biotin-sp-affinipure goat Jackson Immuno Search Results


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  • 99
    Jackson Immuno biotin sp affinipure goat anti horseradish peroxidase
    Biotin Sp Affinipure Goat Anti Horseradish Peroxidase, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure donkey anti goat igg
    Biotin Sp Affinipure Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg
    Biotin Sp Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti human igg
    Biotin Sp Affinipure Goat Anti Human Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure rabbit anti goat igg
    Biotin Sp Affinipure Rabbit Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti rat igg
    Biotin Sp Affinipure Goat Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure mouse anti goat igg
    Biotin Sp Affinipure Mouse Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure bovine anti goat igg
    Biotin Sp Affinipure Bovine Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti guinea pig igg
    Biotin Sp Affinipure Goat Anti Guinea Pig Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti armenian hamster igg
    Biotin Sp Affinipure Goat Anti Armenian Hamster Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin conjugated goat antimouse igg f
    Biotin Conjugated Goat Antimouse Igg F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp conjugated goat antimouse igg igm
    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total <t>IgM</t> (A) and <t>IgG</t> (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.
    Biotin Sp Conjugated Goat Antimouse Igg Igm, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti chicken igy igg
    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total <t>IgM</t> (A) and <t>IgG</t> (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.
    Biotin Sp Affinipure Goat Anti Chicken Igy Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 98/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti rabbit igg
    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total <t>IgM</t> (A) and <t>IgG</t> (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.
    Biotin Sp Affinipure Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp conjugate affinipure goat anti mouse antibody
    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total <t>IgM</t> (A) and <t>IgG</t> (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.
    Biotin Sp Conjugate Affinipure Goat Anti Mouse Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti human igg fcγ fragment specific
    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, <t>biotinylated</t> <t>anti-IgG</t> antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Biotin Sp Affinipure Goat Anti Human Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti cat igg fc fragment specific
    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, <t>biotinylated</t> <t>anti-IgG</t> antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Biotin Sp Affinipure Goat Anti Cat Igg Fc Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure fab fragment goat anti rat igg
    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, <t>biotinylated</t> <t>anti-IgG</t> antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Biotin Sp Affinipure Fab Fragment Goat Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure fab fragment goat anti mouse igg
    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, <t>biotinylated</t> <t>anti-IgG</t> antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Biotin Sp Affinipure Fab Fragment Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno anti rabbit biotin sp conjugated affinipure goat secondary antibody
    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, <t>biotinylated</t> <t>anti-IgG</t> antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.
    Anti Rabbit Biotin Sp Conjugated Affinipure Goat Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg fcγ fragment specific
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure Goat Anti Mouse Igg Fcγ Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno goat anti mouse igg biotin
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Goat Anti Mouse Igg Biotin, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure fab fragment goat anti human igg
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure Fab Fragment Goat Anti Human Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure f
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure F, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure f ab 2 fragment rabbit anti goat igg
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure F Ab 2 Fragment Rabbit Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
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    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure Fab Fragment Donkey Anti Goat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno biotin sp affinipure goat anti mouse igg light chain specific
    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Affinipure Goat Anti Mouse Igg Light Chain Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
    Biotin Sp Conjugated Affinipure Goat Anti Human Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IgG2c is the major <t>IgG</t> subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a <t>biotinylated</t> goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.
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    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
    Biotin Sp Affinipure Goat Anti Mouse Igg F Ab 2 Fragment Specific, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated <t>AffiniPure</t> goat anti-mouse <t>IgG,F(ab′)2</t> fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p
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    Image Search Results


    DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Journal: PLoS ONE

    Article Title: Tumor-Derived Autophagosomes (DRibbles) Induce B Cell Activation in a TLR2-MyD88 Dependent Manner

    doi: 10.1371/journal.pone.0053564

    Figure Lengend Snippet: DRibbles derived from tumor cells induce specific antibody production in vivo. (A and B) Serum was collected from C57/BL6 mice at day 7 after first intravenous injection of PBS or Hep1-6 derived DRibbles (DRs). The total IgM (A) and IgG (B) in serum was measured by ELISA. Values are the mean ± SEM derived from three mice per group (n = 3). (C and D) Hep1-6 or B16F10 cells were incubated for 1 hour with serum derived from PBS or Hep1-6 DRibbles injected mice respectively. After washing, Hep1-6 or B16F10 cells were incubated with FITC-labeled anti-mouse IgM antibody (C) or FITC-labeled anti-mouse IgG antibody (D). After washing, the cells were analyzed by flow cytometry. (E and F) HepI-6 or B16F10 cells were treated as C and D, and then the cells were stained with DAPI and analyzed by fluorescence microscope. Green fluorescence represents FITC-labeled anti-mouse IgM antibody (E) or FITC-labeled anti-mouse IgG antibody (F). Blue fluorescence represents the cell nucleus. A representative of three independent experiments was showed.

    Article Snippet: Briefly, The plates were coated with either goat anti-mouse IgG or IgM (Jackson Immuno Research Laboratories) at 1∶10000 overnight at 4°C and washed with ELISA washing buffer (PBS plus 0.5% Tween-20).

    Techniques: Derivative Assay, In Vivo, Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Incubation, Labeling, Flow Cytometry, Cytometry, Staining, Fluorescence, Microscopy

    GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Journal: Scientific Reports

    Article Title: Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus

    doi: 10.1038/srep27623

    Figure Lengend Snippet: GMR biosensor autoantigen microarrays. ( a ) Optical images of a GMR biosensor chip and a cartridge with a reaction well (left). The sensor chip measures 10 × 12 mm and consists of an array of 8 × 10 sensors (total 80 sensors). Each sensor size is 100 × 100 μm (right). ( b ) A schematic of assaying antibody reactivity to autoantigens (not to scale). (1) Autoantigens were printed on the surface of the chip’s sensors. (2) The sample was added to the reaction well, allowing antibodies to bind to their corresponding antigens. (3) After washing, species-specific, biotinylated anti-IgG antibodies were used as a secondary reagent. (4) Streptavidin-coated MNPs bind to the biotinylated detection antibodies, and the respective sensor detects stray field from the bound MNPs.

    Article Snippet: The microarrays were then washed with rinsing buffer and incubated for 1 hour with biotinylated anti-human IgG (109-065-098, Jackson ImmunoResearch, PA, USA; at 100 ng/mL), anti-mouse IgG (ab98711, Abcam, MA, USA; at 50 ng/mL), or anti-rabbit IgG (ab97198, Abcam; 100 ng/mL) secondary antibodies.

    Techniques: Chromatin Immunoprecipitation

    IgG2c is the major IgG subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a biotinylated goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.

    Journal: Mechanisms of ageing and development

    Article Title: Obesity induces pro-inflammatory B cells and impairs B cell function in old mice

    doi: 10.1016/j.mad.2017.01.004

    Figure Lengend Snippet: IgG2c is the major IgG subclass in the VAT. A. IgG subclasses in protein lysates of VAT lymphocytes were measured by Ig ELISA, after coating the plates with goat anti-mouse Southern Biotech purified anti-subclass antibodies at the concentration of 2 μg/ml. Detection antibody was a biotinylated goat anti-mouse IgG antibody, followed by streptavidin-HRP. Results are ratios of OD in subclasses/OD in total IgG. OD were measured with a spectrophotometer at 450 nm. B-C. ic staining of IgG2c in VAT ABC (B) and in splenic ABC (C) was performed after 48 h in culture in the absence (shadow, dotted line) or presence (solid line) of CpG. Mean Fluorescence Intensity from 3 independent experiments ± SE are shown in each quadrant. D-E. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and ABC percentages in VAT and spleen. Pearson's r = 0.85, p = 0.03 and r = 0.97, p = 0.002, respectively. F-G. Correlation between IgG2c (ratios of OD in IgG2c/OD in total IgG) in VAT lysates and FO percentages in VAT and spleen. Pearson's r = 0.09, p = 0.86 and r = −0.7, p = 0.13, respectively.

    Article Snippet: Detection antibody was a biotinylated goat anti-mouse IgG antibody (Jackson ImmunoResearch Labs 115-065-071), followed by streptavidin-HRP.

