biotinylated anti-rat immunoglobulin Search Results


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  • 98
    Vector Laboratories biotinylated antirat immunoglobulin g antibody
    Biotinylated Antirat Immunoglobulin G Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies biotinylated anti rat immunoglobulin
    Biotinylated Anti Rat Immunoglobulin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare biotinylated sheep anti rat immunoglobulin
    Biotinylated Sheep Anti Rat Immunoglobulin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies biotinylated goat anti rat immunoglobulins
    Biotinylated Goat Anti Rat Immunoglobulins, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies biotinylated rabbit anti rat immunoglobulins
    Biotinylated Rabbit Anti Rat Immunoglobulins, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Abcam biotinylated goat anti rat immunoglobulin
    Biotinylated Goat Anti Rat Immunoglobulin, supplied by Abcam, used in various techniques. Bioz Stars score: 89/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare biotinylated goat anti rat immunoglobulin
    Biotinylated Goat Anti Rat Immunoglobulin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher biotinylated goat anti rat immunoglobulin
    Biotinylated Goat Anti Rat Immunoglobulin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson biotinylated goat anti rat immunoglobulin
    Biotinylated Goat Anti Rat Immunoglobulin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BioLegend biotinylated anti rat immunoglobulin igg antibody
    Biotinylated Anti Rat Immunoglobulin Igg Antibody, supplied by BioLegend, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies biotinylated polyclonal rabbit anti rat immunoglobulin
    Biotinylated Polyclonal Rabbit Anti Rat Immunoglobulin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SouthernBiotech biotinylated rat anti mouse immunoglobulin ig
    Biotinylated Rat Anti Mouse Immunoglobulin Ig, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotinylated sheep anti rat immunoglobulins g
    Biotinylated Sheep Anti Rat Immunoglobulins G, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare biotinylated anti rat immunoglobulin
    Biotinylated Anti Rat Immunoglobulin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotinylated anti rat immunoglobulin g igg
    mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of <t>immunoglobulin</t> G <t>(IgG1)</t> + GL-7 + and <t>IgG2a</t> + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p
    Biotinylated Anti Rat Immunoglobulin G Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Pharmingen biotinylated polyclonal anti rat immunoglobulin
    mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of <t>immunoglobulin</t> G <t>(IgG1)</t> + GL-7 + and <t>IgG2a</t> + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p
    Biotinylated Polyclonal Anti Rat Immunoglobulin, supplied by Pharmingen, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotinylated anti rat immunoglobulin g antibody
    mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of <t>immunoglobulin</t> G <t>(IgG1)</t> + GL-7 + and <t>IgG2a</t> + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p
    Biotinylated Anti Rat Immunoglobulin G Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson biotinylated anti rat immunoglobulin g2a
    mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of <t>immunoglobulin</t> G <t>(IgG1)</t> + GL-7 + and <t>IgG2a</t> + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p
    Biotinylated Anti Rat Immunoglobulin G2a, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Vector Laboratories non biotinylated rabbit anti rat immunoglobulin antibodies
    mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of <t>immunoglobulin</t> G <t>(IgG1)</t> + GL-7 + and <t>IgG2a</t> + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p
    Non Biotinylated Rabbit Anti Rat Immunoglobulin Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Jackson Immuno biotinylated mouse anti rat immunoglobulin
    mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of <t>immunoglobulin</t> G <t>(IgG1)</t> + GL-7 + and <t>IgG2a</t> + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p
    Biotinylated Mouse Anti Rat Immunoglobulin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of immunoglobulin G (IgG1) + GL-7 + and IgG2a + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p

    Journal: Frontiers in Immunology

    Article Title: The Kinase Complex mTOR Complex 2 Promotes the Follicular Migration and Functional Maturation of Differentiated Follicular Helper CD4+ T Cells During Viral Infection

