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  • 89
    Agilent technologies agilent technologies bioanalyzer
    Single and multiplex PCR amplification products for selected staphylococcal genes. Lanes: 1, mecA ; 2, aacA-aphD ; 3, tetK ; 4, tetM ; 5, erm (A); 6, erm (C); 7, vat (A); 8, vat (B); 9, vat (C); 10, 16S rRNA; 11, multiplex; M, molecular size standard. (A) DNA fragments were visualized on a 2.5% agarose gel and stained with ethidium bromide. (B) Fragment analysis was performed with the <t>Agilent</t> <t>Bioanalyzer</t> and the DNA 1000 LabChip kit.
    Agilent Technologies Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 319 article reviews
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    94
    Agilent technologies agilent technologies 2100 bioanalyzer
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologies 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 5209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent technologies 2100 bioanalyzer/product/Agilent technologies
    Average 94 stars, based on 5209 article reviews
    Price from $9.99 to $1999.99
    agilent technologies 2100 bioanalyzer - by Bioz Stars, 2020-07
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    91
    Agilent technologies agilent technologies 2001 bioanalyzer
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologies 2001 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent technologies 2001 bioanalyzer/product/Agilent technologies
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    agilent technologies 2001 bioanalyzer - by Bioz Stars, 2020-07
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    94
    Thermo Fisher agilent technologies 2100 bioanalyzer
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologies 2100 Bioanalyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent technologies 2100 bioanalyzer/product/Thermo Fisher
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    agilent technologies 2100 bioanalyzer - by Bioz Stars, 2020-07
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    85
    Agilent technologies agilent technologies bioanalyzer labchip
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologies Bioanalyzer Labchip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    agilent technologies bioanalyzer labchip - by Bioz Stars, 2020-07
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    90
    Agilent technologies agilent technologie 2100 bioanalyzer
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologie 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent technologie 2100 bioanalyzer/product/Agilent technologies
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    agilent technologie 2100 bioanalyzer - by Bioz Stars, 2020-07
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    94
    Agilent technologies agilent technologies 2100 bioanalyze
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologies 2100 Bioanalyze, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent technologies 2100 bioanalyze/product/Agilent technologies
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    88
    Agilent technologies agilent technologies 2100 bioanalyser
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologies 2100 Bioanalyser, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent technologies 2100 bioanalyser/product/Agilent technologies
    Average 88 stars, based on 140 article reviews
    Price from $9.99 to $1999.99
    agilent technologies 2100 bioanalyser - by Bioz Stars, 2020-07
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    86
    Agilent technologies agilent technologies bioanalyzer rna
    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an <t>Agilent</t> 2100 <t>Bioanalyzer.</t> Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.
    Agilent Technologies Bioanalyzer Rna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Single and multiplex PCR amplification products for selected staphylococcal genes. Lanes: 1, mecA ; 2, aacA-aphD ; 3, tetK ; 4, tetM ; 5, erm (A); 6, erm (C); 7, vat (A); 8, vat (B); 9, vat (C); 10, 16S rRNA; 11, multiplex; M, molecular size standard. (A) DNA fragments were visualized on a 2.5% agarose gel and stained with ethidium bromide. (B) Fragment analysis was performed with the Agilent Bioanalyzer and the DNA 1000 LabChip kit.

    Journal: Journal of Clinical Microbiology

    Article Title: Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in Staphylococcus aureus

    doi: 10.1128/JCM.41.9.4089-4094.2003

    Figure Lengend Snippet: Single and multiplex PCR amplification products for selected staphylococcal genes. Lanes: 1, mecA ; 2, aacA-aphD ; 3, tetK ; 4, tetM ; 5, erm (A); 6, erm (C); 7, vat (A); 8, vat (B); 9, vat (C); 10, 16S rRNA; 11, multiplex; M, molecular size standard. (A) DNA fragments were visualized on a 2.5% agarose gel and stained with ethidium bromide. (B) Fragment analysis was performed with the Agilent Bioanalyzer and the DNA 1000 LabChip kit.

    Article Snippet: Using the Agilent Bioanalyzer technology, we drastically reduced the time for analysis to a total of approximately 2.5 h. Further work will focus on the combination of the multiplex PCR assay with DNA microarray technology to (i) further accelerate the analysis of amplification products, (ii) enhance the sensitivity of amplimer detection, and (iii) include a specificity control by hybridization to gene-specific oligonucleotide capture probes.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Staining

    FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an Agilent 2100 Bioanalyzer. Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Fanconi anemia protein FANCI functions in ribosome biogenesis

    doi: 10.1073/pnas.1811557116

    Figure Lengend Snippet: FANCI functions in LSU pre-rRNA processing. ( A ). The 41S, 32S, and 12S pre-rRNAs are detected by a probe (P5) that hybridizes with ITS2. ( B ) FANCI depletion results in LSU pre-rRNA processing defects. HeLa cells were transfected with the indicated siRNAs, and total RNA was harvested after 72 h of depletion. Pre-rRNAs were separated by gel electrophoresis, and Northern blots were performed using radioactively labeled P5 probe. Northern blotting with a probe against the 7SL RNA was used as a loading control. Small illustrations of the pre-rRNAs are to the right of their respective bands. ( C – I ) profiles for Northern blotting from three biological replicates for mock ( C ), siFANCI-pool ( D ), siFANCI-2 ( E ), siFANCI-3 ( F ), siPES1 ( G ), siNOL11 ( H ), and siFANCD2 ( I ). Statistical significance was calculated using a two-way ANOVA with Sidak multiple comparisons test (mean ± SD). All comparisons are relative to siNT. The x axes of all graphs were equalized, except for siPES1 in G , which has larger RAMP values. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. Unmarked bars are not significant. ( J ) FANCI depletion results in decreased mature 28S rRNA relative to 18S. The ratio of 28S/18S was calculated using an Agilent 2100 Bioanalyzer. Statistical significance was calculated using a one-tailed unpaired Mann–Whitney U test for three biological replicates (mean ± SD). All comparisons are relative to siNT.

    Article Snippet: To measure the ratio of mature 28S to 18S rRNAs, total RNA that was prepared as described above was run on an Agilent Technologies 2100 Bioanalyzer at the Yale Center for Genome Analysis.

    Techniques: Transfection, Nucleic Acid Electrophoresis, Northern Blot, Labeling, One-tailed Test, MANN-WHITNEY

    Electropherogram and “gel-like” image of positive control on the Agilent Bioanalyzer 2100. The x -axis on the electropherogram represents amplicon size (bp), whist the y -axis represents the measurement response of fluorescence units (FUs). Amplicons of interest in order of size (bp) are lower marker (15), Zein (80), Lectin (90), CaMV (108) and combined CaMV 35s promoter/NOS terminator (131) amplicons, and the upper marker (1500).

    Journal: Biotechnology Research International

    Article Title: Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification

    doi: 10.4061/2011/838232

    Figure Lengend Snippet: Electropherogram and “gel-like” image of positive control on the Agilent Bioanalyzer 2100. The x -axis on the electropherogram represents amplicon size (bp), whist the y -axis represents the measurement response of fluorescence units (FUs). Amplicons of interest in order of size (bp) are lower marker (15), Zein (80), Lectin (90), CaMV (108) and combined CaMV 35s promoter/NOS terminator (131) amplicons, and the upper marker (1500).

    Article Snippet: Agilent Bioanalyzer 2100 Agilent Technologies state the Bioanalyzer 2100's advantages to be ease-of-use, speed of analysis, low sample volumes, and high reproducibility [ ].

    Techniques: Positive Control, Amplification, Fluorescence, Marker