biotin rna labeling mix Roche Search Results


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  • 99
    Thermo Fisher biotin 14 datp
    Biotin 14 Datp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotin rna labeling mix
    Biotin Rna Labeling Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche biotin rna labeling mix
    Validation of human PUM mRNA targets. <t>RNA-protein</t> complexes formed between <t>biotinylated</t> 3′-UTRs and extracts of HeLa S3 cells expressing PUM1-HD-TAP and PUM2-HD-TAP were purified on streptavidin magnetic beads and monitored for the presence of TAP-PUM-HD by immunoblot analysis with anti-PAP antibody. (A) Biotin-labeled 3′-UTR sequences for indicated genes (lanes 3 to 8) were incubated with PUM1-HD-TAP and PUM2-HD-TAP extracts (lane 1). Rps26 3′-UTR was used as negative control probe RNA (lanes 8/9). The supernatant after pull-down with INTS2 is shown in lane 2. (B) Validation of the PUF-binding motif. Biotinylated RNA corresponding to the Dll1 3′-UTR was combined with PUM1-HD-TAP extract (lane 2) and 100-fold excess of competitor RNA (R1; AUUGUAAAUA; lane 3) or control RNA where the core motif is mutated (R2; AUACAAAAUA; lane 4). A fragment of MET 3′-UTR bearing wild type (UGU) or mutant (ACA) PUF binding sites is shown in lanes 5 and 6.
    Biotin Rna Labeling Mix, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1892 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher biotin rna labeling mix
    SRSF1 directly interacts with the VEGF <t>RNA</t> domain discriminating VEGF and VEGFxxxb isoforms ( A ) <t>REMSAs</t> were performed by incubating the labeled wild-type (WT) or by mutating VEGF RNA probe with increasing amount of purified GST-SRSF1 or GST in SRSF1 binding sites handling. Free probe was also shown as a control. ( B ) SRSF1 expression was tested in mock-transfected (Mock) (NT) or transfected ACHN, Caki-2 and 786-O cells with scramble or siRNA directed against SRSF1. Tubulin is shown as a loading control. ( C ) REMSAs were performed by incubating the labeled wild-type (WT) or mutating VEGF RNA probe with 1 mg of total extracts of mock in SRSF1 binding sites, si RNA (siC) or SRSF1 siRNA (siSR) transfected cells handling. Free probe alone and in the presence of 50 nM of GST or GST-SRSF1 were also shown as controls. Asterisks show the retarded bands which intensity was decreased when cells extracts from siSRSF1-transfected cells were used in comparison to the retarded bands observed with extracts from mock or siC transfected cells.
    Biotin Rna Labeling Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche biotin rna labelling mix
    SRSF1 directly interacts with the VEGF <t>RNA</t> domain discriminating VEGF and VEGFxxxb isoforms ( A ) <t>REMSAs</t> were performed by incubating the labeled wild-type (WT) or by mutating VEGF RNA probe with increasing amount of purified GST-SRSF1 or GST in SRSF1 binding sites handling. Free probe was also shown as a control. ( B ) SRSF1 expression was tested in mock-transfected (Mock) (NT) or transfected ACHN, Caki-2 and 786-O cells with scramble or siRNA directed against SRSF1. Tubulin is shown as a loading control. ( C ) REMSAs were performed by incubating the labeled wild-type (WT) or mutating VEGF RNA probe with 1 mg of total extracts of mock in SRSF1 binding sites, si RNA (siC) or SRSF1 siRNA (siSR) transfected cells handling. Free probe alone and in the presence of 50 nM of GST or GST-SRSF1 were also shown as controls. Asterisks show the retarded bands which intensity was decreased when cells extracts from siSRSF1-transfected cells were used in comparison to the retarded bands observed with extracts from mock or siC transfected cells.
    Biotin Rna Labelling Mix, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche biotin rna labeling mix sp6 t7
    SRSF1 directly interacts with the VEGF <t>RNA</t> domain discriminating VEGF and VEGFxxxb isoforms ( A ) <t>REMSAs</t> were performed by incubating the labeled wild-type (WT) or by mutating VEGF RNA probe with increasing amount of purified GST-SRSF1 or GST in SRSF1 binding sites handling. Free probe was also shown as a control. ( B ) SRSF1 expression was tested in mock-transfected (Mock) (NT) or transfected ACHN, Caki-2 and 786-O cells with scramble or siRNA directed against SRSF1. Tubulin is shown as a loading control. ( C ) REMSAs were performed by incubating the labeled wild-type (WT) or mutating VEGF RNA probe with 1 mg of total extracts of mock in SRSF1 binding sites, si RNA (siC) or SRSF1 siRNA (siSR) transfected cells handling. Free probe alone and in the presence of 50 nM of GST or GST-SRSF1 were also shown as controls. Asterisks show the retarded bands which intensity was decreased when cells extracts from siSRSF1-transfected cells were used in comparison to the retarded bands observed with extracts from mock or siC transfected cells.
