Article Title: Prevalence, Molecular Typing, and Determination of the Biofilm-Forming Ability of Listeria monocytogenes Serotypes from Poultry Meat and Poultry Preparations in Spain
Figure Lengend Snippet: Three-dimensional projections of structures of biofilms obtained from one-micron optical sections on the z -axis acquired through confocal laser scanning microscopy. These images represent an overhead view of the biofilms formed by 20 strains of Listeria monocytogenes , with virtual projection of shadows to the right. Each square represented had length of side of 119 μm. Strains: 1 (serotype 1/2a), 2 (1/2a), 3 (1/2b), 4 (1/2b), 5 (1/2c), 6 (1/2c), 7 (3a), 8 (3b), 9 (3b), 10 (3c), 11 (3c), 12 (4a), 13 (4a), 14 (4b), 15 (4b), 16 (4b), 17 (4b), 18 (4c), 19 (4d), 20 (4d).
Article Snippet: Three-dimensional images of the biofilms were reconstructed by means of the IMARIS 9.1 program (Bitplane AG, Zurich, Switzerland) and quantitative structural parameters (biovolume, percentage surface coverage, roughness, maximum thickness) were calculated.
Techniques: Confocal Laser Scanning Microscopy