biotinylated rabbit igg Millipore Search Results


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  • 95
    Vector Laboratories affinity purified biotinylated rabbit anti goat igg
    Affinity Purified Biotinylated Rabbit Anti Goat Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity purified biotinylated rabbit anti goat igg/product/Vector Laboratories
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
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    92
    Millipore anti rabbit igg gamma chain specific biotin antibody mouse monoclonal
    Anti Rabbit Igg Gamma Chain Specific Biotin Antibody Mouse Monoclonal, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 12 article reviews
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    82
    Millipore biotinylated total rabbit igg
    Biotinylated Total Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 16 article reviews
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    81
    Millipore biotinylated rabbit antigoat igg
    DDI of capture antibodies from goat directed against human <t>IgG</t> (GAH). Immobilization efficiencies of three alternative techniques are compared: <t>biotinylated</t> GAH was immobilized by either direct physisorption (triangles), biotin–STV interaction (rectangles) or DDI (circles). Serial dilutions of the target antigen (human IgG, hIgG) were incubated in the wells containing the capture antibody. Signal detection was carried out by either regular ELISA ( A ), using an anti-hIgG–STV–alkaline phosphatase conjugate and fluorescence detection or IPCR ( B ) using an anti-human IgG-STV-DNA conjugate and real-time TaqMan detection. The standard deviation of duplicate measurements was
    Biotinylated Rabbit Antigoat Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 81 stars, based on 119 article reviews
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    99
    Millipore biotinylated anti rabbit igg
    Enhanced binding of HSV-1 gD:Fc and PRV-gD:Fc to MDCK cells and MDCK-nectin-1 cells after a switch to LC medium. Cells grown in 96-well plates were maintained in NC medium or switched to LC medium for 2 h and incubated with either HSV-1 gD:Fc or PRV gD:Fc for 1 h. Cells were then washed, fixed, and sequentially incubated with <t>biotinylated</t> goat anti-rabbit <t>IgG,</t> streptavidin-conjugated HRP, and HRP substrate. Values are optical densities at 370 nm; means of triplicate determinations with standard deviations for one representative experiment of five replicates are shown.
    Biotinylated Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore biotinylated rabbit anti rat igg
    Enhanced binding of HSV-1 gD:Fc and PRV-gD:Fc to MDCK cells and MDCK-nectin-1 cells after a switch to LC medium. Cells grown in 96-well plates were maintained in NC medium or switched to LC medium for 2 h and incubated with either HSV-1 gD:Fc or PRV gD:Fc for 1 h. Cells were then washed, fixed, and sequentially incubated with <t>biotinylated</t> goat anti-rabbit <t>IgG,</t> streptavidin-conjugated HRP, and HRP substrate. Values are optical densities at 370 nm; means of triplicate determinations with standard deviations for one representative experiment of five replicates are shown.
    Biotinylated Rabbit Anti Rat Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore biotinylated donkey anti rabbit igg
    Enhanced binding of HSV-1 gD:Fc and PRV-gD:Fc to MDCK cells and MDCK-nectin-1 cells after a switch to LC medium. Cells grown in 96-well plates were maintained in NC medium or switched to LC medium for 2 h and incubated with either HSV-1 gD:Fc or PRV gD:Fc for 1 h. Cells were then washed, fixed, and sequentially incubated with <t>biotinylated</t> goat anti-rabbit <t>IgG,</t> streptavidin-conjugated HRP, and HRP substrate. Values are optical densities at 370 nm; means of triplicate determinations with standard deviations for one representative experiment of five replicates are shown.
    Biotinylated Donkey Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Millipore biotinylated sheep anti rabbit igg
    Enhanced binding of HSV-1 gD:Fc and PRV-gD:Fc to MDCK cells and MDCK-nectin-1 cells after a switch to LC medium. Cells grown in 96-well plates were maintained in NC medium or switched to LC medium for 2 h and incubated with either HSV-1 gD:Fc or PRV gD:Fc for 1 h. Cells were then washed, fixed, and sequentially incubated with <t>biotinylated</t> goat anti-rabbit <t>IgG,</t> streptavidin-conjugated HRP, and HRP substrate. Values are optical densities at 370 nm; means of triplicate determinations with standard deviations for one representative experiment of five replicates are shown.
    Biotinylated Sheep Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Millipore biotinylated rabbit anti human igg
