biotinylated anti-mouse igg Search Results


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  • 99
    Vector Laboratories biotinylated secondary antibodies
    Biotinylated Secondary Antibodies, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 11586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher biotinylated goat anti mouse igg
    Biotinylated Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated anti mouse igg
    Biotinylated Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 3885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated horse anti mouse igg
    Biotinylated Horse Anti Mouse Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 3018 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated antibody
    Biotinylated Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1886 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies biotinylated goat anti mouse igg
    Biotinylated Goat Anti Mouse Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies biotinylated anti mouse igg
    Biotinylated Anti Mouse Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson biotinylated rat anti mouse igg2a
    Analysis of rabbit peripheral blood mononuclear cells by multi-colour flow cytometry . PBMC were isolated from K 3 EDTA-sampled blood of naive rabbits before five-colour flow cytometry analysis. Specific detection of monocytes, B cells and T cell subsets consisted in a 3-step staining procedure. PBMC were first stained with anti-rabbit IgM (or mouse IgG1 isotype control), anti-rabbit CD8 (or mouse IgG1 isotype control) and anti-rabbit CD4 (or mouse <t>IgG2a</t> isotype control). Stainings were revealed with PE-conjugated rat anti-IgG1 or <t>biotinylated</t> rat anti-IgG2a, as secondary staining. Final staining was performed with streptavidin-APC, FITC-conjugated anti-rabbit T lymphocytes (or FITC-conjugated mouse IgG1 isotype control) and anti-human CD14 (or Pacific Blue-conjugated mouse IgG2a isotype control). Live lymphocytes were gated on 7-AAD - cells.
    Biotinylated Rat Anti Mouse Igg2a, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies biotinylated rabbit anti mouse igg
    Analysis of rabbit peripheral blood mononuclear cells by multi-colour flow cytometry . PBMC were isolated from K 3 EDTA-sampled blood of naive rabbits before five-colour flow cytometry analysis. Specific detection of monocytes, B cells and T cell subsets consisted in a 3-step staining procedure. PBMC were first stained with anti-rabbit IgM (or mouse IgG1 isotype control), anti-rabbit CD8 (or mouse IgG1 isotype control) and anti-rabbit CD4 (or mouse <t>IgG2a</t> isotype control). Stainings were revealed with PE-conjugated rat anti-IgG1 or <t>biotinylated</t> rat anti-IgG2a, as secondary staining. Final staining was performed with streptavidin-APC, FITC-conjugated anti-rabbit T lymphocytes (or FITC-conjugated mouse IgG1 isotype control) and anti-human CD14 (or Pacific Blue-conjugated mouse IgG2a isotype control). Live lymphocytes were gated on 7-AAD - cells.
    Biotinylated Rabbit Anti Mouse Igg, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Becton Dickinson biotinylated anti mouse igg1
    MOG-specific autoantibodies bind MOG-expressing cells and activate complement. (A) Binding of anti-MOG Ig from indicated mice to correctly conformed MOG on the cell surface of transduced EL4 cells (EL4-MOG) shown by flow cytometry. EL4 cells (top) and MOG-expressing EL4-MOG cells (bottom) were incubated with 1/200 diluted sera obtained from the indicated mice or 0.5 µg/ml 8.18-C5 mAb. Bound antibodies were detected by FACS using <t>biotinylated</t> <t>anti–IgG1-specific</t> antibody (allotype unspecific) and streptavidin-PE (SA-PE). A representative plot of three independent experiments is shown. (B) Complement activating capability of MOG binding antibodies in sera from transgenic mice. EL4 and EL4-MOG cells were incubated with sera (1/20 and 1/200 diluted) obtained from the indicated mice, each with three to five per group. Complement activating capability was measured by cell lysis using propidium iodide (PI) staining after incubation of sera-bound EL4 cells with rabbit complement. Background values were subtracted, and shown are mean values with the SEM of one experiment representative of three similar experiments.
    Biotinylated Anti Mouse Igg1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Analysis of rabbit peripheral blood mononuclear cells by multi-colour flow cytometry . PBMC were isolated from K 3 EDTA-sampled blood of naive rabbits before five-colour flow cytometry analysis. Specific detection of monocytes, B cells and T cell subsets consisted in a 3-step staining procedure. PBMC were first stained with anti-rabbit IgM (or mouse IgG1 isotype control), anti-rabbit CD8 (or mouse IgG1 isotype control) and anti-rabbit CD4 (or mouse IgG2a isotype control). Stainings were revealed with PE-conjugated rat anti-IgG1 or biotinylated rat anti-IgG2a, as secondary staining. Final staining was performed with streptavidin-APC, FITC-conjugated anti-rabbit T lymphocytes (or FITC-conjugated mouse IgG1 isotype control) and anti-human CD14 (or Pacific Blue-conjugated mouse IgG2a isotype control). Live lymphocytes were gated on 7-AAD - cells.

