibody
    B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary. Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with <t>biotinylated-DBA</t> lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.
    Biotinylated Goat Anti Mouse Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti mouse secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 689 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti mouse secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    92
    Abcam biotinylated secondary antibody
    Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) <t>avidin-biotin</t> method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).
    Biotinylated Secondary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Abcam
    Average 92 stars, based on 362 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    99
    Millipore biotinylated secondary ab
    Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) <t>avidin-biotin</t> method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).
    Biotinylated Secondary Ab, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary ab/product/Millipore
    Average 99 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary ab - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    93
    Zhongshan Golden Bridge Company biotinylated secondary antibody
    Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) <t>avidin-biotin</t> method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).
    Biotinylated Secondary Antibody, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 93/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Zhongshan Golden Bridge Company
    Average 93 stars, based on 424 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2020-10
    93/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated mouse secondary antibody
    Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) <t>avidin-biotin</t> method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).
    Biotinylated Mouse Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 480 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated mouse secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 480 article reviews
    Price from $9.99 to $1999.99
    biotinylated mouse secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    89
    Becton Dickinson biotinylated secondary ab
    The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. ( a ) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK ( a ), Raf-1 ( b ), p38-MAPK ( e ), and p46/p54–JNK1 ( f ) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. ( e ) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. ( c ) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. ( d ) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with <t>biotinylated</t> cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.
    Biotinylated Secondary Ab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary ab/product/Becton Dickinson
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary ab - by Bioz Stars, 2020-10
    89/100 stars
      Buy from Supplier

    93
    The Jackson Laboratory biotinylated secondary antibodies
    Effect of WNK2 and WNK3 on the protein level and surface expression of NKCC1 and KCC4. Western blot analysis of the total and <t>biotinylated</t> fraction of proteins extracted from oocytes injected with NKCC1 ( A ) or KCC4 ( B ) cRNA in the absence or presence of WNK2 or WNK3 cRNA. C and D depict the results of the functional expression performed the same day using oocytes from the same batch for NKCC1 or KCC4, respectively, expressed as bumetanide-sensitive ( C ) or chloride-dependent ( D ) 86 Rb + uptake.
    Biotinylated Secondary Antibodies, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 93/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/The Jackson Laboratory
    Average 93 stars, based on 244 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2020-10
    93/100 stars
      Buy from Supplier

    92
    Biogenex biotinylated secondary antibody
    Effect of WNK2 and WNK3 on the protein level and surface expression of NKCC1 and KCC4. Western blot analysis of the total and <t>biotinylated</t> fraction of proteins extracted from oocytes injected with NKCC1 ( A ) or KCC4 ( B ) cRNA in the absence or presence of WNK2 or WNK3 cRNA. C and D depict the results of the functional expression performed the same day using oocytes from the same batch for NKCC1 or KCC4, respectively, expressed as bumetanide-sensitive ( C ) or chloride-dependent ( D ) 86 Rb + uptake.
    Biotinylated Secondary Antibody, supplied by Biogenex, used in various techniques. Bioz Stars score: 92/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibody/product/Biogenex
    Average 92 stars, based on 237 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibody - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    92
    Dianova biotinylated secondary antibodies
    Effect of WNK2 and WNK3 on the protein level and surface expression of NKCC1 and KCC4. Western blot analysis of the total and <t>biotinylated</t> fraction of proteins extracted from oocytes injected with NKCC1 ( A ) or KCC4 ( B ) cRNA in the absence or presence of WNK2 or WNK3 cRNA. C and D depict the results of the functional expression performed the same day using oocytes from the same batch for NKCC1 or KCC4, respectively, expressed as bumetanide-sensitive ( C ) or chloride-dependent ( D ) 86 Rb + uptake.
    Biotinylated Secondary Antibodies, supplied by Dianova, used in various techniques. Bioz Stars score: 92/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary antibodies/product/Dianova
    Average 92 stars, based on 241 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary antibodies - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated universal secondary antibody
    Effect of WNK2 and WNK3 on the protein level and surface expression of NKCC1 and KCC4. Western blot analysis of the total and <t>biotinylated</t> fraction of proteins extracted from oocytes injected with NKCC1 ( A ) or KCC4 ( B ) cRNA in the absence or presence of WNK2 or WNK3 cRNA. C and D depict the results of the functional expression performed the same day using oocytes from the same batch for NKCC1 or KCC4, respectively, expressed as bumetanide-sensitive ( C ) or chloride-dependent ( D ) 86 Rb + uptake.
    Biotinylated Universal Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated universal secondary antibody/product/Vector Laboratories
    Average 99 stars, based on 477 article reviews
    Price from $9.99 to $1999.99
    biotinylated universal secondary antibody - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    92
    Agilent technologies biotinylated goat anti mouse secondary antibody
    Scheme of enzyme-enhanced LFIC for VCP detection <t>Biotinylated</t> gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.
    Biotinylated Goat Anti Mouse Secondary Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti mouse secondary antibody/product/Agilent technologies
    Average 92 stars, based on 352 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat anti mouse secondary antibody - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    Image Search Results


