biotinylated anti-rabbit Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Vector Laboratories biotinylated anti rabbit antibody
    Increased d4EGFP expression in a mouse model of Alzheimer’s disease Representative images of DG hippocampus (coronal sections) from one month-old Tg Arc/Arg3.1-d4EGFP and Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP littermate mice are shown after visualization of d4EGFP by DAB (A, B) or immunofluorescence (C,D). Note the increased number of and labeling intensity of d4EGFP-positive neurons in CA1 (B) and DG granule cells (B, D) in the Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice. Brain sections stained with anti-GFP antibody and visualized by <t>biotinylated</t> (DAB method) or FITC-conjugated secondary antibodies. The fluorescent images are single confocal scans. Abbreviations: db – dorsal blade of DG, vb – ventral blade of DG; scale bars = 250 μm (A,B) and 100 μm (C,D). (E) Count of d4EGFP-positive neurons in Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice, as percent change from control Tg Arc/Arg3.1-d4EGFP littermates, in the DG: (% mean ± SD): 216.3 ± 7.7 vs. 100 ± 6.4 (**p
    Biotinylated Anti Rabbit Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1291 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit antibody/product/Vector Laboratories
    Average 99 stars, based on 1291 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit antibody - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    Agilent technologies biotinylated anti rabbit secondary antibodies
    Characterization of ERX-11 interactions with ER. ( A ) Nuclear lysates from E2-stimulated MCF-7 cells treated with vehicle or ERX-11 were subjected to immunoprecipitation with ER antibody. The immunoprecipates were analyzed by mass spectroscopy. The blue box represents the 222 proteins bound to ER in MCF-7 cells, whereas the orange box shows the 91 proteins whose binding to ER is disrupted by ERX-11 ( B ) Venn diagram shows the overlap between proteins binding with <t>biotinylated</t> ERX-11 in MCF-7 and ZR-75 cells. ( C ) Nuclear lysates from MDA-MB-231 cells treated with vehicle or biotin-ERX-11 were subject to immunoprecipitation using avidin beads. The avidin-biotin-ERX-11 precipitates were analyzed by mass spectroscopy. DOI: http://dx.doi.org/10.7554/eLife.26857.009
    Biotinylated Anti Rabbit Secondary Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit secondary antibodies/product/Agilent technologies
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit secondary antibodies - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated antirabbit antibody
    Characterization of ERX-11 interactions with ER. ( A ) Nuclear lysates from E2-stimulated MCF-7 cells treated with vehicle or ERX-11 were subjected to immunoprecipitation with ER antibody. The immunoprecipates were analyzed by mass spectroscopy. The blue box represents the 222 proteins bound to ER in MCF-7 cells, whereas the orange box shows the 91 proteins whose binding to ER is disrupted by ERX-11 ( B ) Venn diagram shows the overlap between proteins binding with <t>biotinylated</t> ERX-11 in MCF-7 and ZR-75 cells. ( C ) Nuclear lysates from MDA-MB-231 cells treated with vehicle or biotin-ERX-11 were subject to immunoprecipitation using avidin beads. The avidin-biotin-ERX-11 precipitates were analyzed by mass spectroscopy. DOI: http://dx.doi.org/10.7554/eLife.26857.009
    Biotinylated Antirabbit Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antirabbit antibody/product/Vector Laboratories
    Average 99 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    biotinylated antirabbit antibody - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    Jackson Immuno biotinylated secondary anti rabbit antibody
    The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of <t>biotinylated</t> NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .
    Biotinylated Secondary Anti Rabbit Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated secondary anti rabbit antibody/product/Jackson Immuno
    Average 90 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    biotinylated secondary anti rabbit antibody - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    94
    Abcam biotinylated anti rabbit
    The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of <t>biotinylated</t> NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .
    Biotinylated Anti Rabbit, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit/product/Abcam
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    92
    Biogenex biotinylated anti rabbit
    The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of <t>biotinylated</t> NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .
    Biotinylated Anti Rabbit, supplied by Biogenex, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit/product/Biogenex
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    GE Healthcare biotinylated anti rabbit
    The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of <t>biotinylated</t> NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .
    Biotinylated Anti Rabbit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit/product/GE Healthcare
    Average 92 stars, based on 92 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    89
    Agilent technologies biotinylated rabbit anti mouse antibody
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Rabbit Anti Mouse Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rabbit anti mouse antibody/product/Agilent technologies
    Average 89 stars, based on 269 article reviews
    Price from $9.99 to $1999.99
    biotinylated rabbit anti mouse antibody - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    92
    Thermo Fisher biotinylated anti rabbit
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Anti Rabbit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit/product/Thermo Fisher
