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  • 99
    Vector Laboratories biotinylated goat anti rabbit igg
    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary <t>biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.
    Biotinylated Goat Anti Rabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 9053 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad biotinylated goat anti rabbit igg
    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary <t>biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.
    Biotinylated Goat Anti Rabbit Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    SouthernBiotech biotinylated goat anti rabbit igg
    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary <t>biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.
    Biotinylated Goat Anti Rabbit Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jackson Immuno biotinylated goat anti rabbit igg
    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary <t>biotinylated</t> goat anti-rabbit <t>IgG</t> (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.
    Biotinylated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotinylated goat anti rabbit igg
    DDI of capture antibodies from goat directed against human <t>IgG</t> (GAH). Immobilization efficiencies of three alternative techniques are compared: <t>biotinylated</t> GAH was immobilized by either direct physisorption (triangles), biotin–STV interaction (rectangles) or DDI (circles). Serial dilutions of the target antigen (human IgG, hIgG) were incubated in the wells containing the capture antibody. Signal detection was carried out by either regular ELISA ( A ), using an anti-hIgG–STV–alkaline phosphatase conjugate and fluorescence detection or IPCR ( B ) using an anti-human IgG-STV-DNA conjugate and real-time TaqMan detection. The standard deviation of duplicate measurements was
    Biotinylated Goat Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 595 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies biotinylated goat anti rabbit igg
    Flow cytometric analysis of cell surface expression of IL-18R. ( a ) Th1 (Dorris) and Th2 (D10) were stained with rabbit anti–IL-18R or preimmune serum (1/25 dilution) followed by <t>biotinylated</t> goat anti–rabbit <t>IgG</t> and were developed with PerCP-streptavidin. Similar results were obtained with X4 (Th1) and X12 (Th2) (not shown). ( b ) CD4 + T cells from OVA-TCR-αβ transgenic mice (D011.10) were driven to Th1 or Th2 lines for 6 d with APCs and antigen in the presence of IL-12 and anti–IL-4 antibody (Th1 line) or IL-4 (Th2 line). They were stained for cell surface IL-18R (with PerCP), and intracellular IFN-γ (with FITC) and IL-4 (with PE). All cells in b were activated with PMA/ionomycin for 4 h and Brefeldin A added in the last 2 h. Unfilled histograms , Staining with control preimmune serum. Similar results were obtained with cells driven for up to five rounds of culture.
    Biotinylated Goat Anti Rabbit Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson biotinylated goat anti rabbit igg
    Flow cytometric analysis of cell surface expression of IL-18R. ( a ) Th1 (Dorris) and Th2 (D10) were stained with rabbit anti–IL-18R or preimmune serum (1/25 dilution) followed by <t>biotinylated</t> goat anti–rabbit <t>IgG</t> and were developed with PerCP-streptavidin. Similar results were obtained with X4 (Th1) and X12 (Th2) (not shown). ( b ) CD4 + T cells from OVA-TCR-αβ transgenic mice (D011.10) were driven to Th1 or Th2 lines for 6 d with APCs and antigen in the presence of IL-12 and anti–IL-4 antibody (Th1 line) or IL-4 (Th2 line). They were stained for cell surface IL-18R (with PerCP), and intracellular IFN-γ (with FITC) and IL-4 (with PE). All cells in b were activated with PMA/ionomycin for 4 h and Brefeldin A added in the last 2 h. Unfilled histograms , Staining with control preimmune serum. Similar results were obtained with cells driven for up to five rounds of culture.
    Biotinylated Goat Anti Rabbit Igg, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology biotinylated goat anti rabbit igg
    Flow cytometric analysis of cell surface expression of IL-18R. ( a ) Th1 (Dorris) and Th2 (D10) were stained with rabbit anti–IL-18R or preimmune serum (1/25 dilution) followed by <t>biotinylated</t> goat anti–rabbit <t>IgG</t> and were developed with PerCP-streptavidin. Similar results were obtained with X4 (Th1) and X12 (Th2) (not shown). ( b ) CD4 + T cells from OVA-TCR-αβ transgenic mice (D011.10) were driven to Th1 or Th2 lines for 6 d with APCs and antigen in the presence of IL-12 and anti–IL-4 antibody (Th1 line) or IL-4 (Th2 line). They were stained for cell surface IL-18R (with PerCP), and intracellular IFN-γ (with FITC) and IL-4 (with PE). All cells in b were activated with PMA/ionomycin for 4 h and Brefeldin A added in the last 2 h. Unfilled histograms , Staining with control preimmune serum. Similar results were obtained with cells driven for up to five rounds of culture.
    Biotinylated Goat Anti Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Nichirei biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Nichirei, used in various techniques. Bioz Stars score: 94/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biogenex biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Biogenex, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Zhongshan Golden Bridge Company biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 93/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems goat anti rabbit igg biotinylated antibody
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Goat Anti Rabbit Igg Biotinylated Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biocare Medical biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bioss biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NEN Life Science goat anti rabbit biotinylated igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Goat Anti Rabbit Biotinylated Igg, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 89/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Interchim biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by Interchim, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Jackson Laboratory biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare biotinylated goat anti rabbit igg
    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with <t>biotinylated</t> anti-mouse <t>IgG</t> mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.
    Biotinylated Goat Anti Rabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.

