biotin-peg3 - Search Results


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  • 85
    Millipore ez link phosphine peg3 biotin
    Ez Link Phosphine Peg3 Biotin, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher amine peg3 biotin
    Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore azide peg3 biotin conjugate
    Azide Peg3 Biotin Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher ez link psoralen peg3 biotin
    Ez Link Psoralen Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    ChemPep Inc biotin peg3 propionic acid
    Biotin Peg3 Propionic Acid, supplied by ChemPep Inc, used in various techniques. Bioz Stars score: 87/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher ez link psoralen peg3 biotin bpso
    Ez Link Psoralen Peg3 Biotin Bpso, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Jena Bioscience azide peg3 biotin conjugate
    Azide Peg3 Biotin Conjugate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 83/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher ez link tfpa peg3 biotin kit
    Ez Link Tfpa Peg3 Biotin Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Click Chemistry Tools dbco s s peg3 biotin
    Dbco S S Peg3 Biotin, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher ez link amine peg3 biotin
    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with <t>amine-PEG3-biotin</t> (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Ez Link Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Creative PEGWorks heterobifunctional biotin peg3 4kd amine linker
    Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and <t>triphenylphosphine-PEG3.4kD</t> -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).
    Heterobifunctional Biotin Peg3 4kd Amine Linker, supplied by Creative PEGWorks, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Journal: Nucleic Acids Research

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

    doi: 10.1093/nar/gkw741

    Figure Lengend Snippet: Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Article Snippet: Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min.

    Techniques: Magnetic Beads, Isolation, Incubation, Immunoprecipitation

    Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and triphenylphosphine-PEG3.4kD -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).

    Journal:

    Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin

    doi: 10.1016/j.actbio.2010.01.027

    Figure Lengend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (A) Upon reaction between rTM-N3 and triphenylphosphine-PEG3.4kD -biotin, SDS PAGE reveals the presence of two species separated by approximately 4 kD (Lane 1), corresponding to the desired biotinylated conjugate (*) and unreacted rTM-N3 . A molecular weight shift was not observed in a parallel control reaction using rTM engineered without an azido group (Lane 2), demonstrating the specificity of the Staudinger ligation. (B) Western blot against human TM after initial conjugation (Lane 1) and subsequent purification (Lane 2). After purification via centrifugal dialysis and monomeric avidin chromatography, a single species corresponding to the expected molecular weight of the desired biotin-PEG-TM conjugate is observed (*). (C) Western blot against biotin using HRP-labeled streptavidin confirms biotinylation of the construct (*; Lane 1).

    Article Snippet: A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ].

    Techniques: Recombinant, SDS Page, Molecular Weight, Ligation, Western Blot, Conjugation Assay, Purification, Avidin-Biotin Assay, Chromatography, Labeling, Construct

    Site-specific biotinylation of recombinant human thrombomodulin (rTM) through Staudinger ligation rTM was engineered with a C-terminal azido group (1) and subsequently reacted with triphenylphosphine-PEG3.4kD -biotin (2) .

    Journal:

    Article Title: Biomolecular Surface Engineering of Pancreatic Islets with Thrombomodulin

    doi: 10.1016/j.actbio.2010.01.027

    Figure Lengend Snippet: Site-specific biotinylation of recombinant human thrombomodulin (rTM) through Staudinger ligation rTM was engineered with a C-terminal azido group (1) and subsequently reacted with triphenylphosphine-PEG3.4kD -biotin (2) .

    Article Snippet: A triphenylphosphine-poly(ethylene glycol)-biotin conjugate was synthesized by reaction of a heterobifunctional biotin-PEG3.4kD -amine linker (CreativePEGWorks, Winston Salem, NC) with a pentafluorophenyl (PFP) active ester of triphenylphosphine, synthesized as described previously [ , ].

    Techniques: Recombinant, Ligation