biotin-sp-affinipure goat anti-mouse Search Results


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  • 85
    Jackson Immuno biotin sp affinipure mouse anti goat igg
    Biotin Sp Affinipure Mouse Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure fab fragment goat anti mouse igg
    Biotin Sp Affinipure Fab Fragment Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg fcγ subclass 1 specific
    Biotin Sp Affinipure Goat Anti Mouse Igg Fcγ Subclass 1 Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg
    Biotin Sp Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 595 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg igm
    Biotin Sp Affinipure Goat Anti Mouse Igg Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg f ab 2 fragment specific
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure donkey anti goat igg
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg fcγ subclass 2c specific
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg Fcγ Subclass 2c Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti mouse igg
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg light chain specific
    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg Light Chain Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg fcγ fragment specific
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure Goat Anti Mouse Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti mouse igg igm
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Mouse Igg Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure f ab 2 fragment goat anti mouse igg fcγ fragment specific
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure F Ab 2 Fragment Goat Anti Mouse Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure f
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno immunolabeling
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Immunolabeling, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg subclasses 1 2a 2b 3 fcγ fragment specific
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure Goat Anti Mouse Igg Subclasses 1 2a 2b 3 Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Immunofluorescence, Staining, Cell Culture

    IgG2c is the major IgG subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a biotinylated goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.

    Journal: Mechanisms of ageing and development

    Article Title: Obesity induces pro-inflammatory B cells and impairs B cell function in old mice

    doi: 10.1016/j.mad.2017.01.004

    Figure Lengend Snippet: IgG2c is the major IgG subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a biotinylated goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.

    Article Snippet: Detection antibody was a biotinylated goat anti-mouse IgG antibody (Jackson ImmunoResearch Labs 115-065-071), followed by streptavidin-HRP.

    Techniques: Enzyme-linked Immunosorbent Assay, Purification, Concentration Assay, Spectrophotometry, Staining, Fluorescence