    Enhanced binding of HSV-1 gD:Fc and PRV-gD:Fc to MDCK cells and MDCK-nectin-1 cells after a switch to LC medium. Cells grown in 96-well plates were maintained in NC medium or switched to LC medium for 2 h and incubated with either HSV-1 gD:Fc or PRV gD:Fc for 1 h. Cells were then washed, fixed, and sequentially incubated with <t>biotinylated</t> goat anti-rabbit <t>IgG,</t> streptavidin-conjugated HRP, and HRP substrate. Values are optical densities at 370 nm; means of triplicate determinations with standard deviations for one representative experiment of five replicates are shown.
    Biotinylated Rabbit Anti Human Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 1 article reviews
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    77
    Millipore biotinylated mouse anti rabbit igg antibody
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Mouse Anti Rabbit Igg Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore biotinylated anti mouse rat rabbit igg
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Anti Mouse Rat Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore biotinylated goat anti rabbit igg solution
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Goat Anti Rabbit Igg Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Millipore biotinylated monoclonal anti rabbit igg
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Monoclonal Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 9 article reviews
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    85
    Millipore biotinylated swine anti rabbit igg
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Swine Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore biotinylated secondary antisera against rabbit igg
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Secondary Antisera Against Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Millipore biotinylated anti mouse goat rat rabbit igg
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Anti Mouse Goat Rat Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Vector Laboratories biotinylated goat anti rabbit antibodies
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Goat Anti Rabbit Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore biotinylated anti mouse goat rabbit igg
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Anti Mouse Goat Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore anti rabbit igg whole molecule biotin antibody
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Anti Rabbit Igg Whole Molecule Biotin Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore biotinylated goat anti rabbit igg serum
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Goat Anti Rabbit Igg Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore biotinylated goat antiserum against rabbit iggs
    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the <t>biotinylated</t> detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse <t>IgG;</t> Neg.Co. = BSA.
    Biotinylated Goat Antiserum Against Rabbit Iggs, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore igg from rabbit serum
    <t>M4P</t> improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit <t>IgG</t> in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p
    Igg From Rabbit Serum, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore affinity purified biotinylated goat anti rabbit igg secondary antibody
    <t>M4P</t> improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit <t>IgG</t> in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p
    Affinity Purified Biotinylated Goat Anti Rabbit Igg Secondary Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotinylated mouse anti rabbit igg hrp sc 2357 antibody
    <t>M4P</t> improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit <t>IgG</t> in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p
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    Millipore donkey anti rabbit igg
    <t>M4P</t> improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit <t>IgG</t> in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p
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    Millipore monoclonal anti human igg1
    <t>M4P</t> improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit <t>IgG</t> in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p
    Monoclonal Anti Human Igg1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore goat anti rabbit igg biotinylated species specific whole antibody
    <t>M4P</t> improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit <t>IgG</t> in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p
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    Image Search Results