    Techniques: Enzyme-linked Immunosorbent Assay, Purification, Concentration Assay, Spectrophotometry, Staining, Fluorescence

    Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: T cell antigen discovery using soluble vaccinia proteome reveals recognition of antigens with both virion and non-virion association *

    doi: 10.4049/jimmunol.1400663

    Figure Lengend Snippet: Immunogenicity of purified VACV-WR antigen, measured by antibody production and protection against VACV-WR challenge in mice (A) IgG subtype analysis. Antibodies were engendered in C57Bl/6 mice against nickel column-purified VACV IMV membrane protein, WR101/H3LΔTM that has been adjuvanted in CpG/ISCOMs or alum, or in PBS alone as a control. Sera were obtained after 14 days and probed against VACV proteome microarrays on to which 8 two-fold serial dilutions of purified WR101/H3L were printed. Specific reactivity to purified WR101/H3L was visualized using fluorescently-tagged secondary antibodies to IgG, IgG1 and IgG2c and signal intensities quantified in a confocal laser scanner; data for a single concentration of printed antigen is shown. (B) Relative proportions of IgG1 and IgG2c derived from data shown in (A). The IgG2a proportion of the total signal is shown above the zero line, and the IgG1 proportion shown below. The IgG response is polarized according to adjuvant. (C) Protection of B6 mice against intranasal (i.n.) challenge of VACV-WR using adjuvanted WR101/H3LΔTM and WR101/H3L. CpG/ISCOMs reduce weight loss and promote recovery compared to alum or PBS. (D) and (E) correlations between nadir body weight (expressed as percentage of original body weight) and titer of IgG2c and IgG1, respectively. Titer was defined from the WR101/H3L titration series on the array at the lowest concentration to give a signal intensity > 2000. Liner regression was used to generate the trend lines.

    Article Snippet: Biotinylated goat anti-mouse IgG, IgG1 and IgG2c secondary antibodies were purchased from Jackson ImmunoResearch (Cat# 115-065-008, 115-065-205, and 115-065-208, respectively).

    Techniques: Purification, Mouse Assay, Nickel Column, Concentration Assay, Derivative Assay, Titration

    Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Acquisition of anti-CD19 chimeric antigen receptors (CARs) by NK cells from donor cells via trogocytosis. A. Flow cytometry dot plots illustrating the uptake of anti-CD19 CARs by NK cells via trogocytosis. NK cells co-cultured with K562 cells (control) or K562-antiCD19BBζ cells were stained with an anti-CD56-FITC antibody and a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by PE-conjugated streptavidin. B. Uptake of anti-CD19 CARs by NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells. The data are presented as the mean ± SD of values obtained using three unrelated NK donors. C. Kinetics of anti-CD19 CAR uptake by NK cells from K562-antiCD19BBζ cells (black bars) and control K562 cells (white bars). The uptake of anti-CD19 CARs by NK cells was analyzed after co-culturing with feeder cells for the indicated time and was compared with that of NK cells co-cultured with control K562 cells. The data are presented as the mean ± SD of values obtained using 3 unrelated NK cell donors. *: significant increase compared with control cells (p

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Flow Cytometry, Cytometry, Cell Culture, Staining, Irradiation

    Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunofluorescence analysis for trogocytosis. A. NK cells stained with anti-CD56-FITC antibody. B. K562-antiCD19BBζ cells were stained with a biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment specific antibody, followed by Alexa Fluor 568-conjugated streptavidin. The nucleus was stained with DAPI (blue). C. NK cells co-cultured with K562-antiCD19BBζ cells were stained for CD56 and anti-CD19 CARs, as previously described. D. Acquisition of anti-CD19-BB-ζ by NK cells via trogocytosis was observed.

    Article Snippet: Biotin-SP-conjugated AffiniPure goat anti-mouse IgG,F(ab′)2 fragment-specific antibody (Jackson ImmunoResearch 115-065-072) and PE-conjugated streptavidin (Jackson ImmunoResearch 016-110-084) were used for labeling the cells.

    Techniques: Immunofluorescence, Staining, Cell Culture