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  • 95
    Agilent technologies 2100 bioanalyzer bioa
    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and <t>2100</t> <t>Bioanalyzer</t> electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.
    2100 Bioanalyzer Bioa, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 1057 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer bioa/product/Agilent technologies
    Average 95 stars, based on 1057 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer bioa - by Bioz Stars, 2020-04
    95/100 stars
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    86
    Schiff Nutrition International bioa
    UV–Vis spectroscopy of <t>PLP</t> or PLP-bound (holo) <t>BioA</t> under various conditions. ( a ) Spectra of 200 µ M holo BioA (purple), 200 µ M PLP in 100 m M Tris pH 7.6 (black), 200 µ M PLP in 100 m
    Bioa, supplied by Schiff Nutrition International, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioa/product/Schiff Nutrition International
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bioa - by Bioz Stars, 2020-04
    86/100 stars
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    94
    Agilent technologies agilent bioa nalyzer
    GW-COBRA and LA-COBRA library construction results. ( A ) Sonicated genomic DNA isolated from HCT116 cell line (lane 1), 50 ng of Adapter-2 ligated library material (lane 2). Column purified Adapter-2 ligated library material (lane 3); ( B ) PCR amplification test of bisulfite treated library materials. Lane 1, 2, 3 and 4 are produced by 6, 7, 8 and 9 cycles of PCR using GW-A2-FwdP and COBRA-A2-RevP primers from Table 1 with GW-COBRA library material as the template respectively. Lane 5, 6, 7 and 8 are produced by 6, 7, 8 and 9 cycles of PCR using LA-A2+T7-FwdP and COBRA-A2-RevP primers from Table 1 with LA-COBRA library material as the template, respectively; ( C ) Final library products: GW-COBRA and LA-COBRA methylome libraries of HCT116 DNA amplified using flowcell or LADS primer pairs respectively (Lane 2 and 4). Lane 1 and 3 are negative controls for GW-COBRA and LA-COBRA libraries, respectively; ( D ) <t>Bioanalyzer</t> results of GW-COBRA and LA-COBRA methylome sequencing libraries, respectively, prepared from HCT116 cell line DNA. The final library fragments ranged between 150–500 bp with an average size of 257 and 360 bp for GW-COBRA and LA-COBRA, respectively.
    Agilent Bioa Nalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 2056 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 2056 article reviews
    Price from $9.99 to $1999.99
    agilent bioa nalyzer - by Bioz Stars, 2020-04
    94/100 stars
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    92
    Agilent technologies bioa rna integrity number rin
    GW-COBRA and LA-COBRA library construction results. ( A ) Sonicated genomic DNA isolated from HCT116 cell line (lane 1), 50 ng of Adapter-2 ligated library material (lane 2). Column purified Adapter-2 ligated library material (lane 3); ( B ) PCR amplification test of bisulfite treated library materials. Lane 1, 2, 3 and 4 are produced by 6, 7, 8 and 9 cycles of PCR using GW-A2-FwdP and COBRA-A2-RevP primers from Table 1 with GW-COBRA library material as the template respectively. Lane 5, 6, 7 and 8 are produced by 6, 7, 8 and 9 cycles of PCR using LA-A2+T7-FwdP and COBRA-A2-RevP primers from Table 1 with LA-COBRA library material as the template, respectively; ( C ) Final library products: GW-COBRA and LA-COBRA methylome libraries of HCT116 DNA amplified using flowcell or LADS primer pairs respectively (Lane 2 and 4). Lane 1 and 3 are negative controls for GW-COBRA and LA-COBRA libraries, respectively; ( D ) <t>Bioanalyzer</t> results of GW-COBRA and LA-COBRA methylome sequencing libraries, respectively, prepared from HCT116 cell line DNA. The final library fragments ranged between 150–500 bp with an average size of 257 and 360 bp for GW-COBRA and LA-COBRA, respectively.
    Bioa Rna Integrity Number Rin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bioa rna integrity number rin - by Bioz Stars, 2020-04
    92/100 stars
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    95
    Bio-Rad bioanalyzer
    GW-COBRA and LA-COBRA library construction results. ( A ) Sonicated genomic DNA isolated from HCT116 cell line (lane 1), 50 ng of Adapter-2 ligated library material (lane 2). Column purified Adapter-2 ligated library material (lane 3); ( B ) PCR amplification test of bisulfite treated library materials. Lane 1, 2, 3 and 4 are produced by 6, 7, 8 and 9 cycles of PCR using GW-A2-FwdP and COBRA-A2-RevP primers from Table 1 with GW-COBRA library material as the template respectively. Lane 5, 6, 7 and 8 are produced by 6, 7, 8 and 9 cycles of PCR using LA-A2+T7-FwdP and COBRA-A2-RevP primers from Table 1 with LA-COBRA library material as the template, respectively; ( C ) Final library products: GW-COBRA and LA-COBRA methylome libraries of HCT116 DNA amplified using flowcell or LADS primer pairs respectively (Lane 2 and 4). Lane 1 and 3 are negative controls for GW-COBRA and LA-COBRA libraries, respectively; ( D ) <t>Bioanalyzer</t> results of GW-COBRA and LA-COBRA methylome sequencing libraries, respectively, prepared from HCT116 cell line DNA. The final library fragments ranged between 150–500 bp with an average size of 257 and 360 bp for GW-COBRA and LA-COBRA, respectively.
    Bioanalyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer/product/Bio-Rad
    Average 95 stars, based on 191 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2020-04
    95/100 stars
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    91
    Hitachi Ltd bioanalyzer
    GW-COBRA and LA-COBRA library construction results. ( A ) Sonicated genomic DNA isolated from HCT116 cell line (lane 1), 50 ng of Adapter-2 ligated library material (lane 2). Column purified Adapter-2 ligated library material (lane 3); ( B ) PCR amplification test of bisulfite treated library materials. Lane 1, 2, 3 and 4 are produced by 6, 7, 8 and 9 cycles of PCR using GW-A2-FwdP and COBRA-A2-RevP primers from Table 1 with GW-COBRA library material as the template respectively. Lane 5, 6, 7 and 8 are produced by 6, 7, 8 and 9 cycles of PCR using LA-A2+T7-FwdP and COBRA-A2-RevP primers from Table 1 with LA-COBRA library material as the template, respectively; ( C ) Final library products: GW-COBRA and LA-COBRA methylome libraries of HCT116 DNA amplified using flowcell or LADS primer pairs respectively (Lane 2 and 4). Lane 1 and 3 are negative controls for GW-COBRA and LA-COBRA libraries, respectively; ( D ) <t>Bioanalyzer</t> results of GW-COBRA and LA-COBRA methylome sequencing libraries, respectively, prepared from HCT116 cell line DNA. The final library fragments ranged between 150–500 bp with an average size of 257 and 360 bp for GW-COBRA and LA-COBRA, respectively.
    Bioanalyzer, supplied by Hitachi Ltd, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer/product/Hitachi Ltd
    Average 91 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2020-04
    91/100 stars
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    99
    Thermo Fisher bioanalyzer
    <t>Bioanalyzer</t> results of total RNA extracted using the method described in this study. Extractions were made from four different species and tissues as indicated and run on an Agilent 2100 Bioanalyzer using the Total RNA Pico assay (Functional Genomics Laboratory, California Institute for Quantitative Biosciences [QB3], University of California, Berkeley).
    Bioanalyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer/product/Thermo Fisher
    Average 99 stars, based on 1125 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2020-04
    99/100 stars
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    94
    Agilent technologies a bioanalyzer
    <t>Bioanalyzer</t> results of total RNA extracted using the method described in this study. Extractions were made from four different species and tissues as indicated and run on an Agilent 2100 Bioanalyzer using the Total RNA Pico assay (Functional Genomics Laboratory, California Institute for Quantitative Biosciences [QB3], University of California, Berkeley).
    A Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a bioanalyzer/product/Agilent technologies
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    a bioanalyzer - by Bioz Stars, 2020-04
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    93
    Macrogen bioanalyzer
    <t>Bioanalyzer</t> results of total RNA extracted using the method described in this study. Extractions were made from four different species and tissues as indicated and run on an Agilent 2100 Bioanalyzer using the Total RNA Pico assay (Functional Genomics Laboratory, California Institute for Quantitative Biosciences [QB3], University of California, Berkeley).
    Bioanalyzer, supplied by Macrogen, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2020-04
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    91
    Takeda 10 4161 bioa 29766
    <t>Bioanalyzer</t> results of total RNA extracted using the method described in this study. Extractions were made from four different species and tissues as indicated and run on an Agilent 2100 Bioanalyzer using the Total RNA Pico assay (Functional Genomics Laboratory, California Institute for Quantitative Biosciences [QB3], University of California, Berkeley).
    10 4161 Bioa 29766, supplied by Takeda, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 2 article reviews
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    10 4161 bioa 29766 - by Bioz Stars, 2020-04
    91/100 stars
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    87
    Olympus bioanalyzer
    <t>Bioanalyzer</t> results of total RNA extracted using the method described in this study. Extractions were made from four different species and tissues as indicated and run on an Agilent 2100 Bioanalyzer using the Total RNA Pico assay (Functional Genomics Laboratory, California Institute for Quantitative Biosciences [QB3], University of California, Berkeley).
    Bioanalyzer, supplied by Olympus, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 5 article reviews
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    bioanalyzer - by Bioz Stars, 2020-04
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    93
    Seahorse Biosciences bioanalyzer