    DDI of capture antibodies from goat directed against human IgG (GAH). Immobilization efficiencies of three alternative techniques are compared: biotinylated GAH was immobilized by either direct physisorption (triangles), biotin–STV interaction (rectangles) or DDI (circles). Serial dilutions of the target antigen (human IgG, hIgG) were incubated in the wells containing the capture antibody. Signal detection was carried out by either regular ELISA ( A ), using an anti-hIgG–STV–alkaline phosphatase conjugate and fluorescence detection or IPCR ( B ) using an anti-human IgG-STV-DNA conjugate and real-time TaqMan detection. The standard deviation of duplicate measurements was

    Journal: Nucleic Acids Research

    Article Title: Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA-protein conjugates

    doi:

    Figure Lengend Snippet: DDI of capture antibodies from goat directed against human IgG (GAH). Immobilization efficiencies of three alternative techniques are compared: biotinylated GAH was immobilized by either direct physisorption (triangles), biotin–STV interaction (rectangles) or DDI (circles). Serial dilutions of the target antigen (human IgG, hIgG) were incubated in the wells containing the capture antibody. Signal detection was carried out by either regular ELISA ( A ), using an anti-hIgG–STV–alkaline phosphatase conjugate and fluorescence detection or IPCR ( B ) using an anti-human IgG-STV-DNA conjugate and real-time TaqMan detection. The standard deviation of duplicate measurements was

    Article Snippet: Preconjugates of HA24 and biotinylated antibodies were prepared by mixing 0.01 mM stock solutions of HA24 and equimolar amounts (0.01 mM stock solution) of biotinylated goat anti-rabbit IgG (Sigma), biotinylated goat anti-human IgG (Biotrend) or biotinylated rabbit anti-CEA (Dako) [biotinylated with NHS-Biotin (Pierce) according to the manufacturer’s instructions] in buffer A (10 mM Tris buffer, pH 7.5, containing 5 mM EDTA).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Fluorescence, Standard Deviation

    NDRG2 interacts with GLUT1. (A) SK-BR-3 cells were fixed and incubated with primary antibodies against N-myc downstream-regulated gene 2 (NDRG2) or glucose transporter 1 (GLUT1) and with fluorescein isothiocyanate or a cyanine 3 secondary antibody. Green fluorescence indicates NDRG2 expression, red fluorescence indicates GLUT1 expression and blue fluorescence indicates nuclear staining. The results of the merged images reveal that NDRG2 and GLUT1 were colocalised in the cytoplasm. (B) Immunoprecipitation (IP) assays were performed with whole-cell lysates of SK-BR-3 cells pretreated with protein A–conjugated sepharose beads. Whole-cell lysates were probed for input. The antibodies for immunoprecipitation and Western blot (WB) analyses were carried out as indicated. The locations of various proteins are indicated by arrowheads. IgG, Immunoglobulin G.

    Journal: Breast Cancer Research : BCR

    Article Title: N-myc downstream-regulated gene 2 expression is associated with glucose transport and correlated with prognosis in breast carcinoma

    doi: 10.1186/bcr3628

    Figure Lengend Snippet: NDRG2 interacts with GLUT1. (A) SK-BR-3 cells were fixed and incubated with primary antibodies against N-myc downstream-regulated gene 2 (NDRG2) or glucose transporter 1 (GLUT1) and with fluorescein isothiocyanate or a cyanine 3 secondary antibody. Green fluorescence indicates NDRG2 expression, red fluorescence indicates GLUT1 expression and blue fluorescence indicates nuclear staining. The results of the merged images reveal that NDRG2 and GLUT1 were colocalised in the cytoplasm. (B) Immunoprecipitation (IP) assays were performed with whole-cell lysates of SK-BR-3 cells pretreated with protein A–conjugated sepharose beads. Whole-cell lysates were probed for input. The antibodies for immunoprecipitation and Western blot (WB) analyses were carried out as indicated. The locations of various proteins are indicated by arrowheads. IgG, Immunoglobulin G.

    Article Snippet: Biotinylated goat anti-rabbit immunoglobulin G (IgG) (1:400; Sigma-Aldrich, St Louis, MO, USA) was incubated with the sections for 1 hour at room temperature and detected using a streptavidin-peroxidase complex.

