Journal: Experimental and Therapeutic Medicine

Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

doi: 10.3892/etm.2015.2961

Figure Lengend Snippet: Comparison of the morphology of Bio-ADSCs on Avi-β-TCP and untreated ADSCs on β-TCP. Scanning electron microscopy micrographs of (A) ADSCs/β-TCP and (B) Bio-ADSCs/Avi-β-TCP constructs following 7 day incubation (magnification, ×1,200). ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated ADSCs; Avi-β-TCP, avidin-coated β-TCP.

Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

Techniques: Electron Microscopy, Construct, Incubation, Derivative Assay, Avidin-Biotin Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

doi: 10.3892/etm.2015.2961

Figure Lengend Snippet: (A and B) Hematoxylin and eosin staining and (C and D) immunohistochemical analysis of osteocalcin protein expression at the mandibular defect area in the (A and C) group 1 and (B and D) group 2 rabbits at 4 weeks post-operation (magnification, ×200). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

Techniques: Staining, Immunohistochemistry, Expressing, Construct, Derivative Assay, Avidin-Biotin Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Biotin-avidin mediates the binding of adipose-derived stem cells to a porous β-tricalcium phosphate scaffold: Mandibular regeneration

doi: 10.3892/etm.2015.2961

Figure Lengend Snippet: Three-dimensional computed tomography images of mandibular defect reconstruction in the (A) group 1 and (B) group 2 rabbits at 4 weeks post-operation (red arrow). The group 1 and group 2 rabbits received the Bio-ADSCs/Avi-β-TCP and Bio-ADSCs/Avi-β-TCP/PRP constructs, respectively. ADSCs, adipose-derived stem cells; β-TCP, β-tricalcium phosphate; Bio-ADSCs, biotinylated-ADSCs; Avi-β-TCP, avidin-coated β-TCP; PRP, platelet-rich plasma.

Article Snippet: After rinsing in PBS three times, the tissue sections were incubated with biotinylated secondary antibody (1:500; Z0420; DAKO, Glostrup, Denmark) at room temperature for 1 h. Peroxidase activity was visualized using 0.05% diaminobenzidine and 0.03% H2O2 in PBS.

Techniques: Computed Tomography, Construct, Derivative Assay, Avidin-Biotin Assay

Journal: Medicine

Article Title: Validation of Serological Antibody Profiles Against Human Papillomavirus Type 16 Antigens as Markers for Early Detection of Cervical Cancer

doi: 10.1097/MD.0000000000002769

Figure Lengend Snippet: Seropositivity to E4, E7, and VLPs-L1 in serum of healthy women and women with CC by Slot blot system. In vitro purified HPV antigens were immobilized in PROTRAN membranes using the Hybri-Slot system, and membranes were fixed and blocked as described in “Materials and Methods.” Strips containing the 3 HPV16 antigens (E4 10 ng, E7 10 ng, VLPs-L1 200 ng) and negative controls (buffer alone and GFP 200 ng) were incubated with dilutions (1:2,500) of sera from women with CC (A), sera from healthy women (B), and positive and negative female serum controls for anti-HPV antibodies (C). Biotinylated secondary antibody (dil 1:10,000) and Streptavidin-horseradish peroxidase (1:8,000) were used to develop the system, and the bands were finally visualized by chemiluminescence in the Odyssey Fc system. CC = cervical cancer, GFP = green fluorescent protein, HPV = human papillomavirus, VLPs-L1 = virus-like particles from L1 protein.

Article Snippet: The biotinylated secondary antibody (goat anti-human immunoglobulins; dilution 1:10,000) (Jackson Immunoresearch Laboratories, USA) was diluted in blocking solution and incubated at 4o C for 1.5 hours.

Techniques: Dot Blot, In Vitro, Purification, Incubation