    Average 92 stars, based on 153 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Agilent technologies biotinylated swine anti rabbit ab
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Swine Anti Rabbit Ab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated swine anti rabbit ab/product/Agilent technologies
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    biotinylated swine anti rabbit ab - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated goat antirabbit
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Goat Antirabbit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated goat antirabbit/product/Vector Laboratories
    Average 99 stars, based on 357 article reviews
    Price from $9.99 to $1999.99
    biotinylated goat antirabbit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    PerkinElmer biotinylated anti rabbit
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Anti Rabbit, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit/product/PerkinElmer
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    97
    Vector Laboratories biotinylated horse antirabbit igg
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Horse Antirabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated horse antirabbit igg/product/Vector Laboratories
    Average 97 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotinylated horse antirabbit igg - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology biotinylated anti rabbit
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Anti Rabbit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit/product/Santa Cruz Biotechnology
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    The Jackson Laboratory biotinylated anti rabbit
    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) <t>Biotinylated</t> mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.
    Biotinylated Anti Rabbit, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit/product/The Jackson Laboratory
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    Agilent technologies biotinylated rabbit anti goat antibody
    Expression of IL-17 receptors in DRG neurones in WT mice. ( a ) Representative agarose gel images of PCR products of IL-17 receptor subtypes in dorsal root ganglion (DRG) neurones of naïve WT mice. In WT mice a strong expression of IL-17RA, IL-17RC and IL-17RD and a weak expression of IL-17RB and IL-17RE is detectable. ( b ) Typical labelling of IL-17 receptors in DRG sections from naïve WT mice with anti-IL-17RA, -IL-17RB, -IL-17RC, -IL-17RD and anti-IL17RE antibodies. Arrows indicate representative cells considered positively labelled. Insets display labelling of sections at higher magnification and show that all the receptor subtypes were localised in subsets of neurones of different sizes (asterisks indicate all cells considered positively labelled) whereas IL-17RA and IL-17RC were also localised in satellite cells (exemplary cells marked by red triangles). Control staining only with rabbit-anti-goat <t>biotinylated</t> antibody (RAG) and goat-anti-rabbit biotinylated antibody (GAR). Magnification 200x (bars 15 µm), insets 400x. Values are mean ± SEM. IL-17R IL-17 receptor, H 2 0, water control, bp base pairs.
    Biotinylated Rabbit Anti Goat Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated rabbit anti goat antibody/product/Agilent technologies
    Average 90 stars, based on 107 article reviews
    Price from $9.99 to $1999.99
    biotinylated rabbit anti goat antibody - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated anti rabbit secondary ab
    Expression of IL-17 receptors in DRG neurones in WT mice. ( a ) Representative agarose gel images of PCR products of IL-17 receptor subtypes in dorsal root ganglion (DRG) neurones of naïve WT mice. In WT mice a strong expression of IL-17RA, IL-17RC and IL-17RD and a weak expression of IL-17RB and IL-17RE is detectable. ( b ) Typical labelling of IL-17 receptors in DRG sections from naïve WT mice with anti-IL-17RA, -IL-17RB, -IL-17RC, -IL-17RD and anti-IL17RE antibodies. Arrows indicate representative cells considered positively labelled. Insets display labelling of sections at higher magnification and show that all the receptor subtypes were localised in subsets of neurones of different sizes (asterisks indicate all cells considered positively labelled) whereas IL-17RA and IL-17RC were also localised in satellite cells (exemplary cells marked by red triangles). Control staining only with rabbit-anti-goat <t>biotinylated</t> antibody (RAG) and goat-anti-rabbit biotinylated antibody (GAR). Magnification 200x (bars 15 µm), insets 400x. Values are mean ± SEM. IL-17R IL-17 receptor, H 2 0, water control, bp base pairs.
    Biotinylated Anti Rabbit Secondary Ab, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated anti rabbit secondary ab/product/Vector Laboratories
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    biotinylated anti rabbit secondary ab - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Increased d4EGFP expression in a mouse model of Alzheimer’s disease Representative images of DG hippocampus (coronal sections) from one month-old Tg Arc/Arg3.1-d4EGFP and Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP littermate mice are shown after visualization of d4EGFP by DAB (A, B) or immunofluorescence (C,D). Note the increased number of and labeling intensity of d4EGFP-positive neurons in CA1 (B) and DG granule cells (B, D) in the Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice. Brain sections stained with anti-GFP antibody and visualized by biotinylated (DAB method) or FITC-conjugated secondary antibodies. The fluorescent images are single confocal scans. Abbreviations: db – dorsal blade of DG, vb – ventral blade of DG; scale bars = 250 μm (A,B) and 100 μm (C,D). (E) Count of d4EGFP-positive neurons in Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice, as percent change from control Tg Arc/Arg3.1-d4EGFP littermates, in the DG: (% mean ± SD): 216.3 ± 7.7 vs. 100 ± 6.4 (**p

