Journal: Molecular Biology of the Cell
Article Title: Progesterone Synthesized by Schwann Cells during Myelin Formation Regulates Neuronal Gene Expression
Figure Lengend Snippet: Immunocytochemistry of the progesterone receptor in Schwann cell/neuronal cocultures. Monoclonal antibodies were applied to Schwann cell/neuronal cocultures and were visualized under light microscopy using the horseradish peroxidase conjugated Vectastain ABC staining kit (Vector Laboratories, Burlingame, CA) and the ImmunoPure Metal Enhanced DAB Substrate ( Pierce , Rockford, IL) The coverslips were then treated with a rat-adsorbed biotinylated antimouse secondary antibody. Antibodies to the progesterone receptor (A) and S-100 (B) were applied to myelinating cocultures (4 days after induction). The arrows indicate the neuronal cell bodies.
Article Snippet: The coverslips were washed in phosphate-buffered saline and treated with a rat adsorbed biotinylated antimouse secondary antibody (Vector Laboratories, Burlingame, CA) for 30 min at 25°C.
Techniques: Immunocytochemistry, Light Microscopy, Staining, Plasmid Preparation