    Techniques: Incubation, Fluorescence, Expressing, Staining, Immunoprecipitation, Western Blot

    Enhanced binding of HSV-1 gD:Fc and PRV-gD:Fc to MDCK cells and MDCK-nectin-1 cells after a switch to LC medium. Cells grown in 96-well plates were maintained in NC medium or switched to LC medium for 2 h and incubated with either HSV-1 gD:Fc or PRV gD:Fc for 1 h. Cells were then washed, fixed, and sequentially incubated with biotinylated goat anti-rabbit IgG, streptavidin-conjugated HRP, and HRP substrate. Values are optical densities at 370 nm; means of triplicate determinations with standard deviations for one representative experiment of five replicates are shown.

    Journal: Journal of Virology

    Article Title: Disruption of Adherens Junctions Liberates Nectin-1 To Serve as Receptor for Herpes Simplex Virus and Pseudorabies Virus Entry

    doi: 10.1128/JVI.76.14.7203-7208.2002

    Figure Lengend Snippet: Enhanced binding of HSV-1 gD:Fc and PRV-gD:Fc to MDCK cells and MDCK-nectin-1 cells after a switch to LC medium. Cells grown in 96-well plates were maintained in NC medium or switched to LC medium for 2 h and incubated with either HSV-1 gD:Fc or PRV gD:Fc for 1 h. Cells were then washed, fixed, and sequentially incubated with biotinylated goat anti-rabbit IgG, streptavidin-conjugated HRP, and HRP substrate. Values are optical densities at 370 nm; means of triplicate determinations with standard deviations for one representative experiment of five replicates are shown.

    Article Snippet: Cells were washed, fixed with 2% formaldehyde and 0.2% glutaraldehyde, and sequentially incubated with biotinylated anti-rabbit IgG (Sigma), Amdex streptavidin-conjugated horseradish peroxidase (HRP; Amersham), and HRP substrate (BioFx Lab).

    Techniques: Binding Assay, Incubation

    Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the biotinylated detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse IgG; Neg.Co. = BSA.

    Journal: Frontiers in Chemistry

    Article Title: Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins

    doi: 10.3389/fchem.2019.00031

    Figure Lengend Snippet: Illustrated principle of the competitive, anti-idiotypic antibody-based biochip assays with amperometric measurement. (A) The electrical biochip exhibits 16 IDA gold electrodes for immobilization of capture molecules. Utilization of anti-toxin mAbs and anti-idiotypic mAbs as capture or detector enabling two different assay layouts (I and II) based on a competitive format. During an automatic measurement the sample (toxin) plus the biotinylated detector antibody, the streptavidin labeled β-D-galactosidase and the substrate p -APG are sequentially applied to the biochip. Signal amplification of electrochemical detection is realized via redox cycling of p -AP between anodic and cathodic fingers of the electrode. (B) Amperometric response curves (current vs. time) were generated after substrate flow to the biochip followed by enzymatic conversion to the electrochemically active product and redox cycling in stopped-flow mode. Curves for 10 and 100 ng/ml STX were obtained and compiled from two independent biochips. Pos.Co. = Biotin labeled rabbit anti-mouse IgG; Neg.Co. = BSA.

    Article Snippet: Biotinylated rabbit anti-mouse IgG (Sigma-Aldrich GmbH, Taufkirchen, Germany) was used as positive control (2.5 μg/mL in 0.02% (w/v) BSA/PBS) and 400 μg/mL BSA/PBS as negative control.

