Journal: Ecology and Evolution
Article Title: Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples, et al. Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples
Figure Lengend Snippet: DNA amplification rates in invertebrate ( n = 117) and vertebrate samples ( n = 53) extracted with CTAB protocol and the BioSprint ® platform together with the DNA Blood Kit using general primers (left) and prey‐specific primers (right); for all samples taken together and separately for invertebrates and vertebrates, respectively. Amplification success for plant samples was 100% for both extraction methods (data not shown). CTAB only represents the share of samples that tested positive when they were CTAB ‐extracted and negative when using BioSprint ® 96. For BioSprint only, this was exactly the other way round, that is, the share of samples that tested positive for BioSprint ® 96 and negative for CTAB . Both positive is the share of samples with successful PCR amplifications for both extraction methods. Asterisks indicate significant differences
Article Snippet: The automated DNA extraction was performed using the BioSprint® 96 DNA Blood Kit on a BioSprint® 96 extraction robotic platform (Qiagen).
Techniques: Amplification, Polymerase Chain Reaction