    Journal: Journal of neuroscience methods

    Article Title: Fluorescent Arc/Arg3.1 indicator mice: a versatile tool to study brain activity changes in vitro and in vivo

    doi: 10.1016/j.jneumeth.2009.07.015

    Figure Lengend Snippet: Increased d4EGFP expression in a mouse model of Alzheimer’s disease Representative images of DG hippocampus (coronal sections) from one month-old Tg Arc/Arg3.1-d4EGFP and Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP littermate mice are shown after visualization of d4EGFP by DAB (A, B) or immunofluorescence (C,D). Note the increased number of and labeling intensity of d4EGFP-positive neurons in CA1 (B) and DG granule cells (B, D) in the Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice. Brain sections stained with anti-GFP antibody and visualized by biotinylated (DAB method) or FITC-conjugated secondary antibodies. The fluorescent images are single confocal scans. Abbreviations: db – dorsal blade of DG, vb – ventral blade of DG; scale bars = 250 μm (A,B) and 100 μm (C,D). (E) Count of d4EGFP-positive neurons in Tg 5XFAD /Tg Arc/Arg3.1-d4EGFP mice, as percent change from control Tg Arc/Arg3.1-d4EGFP littermates, in the DG: (% mean ± SD): 216.3 ± 7.7 vs. 100 ± 6.4 (**p

    Article Snippet: Briefly, slices were blocked with 10% NGS containing 0.1% Triton X-100 antibody in PBS for 1 hr and reacted with either anti-Arc (Arg3.1 antiserum, provided by Dietmar Kuhl, dilution 1:1,500) or anti-GFP (Molecular Probes, Cat# , dilution 1:2,000) rabbit antibody in 1% NGS containing PBS overnight at 4 C. After reaction with biotinylated anti-rabbit antibody (Vector laboratory, dilution 1:200), immunoreactions were visualized with the ABC elite kit (Vector laboratory) and DAB development procedure.

    Techniques: Expressing, Mouse Assay, Immunofluorescence, Labeling, Staining

    Characterization of ERX-11 interactions with ER. ( A ) Nuclear lysates from E2-stimulated MCF-7 cells treated with vehicle or ERX-11 were subjected to immunoprecipitation with ER antibody. The immunoprecipates were analyzed by mass spectroscopy. The blue box represents the 222 proteins bound to ER in MCF-7 cells, whereas the orange box shows the 91 proteins whose binding to ER is disrupted by ERX-11 ( B ) Venn diagram shows the overlap between proteins binding with biotinylated ERX-11 in MCF-7 and ZR-75 cells. ( C ) Nuclear lysates from MDA-MB-231 cells treated with vehicle or biotin-ERX-11 were subject to immunoprecipitation using avidin beads. The avidin-biotin-ERX-11 precipitates were analyzed by mass spectroscopy. DOI: http://dx.doi.org/10.7554/eLife.26857.009