    Role of PPARα in in vitro browning. (A) Stromal vascular cells of wildtype and PPARα−/− mice were differentiated towards brown-like adipocytes. Relative gene expression of adipogenic markers and brown adipocyte markers as determined by qPCR and expressed in a heatmap. (B) Differentiating human pre-adipocytes derived from subcutaneous WAT were treated with GW7647 (300 nM) for 5 days. Relative gene expression of UCP1 , PDK4 , ADIPOQ , and ACADVL in differentiated adipocytes is shown. The different colors represent the primary adipocytes from 4 different individuals. For each individual, the expression level of the untreated adipocytes was set at 1. (C) Cellular respiration trace measured in adipocytes derived from human WAT on <t>bioanalyzer</t> from Seahorse with GW7647 (open circles) or without GW7647 (closed circles). (D) Basal cellular respiration in adipocytes derived from human WAT with or without GW7647 treatment during differentiation.
    Bioanalyzer, supplied by Seahorse Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer/product/Seahorse Biosciences
    Average 93 stars, based on 7 article reviews
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    bioanalyzer - by Bioz Stars, 2020-04
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    92
    Bioarray bioanalyzer
    Role of PPARα in in vitro browning. (A) Stromal vascular cells of wildtype and PPARα−/− mice were differentiated towards brown-like adipocytes. Relative gene expression of adipogenic markers and brown adipocyte markers as determined by qPCR and expressed in a heatmap. (B) Differentiating human pre-adipocytes derived from subcutaneous WAT were treated with GW7647 (300 nM) for 5 days. Relative gene expression of UCP1 , PDK4 , ADIPOQ , and ACADVL in differentiated adipocytes is shown. The different colors represent the primary adipocytes from 4 different individuals. For each individual, the expression level of the untreated adipocytes was set at 1. (C) Cellular respiration trace measured in adipocytes derived from human WAT on <t>bioanalyzer</t> from Seahorse with GW7647 (open circles) or without GW7647 (closed circles). (D) Basal cellular respiration in adipocytes derived from human WAT with or without GW7647 treatment during differentiation.
    Bioanalyzer, supplied by Bioarray, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2020-04
    92/100 stars
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    99
    Illumina Inc bioanalyzer
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    Bioanalyzer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer/product/Illumina Inc
    Average 99 stars, based on 1320 article reviews
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    bioanalyzer - by Bioz Stars, 2020-04
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    96
    PerkinElmer bioanalyzer
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    Bioanalyzer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 96/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer - by Bioz Stars, 2020-04
    96/100 stars
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    86
    Illumina Inc bioanalyzer bioanalyzer system
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    Bioanalyzer Bioanalyzer System, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer bioanalyzer system/product/Illumina Inc
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer bioanalyzer system - by Bioz Stars, 2020-04
    86/100 stars
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    94
    Agilent technologies 1200 bioanalyzer
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    1200 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 10 article reviews
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    1200 bioanalyzer - by Bioz Stars, 2020-04
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    93
    Agilent technologies 2000 bioanalyzer
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    2000 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    2000 bioanalyzer - by Bioz Stars, 2020-04
    93/100 stars
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    99
    Agilent technologies agilent2100 bioanalyzer
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    Agilent2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 232 article reviews
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    agilent2100 bioanalyzer - by Bioz Stars, 2020-04
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    92
    Agilent technologies bioanalyzer 21000
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    Bioanalyzer 21000, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 21000 - by Bioz Stars, 2020-04
    92/100 stars
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    87
    Agilent technologies bioanalyzer 2100b
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
    Bioanalyzer 2100b, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 87/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer 2100b - by Bioz Stars, 2020-04
    87/100 stars
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    99
    Agilent technologies bioanalyzer 2200
    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a <t>Bioanalyzer</t> total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value