    Expression and nuclear translocation of IRF-3 after CSFV infection in porcine alveolar macrophages. (A) Expression of IRF-3 was measured by Western Blotting with antibodies specific for IRF-3, and the cells were treated as demonstrated in Figure 1 . (B) Indirect immunofluorescence analysis was used to measure cellular localization of IRF-3 in CSFV-infected PAMs. Cells were mock treated, poly (I:C) stimulated, or infected with Shimen isolates at an MOI of 3. At 24 hpi, cells were fixed and the localization of IRF-3 (red) was observed by fluorescence microscope using immunofluorescence stain with anti-IRF-3 and QDs-SA 605-conjugated and biotinylated secondary antibodies. Nuclei were stained with DAPI. Bar, 10 μm. The experiment was repeated three times and the figure shows a representative experiment. An asterisk indicates a statistically significant difference from uninfected cells, * P

    Journal: Virology Journal

    Article Title: Classical swine fever virus triggers RIG-I and MDA5-dependent signaling pathway to IRF-3 and NF-?B activation to promote secretion of interferon and inflammatory cytokines in porcine alveolar macrophages

    doi: 10.1186/1743-422X-10-286

    Figure Lengend Snippet: Expression and nuclear translocation of IRF-3 after CSFV infection in porcine alveolar macrophages. (A) Expression of IRF-3 was measured by Western Blotting with antibodies specific for IRF-3, and the cells were treated as demonstrated in Figure 1 . (B) Indirect immunofluorescence analysis was used to measure cellular localization of IRF-3 in CSFV-infected PAMs. Cells were mock treated, poly (I:C) stimulated, or infected with Shimen isolates at an MOI of 3. At 24 hpi, cells were fixed and the localization of IRF-3 (red) was observed by fluorescence microscope using immunofluorescence stain with anti-IRF-3 and QDs-SA 605-conjugated and biotinylated secondary antibodies. Nuclei were stained with DAPI. Bar, 10 μm. The experiment was repeated three times and the figure shows a representative experiment. An asterisk indicates a statistically significant difference from uninfected cells, * P

    Article Snippet: After washed three times with PBST, cells were further incubated with QDs-SA-conjugated and biotinylated secondary antibodies (1:100, Molecular Probes, Jiayuan Quantum Dots, Wuhai, China) at 37°C for 30 min.

    Techniques: Expressing, Translocation Assay, Infection, Western Blot, Immunofluorescence, Fluorescence, Microscopy, Staining

    Protein expression and nuclear translocation of NF- κB after CSFV infection in porcine alveolar macrophages. (A) Expression of NF-κB was measured by Western Blotting with antibodies specific for NF-κB, the cells were treated as demonstrated in Figure 1 . (B) Indirect immunofluorescence analysis was used to measure cellular localization of NF-κB in CSFV-infected PAMs. Cells were treated as indicated in Figure 3 . At 24 hpi, cells were fixed and the localization of NF-κB (red) was observed by fluorescence microscope using immunofluorescence stain with anti-NF-κB/p65 and QDs-SA 605-conjugated biotinylated secondary antibodies. Nuclei were stained with DAPI. Bar, 10 μm. Representative results are shown of one of three separate experiments. An asterisk indicates a statistically significant difference from uninfected cells, * P