    Techniques: Labeling, Amplification, Generated, Flow Cytometry

    (A and B) Anti-mHSP70 autoantibodies as well as anti-TgHSP70 antibodies were produced in T. gondii -infected BALB/c and B6 mice. BALB/c and B6 mice were perorally infected with five T. gondii cysts of the Fukaya strain. By using rmHSP70 and rTgHSP70 protein as antigens, formation of anti-mHSP70 autoantibody (A) and anti-TgHSP70 antibody (B) in the sera of infected BALB/c and B6 mice was tested weekly by ELISA. (C and D) Isotype specificity of anti-mHSP70 autoantibody generated by T. gondii -infected mice. BALB/c and B6 mice were perorally infected with five T. gondii cysts of the Fukaya strain. Production of anti-mHSP70 IgG autoantibodies (C) or anti-mHSP70 IgM autoantibodies (D) in the sera of T. gondii -infected BALB/c and B6 mice was tested weekly by ELISA using alkaline phosphatase-conjugated anti-mouse IgG antibody or anti-mouse IgM antibody as the secondary antibody. (E and F) Titration analysis of anti-mHSP70 autoantibody and anti-TgHSP70 antibody in the sera of T. gondii -infected BALB/c and B6 mice. After dilution of the sera of BALB/c and B6 mice 5 weeks after T. gondii infection as described in Materials and Methods, the titration of anti-mHSP70 autoantibody (E) and anti-TgHSP70 antibody (F) was analyzed by ELISA. Symbols: □, anti-mHSP70 autoantibodies in BALB/c mice; ■, anti-mHSP70 autoantibodies in B6 mice; ○, anti-TgHSP70 antibodies in BALB/c mice; ●, anti-TgHSP70 antibodies in B6 mice. ∗, P

    Journal: Infection and Immunity

    Article Title: Anti-HSP70 Autoantibody Formation by B-1 Cells in Toxoplasma gondii-Infected Mice

    doi:

    Figure Lengend Snippet: (A and B) Anti-mHSP70 autoantibodies as well as anti-TgHSP70 antibodies were produced in T. gondii -infected BALB/c and B6 mice. BALB/c and B6 mice were perorally infected with five T. gondii cysts of the Fukaya strain. By using rmHSP70 and rTgHSP70 protein as antigens, formation of anti-mHSP70 autoantibody (A) and anti-TgHSP70 antibody (B) in the sera of infected BALB/c and B6 mice was tested weekly by ELISA. (C and D) Isotype specificity of anti-mHSP70 autoantibody generated by T. gondii -infected mice. BALB/c and B6 mice were perorally infected with five T. gondii cysts of the Fukaya strain. Production of anti-mHSP70 IgG autoantibodies (C) or anti-mHSP70 IgM autoantibodies (D) in the sera of T. gondii -infected BALB/c and B6 mice was tested weekly by ELISA using alkaline phosphatase-conjugated anti-mouse IgG antibody or anti-mouse IgM antibody as the secondary antibody. (E and F) Titration analysis of anti-mHSP70 autoantibody and anti-TgHSP70 antibody in the sera of T. gondii -infected BALB/c and B6 mice. After dilution of the sera of BALB/c and B6 mice 5 weeks after T. gondii infection as described in Materials and Methods, the titration of anti-mHSP70 autoantibody (E) and anti-TgHSP70 antibody (F) was analyzed by ELISA. Symbols: □, anti-mHSP70 autoantibodies in BALB/c mice; ■, anti-mHSP70 autoantibodies in B6 mice; ○, anti-TgHSP70 antibodies in BALB/c mice; ●, anti-TgHSP70 antibodies in B6 mice. ∗, P

    Article Snippet: Blots were blocked with 10% skim milk in Tris-buffered saline (pH 7.6) containing 0.1% Tween 20 (TBST), probed with anti-TgHSP70 monoclonal antibody (MAb) for 2 h, washed four times in TBST, incubated with biotinylated rabbit anti-mouse immunoglobulin G (IgG) antibody (Sigma Biosciences, St. Louis, Mo.) diluted 1:1,000 for 2 h, and incubated with horseradish peroxidase-conjugated streptavidin (Sigma) diluted 1:1,000 for 30 min.