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    Agilent technologies bioanalyzer analysis
    Quality assessment of RNA samples isolated by each method. ( A ) A representative composite <t>bioanalyzer</t> digital gel image using two technical replicates of each of the RNA extraction method tested (see ‘Materials and Methods’ section). ( B ) A representative composite image of technical replicates of 250 ng of total RNA (based on A 260 ) from each RNA extraction method electrophoresed on a 1.2% agarose–0.5× TBE gel and stained with ethidium bromide.
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    Quality assessment of RNA samples isolated by each method. ( A ) A representative composite <t>bioanalyzer</t> digital gel image using two technical replicates of each of the RNA extraction method tested (see ‘Materials and Methods’ section). ( B ) A representative composite image of technical replicates of 250 ng of total RNA (based on A 260 ) from each RNA extraction method electrophoresed on a 1.2% agarose–0.5× TBE gel and stained with ethidium bromide.
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    Quality assessment of RNA samples isolated by each method. ( A ) A representative composite <t>bioanalyzer</t> digital gel image using two technical replicates of each of the RNA extraction method tested (see ‘Materials and Methods’ section). ( B ) A representative composite image of technical replicates of 250 ng of total RNA (based on A 260 ) from each RNA extraction method electrophoresed on a 1.2% agarose–0.5× TBE gel and stained with ethidium bromide.
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    Quality assessment of RNA samples isolated by each method. ( A ) A representative composite <t>bioanalyzer</t> digital gel image using two technical replicates of each of the RNA extraction method tested (see ‘Materials and Methods’ section). ( B ) A representative composite image of technical replicates of 250 ng of total RNA (based on A 260 ) from each RNA extraction method electrophoresed on a 1.2% agarose–0.5× TBE gel and stained with ethidium bromide.
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    Agilent technologies bioanalyzer platform
    Analysis of RNA quality and length for RNA derived from microdissected cells. Representative RNA gel images generated with an RNA 6000 Pico LabChip®on a <t>Bioanalyzer</t> platform are shown for total RNA ( A1 ) and aRNA ( B1 ). Corresponding electropherograms are shown for total RNA ( A2 ) and aRNA ( B2 ). M, marker for sample synchronization; 18S, 18S rRNA; 28S, 28S rRNA; a–f, RNA ladder (0.2, 0.5, 1.0, 2.0, 4.0, 6.0 kb).
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    Agilent technologies bioanalyzer system
    Testing split–Cas9 efficiency in Neuro-2a TLR cell lines. ( A ) Overview of the WT and split–Cas9 expression plasmids used (Cas9, N-Cas9_N-Intein_v1, C-Intein_C-Cas9_v1, N-Cas9_N-Intein_v2, C-Intein_C-Cas9_v2, gRNA crTLR#1/#2). To ensure a high expression; a strong synthetic mammalian promoter (CAG, green) and a bovine growth hormone (bGH, red) polyadenylation site was used. Cas9 cDNA is shown in orange, N-intein in dark brown and C-Intein in light brown. NLS (dark red): Nuclear localization signal. FLAG and HA tag are shown in light and dark grey respectively. For gRNA expression (turquoise), U6 promoter was chosen (dark green). ( B ) Results after FACS: only transfection with both N- and C-terminal parts of the split–intein–Cas9 system for version 1 (v1) and version 2 (v2) resulted in nuclease activity similar to wild-type SpCas9, represented in HDR or NHEJ events; transfection with only one moiety did not show any observable HDR or NHEJ events. Shown are means ± SD of three independent experiments. ( C ) The split-intein-Cas9 system v1 was used to target the fused in sarcoma (Fus) gene's second last exon. The respective segment was PCR amplified with the annotated primers for further analysis. T7 endonuclease I assay was performed after PCR on the samples to investigate the occurrence of NHEJ events. After the assay, the samples were analyzed with a <t>Bioanalyzer.</t> The appearance of a second band indicates the presence of indels resulting from NHEJ events. ( D ) Targeting of Rosa26 locus with the gRNA Rosa26#1. Only indels were detected when SpCas9 wild-type or both SpCas9 moieties were transfected. ( E ) Targeting of Rosa26 locus with the gRNA Rosa26#3. Indels were detected by RFLP analysis. The XbaI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( F ) Targeting of Rab38 locus with the gRNA Rab38#2. Indels were detected by RFLP analysis. The XcmI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( G ) Quantification of the nuclease activity in each target sequence. FU: fluorescence units, s: seconds.
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    Agilent technologies bioanalyzer tapestation