    Journal: International Journal of Molecular Sciences

    Article Title: Insertion of Basic Amino Acids in the Hemagglutinin Cleavage Site of H4N2 Avian Influenza Virus (AIV)—Reduced Virus Fitness in Chickens is Restored by Reassortment with Highly Pathogenic H5N1 AIV

    doi: 10.3390/ijms21072353

    Figure Lengend Snippet: Distribution of avian influenza virus NP in selected organs of inoculated birds. Distribution of influenza NP in brain ( A , E , I , M ), lung ( B , F , J , N ), spleen ( C , G , K , O ) and heart ( D , H , L , P ) of inoculated chickens of selected viruses at 4 dpi (except for H5N1_H4_T 327 K) as detected by immunohistochemistry using primary polyclonal rabbit anti-NP A/FPV/Rostock/34 antibody (1:750) and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200). 3-amino-9-ethyl-carbazol (red-brown); hematoxylin counterstain (blue); Nomarski contrast; bars A,B,D,E,F,H,I,J,L,M,N,P = 20 µm. Bars C,G,K,O = 50 µm.

    Article Snippet: Following sections were used for immunohistochemistry using the avidin–biotin–peroxidase complex method (Vector Laboratories Burlingame, CA, USA) with a primary polyclonal rabbit anti-NP antibody (1:750), and a secondary biotinylated goat anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) antibody (1:200) as described [ , ].

    Techniques: Immunohistochemistry, Plasmid Preparation

    DDI of capture antibodies from goat directed against human IgG (GAH). Immobilization efficiencies of three alternative techniques are compared: biotinylated GAH was immobilized by either direct physisorption (triangles), biotin–STV interaction (rectangles) or DDI (circles). Serial dilutions of the target antigen (human IgG, hIgG) were incubated in the wells containing the capture antibody. Signal detection was carried out by either regular ELISA ( A ), using an anti-hIgG–STV–alkaline phosphatase conjugate and fluorescence detection or IPCR ( B ) using an anti-human IgG-STV-DNA conjugate and real-time TaqMan detection. The standard deviation of duplicate measurements was

    Journal: Nucleic Acids Research

    Article Title: Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA-protein conjugates

    doi:

    Figure Lengend Snippet: DDI of capture antibodies from goat directed against human IgG (GAH). Immobilization efficiencies of three alternative techniques are compared: biotinylated GAH was immobilized by either direct physisorption (triangles), biotin–STV interaction (rectangles) or DDI (circles). Serial dilutions of the target antigen (human IgG, hIgG) were incubated in the wells containing the capture antibody. Signal detection was carried out by either regular ELISA ( A ), using an anti-hIgG–STV–alkaline phosphatase conjugate and fluorescence detection or IPCR ( B ) using an anti-human IgG-STV-DNA conjugate and real-time TaqMan detection. The standard deviation of duplicate measurements was

