biotinylated mouse f Search Results


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  • 99
    Vector Laboratories biotinylated goat anti mouse
    Biotinylated Goat Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1005 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat anti mouse/product/Vector Laboratories
    Average 99 stars, based on 1005 article reviews
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    biotinylated goat anti mouse - by Bioz Stars, 2020-08
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    80
    Agilent technologies biotinylated anti mouse f
    Biotinylated Anti Mouse F, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 9 article reviews
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    85
    Jackson Immuno biotinylated anti mouse f
    Biotinylated Anti Mouse F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotinylated goat anti mouse f
    Biotinylated Goat Anti Mouse F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 16 article reviews
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    85
    Biogenex biotinylated anti mouse f
    Biotinylated Anti Mouse F, supplied by Biogenex, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Jackson Immuno biotinylated goat anti mouse igg f
    Biotinylated Goat Anti Mouse Igg F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies biotinylated rabbit anti mouse immunoglobulin f
    Biotinylated Rabbit Anti Mouse Immunoglobulin F, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Accurate Chemical & Scientific Corporation biotinylated anti mouse f
    Biotinylated Anti Mouse F, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Jackson Immuno biotinylated donkey anti mouse igg f
    Biotinylated Donkey Anti Mouse Igg F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Valiant biotinylated goat anti mouse igm f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated Goat Anti Mouse Igm F, supplied by Valiant, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Jackson Immuno biotinylated f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 240 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated f - by Bioz Stars, 2020-08
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    90
    Becton Dickinson biotinylated f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated F, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Accurate Chemical & Scientific Corporation biotinylated rabbit anti mouse f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated Rabbit Anti Mouse F, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies biotinylated f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated F, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    GE Healthcare biotinylated f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated F, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Abcam biotinylated goat α mouse ig f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated Goat α Mouse Ig F, supplied by Abcam, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies biotinylated rabbit anti mouse f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated Rabbit Anti Mouse F, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies biotinylated rabbit anti mouse secondary antibody igg f
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated Rabbit Anti Mouse Secondary Antibody Igg F, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Becton Dickinson mouse siglec f biotinylated antibody
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Mouse Siglec F Biotinylated Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse siglec f biotinylated antibody/product/Becton Dickinson
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    88
    Millipore biotinylated anti mouse cd11b
    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
    Biotinylated Anti Mouse Cd11b, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with <t>biotinylated</t> anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p
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    PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with biotinylated anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p

    Journal: Molecular immunology

    Article Title: CD19 signaling is impaired in murine peritoneal and splenic B-1 B lymphocytes

    doi: 10.1016/j.molimm.2009.04.015

    Figure Lengend Snippet: PP1 restores the BCR + CD19 signaling defect in B-1 cells (A) Peritoneal cells were loaded with Indo-1 and stained with α-B220 and α-Mac-1/CD11b as described in the Methods, treated with biotinylated anti-IgM and biotinylated anti-CD19; pre-incubated with PP1 at room temperature for 20 min prior to obtaining the 30 sec baseline. Avidin was added as indicated by the arrow. Graph represents one of three independent measurements. (B) C57BL/6 splenocytes were Indo-1 loaded, stained with anti-B220~PE and anti-CD43~FITC, incubated with biotinylated anti-IgM F(ab′)2 and biotinylated anti-CD19, pre-treated with varying doses of PP1 for 20 min at room temperature, cell suspension warmed to 37°C, activated with avidin and calcium response monitored for 5 min. Shown were the responses of the gated splenic B-1 (B220 + CD43 + ) B cells at two doses of PP1 (μM). (C) Cells from peritoneal lavage were treated as in (A), stained with α-B220~CY, α-Mac-1~PE, α-CD5~FITC, stimulated with biotinylated anti-IgM and biotinylated anti-CD19; pre-treated with PP1 at room temperature for 20 min prior to determining the 30 sec baseline calcium levels. Avidin was added as indicated by the arrow. Graph depicts the responses from gated B-1a (B220 + Mac-1 + CD5 + ) and B-1b (B220 + Mac-1 + CD5 − ) sub-populations. (D) Peritoneal B-1 and V H 12 transgenic splenic B cells (panel E) were treated with 50 μg/ml anti-IgM F (ab′)2 for 48 h in IF-12. The proliferative counts were determined as described in the Methods. Plot represents one of three independent in vitro proliferation assays. p

    Article Snippet: Cells were then stained with anti-B220~PE and anti-CD43~FITC on ice for 30 min along with biotinylated goat anti-mouse IgM F (ab′)2 (MP Biomedicals, Irvine, CA) and anti-CD19 (1D3) antibodies, washed and resuspended in insulin and progesterone free IF-12 media (10% FBS).

    Techniques: Staining, Incubation, Size-exclusion Chromatography, Avidin-Biotin Assay, Transgenic Assay, In Vitro