    Biotin Rna Labeling Mix Sp6 T7, supplied by Roche, used in various techniques. Bioz Stars score: 83/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche biotinylated rna labeling mix
    EGFR-AS1 promotes the maintenance of EGFR mRNA stability by binding to HuR. a Silver staining SDS-PAGE gel of electrophoretically separated proteins immunoprecipitated with EGFR-AS1 and its antisense <t>RNA</t> in 786-O cells. b RNA pull-down assay was performed in 786-O and A498 cells using <t>biotinylated</t> EGFR-AS1 or antisense RNA probe transcribed in vitro and detected by western blots. c Upper: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR-AS1 RNA enrichment in immunoprecipitated complexes. IgG is the negative control. Lower: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR RNA enrichment in immunoprecipitated complexes. d RIP assay of the enrichment of EGFR mRNA with HuR between the EGFR-AS1 knockdown and NC group in RCC cells. IgG was used as an internal control. e Upper: RNA FISH analysis of EGFR mRNA (green) and immunofluorescence detection of HuR (red) in RCC cells. The rightmost graph shows colocalization between the green signal (EGFR) and the red signal (HuR). Pearson’s R = 0.583. Scale bar = 50 μm. Lower: RNA FISH analysis of EGFR-AS1 (green) and immunofluorescence detection of HuR (red) in RCC cells. Pearson’s R = 0.416. f The rate of degradation of the EGFR mRNA between the HuR knockdown and control group using RNA stability assays in RCC cells. g The rate of degradation of the EGFR mRNA between the HuR overexpressing and control group using RNA stability assays in RCC cells. h The rate of degradation of the EGFR mRNA in the EGFR-AS1 knockdown and control cells transfected with pcDNA3.1 + -HuR over 12 h in KETR-3 and ACHN cells. i The rate of degradation of the EGFR mRNA in the EGFR-AS1 overexpressing and control cells transfected with HuR siRNA over 12 h
    Biotinylated Rna Labeling Mix, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche dig rna labeling mix
    EGFR-AS1 promotes the maintenance of EGFR mRNA stability by binding to HuR. a Silver staining SDS-PAGE gel of electrophoretically separated proteins immunoprecipitated with EGFR-AS1 and its antisense <t>RNA</t> in 786-O cells. b RNA pull-down assay was performed in 786-O and A498 cells using <t>biotinylated</t> EGFR-AS1 or antisense RNA probe transcribed in vitro and detected by western blots. c Upper: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR-AS1 RNA enrichment in immunoprecipitated complexes. IgG is the negative control. Lower: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR RNA enrichment in immunoprecipitated complexes. d RIP assay of the enrichment of EGFR mRNA with HuR between the EGFR-AS1 knockdown and NC group in RCC cells. IgG was used as an internal control. e Upper: RNA FISH analysis of EGFR mRNA (green) and immunofluorescence detection of HuR (red) in RCC cells. The rightmost graph shows colocalization between the green signal (EGFR) and the red signal (HuR). Pearson’s R = 0.583. Scale bar = 50 μm. Lower: RNA FISH analysis of EGFR-AS1 (green) and immunofluorescence detection of HuR (red) in RCC cells. Pearson’s R = 0.416. f The rate of degradation of the EGFR mRNA between the HuR knockdown and control group using RNA stability assays in RCC cells. g The rate of degradation of the EGFR mRNA between the HuR overexpressing and control group using RNA stability assays in RCC cells. h The rate of degradation of the EGFR mRNA in the EGFR-AS1 knockdown and control cells transfected with pcDNA3.1 + -HuR over 12 h in KETR-3 and ACHN cells. i The rate of degradation of the EGFR mRNA in the EGFR-AS1 overexpressing and control cells transfected with HuR siRNA over 12 h
    Dig Rna Labeling Mix, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 3700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jena Bioscience biotin pcr labeling mix
