Journal: International Journal of Molecular Sciences
Article Title: Insertion of Basic Amino Acids in the Hemagglutinin Cleavage Site of H4N2 Avian Influenza Virus (AIV)—Reduced Virus Fitness in Chickens is Restored by Reassortment with Highly Pathogenic H5N1 AIV
Figure Lengend Snippet: Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.
Article Snippet: Following sections were used for immunohistochemistry using the avidin–biotin–peroxidase complex method (Vector Laboratories Burlingame, CA, USA) with a primary polyclonal rabbit anti-NP antibody (1:750), and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200) as described [ , ].
Techniques: Immunohistochemistry, Plasmid Preparation