    Journal: eLife

    Article Title: Estrogen receptor coregulator binding modulators (ERXs) effectively target estrogen receptor positive human breast cancers

    doi: 10.7554/eLife.26857

    Figure Lengend Snippet: Characterization of ERX-11 interactions with ER. ( A ) Nuclear lysates from E2-stimulated MCF-7 cells treated with vehicle or ERX-11 were subjected to immunoprecipitation with ER antibody. The immunoprecipates were analyzed by mass spectroscopy. The blue box represents the 222 proteins bound to ER in MCF-7 cells, whereas the orange box shows the 91 proteins whose binding to ER is disrupted by ERX-11 ( B ) Venn diagram shows the overlap between proteins binding with biotinylated ERX-11 in MCF-7 and ZR-75 cells. ( C ) Nuclear lysates from MDA-MB-231 cells treated with vehicle or biotin-ERX-11 were subject to immunoprecipitation using avidin beads. The avidin-biotin-ERX-11 precipitates were analyzed by mass spectroscopy. DOI: http://dx.doi.org/10.7554/eLife.26857.009

    Article Snippet: Biotinylated anti-rabbit secondary antibodies (DAKO Carpentaria, CA) were incubated for 60 min at room temperature after slides were washed for 1 hr in PBS.

    Techniques: Immunoprecipitation, Mass Spectrometry, Binding Assay, Multiple Displacement Amplification, Avidin-Biotin Assay

    Derivation of the α-helix mimetic ERX-11 and synthesis of tris-benzamide peptidomimetics. ( A ) Synthesis of 3-alkoxy-4-nitrobenzoic acids. ( B ) Solid-phase synthesis of tris-benzamdies (Reagents and conditions: ( a ) 3d , HATU, DIEA, DMF, rt, 24 hr; ( b ) SnCl 2 ⋅2H 2 O, AcOH/HCl/THF, rt, 24 hr; ( c ) 3 , HATU, DIEA, DMF, rt, 24 hr; ( d ) Piperidine for 7i ; ( e ) N,N-Di-Boc-1H-pyrazole-1-carboxamide, DIEA, DMF, rt, 12 hr for 7 j; ( f ) TFA/TIS/H 2 O (95:2.5:2.5), rt, 1 hr). ( c ) Synthesis of ERX-11 (Reagents and conditions: ( a ) HATU, DIEA, DMF, rt, 24 hr; ( b ) TFA, rt, 30 min) ( D ) Synthesis of biotinylated ERX-11. ( E ) Pull down of ER from Tamoxifen-resistant MCF-7 cells using biotinylated ERX-11 was not affected by the presence of Tamoxifen. DOI: http://dx.doi.org/10.7554/eLife.26857.006

    Journal: eLife

    Article Title: Estrogen receptor coregulator binding modulators (ERXs) effectively target estrogen receptor positive human breast cancers

    doi: 10.7554/eLife.26857

    Figure Lengend Snippet: Derivation of the α-helix mimetic ERX-11 and synthesis of tris-benzamide peptidomimetics. ( A ) Synthesis of 3-alkoxy-4-nitrobenzoic acids. ( B ) Solid-phase synthesis of tris-benzamdies (Reagents and conditions: ( a ) 3d , HATU, DIEA, DMF, rt, 24 hr; ( b ) SnCl 2 ⋅2H 2 O, AcOH/HCl/THF, rt, 24 hr; ( c ) 3 , HATU, DIEA, DMF, rt, 24 hr; ( d ) Piperidine for 7i ; ( e ) N,N-Di-Boc-1H-pyrazole-1-carboxamide, DIEA, DMF, rt, 12 hr for 7 j; ( f ) TFA/TIS/H 2 O (95:2.5:2.5), rt, 1 hr). ( c ) Synthesis of ERX-11 (Reagents and conditions: ( a ) HATU, DIEA, DMF, rt, 24 hr; ( b ) TFA, rt, 30 min) ( D ) Synthesis of biotinylated ERX-11. ( E ) Pull down of ER from Tamoxifen-resistant MCF-7 cells using biotinylated ERX-11 was not affected by the presence of Tamoxifen. DOI: http://dx.doi.org/10.7554/eLife.26857.006

    Article Snippet: Biotinylated anti-rabbit secondary antibodies (DAKO Carpentaria, CA) were incubated for 60 min at room temperature after slides were washed for 1 hr in PBS.