    Journal: Virology Journal

    Article Title: Classical swine fever virus triggers RIG-I and MDA5-dependent signaling pathway to IRF-3 and NF-?B activation to promote secretion of interferon and inflammatory cytokines in porcine alveolar macrophages

    doi: 10.1186/1743-422X-10-286

    Figure Lengend Snippet: Protein expression and nuclear translocation of NF- κB after CSFV infection in porcine alveolar macrophages. (A) Expression of NF-κB was measured by Western Blotting with antibodies specific for NF-κB, the cells were treated as demonstrated in Figure 1 . (B) Indirect immunofluorescence analysis was used to measure cellular localization of NF-κB in CSFV-infected PAMs. Cells were treated as indicated in Figure 3 . At 24 hpi, cells were fixed and the localization of NF-κB (red) was observed by fluorescence microscope using immunofluorescence stain with anti-NF-κB/p65 and QDs-SA 605-conjugated biotinylated secondary antibodies. Nuclei were stained with DAPI. Bar, 10 μm. Representative results are shown of one of three separate experiments. An asterisk indicates a statistically significant difference from uninfected cells, * P

    Article Snippet: After washed three times with PBST, cells were further incubated with QDs-SA-conjugated and biotinylated secondary antibodies (1:100, Molecular Probes, Jiayuan Quantum Dots, Wuhai, China) at 37°C for 30 min.

    Techniques: Expressing, Translocation Assay, Infection, Western Blot, Immunofluorescence, Fluorescence, Microscopy, Staining

    Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated ADSCs; Avi-β-TCP, avidin-coated β-TCP.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

    doi: 10.3892/etm.2015.2961

    Figure Lengend Snippet: Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated ADSCs; Avi-β-TCP, avidin-coated β-TCP.

    Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

    Techniques: Electron Microscopy, Construct, Incubation, Derivative Assay, Avidin-Biotin Assay

    (A and B) Hematoxylin and eosin staining and (C and D) immunohistochemical analysis of osteocalcin protein expression at the mandibular defect area in the (A and C) group 1 and (B and D) group 2 rabbits at 4 weeks post-operation (magnification, ×200). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

    doi: 10.3892/etm.2015.2961

    Figure Lengend Snippet: (A and B) Hematoxylin and eosin staining and (C and D) immunohistochemical analysis of osteocalcin protein expression at the mandibular defect area in the (A and C) group 1 and (B and D) group 2 rabbits at 4 weeks post-operation (magnification, ×200). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

    Techniques: Staining, Immunohistochemistry, Expressing, Construct, Derivative Assay, Avidin-Biotin Assay

    Three-dimensional computed tomography images of mandibular defect reconstruction in the (A) group 1 and (B) group 2 rabbits at 4 weeks post-operation (red arrow). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

    doi: 10.3892/etm.2015.2961

    Figure Lengend Snippet: Three-dimensional computed tomography images of mandibular defect reconstruction in the (A) group 1 and (B) group 2 rabbits at 4 weeks post-operation (red arrow). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

    Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

    Techniques: Computed Tomography, Construct, Derivative Assay, Avidin-Biotin Assay

    B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary. Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with biotinylated-DBA lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.

    Journal: PLoS Genetics

    Article Title: The Highly Prolific Phenotype of Lacaune Sheep Is Associated with an Ectopic Expression of the B4GALNT2 Gene within the Ovary

    doi: 10.1371/journal.pgen.1003809

    Figure Lengend Snippet: B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary. Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with biotinylated-DBA lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 µM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.

    Article Snippet: After three 5-min washes in PBS containing 0.1% saponin, sections were incubated for 2 hours at room temperature with the biotinylated secondary antibody (donkey anti-goat, Santa Cruz Biotechnology; donkey anti-mouse, Jackson ImmunoResearch Laboratories) diluted 1∶800 in PBS containing 0.1% saponin and 0.1% BSA, and washed thereafter.

    Techniques: Activity Assay, Staining, Microscopy

    Western immunoblotting analysis of B4GALNT2 transferase activity in Lacaune sheep granulosa cells and follicular fluids. Granulosa cell protein extracts (50 µg) and follicular fluids (200 µg) from +/+ and L/L large antral follicles were precipitated (P) by agarose-DBA lectin or sepharose-protein A-KM694 monoclonal antibody. The resulting purified glycoproteins were separated on SDS-PAGE, transferred on nitrocellulose membrane and revealed after blotting (B) using biotinylated-DBA lectin or KM694 monoclonal antibody.