    Testing split–Cas9 efficiency in Neuro-2a TLR cell lines. ( A ) Overview of the WT and split–Cas9 expression plasmids used (Cas9, N-Cas9_N-Intein_v1, C-Intein_C-Cas9_v1, N-Cas9_N-Intein_v2, C-Intein_C-Cas9_v2, gRNA crTLR#1/#2). To ensure a high expression; a strong synthetic mammalian promoter (CAG, green) and a bovine growth hormone (bGH, red) polyadenylation site was used. Cas9 cDNA is shown in orange, N-intein in dark brown and C-Intein in light brown. NLS (dark red): Nuclear localization signal. FLAG and HA tag are shown in light and dark grey respectively. For gRNA expression (turquoise), U6 promoter was chosen (dark green). ( B ) Results after FACS: only transfection with both N- and C-terminal parts of the split–intein–Cas9 system for version 1 (v1) and version 2 (v2) resulted in nuclease activity similar to wild-type SpCas9, represented in HDR or NHEJ events; transfection with only one moiety did not show any observable HDR or NHEJ events. Shown are means ± SD of three independent experiments. ( C ) The split-intein-Cas9 system v1 was used to target the fused in sarcoma (Fus) gene's second last exon. The respective segment was PCR amplified with the annotated primers for further analysis. T7 endonuclease I assay was performed after PCR on the samples to investigate the occurrence of NHEJ events. After the assay, the samples were analyzed with a <t>Bioanalyzer.</t> The appearance of a second band indicates the presence of indels resulting from NHEJ events. ( D ) Targeting of Rosa26 locus with the gRNA Rosa26#1. Only indels were detected when SpCas9 wild-type or both SpCas9 moieties were transfected. ( E ) Targeting of Rosa26 locus with the gRNA Rosa26#3. Indels were detected by RFLP analysis. The XbaI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( F ) Targeting of Rab38 locus with the gRNA Rab38#2. Indels were detected by RFLP analysis. The XcmI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( G ) Quantification of the nuclease activity in each target sequence. FU: fluorescence units, s: seconds.
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    Bio-Rad experion bioanalyzer
    Testing split–Cas9 efficiency in Neuro-2a TLR cell lines. ( A ) Overview of the WT and split–Cas9 expression plasmids used (Cas9, N-Cas9_N-Intein_v1, C-Intein_C-Cas9_v1, N-Cas9_N-Intein_v2, C-Intein_C-Cas9_v2, gRNA crTLR#1/#2). To ensure a high expression; a strong synthetic mammalian promoter (CAG, green) and a bovine growth hormone (bGH, red) polyadenylation site was used. Cas9 cDNA is shown in orange, N-intein in dark brown and C-Intein in light brown. NLS (dark red): Nuclear localization signal. FLAG and HA tag are shown in light and dark grey respectively. For gRNA expression (turquoise), U6 promoter was chosen (dark green). ( B ) Results after FACS: only transfection with both N- and C-terminal parts of the split–intein–Cas9 system for version 1 (v1) and version 2 (v2) resulted in nuclease activity similar to wild-type SpCas9, represented in HDR or NHEJ events; transfection with only one moiety did not show any observable HDR or NHEJ events. Shown are means ± SD of three independent experiments. ( C ) The split-intein-Cas9 system v1 was used to target the fused in sarcoma (Fus) gene's second last exon. The respective segment was PCR amplified with the annotated primers for further analysis. T7 endonuclease I assay was performed after PCR on the samples to investigate the occurrence of NHEJ events. After the assay, the samples were analyzed with a <t>Bioanalyzer.</t> The appearance of a second band indicates the presence of indels resulting from NHEJ events. ( D ) Targeting of Rosa26 locus with the gRNA Rosa26#1. Only indels were detected when SpCas9 wild-type or both SpCas9 moieties were transfected. ( E ) Targeting of Rosa26 locus with the gRNA Rosa26#3. Indels were detected by RFLP analysis. The XbaI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( F ) Targeting of Rab38 locus with the gRNA Rab38#2. Indels were detected by RFLP analysis. The XcmI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( G ) Quantification of the nuclease activity in each target sequence. FU: fluorescence units, s: seconds.
    Experion Bioanalyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Level of MCP-1 secreted by LA-4 murine lung epithelial cell line following B. pseudomallei , KHW infection. LA-4 cells were infected with KHW and control cells were treated with F12K medium. At 0 h, 2 h, 6 h, 24 h and 48 h post infection, supernatants from KHW-infected (▪) and control (▪) cells were harvested and the levels of MCP-1 was measured using <t>FACSArray</t> <t>Bioanalyzer</t> and Cytometric Bead Array. Results are expressed as mean ± standard deviation of the triplicates, and are representation of two independent experiments. ** P
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    Advanced Analytical Inc fragment bioanalyzer
    Level of MCP-1 secreted by LA-4 murine lung epithelial cell line following B. pseudomallei , KHW infection. LA-4 cells were infected with KHW and control cells were treated with F12K medium. At 0 h, 2 h, 6 h, 24 h and 48 h post infection, supernatants from KHW-infected (▪) and control (▪) cells were harvested and the levels of MCP-1 was measured using <t>FACSArray</t> <t>Bioanalyzer</t> and Cytometric Bead Array. Results are expressed as mean ± standard deviation of the triplicates, and are representation of two independent experiments. ** P
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    Image Search Results


    Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Journal: BMC Research Notes

    Article Title: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

    doi: 10.1186/1756-0500-4-217

    Figure Lengend Snippet: Boxplot summary of all data . Semilogarithmic boxplot breakdown of RNA yield for totalRNA, smallRNA and microRNA of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RQI/RIN values of the totalRNA as determined by the instrument software following the Experion and 2100 Bioanalyzer electrophoresis systems are depicted at the bottom. Boxes indicate the 25-75% intervals, squares the mean, and whiskers the standard deviation, where this lies outside the 25-75% interval.

    Article Snippet: The Small RNA Kit (Agilent) was used to analyse smallRNA and miRNA content with the 2100 Bioanalyzer (Agilent).

    Techniques: Software, Electrophoresis, Standard Deviation

    Electropherograms obtained with the Bioanalyzer 2100 in miRNA-Ref and plasma samples. Examples of the profiles obtained from a miRNA-Ref sample at 10 ng/μL ( a , Bio-PicoChip; ( b ), Bio-SmallChip), a miRNA-Ref sample at 1 ng/μL ( c , Bio-PicoChip; ( d ), Bio-SmallChip), and a plasma sample ( e , Bio-PicoChip; ( f ), Bio-SmallChip). The 10 ng/µL miRNA-Ref sample ( a ) was diluted to fit the detection range of the Bio-PicoChip (0.05–5 ng/µL) and the final concentration was calculated by applying the dilution factor to the value obtained by the Bioanalyzer. In the miRNA-Ref samples ( a – d ), the overall profile is consistent with the presence of ribosomic RNA enriched with small RNAs, whereas in plasma samples ( e , f ) only small RNAs, but no long RNAs, are present. All electropherograms include the corresponding quantification (in bold) and the RNA integrity number (RIN) number (in italics) obtained with the Bio-PicoChip ( a , c , e ) or just the quantification (in bold) with the Bio-SmallChip ( b , d , f ).

    Journal: Scientific Reports

    Article Title: Defining quantification methods and optimizing protocols for microarray hybridization of circulating microRNAs

    doi: 10.1038/s41598-017-08134-3

    Figure Lengend Snippet: Electropherograms obtained with the Bioanalyzer 2100 in miRNA-Ref and plasma samples. Examples of the profiles obtained from a miRNA-Ref sample at 10 ng/μL ( a , Bio-PicoChip; ( b ), Bio-SmallChip), a miRNA-Ref sample at 1 ng/μL ( c , Bio-PicoChip; ( d ), Bio-SmallChip), and a plasma sample ( e , Bio-PicoChip; ( f ), Bio-SmallChip). The 10 ng/µL miRNA-Ref sample ( a ) was diluted to fit the detection range of the Bio-PicoChip (0.05–5 ng/µL) and the final concentration was calculated by applying the dilution factor to the value obtained by the Bioanalyzer. In the miRNA-Ref samples ( a – d ), the overall profile is consistent with the presence of ribosomic RNA enriched with small RNAs, whereas in plasma samples ( e , f ) only small RNAs, but no long RNAs, are present. All electropherograms include the corresponding quantification (in bold) and the RNA integrity number (RIN) number (in italics) obtained with the Bio-PicoChip ( a , c , e ) or just the quantification (in bold) with the Bio-SmallChip ( b , d , f ).

    Article Snippet: RNA Integrity The RNA profile and integrity of all samples (inferred by the RIN) was assessed using the Bioanalyzer 2100 (Agilent Technologies) with the Bio-PicoChip and Bio-SmallChip (see Quantification, Agilent 2100 Bioanalyzer).

    Techniques: Concentration Assay

    UV–Vis spectroscopy of PLP or PLP-bound (holo) BioA under various conditions. ( a ) Spectra of 200 µ M holo BioA (purple), 200 µ M PLP in 100 m M Tris pH 7.6 (black), 200 µ M PLP in 100 m

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

    doi: 10.1107/S1744309112012912

    Figure Lengend Snippet: UV–Vis spectroscopy of PLP or PLP-bound (holo) BioA under various conditions. ( a ) Spectra of 200 µ M holo BioA (purple), 200 µ M PLP in 100 m M Tris pH 7.6 (black), 200 µ M PLP in 100 m

    Article Snippet: UV–Vis spectroscopy of material purified using this technique had a peak near 420 nm (Fig. 2 b ) consistent with PLP covalently bound to BioA as a Schiff base.

    Techniques: UV-Vis Spectroscopy, Plasmid Purification

    DSF melting curves, their first derivatives and representative crystallization images for the various stages of purification optimization. Transitions at 318, 341 and 359 K correspond to misfolded, apo and PLP-loaded BioA, respectively. ( a ) BioA

    Journal: Acta Crystallographica Section F: Structural Biology and Crystallization Communications

    Article Title: Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

    doi: 10.1107/S1744309112012912

    Figure Lengend Snippet: DSF melting curves, their first derivatives and representative crystallization images for the various stages of purification optimization. Transitions at 318, 341 and 359 K correspond to misfolded, apo and PLP-loaded BioA, respectively. ( a ) BioA

    Article Snippet: UV–Vis spectroscopy of material purified using this technique had a peak near 420 nm (Fig. 2 b ) consistent with PLP covalently bound to BioA as a Schiff base.

    Techniques: Crystallization Assay, Purification, Plasmid Purification

    GW-COBRA and LA-COBRA library construction results. ( A ) Sonicated genomic DNA isolated from HCT116 cell line (lane 1), 50 ng of Adapter-2 ligated library material (lane 2). Column purified Adapter-2 ligated library material (lane 3); ( B ) PCR amplification test of bisulfite treated library materials. Lane 1, 2, 3 and 4 are produced by 6, 7, 8 and 9 cycles of PCR using GW-A2-FwdP and COBRA-A2-RevP primers from Table 1 with GW-COBRA library material as the template respectively. Lane 5, 6, 7 and 8 are produced by 6, 7, 8 and 9 cycles of PCR using LA-A2+T7-FwdP and COBRA-A2-RevP primers from Table 1 with LA-COBRA library material as the template, respectively; ( C ) Final library products: GW-COBRA and LA-COBRA methylome libraries of HCT116 DNA amplified using flowcell or LADS primer pairs respectively (Lane 2 and 4). Lane 1 and 3 are negative controls for GW-COBRA and LA-COBRA libraries, respectively; ( D ) Bioanalyzer results of GW-COBRA and LA-COBRA methylome sequencing libraries, respectively, prepared from HCT116 cell line DNA. The final library fragments ranged between 150–500 bp with an average size of 257 and 360 bp for GW-COBRA and LA-COBRA, respectively.

    Journal: Genes

    Article Title: COBRA-Seq: Sensitive and Quantitative Methylome Profiling

    doi: 10.3390/genes6041140

    Figure Lengend Snippet: GW-COBRA and LA-COBRA library construction results. ( A ) Sonicated genomic DNA isolated from HCT116 cell line (lane 1), 50 ng of Adapter-2 ligated library material (lane 2). Column purified Adapter-2 ligated library material (lane 3); ( B ) PCR amplification test of bisulfite treated library materials. Lane 1, 2, 3 and 4 are produced by 6, 7, 8 and 9 cycles of PCR using GW-A2-FwdP and COBRA-A2-RevP primers from Table 1 with GW-COBRA library material as the template respectively. Lane 5, 6, 7 and 8 are produced by 6, 7, 8 and 9 cycles of PCR using LA-A2+T7-FwdP and COBRA-A2-RevP primers from Table 1 with LA-COBRA library material as the template, respectively; ( C ) Final library products: GW-COBRA and LA-COBRA methylome libraries of HCT116 DNA amplified using flowcell or LADS primer pairs respectively (Lane 2 and 4). Lane 1 and 3 are negative controls for GW-COBRA and LA-COBRA libraries, respectively; ( D ) Bioanalyzer results of GW-COBRA and LA-COBRA methylome sequencing libraries, respectively, prepared from HCT116 cell line DNA. The final library fragments ranged between 150–500 bp with an average size of 257 and 360 bp for GW-COBRA and LA-COBRA, respectively.

    Article Snippet: Finally, the size distribution was visualized using Agilent DNA 1000 Assay in 2100 Bioanalyzer (Agilent Technologies, Los Angeles, CA, USA) using the manufacturer’s protocol.

    Techniques: Combined Bisulfite Restriction Analysis Assay, Sonication, Isolation, Purification, Polymerase Chain Reaction, Amplification, Produced, Sequencing

    Urinary microvesicle RNA integrity and alignment to the genome. a) RNA isolated from urinary microvesicles was shown to be of high integrity with prominent 18S and 28S rRNA peaks when analyzed using the Agilent Bioanalyzer. Red trace 1.7 ng RNA with DNase, Blue trace 2.2 ng RNA without DNase. b) Flow chart outlining sample processing. An initial RNase and DNase digestion was carried out to remove extraneous nucleic acids co-isolating with the microvesicle pellet. To determine the proportion of potential DNA inside the microvesicles the extracted RNA was divided into two groups; No DNase digestion (-DNase), which yields RNA+DNA and DNase digested (+DNase) which yields RNA. c) Both the -DNase and the +DNase samples showed a similar trend in read distribution with ∼88% of reads mapping to rRNA, ∼4% mapping to genes and ∼6% mapping to ncRNA. A smaller proportion of reads (