    Journal: Veterinary Research

    Article Title: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues

    doi: 10.1186/1297-9716-42-95

    Figure Lengend Snippet: Analysis of rabbit peripheral blood mononuclear cells by multi-colour flow cytometry . PBMC were isolated from K 3 EDTA-sampled blood of naive rabbits before five-colour flow cytometry analysis. Specific detection of monocytes, B cells and T cell subsets consisted in a 3-step staining procedure. PBMC were first stained with anti-rabbit IgM (or mouse IgG1 isotype control), anti-rabbit CD8 (or mouse IgG1 isotype control) and anti-rabbit CD4 (or mouse IgG2a isotype control). Stainings were revealed with PE-conjugated rat anti-IgG1 or biotinylated rat anti-IgG2a, as secondary staining. Final staining was performed with streptavidin-APC, FITC-conjugated anti-rabbit T lymphocytes (or FITC-conjugated mouse IgG1 isotype control) and anti-human CD14 (or Pacific Blue-conjugated mouse IgG2a isotype control). Live lymphocytes were gated on 7-AAD - cells.

    Article Snippet: After washing, cells were incubated for 10 min on ice with isotype-specific PE-conjugated rat anti-mouse IgG1 (A85-1, BD Biosciences, Erembodegem, Belgium) and biotinylated rat anti-mouse IgG2a (R19-15, BD Biosciences) antibodies.

    Techniques: Flow Cytometry, Cytometry, Isolation, Staining

    MOG-specific autoantibodies bind MOG-expressing cells and activate complement. (A) Binding of anti-MOG Ig from indicated mice to correctly conformed MOG on the cell surface of transduced EL4 cells (EL4-MOG) shown by flow cytometry. EL4 cells (top) and MOG-expressing EL4-MOG cells (bottom) were incubated with 1/200 diluted sera obtained from the indicated mice or 0.5 µg/ml 8.18-C5 mAb. Bound antibodies were detected by FACS using biotinylated anti–IgG1-specific antibody (allotype unspecific) and streptavidin-PE (SA-PE). A representative plot of three independent experiments is shown. (B) Complement activating capability of MOG binding antibodies in sera from transgenic mice. EL4 and EL4-MOG cells were incubated with sera (1/20 and 1/200 diluted) obtained from the indicated mice, each with three to five per group. Complement activating capability was measured by cell lysis using propidium iodide (PI) staining after incubation of sera-bound EL4 cells with rabbit complement. Background values were subtracted, and shown are mean values with the SEM of one experiment representative of three similar experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: Spontaneous relapsing-remitting EAE in the SJL/J mouse: MOG-reactive transgenic T cells recruit endogenous MOG-specific B cells

    doi: 10.1084/jem.20090299

    Figure Lengend Snippet: MOG-specific autoantibodies bind MOG-expressing cells and activate complement. (A) Binding of anti-MOG Ig from indicated mice to correctly conformed MOG on the cell surface of transduced EL4 cells (EL4-MOG) shown by flow cytometry. EL4 cells (top) and MOG-expressing EL4-MOG cells (bottom) were incubated with 1/200 diluted sera obtained from the indicated mice or 0.5 µg/ml 8.18-C5 mAb. Bound antibodies were detected by FACS using biotinylated anti–IgG1-specific antibody (allotype unspecific) and streptavidin-PE (SA-PE). A representative plot of three independent experiments is shown. (B) Complement activating capability of MOG binding antibodies in sera from transgenic mice. EL4 and EL4-MOG cells were incubated with sera (1/20 and 1/200 diluted) obtained from the indicated mice, each with three to five per group. Complement activating capability was measured by cell lysis using propidium iodide (PI) staining after incubation of sera-bound EL4 cells with rabbit complement. Background values were subtracted, and shown are mean values with the SEM of one experiment representative of three similar experiments.

    Article Snippet: EL4 and MOG-transduced EL4 cells (EL4-MOG; 2 × 105 /well) were incubated with sera at the indicated dilutions for 30–45 min at 4°C, washed intensively, and thereafter either stained with biotinylated anti–mouse IgG1 (BD) and streptavidin–PE diluted at 1/150 and PI at 1 µg/ml or incubated with LOW-TOX-M rabbit complement (Cedarlane Laboratories) at a 1/10 dilution for 90 min at 37°C and thereafter analyzed for lysis by staining with PI.

    Techniques: Expressing, Binding Assay, Mouse Assay, Flow Cytometry, Cytometry, Incubation, FACS, Transgenic Assay, Lysis, Staining