    EGFR-AS1 promotes the maintenance of EGFR mRNA stability by binding to HuR. a Silver staining SDS-PAGE gel of electrophoretically separated proteins immunoprecipitated with EGFR-AS1 and its antisense <t>RNA</t> in 786-O cells. b RNA pull-down assay was performed in 786-O and A498 cells using <t>biotinylated</t> EGFR-AS1 or antisense RNA probe transcribed in vitro and detected by western blots. c Upper: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR-AS1 RNA enrichment in immunoprecipitated complexes. IgG is the negative control. Lower: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR RNA enrichment in immunoprecipitated complexes. d RIP assay of the enrichment of EGFR mRNA with HuR between the EGFR-AS1 knockdown and NC group in RCC cells. IgG was used as an internal control. e Upper: RNA FISH analysis of EGFR mRNA (green) and immunofluorescence detection of HuR (red) in RCC cells. The rightmost graph shows colocalization between the green signal (EGFR) and the red signal (HuR). Pearson’s R = 0.583. Scale bar = 50 μm. Lower: RNA FISH analysis of EGFR-AS1 (green) and immunofluorescence detection of HuR (red) in RCC cells. Pearson’s R = 0.416. f The rate of degradation of the EGFR mRNA between the HuR knockdown and control group using RNA stability assays in RCC cells. g The rate of degradation of the EGFR mRNA between the HuR overexpressing and control group using RNA stability assays in RCC cells. h The rate of degradation of the EGFR mRNA in the EGFR-AS1 knockdown and control cells transfected with pcDNA3.1 + -HuR over 12 h in KETR-3 and ACHN cells. i The rate of degradation of the EGFR mRNA in the EGFR-AS1 overexpressing and control cells transfected with HuR siRNA over 12 h
    Biotin Pcr Labeling Mix, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 70/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher biotin 14 ctp
    EGFR-AS1 promotes the maintenance of EGFR mRNA stability by binding to HuR. a Silver staining SDS-PAGE gel of electrophoretically separated proteins immunoprecipitated with EGFR-AS1 and its antisense <t>RNA</t> in 786-O cells. b RNA pull-down assay was performed in 786-O and A498 cells using <t>biotinylated</t> EGFR-AS1 or antisense RNA probe transcribed in vitro and detected by western blots. c Upper: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR-AS1 RNA enrichment in immunoprecipitated complexes. IgG is the negative control. Lower: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR RNA enrichment in immunoprecipitated complexes. d RIP assay of the enrichment of EGFR mRNA with HuR between the EGFR-AS1 knockdown and NC group in RCC cells. IgG was used as an internal control. e Upper: RNA FISH analysis of EGFR mRNA (green) and immunofluorescence detection of HuR (red) in RCC cells. The rightmost graph shows colocalization between the green signal (EGFR) and the red signal (HuR). Pearson’s R = 0.583. Scale bar = 50 μm. Lower: RNA FISH analysis of EGFR-AS1 (green) and immunofluorescence detection of HuR (red) in RCC cells. Pearson’s R = 0.416. f The rate of degradation of the EGFR mRNA between the HuR knockdown and control group using RNA stability assays in RCC cells. g The rate of degradation of the EGFR mRNA between the HuR overexpressing and control group using RNA stability assays in RCC cells. h The rate of degradation of the EGFR mRNA in the EGFR-AS1 knockdown and control cells transfected with pcDNA3.1 + -HuR over 12 h in KETR-3 and ACHN cells. i The rate of degradation of the EGFR mRNA in the EGFR-AS1 overexpressing and control cells transfected with HuR siRNA over 12 h
    Biotin 14 Ctp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Validation of human PUM mRNA targets. RNA-protein complexes formed between biotinylated 3′-UTRs and extracts of HeLa S3 cells expressing PUM1-HD-TAP and PUM2-HD-TAP were purified on streptavidin magnetic beads and monitored for the presence of TAP-PUM-HD by immunoblot analysis with anti-PAP antibody. (A) Biotin-labeled 3′-UTR sequences for indicated genes (lanes 3 to 8) were incubated with PUM1-HD-TAP and PUM2-HD-TAP extracts (lane 1). Rps26 3′-UTR was used as negative control probe RNA (lanes 8/9). The supernatant after pull-down with INTS2 is shown in lane 2. (B) Validation of the PUF-binding motif. Biotinylated RNA corresponding to the Dll1 3′-UTR was combined with PUM1-HD-TAP extract (lane 2) and 100-fold excess of competitor RNA (R1; AUUGUAAAUA; lane 3) or control RNA where the core motif is mutated (R2; AUACAAAAUA; lane 4). A fragment of MET 3′-UTR bearing wild type (UGU) or mutant (ACA) PUF binding sites is shown in lanes 5 and 6.

