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  • 99
    Bio-Rad bioplex buffer
    <t>Bioplex</t> analysis of IL-8 release (pg/ml) in IB3-1 cells induced with TNF- α in the absence and in the presence of compound 1 ( 1 μ M); the effects of 1 μ M and 10 μ M TMA were included for comparison ( n = 3). IB3-1 cells were treated with compound 1 for 5 hours and then stimulated with TNF- α for further 24 hours. The supernatants were analyzed using a Bioplex cytokine assay.
    Bioplex Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioplex buffer/product/Bio-Rad
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bioplex buffer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    85
    Bio-Rad bioplex lysis buffer
    Activation of downstream pathways by mutant EGFR. Transgenic NR6 cells expressing wtEGFR, R324L and E330K were plated under serum free conditions and then lysed and tested by <t>Bioplex</t> for ( A ) pERK1/2 and ( B ) pAkt levels. Data is presented as mean fluorescent intensity ± S.E. or as a ratio of p-Akt to t-Akt ± S.E. of three independent replicates corrected for total Akt protein.
    Bioplex Lysis Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioplex lysis buffer/product/Bio-Rad
    Average 85 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    bioplex lysis buffer - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Bioplex analysis of IL-8 release (pg/ml) in IB3-1 cells induced with TNF- α in the absence and in the presence of compound 1 ( 1 μ M); the effects of 1 μ M and 10 μ M TMA were included for comparison ( n = 3). IB3-1 cells were treated with compound 1 for 5 hours and then stimulated with TNF- α for further 24 hours. The supernatants were analyzed using a Bioplex cytokine assay.

    Journal: Mediators of Inflammation

    Article Title: Differential Effects of Angelicin Analogues on NF-κB Activity and IL-8 Gene Expression in Cystic Fibrosis IB3-1 Cells

    doi: 10.1155/2017/2389487

    Figure Lengend Snippet: Bioplex analysis of IL-8 release (pg/ml) in IB3-1 cells induced with TNF- α in the absence and in the presence of compound 1 ( 1 μ M); the effects of 1 μ M and 10 μ M TMA were included for comparison ( n = 3). IB3-1 cells were treated with compound 1 for 5 hours and then stimulated with TNF- α for further 24 hours. The supernatants were analyzed using a Bioplex cytokine assay.

    Article Snippet: Finally, plates were washed by vacuum filtration three times, beads were suspended in Bioplex assay buffer, and samples were analyzed on a Bio-Rad 96-well plate reader using the Bioplex Suspension Array System and Bioplex Manager software (Bio-Rad Laboratories Inc., Hercules, CA).

    Techniques: Cytokine Assay

    Activation of downstream pathways by mutant EGFR. Transgenic NR6 cells expressing wtEGFR, R324L and E330K were plated under serum free conditions and then lysed and tested by Bioplex for ( A ) pERK1/2 and ( B ) pAkt levels. Data is presented as mean fluorescent intensity ± S.E. or as a ratio of p-Akt to t-Akt ± S.E. of three independent replicates corrected for total Akt protein.

    Journal: Cancers

    Article Title: Glioma Specific Extracellular Missense Mutations in the First Cysteine Rich Region of Epidermal Growth Factor Receptor (EGFR) Initiate Ligand Independent Activation

    doi: 10.3390/cancers3022032

    Figure Lengend Snippet: Activation of downstream pathways by mutant EGFR. Transgenic NR6 cells expressing wtEGFR, R324L and E330K were plated under serum free conditions and then lysed and tested by Bioplex for ( A ) pERK1/2 and ( B ) pAkt levels. Data is presented as mean fluorescent intensity ± S.E. or as a ratio of p-Akt to t-Akt ± S.E. of three independent replicates corrected for total Akt protein.

    Article Snippet: Whole cell lysates (WCL) were prepared using Bioplex cell lysis buffer (BioRad, Hercules, CA) and pAkt and pERK1/2 were quantified according to the manufacturer's instructions.

    Techniques: Activation Assay, Mutagenesis, Transgenic Assay, Expressing