    doi: 10.3389/fimmu.2018.01127

    Figure Lengend Snippet: mTOR complex 2 plays an essential role in regulating the migratory ability and effector function of follicular helper CD4 + T (T FH ) cells. (A) Rictor −/− or WT SMARTA T FH cells were incubated with LPS-activated B cells pulsed with cognate gp66-peptide or unpulsed (left). The frequency of T cell–B cell conjugates among total SMARTA T FH cells was quantified by flow cytometry (right) ( n = 3 samples per group). (B) Working model of the chemotaxis transwell assay for T FH cells (left). The absolute number of migrated T FH cells was quantified by flow cytometry, and the net migration efficiency (% of input) was calculated (right) ( n = 3 samples per group). (C) Experimental setup of the in vivo T FH function assay. (D) Representative flow cytometry results of the in vivo T FH function assay (top), Sap −/− mice receiving exogenous T FH cells from Rictor −/− (left) and WT mice (middle) or negative control without cell transfer (right) (top). Summary of frequency and total number of germinal center B cell and exogenous transferred T FH cells in Sap −/− host mice (bottom) ( n = 3 mice per group). (E) In vitro T FH function assay assessed at day 4 after culture. Flow cytometry of immunoglobulin G (IgG1) + GL-7 + and IgG2a + GL-7 + GC-like B cells cultured with Rictor −/− or WT T FH cells or in the absence of T FH cells (left). Quantification of the frequencies and total numbers of IgG1 + GL-7 + , IgG2a + GL-7 + GC-like B cells and T FH cells in culture (right) ( n = 4 samples per group). ns, not significant, * p

    Article Snippet: CXCR5 staining was performed using purified anti-CXCR5 (BD Biosciences) for 1 h at 4°C, followed by biotinylated anti-rat immunoglobulin G (IgG) (Jackson Immunoresearch) and then fluorescently labeled streptavidin (eBioscience) for 30 min on ice.

    Techniques: Incubation, Flow Cytometry, Cytometry, Chemotaxis Assay, Transwell Assay, Migration, In Vivo, Functional Assay, Mouse Assay, Negative Control, In Vitro, Cell Culture

    Impaired follicular helper CD4 + T (T FH ) differentiation leads to repressed humoral immunity in Rictor −/− mice. (A) Representative flow cytometry plots showing germinal center (GC) B (peanut agglutinin hi FAS hi ) cells in Rictor −/− versus WT mice at day 8, 10, and 15 after lymphocytic choriomeningitis virus (LCMV) infection (top). Summary of GC B cell frequency and cell number (bottom) ( n = 5–8 mice per group). (B) Confocal microscopy analysis of GC histology in spleen sections from Rictor −/− and WT mice at day 8 after LCMV infection (green: IgD, blue: GL-7, red: CD4) (Scale bar: 100 µm) (left). Quantification of GC size (right) ( n = 3 mice per group). (C) Titers of LCMV-specific immunoglobulin G in serum from Rictor −/− and WT mice at day 8, 15, 32, and 60 after LCMV infection were detected by enzyme-linked immunosorbent assay ( n = 5–7 mice per group). (D) Numbers of LCMV-specific antibody-secreting cells (ASCs) in bone marrow from Rictor −/− and WT mice at day 60 after infection, calculated by the enzyme-linked immunospot assay ( n = 5 mice per group). ns, not significant, * p

    Journal: Frontiers in Immunology

    Article Title: The Kinase Complex mTOR Complex 2 Promotes the Follicular Migration and Functional Maturation of Differentiated Follicular Helper CD4+ T Cells During Viral Infection

    doi: 10.3389/fimmu.2018.01127

    Figure Lengend Snippet: Impaired follicular helper CD4 + T (T FH ) differentiation leads to repressed humoral immunity in Rictor −/− mice. (A) Representative flow cytometry plots showing germinal center (GC) B (peanut agglutinin hi FAS hi ) cells in Rictor −/− versus WT mice at day 8, 10, and 15 after lymphocytic choriomeningitis virus (LCMV) infection (top). Summary of GC B cell frequency and cell number (bottom) ( n = 5–8 mice per group). (B) Confocal microscopy analysis of GC histology in spleen sections from Rictor −/− and WT mice at day 8 after LCMV infection (green: IgD, blue: GL-7, red: CD4) (Scale bar: 100 µm) (left). Quantification of GC size (right) ( n = 3 mice per group). (C) Titers of LCMV-specific immunoglobulin G in serum from Rictor −/− and WT mice at day 8, 15, 32, and 60 after LCMV infection were detected by enzyme-linked immunosorbent assay ( n = 5–7 mice per group). (D) Numbers of LCMV-specific antibody-secreting cells (ASCs) in bone marrow from Rictor −/− and WT mice at day 60 after infection, calculated by the enzyme-linked immunospot assay ( n = 5 mice per group). ns, not significant, * p

    Article Snippet: CXCR5 staining was performed using purified anti-CXCR5 (BD Biosciences) for 1 h at 4°C, followed by biotinylated anti-rat immunoglobulin G (IgG) (Jackson Immunoresearch) and then fluorescently labeled streptavidin (eBioscience) for 30 min on ice.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Infection, Confocal Microscopy, Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot