Journal: BMC Genomics
Article Title: Whole transcriptomic analysis of the plant-beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 during enhanced biofilm formation regulated by maize root exudates
Figure Lengend Snippet: Gene expression profiles for all significant genes a and several representative categories. b Transporters. c Metabolism. d Cell motility and chemotaxis. e NRPS PKS. f Biofilm-formation related. g Plant growth-promotion related. For e-g , all important genes (both significantly and insignificantly differentially expressed) are included. The color bar in the heatmap figures indicates the ratio of expression level of each gene between the presence of root exudates and its absence. Each number represents the category of the significantly affected genes: 1, cell wall; 2, transporters; 3, sensors; 4, membrane bioenergetics; 5, motility and chemotaxis; 6, protein secretion; 7, cell division; 8, sporulation and germination; 9, metabolism of carbohydrates and related molecules; 10, metabolism of amino acids and related molecules; 11, metabolism of nucleotides and nucleic acids; 12, metabolism of lipids; 13, metabolism of coenzymes and prosthetic groups, phosphate, and sulfur; 14, DNA replication, restriction/modification, repair, recombination, packaging and segregation; 15, RNA synthesis; 16, RNA modification; 17, protein synthesis, modification and folding; 18, adaptation to atypical conditions; 19, detoxification; 20, antibiotic production; and 21, phage-related functions
Article Snippet: Considering the dynamics curves of biofilm formation, cells were collected and RNA extracted 24 and 48 h post-inoculation, which represented the mid-exponential phase (biomass quickly increasing) and stationary phase (biomass peaked and remained stable) during the biofilm formation, respectively (Additional file : Figure S1).
Techniques: Expressing, Chemotaxis Assay, Modification