    Techniques: Produced, Infection, Mouse Assay, Enzyme-linked Immunosorbent Assay, Generated, Titration

    M4P improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit IgG in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p

    Journal: Pflugers Archiv

    Article Title: TRPM4-specific blocking antibody attenuates reperfusion injury in a rat model of stroke

    doi: 10.1007/s00424-019-02326-8

    Figure Lengend Snippet: M4P improves BBB integrity after stroke reperfusion. a Extravasation of M4P or control rabbit IgG in rat brains. The experimental protocol is similar as in Fig. 4b . Immunostaining was performed 1 day after stroke reperfusion. Fluorescent intensities from 3 experiments were quantified on right. b Western blot using anti-rabbit IgG to detect antibody extravasation for control rabbit IgG and M4P rabbit polyclonal antibodies. Heavy chain (50 kDa) and light chain (25 kDa) were labelled. Antibody extravasations (both heavy and light chains) were summarised from 5 experiments (normalised to actin). c Evans blue extravasations were performed 1 day after stroke reperfusion to evaluate BBB integrity. The dye in the ipsilateral hemispheres (left) was normalised to that in the contralateral hemispheres (right). n = 5 rats/group. In a and b , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. In c , statistical analysis was performed by two-tailed unpaired Student’s t test. * p

    Article Snippet: A single dose of 100 μg of M4P antibody [ ] or control rabbit IgG (I5006, Sigma-Aldrich, MI, USA) was injected intravenously via tail vein at 2 h after occlusion (1 h before recanalization).

    Techniques: Immunostaining, Western Blot, Two Tailed Test

    M4P treatment ameliorates vascular damage and improves functional recovery after stroke reperfusion. a Western blot detecting TRPM4 expression in sham-operated rats and MCAO rats treated with IgG or M4P. Tissue lysates were prepared from penumbra regions in ipsilateral hemispheres of control IgG or M4P treated MCAO rat (Ips) and symmetrical regions in contralateral hemispheres (Con), and from similar regions of sham-operated rat brain (Sham). n = 4. b The blood vessels within the ipsilateral hemispheres 1 day after reperfusion was stained by anti-vWF antibody. Feret vascular diameter was quantified and summarised (17 images per group from 3 experiments). c Assessment of motor functions by Rotarod test ( n = 8). In a , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis; in b , by two-tailed unpaired Student’s t test; in c , by two-way ANOVA with Bonferroni’s post hoc analysis. *** p

    Journal: Pflugers Archiv

    Article Title: TRPM4-specific blocking antibody attenuates reperfusion injury in a rat model of stroke

    doi: 10.1007/s00424-019-02326-8

    Figure Lengend Snippet: M4P treatment ameliorates vascular damage and improves functional recovery after stroke reperfusion. a Western blot detecting TRPM4 expression in sham-operated rats and MCAO rats treated with IgG or M4P. Tissue lysates were prepared from penumbra regions in ipsilateral hemispheres of control IgG or M4P treated MCAO rat (Ips) and symmetrical regions in contralateral hemispheres (Con), and from similar regions of sham-operated rat brain (Sham). n = 4. b The blood vessels within the ipsilateral hemispheres 1 day after reperfusion was stained by anti-vWF antibody. Feret vascular diameter was quantified and summarised (17 images per group from 3 experiments). c Assessment of motor functions by Rotarod test ( n = 8). In a , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis; in b , by two-tailed unpaired Student’s t test; in c , by two-way ANOVA with Bonferroni’s post hoc analysis. *** p

    Article Snippet: A single dose of 100 μg of M4P antibody [ ] or control rabbit IgG (I5006, Sigma-Aldrich, MI, USA) was injected intravenously via tail vein at 2 h after occlusion (1 h before recanalization).

    Techniques: Functional Assay, Western Blot, Expressing, Staining, Two Tailed Test

    Generation and characterisation of TRPM4 blocking antibody M4P. a Schematic representation of TRPM4 channel with the antigenic epitope for M4P labelled in red. b Using M4P to detect TRPM4 channel in HEK 293 cells transfected with mouse TRPM4 (+) or an empty vector (−). c Live HEK 293 cells with TRPM4 expression were incubated with 1.3 μg/ml M4P for 30 min, 3 h and 2 days, and fixed for immunostaining to detect M4P localization. Scale bar 50 μm. d Detection and quantification of TRPM4 channel expression. TRPM4 transfected HEK 293 cells were incubated with rabbit IgG (1.3 μg/ml) and M4P (1.3 μg/ml) for 6 h, then surface biotinylation assay was performed. Con: control group received no treatment. For quantification, n = 4 experiments, and statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. * p

