biotinylated monoclonal anti rabbit igg Search Results


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  • 99
    Vector Laboratories biotinylated anti rabbit igg
    Biotinylated Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 4318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotinylated monoclonal anti rabbit igg
    Biotinylated Monoclonal Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc biotinylated anti rabbit igg
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Biotinylated Anti Rabbit Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cell Signaling Technology Inc anti lc3b rabbit monoclonal igg
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Anti Lc3b Rabbit Monoclonal Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher biotinylated goat anti rabbit igg mab
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Biotinylated Goat Anti Rabbit Igg Mab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Jackson Immuno monoclonal biotinylated donkey anti rabbit igg
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Monoclonal Biotinylated Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Vector Laboratories biotinylated anti igg
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Biotinylated Anti Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Lifespan Biosciences biotinylated anti rabbit igg
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Biotinylated Anti Rabbit Igg, supplied by Lifespan Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc rabbit anti phospho akt monoclonal igg antibody
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Rabbit Anti Phospho Akt Monoclonal Igg Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio antibody biotinylated rabbit anti rat igg
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Antibody Biotinylated Rabbit Anti Rat Igg, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Vector Laboratories biotinylated goat anti rabbit monoclonal antibody
    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) <t>Coimmunoprecipitation</t> of Bcl-xl or the control <t>IgG</t> with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.
    Biotinylated Goat Anti Rabbit Monoclonal Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) Coimmunoprecipitation of Bcl-xl or the control IgG with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.

    Journal: Cell Cycle

    Article Title: Bim directly antagonizes Bcl-xl in doxorubicin-induced prostate cancer cell apoptosis independently of p53

    doi: 10.1080/15384101.2015.1127470

    Figure Lengend Snippet: Bim interacted with Bcl-xl in doxorubicin-induced apoptosis in prostate cancers. (A) Coimmunoprecipitation of Bcl-xl or the control IgG with Bim. Cell lysates from LNCaP or PC3 cells with/without doxorubicin treatment, 0.5 μM for LNCaP and 3 μM for PC3 for 48 hr, were incubated with antibody against Bcl-xl or IgG, and then precipitated with magnetic beads for further immunoblot analysis. Antibodies against Puma and Bim were used for immunoblotting. The Bim co-precipitated with Bcl-xl was indicated by arrow head. Experiments were repeated 3 times and representative results are shown. (B) Bim knockdown reduced doxorubicin-induced apoptosis in LNCaP. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without 0.5 μM doxorubicin treatment were subjected to immunoblotting analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts. (C) Doxorubicin-induced apoptosis is Bim-dependent, whereas ABT-263-induced apoptosis is Bim-independent. Bim and scrambled RNAi knockdown cell lysates from LNCaP cells with/without doxorubicin, ABT-263 or doxorubicin plus ABT-263 treatment were subjected to immunoblot analysis. Experiments were repeated 3 times and representative results are shown. The respective immunoblot image of Bim and caspase 3(a) normalized to GAPDH was quantitated and shown at the right. (**) statistical significance between 2 readouts.

    Article Snippet: About 500 μg of proteins were immunoprecipitated with Bcl-xl antibody (Abcam) or the control IgG at 4o C for 2 h. The control IgG for coimmunoprecipitation was rabbit IgG (Cell Signaling, #4096).

    Techniques: Incubation, Magnetic Beads

    Amer1 interacts with β-arrestin2. A , HEK293T cells were transfected with FLAG-Amer1 and HA-β-arrestin2 individually or together as indicated. Cell lysates were immunoprecipitated ( IP ) with anti-FLAG or anti-HA antibody. Immunoblotting ( WB ) was done with mouse M2 anti-FLAG antibody and with mouse anti-HA antibody. TCL was used as a loading control. B , NB4 cells were stimulated for 2 h with Wnt3a (or control) CM, and cytoplasmic and membrane fractions were harvested. Left panel , NB4 cells are Wnt3a-responsive as shown by the enhanced Lrp6 phosphorylation at Ser-1490. Both Amer1 and β-arrestin2 are isolated preferentially in the cytoplasmic fraction. However, upon Wnt3a stimulation, Amer1 is recruited to the membrane and/or associates more tightly with the membrane and is therefore isolated also in the membrane fraction of NB4 cells. Right panel , Amer1 co-immunoprecipitated with A1CT and A2CT anti β-arrestin antibodies, but not with anti-IgG control antibody and anti-β-arrestin2 cs-3857 antibody, which poorly precipitates endogenous β-arrestin2. C , domain mapping of the Amer1/β-arrestin2 interaction using full-length β-arrestin2 and deletion mutants of Amer1 shows schematic view of Amer1 mutants. The interaction with β-arrestin2 is indicated with +; M1/M2 indicate regions interacting with membrane via PtdIns(4,5)P 2 ; A1/A2/A3 are regions required for the interaction with Apc. Full blots are shown in supplemental Fig. 1, A and B . D , domain mapping of the Amer1/β-arrestin2 interaction uses full-length Amer1 and deletion mutants of β-arrestin2. The mutual interaction is indicated with +. Full blots are shown in supplemental Fig. 1 C . E , the interaction between the C-terminal region of Amer1 (amino acids 942–1135) and the central part of β-arrestin2 is demonstrated by co-immunoprecipitation.