    Journal: PLoS ONE

    Article Title: Comparative Analysis of mRNA Targets for Human PUF-Family Proteins Suggests Extensive Interaction with the miRNA Regulatory System

    doi: 10.1371/journal.pone.0003164

    Figure Lengend Snippet: Validation of human PUM mRNA targets. RNA-protein complexes formed between biotinylated 3′-UTRs and extracts of HeLa S3 cells expressing PUM1-HD-TAP and PUM2-HD-TAP were purified on streptavidin magnetic beads and monitored for the presence of TAP-PUM-HD by immunoblot analysis with anti-PAP antibody. (A) Biotin-labeled 3′-UTR sequences for indicated genes (lanes 3 to 8) were incubated with PUM1-HD-TAP and PUM2-HD-TAP extracts (lane 1). Rps26 3′-UTR was used as negative control probe RNA (lanes 8/9). The supernatant after pull-down with INTS2 is shown in lane 2. (B) Validation of the PUF-binding motif. Biotinylated RNA corresponding to the Dll1 3′-UTR was combined with PUM1-HD-TAP extract (lane 2) and 100-fold excess of competitor RNA (R1; AUUGUAAAUA; lane 3) or control RNA where the core motif is mutated (R2; AUACAAAAUA; lane 4). A fragment of MET 3′-UTR bearing wild type (UGU) or mutant (ACA) PUF binding sites is shown in lanes 5 and 6.

    Article Snippet: Biotinylated RNAs were produced with T7-RNA polymerase with biotin RNA labeling mixture (Roche) as described .

    Techniques: Expressing, Purification, Magnetic Beads, Labeling, Incubation, Negative Control, Binding Assay, Mutagenesis

    SRSF1 directly interacts with the VEGF RNA domain discriminating VEGF and VEGFxxxb isoforms ( A ) REMSAs were performed by incubating the labeled wild-type (WT) or by mutating VEGF RNA probe with increasing amount of purified GST-SRSF1 or GST in SRSF1 binding sites handling. Free probe was also shown as a control. ( B ) SRSF1 expression was tested in mock-transfected (Mock) (NT) or transfected ACHN, Caki-2 and 786-O cells with scramble or siRNA directed against SRSF1. Tubulin is shown as a loading control. ( C ) REMSAs were performed by incubating the labeled wild-type (WT) or mutating VEGF RNA probe with 1 mg of total extracts of mock in SRSF1 binding sites, si RNA (siC) or SRSF1 siRNA (siSR) transfected cells handling. Free probe alone and in the presence of 50 nM of GST or GST-SRSF1 were also shown as controls. Asterisks show the retarded bands which intensity was decreased when cells extracts from siSRSF1-transfected cells were used in comparison to the retarded bands observed with extracts from mock or siC transfected cells.

    Journal: Oncotarget

    Article Title: Targeting the pro-angiogenic forms of VEGF or inhibiting their expression as anti-cancer strategies

    doi: 10.18632/oncotarget.13942

    Figure Lengend Snippet: SRSF1 directly interacts with the VEGF RNA domain discriminating VEGF and VEGFxxxb isoforms ( A ) REMSAs were performed by incubating the labeled wild-type (WT) or by mutating VEGF RNA probe with increasing amount of purified GST-SRSF1 or GST in SRSF1 binding sites handling. Free probe was also shown as a control. ( B ) SRSF1 expression was tested in mock-transfected (Mock) (NT) or transfected ACHN, Caki-2 and 786-O cells with scramble or siRNA directed against SRSF1. Tubulin is shown as a loading control. ( C ) REMSAs were performed by incubating the labeled wild-type (WT) or mutating VEGF RNA probe with 1 mg of total extracts of mock in SRSF1 binding sites, si RNA (siC) or SRSF1 siRNA (siSR) transfected cells handling. Free probe alone and in the presence of 50 nM of GST or GST-SRSF1 were also shown as controls. Asterisks show the retarded bands which intensity was decreased when cells extracts from siSRSF1-transfected cells were used in comparison to the retarded bands observed with extracts from mock or siC transfected cells.

    Article Snippet: RNA electromobility shift assays (REMSAs) For REMSA experiments, 30 pmol of biotinylated RNA using Biotin RNA Labeling Mix (Pierce Chemical) was combined with increasing concentrations (0, 0.1, 1, 5, 10, 50 nmol/L) of GST fusion proteins or 1 mg of cell extracts, in a previously described binding buffer [ ].