    Techniques:

    Immunohistochemical analysis of cannabinoid receptor expression in human normal skin and skin tumors. ( a ) Immunolocalization of CB 1 and CB 2 receptors. ( b ) Controls without primary Ab (only with secondary biotinylated anti-rabbit Ab), as well as controls with the Ab-blocking peptides, are shown (see Methods and Results for explanation). The source of samples is described in Methods. BCC, basal cell carcinoma; SCC, squamous cell carcinoma; Hf, hair follicle.

    Journal: Journal of Clinical Investigation

    Article Title: Inhibition of skin tumor growth and angiogenesis in vivo by activation of cannabinoid receptors

    doi: 10.1172/JCI200316116

    Figure Lengend Snippet: Immunohistochemical analysis of cannabinoid receptor expression in human normal skin and skin tumors. ( a ) Immunolocalization of CB 1 and CB 2 receptors. ( b ) Controls without primary Ab (only with secondary biotinylated anti-rabbit Ab), as well as controls with the Ab-blocking peptides, are shown (see Methods and Results for explanation). The source of samples is described in Methods. BCC, basal cell carcinoma; SCC, squamous cell carcinoma; Hf, hair follicle.

    Article Snippet: Sections were incubated with a biotinylated anti-rabbit (CB1 and CB2 ) or anti-rat Ab (CD31) and then with peroxidase-conjugated streptavidin (LSAB Kit Peroxidase; DAKO A/S, Glostrup, Denmark).

    Techniques: Immunohistochemistry, Expressing, Blocking Assay

    The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of biotinylated NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .

    Journal: Immunity

    Article Title: Particulate array of well-ordered HIV clade C Env trimers elicits neutralizing antibodies that display a unique V2 cap approach

    doi: 10.1016/j.immuni.2017.04.021

    Figure Lengend Snippet: The vaccine-induced autologous neutralizing mAbs target a V2 epitope (A) Neutralization potency (IC 50 , μg/ml) of the NHP mAbs with selected N-glycan modifications of 16055 Env compared to wt. (B) Plasma neutralization titers (ID 50 ) from each animal against wt 16055 compared to N301 N-glycan deleted virus. (C) Binding of biotinylated NHP mAbs to 16055 NFL TD CC trimers (His-captured) in the presence of non-biotinylated mAb competitors (left column) as assessed by ELISA, where 0% competition was the absorbance measured with no competitor present. ( D ) Structure of the V1 (cyan) V2 (green) V3 (orange) region from the clade G X1193.c1 trimer crystal structure (PDB 5FYJ, top left) with strands A, B, C/C′, D and loops V2a and V2b indicated; orientation within one protomer of the trimer shown below. Bar diagram schematic indicating locations of the variable region deletions in 16055 gp120 to map binding (top right) with relative binding to each deletion mutant compared to wt gp120 shown below; +, binding; –, no binding. ( E .

    Article Snippet: Secondary antibodies were: biotinylated anti-mouse (Dako), biotinylated anti-goat or biotinylated anti-rabbit (Jackson ImmunoResearch).

    Techniques: Neutralization, Binding Assay, Enzyme-linked Immunosorbent Assay, Mutagenesis

    Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) Biotinylated mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.

    Journal: Journal of Clinical Investigation

    Article Title: Resolution of psoriasis upon blockade of IL-15 biological activity in a xenograft mouse model

    doi: 10.1172/JCI200318986

    Figure Lengend Snippet: Recognition of receptor-bound IL-15 by mAb 146B7 and inhibition of IL-15–induced effects. ( a ) Biotinylated mAb 146B7 (filled squares) showed dose-dependent binding to IL-15 bound to IL-15Rα, which was coated onto an ELISA plate, whereas biotinylated 404E4 (filled triangles) did not show binding. Polyclonal huIgG1 (open squares) served as a control. This experiment was repeated two times, yielding similar results. ( b ) Dose-dependent binding of biotinylated mAb 146B7 (filled squares) to IL-15 bound to Raji lymphoma cells expressing IL-15Rα was shown by flow cytometry. Polyclonal huIgG1 (open squares) was used as a control. This experiment was repeated five times, yielding similar results. MFI, mean fluorescence intensity. ( c ) Effect of mAb 146B7 on IL-15–induced proliferation. Human PBMCs were incubated with IL-15 (12.5 ng/ml) in combination with mAb 146B7 (filled squares), mAb 404E4 (filled inverted triangles), or with huIgG1 (open squares) for 72 hours. BrdU incorporation was measured to assay proliferation. Representative data of 9 individual experiments are shown. ( d ) mAb 146B7 inhibits IL-15– but not IL-2–induced TNF-α production. Human PBMCs were incubated with IL-15 (0, 25, 100 ng/ml) or with IL-2 (100 ng/ml) in combination with mAb 146B7 at various concentrations for 72 hours. The amounts of TNF-α produced were measured by ELISA. A representative experiment from a series of 11 experiments is shown.