    Journal: Pflugers Archiv

    Article Title: TRPM4-specific blocking antibody attenuates reperfusion injury in a rat model of stroke

    doi: 10.1007/s00424-019-02326-8

    Figure Lengend Snippet: Generation and characterisation of TRPM4 blocking antibody M4P. a Schematic representation of TRPM4 channel with the antigenic epitope for M4P labelled in red. b Using M4P to detect TRPM4 channel in HEK 293 cells transfected with mouse TRPM4 (+) or an empty vector (−). c Live HEK 293 cells with TRPM4 expression were incubated with 1.3 μg/ml M4P for 30 min, 3 h and 2 days, and fixed for immunostaining to detect M4P localization. Scale bar 50 μm. d Detection and quantification of TRPM4 channel expression. TRPM4 transfected HEK 293 cells were incubated with rabbit IgG (1.3 μg/ml) and M4P (1.3 μg/ml) for 6 h, then surface biotinylation assay was performed. Con: control group received no treatment. For quantification, n = 4 experiments, and statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis. * p

    Article Snippet: A single dose of 100 μg of M4P antibody [ ] or control rabbit IgG (I5006, Sigma-Aldrich, MI, USA) was injected intravenously via tail vein at 2 h after occlusion (1 h before recanalization).

    Techniques: Blocking Assay, Transfection, Plasmid Preparation, Expressing, Incubation, Immunostaining, Surface Biotinylation Assay

    M4P blocks TRPM4 channel currents. a Time course of normalised currents by membrane capacitance at + 100 mV and − 100 mV from ramp protocols applied from − 100 to + 100 mV. The pipette solution contained a calculated 7.4 μM free Ca 2+ . The current-voltage relationships before desensitisation were presented as means ± s.e.m. in the middle panel. Summary of current densities at − 100 mV and 100 mV before desensitisation is shown on the right. Data were obtained from 19 cells for IgG and 15 cells for M4P. b Current-voltage relationship for M4P ( n = 10) and rabbit IgG ( n = 12) treatments from 250-ms voltage ramps after 1 min (left panel) and 7 min (middle panel) hypoxia treatment. Summary of current at − 100 mV and 100 mV under the same conditions is shown on the right. c Normalised current by membrane capacitance (C m ) of M4P or rabbit IgG-treated cells after 1 min and 7 min hypoxia treatment. d Time course of membrane capacitance (C m ) changes with IgG ( n = 12) or M4P ( n = 10) treatment under hypoxic conditions. HEK 293 cells ( n = 13) under normoxic conditions were recorded as control. No difference was observed between M4P and control groups. e Summary of cell death after 24 h OGD with the treatment of M4P or IgG. n = 4 experiments. For a – d , TRPM4-transfected HEK 293 cells were incubated with 20.8 μg/ml IgG or M4P for 30 min before patch-clamping. In a , b , c , and e , statistical analysis was performed by two-tailed unpaired Student’s t test; in d by two-way ANOVA with Bonferroni’s post hoc analysis. * p

    Journal: Pflugers Archiv

    Article Title: TRPM4-specific blocking antibody attenuates reperfusion injury in a rat model of stroke