    Journal: PLoS Genetics

    Article Title: The Highly Prolific Phenotype of Lacaune Sheep Is Associated with an Ectopic Expression of the B4GALNT2 Gene within the Ovary

    doi: 10.1371/journal.pgen.1003809

    Figure Lengend Snippet: Western immunoblotting analysis of B4GALNT2 transferase activity in Lacaune sheep granulosa cells and follicular fluids. Granulosa cell protein extracts (50 µg) and follicular fluids (200 µg) from +/+ and L/L large antral follicles were precipitated (P) by agarose-DBA lectin or sepharose-protein A-KM694 monoclonal antibody. The resulting purified glycoproteins were separated on SDS-PAGE, transferred on nitrocellulose membrane and revealed after blotting (B) using biotinylated-DBA lectin or KM694 monoclonal antibody.

    Article Snippet: After three 5-min washes in PBS containing 0.1% saponin, sections were incubated for 2 hours at room temperature with the biotinylated secondary antibody (donkey anti-goat, Santa Cruz Biotechnology; donkey anti-mouse, Jackson ImmunoResearch Laboratories) diluted 1∶800 in PBS containing 0.1% saponin and 0.1% BSA, and washed thereafter.

    Techniques: Western Blot, Activity Assay, Purification, SDS Page

    B4GALNT2 transferase activity revealed by DBA lectin after in vitro overexpression of B4GALNT2 in ovine granulosa cells. Primary ovine granulosa cells from +/+ small antral follicles were transiently transfected with either the pCDNA-hB4GALNT2 expressing the human form of B4GALNT2 or the empty pCDNA3.1 vector. Twenty-four hours after transfection, cells were stained with biotinylated-DBA lectin (500 ng/ml). Arrows indicated DBA positive staining only in B4GALNT2 transfected cells. Cells were counterstained with hematoxylin. A black bar indicates the microscopy magnification scale.

    Journal: PLoS Genetics

    Article Title: The Highly Prolific Phenotype of Lacaune Sheep Is Associated with an Ectopic Expression of the B4GALNT2 Gene within the Ovary

    doi: 10.1371/journal.pgen.1003809

    Figure Lengend Snippet: B4GALNT2 transferase activity revealed by DBA lectin after in vitro overexpression of B4GALNT2 in ovine granulosa cells. Primary ovine granulosa cells from +/+ small antral follicles were transiently transfected with either the pCDNA-hB4GALNT2 expressing the human form of B4GALNT2 or the empty pCDNA3.1 vector. Twenty-four hours after transfection, cells were stained with biotinylated-DBA lectin (500 ng/ml). Arrows indicated DBA positive staining only in B4GALNT2 transfected cells. Cells were counterstained with hematoxylin. A black bar indicates the microscopy magnification scale.

    Article Snippet: After three 5-min washes in PBS containing 0.1% saponin, sections were incubated for 2 hours at room temperature with the biotinylated secondary antibody (donkey anti-goat, Santa Cruz Biotechnology; donkey anti-mouse, Jackson ImmunoResearch Laboratories) diluted 1∶800 in PBS containing 0.1% saponin and 0.1% BSA, and washed thereafter.

    Techniques: Activity Assay, In Vitro, Over Expression, Transfection, Expressing, Plasmid Preparation, Staining, Microscopy

    Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) avidin-biotin method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).

    Journal: Veterinary World

    Article Title: Detection of Toxoplasma gondii in cat’s internal organs by immunohistochemistry methods labeled with-[strept] avidin-biotin

    doi: 10.14202/vetworld.2017.1035-1039

    Figure Lengend Snippet: Toxoplasma positive immunohistochemistry staining of cat with labeled-(Strept) avidin-biotin method: (a) Necrosis is present in kidney and (b) ileum (arrow) (brown color is indicating the positive result of immunohistochemistry) (scale bar=30 µm).

    Article Snippet: Slides were incubated with primary polyclonal anti-T. gondii antibody (rabbit) primary antibody (Abcam, USA) diluted 1/100 at 37°C for 32 min and biotinylated secondary antibody (Abcam, USA).

    Techniques: Immunohistochemistry, Staining, Labeling, Avidin-Biotin Assay

    The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. ( a ) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK ( a ), Raf-1 ( b ), p38-MAPK ( e ), and p46/p54–JNK1 ( f ) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. ( e ) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. ( c ) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. ( d ) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with biotinylated cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.