    Journal: The Journal of Biological Chemistry

    Article Title: ?-Arrestin Promotes Wnt-induced Low Density Lipoprotein Receptor-related Protein 6 (Lrp6) Phosphorylation via Increased Membrane Recruitment of Amer1 Protein *

    doi: 10.1074/jbc.M113.498444

    Figure Lengend Snippet: Amer1 interacts with β-arrestin2. A , HEK293T cells were transfected with FLAG-Amer1 and HA-β-arrestin2 individually or together as indicated. Cell lysates were immunoprecipitated ( IP ) with anti-FLAG or anti-HA antibody. Immunoblotting ( WB ) was done with mouse M2 anti-FLAG antibody and with mouse anti-HA antibody. TCL was used as a loading control. B , NB4 cells were stimulated for 2 h with Wnt3a (or control) CM, and cytoplasmic and membrane fractions were harvested. Left panel , NB4 cells are Wnt3a-responsive as shown by the enhanced Lrp6 phosphorylation at Ser-1490. Both Amer1 and β-arrestin2 are isolated preferentially in the cytoplasmic fraction. However, upon Wnt3a stimulation, Amer1 is recruited to the membrane and/or associates more tightly with the membrane and is therefore isolated also in the membrane fraction of NB4 cells. Right panel , Amer1 co-immunoprecipitated with A1CT and A2CT anti β-arrestin antibodies, but not with anti-IgG control antibody and anti-β-arrestin2 cs-3857 antibody, which poorly precipitates endogenous β-arrestin2. C , domain mapping of the Amer1/β-arrestin2 interaction using full-length β-arrestin2 and deletion mutants of Amer1 shows schematic view of Amer1 mutants. The interaction with β-arrestin2 is indicated with +; M1/M2 indicate regions interacting with membrane via PtdIns(4,5)P 2 ; A1/A2/A3 are regions required for the interaction with Apc. Full blots are shown in supplemental Fig. 1, A and B . D , domain mapping of the Amer1/β-arrestin2 interaction uses full-length Amer1 and deletion mutants of β-arrestin2. The mutual interaction is indicated with +. Full blots are shown in supplemental Fig. 1 C . E , the interaction between the C-terminal region of Amer1 (amino acids 942–1135) and the central part of β-arrestin2 is demonstrated by co-immunoprecipitation.

    Article Snippet: The following antibodies were used: mouse anti-FLAG antibody (F1804; Sigma-Aldrich), rat anti-GFP antibody (3H9; Chromotek), mouse HA.11 (MMS-101R; Covance), rabbit anti-HA (ab9110; Abcam), anti-Dvl3 (sc-8027; Santa Cruz Biotechnology), anti-p(S1490)-Lrp6 (2568; Cell Signaling), anti-β-catenin (610153; BD Biosciences), anti-β-actin (sc-1615; Santa Cruz Biotechnology), anti-Myc antibody (sc-40; Santa Cruz Biotechnology), anti-α-catenin (sc-7894; Santa Cruz Biotechnology) mouse anti-Amer1 , rabbit anti-Amer1 (AP17838PU-N; Tocris), rabbit anti-β-arrestin (3857; Cell Signaling), anti-β-arrestin (A1CT and A2CT), a kind gift from R. J. Lefkowitz), rabbit anti-PI4KII (a kind gift from P. De Camilli), and rabbit anti-IgG control antibody (3900; Cell Signaling). siRNA sequences targeting β-arrestin1/2 ( ) and PI4KII ( ) were described earlier.

    Techniques: Transfection, Immunoprecipitation, Western Blot, Isolation