    doi: 10.1007/s00424-019-02326-8

    Figure Lengend Snippet: M4P blocks TRPM4 channel currents. a Time course of normalised currents by membrane capacitance at + 100 mV and − 100 mV from ramp protocols applied from − 100 to + 100 mV. The pipette solution contained a calculated 7.4 μM free Ca 2+ . The current-voltage relationships before desensitisation were presented as means ± s.e.m. in the middle panel. Summary of current densities at − 100 mV and 100 mV before desensitisation is shown on the right. Data were obtained from 19 cells for IgG and 15 cells for M4P. b Current-voltage relationship for M4P ( n = 10) and rabbit IgG ( n = 12) treatments from 250-ms voltage ramps after 1 min (left panel) and 7 min (middle panel) hypoxia treatment. Summary of current at − 100 mV and 100 mV under the same conditions is shown on the right. c Normalised current by membrane capacitance (C m ) of M4P or rabbit IgG-treated cells after 1 min and 7 min hypoxia treatment. d Time course of membrane capacitance (C m ) changes with IgG ( n = 12) or M4P ( n = 10) treatment under hypoxic conditions. HEK 293 cells ( n = 13) under normoxic conditions were recorded as control. No difference was observed between M4P and control groups. e Summary of cell death after 24 h OGD with the treatment of M4P or IgG. n = 4 experiments. For a – d , TRPM4-transfected HEK 293 cells were incubated with 20.8 μg/ml IgG or M4P for 30 min before patch-clamping. In a , b , c , and e , statistical analysis was performed by two-tailed unpaired Student’s t test; in d by two-way ANOVA with Bonferroni’s post hoc analysis. * p

    Article Snippet: A single dose of 100 μg of M4P antibody [ ] or control rabbit IgG (I5006, Sigma-Aldrich, MI, USA) was injected intravenously via tail vein at 2 h after occlusion (1 h before recanalization).

    Techniques: Transferring, Mass Spectrometry, Transfection, Incubation, Two Tailed Test

    M4P application improves stroke outcome after reperfusion. a Immunostaining of ipsilateral hemispheres and the summary of the fluorescent intensity (19–28 images per group from 3 experiments). Vehicle, control rabbit IgG (100 μg), or M4P (100 μg) was injected 2 h after stroke induction. At 3 h, rat brains were collected and sectioned for immunostaining using secondary antibodies against rabbit IgG. Summary of the fluorescent intensity (19–28 images per group from 3 experiments) is shown on the right. b Experimental protocol of a 3-h MCAO followed by reperfusion. M4P (100 μg) or control rabbit IgG (100 μg) was administered intravenously 1 h prior recanalization. Mortality rates are shown (lower panel). c TTC staining of rat brains 1 day after operation. d The summary of total infarct volume ( n = 6). e Section-by-section infarct area distribution ( n = 6). In a , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis; in b , by Fisher exact probability test; in d and e , by Student’s t test. * p

    Journal: Pflugers Archiv

    Article Title: TRPM4-specific blocking antibody attenuates reperfusion injury in a rat model of stroke

    doi: 10.1007/s00424-019-02326-8

    Figure Lengend Snippet: M4P application improves stroke outcome after reperfusion. a Immunostaining of ipsilateral hemispheres and the summary of the fluorescent intensity (19–28 images per group from 3 experiments). Vehicle, control rabbit IgG (100 μg), or M4P (100 μg) was injected 2 h after stroke induction. At 3 h, rat brains were collected and sectioned for immunostaining using secondary antibodies against rabbit IgG. Summary of the fluorescent intensity (19–28 images per group from 3 experiments) is shown on the right. b Experimental protocol of a 3-h MCAO followed by reperfusion. M4P (100 μg) or control rabbit IgG (100 μg) was administered intravenously 1 h prior recanalization. Mortality rates are shown (lower panel). c TTC staining of rat brains 1 day after operation. d The summary of total infarct volume ( n = 6). e Section-by-section infarct area distribution ( n = 6). In a , statistical analysis was performed by one-way ANOVA with Bonferroni’s post hoc analysis; in b , by Fisher exact probability test; in d and e , by Student’s t test. * p

    Article Snippet: A single dose of 100 μg of M4P antibody [ ] or control rabbit IgG (I5006, Sigma-Aldrich, MI, USA) was injected intravenously via tail vein at 2 h after occlusion (1 h before recanalization).

    Techniques: Immunostaining, Injection, Staining