    Journal: Journal of Clinical Investigation

    Article Title: Inhibition of endogenous thioredoxin in the heart increases oxidative stress and cardiac hypertrophy

    doi: 10.1172/JCI200317700

    Figure Lengend Snippet: The activities of the Ras–Raf-1–ERK pathway, p38-MAPK, and p46/p54–JNK1 were examined in Tg-DN-Trx1 mice and NTg littermates. ( a ) Some mice were subjected to aortic banding for 2 weeks. Activities of p42/p44–ERK ( a ), Raf-1 ( b ), p38-MAPK ( e ), and p46/p54–JNK1 ( f ) were determined using anti-phosphospecific Ab’s. The same filter was reprobed with respective non-phosphospecific Ab. ( e ) Cell extracts prepared from cardiac myocytes overexpressing mammalian sterile 20–like kinase 1 (Mst1) were used as positive control (P/C). The gel picture is representative of three to ten experiments in each immunoblot analysis. ( c ) The activity of Ras was determined using the Ras-binding domain in Raf-1 coupled with agarose. ( d ) S-thiolation of Ras was determined as described in Methods. COS-7 cells grown in 60-mm dishes were transfected with the indicated expression plasmids (2 μg). Cells were incubated with biotinylated cysteine (0.5 mM), and S-thiolated proteins were isolated by streptavidin-Sepharose. Samples were subjected to immunoblot analyses with anti-Ras Ab. AS-Trx1, antisense Trx1.

    Article Snippet: After washing, biotinylated secondary Ab (anti-mouse IgG; BD Pharmingen) was applied for 1 hour, followed by streptavidin-HRP (BD Pharmingen) for 30 minutes at room temperature.

    Techniques: Mouse Assay, Positive Control, Activity Assay, Binding Assay, Transfection, Expressing, Incubation, Isolation

    Effect of WNK2 and WNK3 on the protein level and surface expression of NKCC1 and KCC4. Western blot analysis of the total and biotinylated fraction of proteins extracted from oocytes injected with NKCC1 ( A ) or KCC4 ( B ) cRNA in the absence or presence of WNK2 or WNK3 cRNA. C and D depict the results of the functional expression performed the same day using oocytes from the same batch for NKCC1 or KCC4, respectively, expressed as bumetanide-sensitive ( C ) or chloride-dependent ( D ) 86 Rb + uptake.

    Journal: The Journal of Biological Chemistry

    Article Title: WNK2 Kinase Is a Novel Regulator of Essential Neuronal Cation-Chloride Cotransporters *

    doi: 10.1074/jbc.M111.222893

    Figure Lengend Snippet: Effect of WNK2 and WNK3 on the protein level and surface expression of NKCC1 and KCC4. Western blot analysis of the total and biotinylated fraction of proteins extracted from oocytes injected with NKCC1 ( A ) or KCC4 ( B ) cRNA in the absence or presence of WNK2 or WNK3 cRNA. C and D depict the results of the functional expression performed the same day using oocytes from the same batch for NKCC1 or KCC4, respectively, expressed as bumetanide-sensitive ( C ) or chloride-dependent ( D ) 86 Rb + uptake.

    Article Snippet: Following washes with PBS, they were incubated with biotinylated secondary antibodies (Jackson Laboratories, 1:250 dilution) for 2 h at room temperature, washed in PBS, and then incubated with Vectastain ABC Elite solution (Vector Laboratories, Burlingame, CA) for 2 h. Sections were developed using 0.05% DAB (pH 7.4), 0.2% glucose, 0.01% nickel ammonium sulfate, 0.04% ammonium chloride, and 8 mg/ml glucose oxidase, and then rinsed, mounted onto glass slides, allowed to dry, dehydrated, and coverslipped.

    Techniques: Expressing, Western Blot, Injection, Functional Assay

    Scheme of enzyme-enhanced LFIC for VCP detection Biotinylated gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.

    Journal: Oncotarget

    Article Title: Point-of-care test for cervical cancer in LMICs

    doi: 10.18632/oncotarget.7709

    Figure Lengend Snippet: Scheme of enzyme-enhanced LFIC for VCP detection Biotinylated gold nanoparticles tethered with streptavidin-bearing HRP at the capture site in the lateral flow strip to generate a signal upon interaction with TMB.

    Article Snippet: This was followed by the application of biotinylated goat anti-mouse secondary antibody (GAM IgG) conjugated to HRP (Dakocytomation, Carpinteria, CA).

    Techniques: Flow Cytometry, Stripping Membranes