    Article Snippet: Preconjugates of HA24 and biotinylated antibodies were prepared by mixing 0.01 mM stock solutions of HA24 and equimolar amounts (0.01 mM stock solution) of biotinylated goat anti-rabbit IgG (Sigma), biotinylated goat anti-human IgG (Biotrend) or biotinylated rabbit anti-CEA (Dako) [biotinylated with NHS-Biotin (Pierce) according to the manufacturer’s instructions] in buffer A (10 mM Tris buffer, pH 7.5, containing 5 mM EDTA).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Fluorescence, Standard Deviation

    Flow cytometric analysis of cell surface expression of IL-18R. ( a ) Th1 (Dorris) and Th2 (D10) were stained with rabbit anti–IL-18R or preimmune serum (1/25 dilution) followed by biotinylated goat anti–rabbit IgG and were developed with PerCP-streptavidin. Similar results were obtained with X4 (Th1) and X12 (Th2) (not shown). ( b ) CD4 + T cells from OVA-TCR-αβ transgenic mice (D011.10) were driven to Th1 or Th2 lines for 6 d with APCs and antigen in the presence of IL-12 and anti–IL-4 antibody (Th1 line) or IL-4 (Th2 line). They were stained for cell surface IL-18R (with PerCP), and intracellular IFN-γ (with FITC) and IL-4 (with PE). All cells in b were activated with PMA/ionomycin for 4 h and Brefeldin A added in the last 2 h. Unfilled histograms , Staining with control preimmune serum. Similar results were obtained with cells driven for up to five rounds of culture.

    Journal: The Journal of Experimental Medicine

    Article Title: Selective Expression and Functions of Interleukin 18 Receptor on T Helper (Th) Type 1 but not Th2 Cells

    doi:

    Figure Lengend Snippet: Flow cytometric analysis of cell surface expression of IL-18R. ( a ) Th1 (Dorris) and Th2 (D10) were stained with rabbit anti–IL-18R or preimmune serum (1/25 dilution) followed by biotinylated goat anti–rabbit IgG and were developed with PerCP-streptavidin. Similar results were obtained with X4 (Th1) and X12 (Th2) (not shown). ( b ) CD4 + T cells from OVA-TCR-αβ transgenic mice (D011.10) were driven to Th1 or Th2 lines for 6 d with APCs and antigen in the presence of IL-12 and anti–IL-4 antibody (Th1 line) or IL-4 (Th2 line). They were stained for cell surface IL-18R (with PerCP), and intracellular IFN-γ (with FITC) and IL-4 (with PE). All cells in b were activated with PMA/ionomycin for 4 h and Brefeldin A added in the last 2 h. Unfilled histograms , Staining with control preimmune serum. Similar results were obtained with cells driven for up to five rounds of culture.

    Article Snippet: Cells were incubated with anti– IL-18R or normal rabbit serum followed by biotinylated goat anti–rabbit IgG (DAKO Corp., Carpinteria, CA) and then developed with PercP-streptavidin ( Becton Dickinson , Mountain View, CA).

    Techniques: Flow Cytometry, Expressing, Staining, Transgenic Assay, Mouse Assay

    Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with biotinylated anti-mouse IgG mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.

    Journal: Human Vaccines & Immunotherapeutics

    Article Title: A dendritic cell-targeted chimeric hepatitis B virus immunotherapeutic vaccine induces both cellular and humoral immune responses in vivo

    doi: 10.1080/21645515.2019.1689081

    Figure Lengend Snippet: Comparison of the binding of C-HBV to human (hPBMCs) with sheep (sPBMCS) peripheral blood mononuclear cells. PBMCs were isolated from either sheep or human blood and incubated for 1 h at 4°C with C-HBV (1–50 μg/mL). Following labeling with biotinylated anti-mouse IgG mAb and SA-PE-Cy5, bound protein was quantified by flow cytometry and expressed as the relative mean fluorescence intensity (MFI) of labeled cells.

    Article Snippet: Following overnight incubation at 37°C, cells were lysed with distilled water and captured IFN-γ visualized with rabbit anti-bovine IFN-γ antisera (Rockland Immunochemicals, 201-401-C41) and biotinylated-goat anti-rabbit IgG (H + L) (Thermo Fisher Scientific, 31820).

    Techniques: Binding Assay, Isolation, Incubation, Labeling, Flow Cytometry, Fluorescence