    Article Snippet: The other sections were incubated with 10% (vol/vol) normal rabbit serum (DAKO) in TBS for 10 minutes and stained according to the ABC method using the following primary antibodies: MIB-1 (Ki-67; Immunotech, Marseille, France), NCL-NK1 (CD57, Novocastra Laboratories Ltd., Newcastle, United Kingdom), PG-M1 (CD68; DAKO), or NCL-CD4-368 (CD4; Novocastra Laboratories), all diluted 1:50, then biotinylated rabbit anti-mouse antibody (DAKO) diluted 1:300 and streptABComplex/alkaline phosphatase (DAKO).

    Techniques: Inhibition, Binding Assay, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Cytometry, Fluorescence, Incubation, BrdU Incorporation Assay, Produced

    Expression of IL-17 receptors in DRG neurones in WT mice. ( a ) Representative agarose gel images of PCR products of IL-17 receptor subtypes in dorsal root ganglion (DRG) neurones of naïve WT mice. In WT mice a strong expression of IL-17RA, IL-17RC and IL-17RD and a weak expression of IL-17RB and IL-17RE is detectable. ( b ) Typical labelling of IL-17 receptors in DRG sections from naïve WT mice with anti-IL-17RA, -IL-17RB, -IL-17RC, -IL-17RD and anti-IL17RE antibodies. Arrows indicate representative cells considered positively labelled. Insets display labelling of sections at higher magnification and show that all the receptor subtypes were localised in subsets of neurones of different sizes (asterisks indicate all cells considered positively labelled) whereas IL-17RA and IL-17RC were also localised in satellite cells (exemplary cells marked by red triangles). Control staining only with rabbit-anti-goat biotinylated antibody (RAG) and goat-anti-rabbit biotinylated antibody (GAR). Magnification 200x (bars 15 µm), insets 400x. Values are mean ± SEM. IL-17R IL-17 receptor, H 2 0, water control, bp base pairs.

    Journal: Scientific Reports

    Article Title: Interleukin-17A is involved in mechanical hyperalgesia but not in the severity of murine antigen-induced arthritis

    doi: 10.1038/s41598-017-10509-5

    Figure Lengend Snippet: Expression of IL-17 receptors in DRG neurones in WT mice. ( a ) Representative agarose gel images of PCR products of IL-17 receptor subtypes in dorsal root ganglion (DRG) neurones of naïve WT mice. In WT mice a strong expression of IL-17RA, IL-17RC and IL-17RD and a weak expression of IL-17RB and IL-17RE is detectable. ( b ) Typical labelling of IL-17 receptors in DRG sections from naïve WT mice with anti-IL-17RA, -IL-17RB, -IL-17RC, -IL-17RD and anti-IL17RE antibodies. Arrows indicate representative cells considered positively labelled. Insets display labelling of sections at higher magnification and show that all the receptor subtypes were localised in subsets of neurones of different sizes (asterisks indicate all cells considered positively labelled) whereas IL-17RA and IL-17RC were also localised in satellite cells (exemplary cells marked by red triangles). Control staining only with rabbit-anti-goat biotinylated antibody (RAG) and goat-anti-rabbit biotinylated antibody (GAR). Magnification 200x (bars 15 µm), insets 400x. Values are mean ± SEM. IL-17R IL-17 receptor, H 2 0, water control, bp base pairs.

    Article Snippet: After incubation the PBS-washed sections were incubated for 2 hours with a biotinylated goat anti-rabbit antibody or a biotinylated rabbit anti-goat antibody (1:200; Dako) at 20 °C.

    Techniques: Expressing, Mouse Assay, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Staining