    Techniques: Labeling, Purification, Binding Assay, Expressing, Transfection

    EGFR-AS1 promotes the maintenance of EGFR mRNA stability by binding to HuR. a Silver staining SDS-PAGE gel of electrophoretically separated proteins immunoprecipitated with EGFR-AS1 and its antisense RNA in 786-O cells. b RNA pull-down assay was performed in 786-O and A498 cells using biotinylated EGFR-AS1 or antisense RNA probe transcribed in vitro and detected by western blots. c Upper: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR-AS1 RNA enrichment in immunoprecipitated complexes. IgG is the negative control. Lower: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR RNA enrichment in immunoprecipitated complexes. d RIP assay of the enrichment of EGFR mRNA with HuR between the EGFR-AS1 knockdown and NC group in RCC cells. IgG was used as an internal control. e Upper: RNA FISH analysis of EGFR mRNA (green) and immunofluorescence detection of HuR (red) in RCC cells. The rightmost graph shows colocalization between the green signal (EGFR) and the red signal (HuR). Pearson’s R = 0.583. Scale bar = 50 μm. Lower: RNA FISH analysis of EGFR-AS1 (green) and immunofluorescence detection of HuR (red) in RCC cells. Pearson’s R = 0.416. f The rate of degradation of the EGFR mRNA between the HuR knockdown and control group using RNA stability assays in RCC cells. g The rate of degradation of the EGFR mRNA between the HuR overexpressing and control group using RNA stability assays in RCC cells. h The rate of degradation of the EGFR mRNA in the EGFR-AS1 knockdown and control cells transfected with pcDNA3.1 + -HuR over 12 h in KETR-3 and ACHN cells. i The rate of degradation of the EGFR mRNA in the EGFR-AS1 overexpressing and control cells transfected with HuR siRNA over 12 h

    Journal: Cell Death & Disease

    Article Title: Long noncoding RNA EGFR-AS1 promotes cell growth and metastasis via affecting HuR mediated mRNA stability of EGFR in renal cancer

    doi: 10.1038/s41419-019-1331-9

    Figure Lengend Snippet: EGFR-AS1 promotes the maintenance of EGFR mRNA stability by binding to HuR. a Silver staining SDS-PAGE gel of electrophoretically separated proteins immunoprecipitated with EGFR-AS1 and its antisense RNA in 786-O cells. b RNA pull-down assay was performed in 786-O and A498 cells using biotinylated EGFR-AS1 or antisense RNA probe transcribed in vitro and detected by western blots. c Upper: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR-AS1 RNA enrichment in immunoprecipitated complexes. IgG is the negative control. Lower: RIP assays were performed in 786-O cells using HuR antibody to detect EGFR RNA enrichment in immunoprecipitated complexes. d RIP assay of the enrichment of EGFR mRNA with HuR between the EGFR-AS1 knockdown and NC group in RCC cells. IgG was used as an internal control. e Upper: RNA FISH analysis of EGFR mRNA (green) and immunofluorescence detection of HuR (red) in RCC cells. The rightmost graph shows colocalization between the green signal (EGFR) and the red signal (HuR). Pearson’s R = 0.583. Scale bar = 50 μm. Lower: RNA FISH analysis of EGFR-AS1 (green) and immunofluorescence detection of HuR (red) in RCC cells. Pearson’s R = 0.416. f The rate of degradation of the EGFR mRNA between the HuR knockdown and control group using RNA stability assays in RCC cells. g The rate of degradation of the EGFR mRNA between the HuR overexpressing and control group using RNA stability assays in RCC cells. h The rate of degradation of the EGFR mRNA in the EGFR-AS1 knockdown and control cells transfected with pcDNA3.1 + -HuR over 12 h in KETR-3 and ACHN cells. i The rate of degradation of the EGFR mRNA in the EGFR-AS1 overexpressing and control cells transfected with HuR siRNA over 12 h

    Article Snippet: RNA pull-down assay and mass spectrometry LncRNA EGFR-AS1 was transcribed in vitro from the vector pSPT19-lncRNA-EGFR-AS1 and biotinylated with biotinylated RNA labeling mix (Roche, Mannheim, Germany) and T7 RNA polymerase (Roche) and treated with RNase-free DNase I (Roche).

    Techniques: Binding Assay, Silver Staining, SDS Page, Immunoprecipitation, Pull Down Assay, In Vitro, Western Blot, Negative Control, Fluorescence In Situ Hybridization, Immunofluorescence, Transfection