    Journal: PLoS ONE

    Article Title: Massively Parallel Sequencing of Human Urinary Exosome/Microvesicle RNA Reveals a Predominance of Non-Coding RNA

    doi: 10.1371/journal.pone.0096094

    Figure Lengend Snippet: Urinary microvesicle RNA integrity and alignment to the genome. a) RNA isolated from urinary microvesicles was shown to be of high integrity with prominent 18S and 28S rRNA peaks when analyzed using the Agilent Bioanalyzer. Red trace 1.7 ng RNA with DNase, Blue trace 2.2 ng RNA without DNase. b) Flow chart outlining sample processing. An initial RNase and DNase digestion was carried out to remove extraneous nucleic acids co-isolating with the microvesicle pellet. To determine the proportion of potential DNA inside the microvesicles the extracted RNA was divided into two groups; No DNase digestion (-DNase), which yields RNA+DNA and DNase digested (+DNase) which yields RNA. c) Both the -DNase and the +DNase samples showed a similar trend in read distribution with ∼88% of reads mapping to rRNA, ∼4% mapping to genes and ∼6% mapping to ncRNA. A smaller proportion of reads (

    Article Snippet: Isolated RNA was analyzed on a RNA Pico 6000 chip (Agilent, CA) using an Agilent Bioanalyzer to check for integrity.

    Techniques: Isolation, Flow Cytometry

    RNA exists in RC-MVs. ( A ) MV RNA was compared to cell RNA. In a 2% agarose gel, RC-MV RNA was heterogeneous in size but contained little or no large ribosomal RNA species (18S- and 28S-rRNA) compared to parent cells. Enriched small RNAs were observed in MVs. ( B ) A similar pattern was observed in the Bioanalyzer. ( C ) RC-MVs were treated by RNase A before RNA extraction by TRIzol. No significant differences in the RNA:protein ratios of MVs with or without RNase pretreatment (n = 6) were observed. ( D ) RNase was not able to digest the RNA component in MVs. However, it was able to digest the total cell RNA. When the MVs were pre-treated with the membrane detergent Triton X-100, RNA degradation was observed, suggesting that RNA in RC-MVs is protected by the intact lipid membrane.

    Journal: Scientific Reports

    Article Title: MicroRNA Contents in Matrix Vesicles Produced by Growth Plate Chondrocytes are Cell Maturation Dependent

    doi: 10.1038/s41598-018-21517-4

    Figure Lengend Snippet: RNA exists in RC-MVs. ( A ) MV RNA was compared to cell RNA. In a 2% agarose gel, RC-MV RNA was heterogeneous in size but contained little or no large ribosomal RNA species (18S- and 28S-rRNA) compared to parent cells. Enriched small RNAs were observed in MVs. ( B ) A similar pattern was observed in the Bioanalyzer. ( C ) RC-MVs were treated by RNase A before RNA extraction by TRIzol. No significant differences in the RNA:protein ratios of MVs with or without RNase pretreatment (n = 6) were observed. ( D ) RNase was not able to digest the RNA component in MVs. However, it was able to digest the total cell RNA. When the MVs were pre-treated with the membrane detergent Triton X-100, RNA degradation was observed, suggesting that RNA in RC-MVs is protected by the intact lipid membrane.

    Article Snippet: Bioanalyzer analyses were performed using 300 ng RNA with an RNA 600 Nano Kit (Agilent) and an Agilent 2100 Bioanalyzer (Agilent) according to manufacturer’s protocol.

    Techniques: Agarose Gel Electrophoresis, RNA Extraction

    Gel electrophoresis to separate high molecular weight DNA from the unligated adaptor: A. Example of the agarose gel after electrophoresis. The boxed region is excised and treated with agarase to retrieve the DNA. B. Bioanalyzer (Agilent) lane profile showing the distribution of the genomic DNA after Covaris shearing (∼100–700 bp).

    Journal: Methods in enzymology

    Article Title: S1-seq assay for mapping processed DNA ends

    doi: 10.1016/bs.mie.2017.11.031

    Figure Lengend Snippet: Gel electrophoresis to separate high molecular weight DNA from the unligated adaptor: A. Example of the agarose gel after electrophoresis. The boxed region is excised and treated with agarase to retrieve the DNA. B. Bioanalyzer (Agilent) lane profile showing the distribution of the genomic DNA after Covaris shearing (∼100–700 bp).

    Article Snippet: To verify the size range of the library, a dilution of the library is analyzed on a Bioanalyzer Instrument (Agilent Technologies) according to manufacturer's instruction.

    Techniques: Nucleic Acid Electrophoresis, Molecular Weight, Agarose Gel Electrophoresis, Electrophoresis

    PCR (sequencing libraries) of a wild-type time course. A. Samples from different time points (0, 2, 4 and 6 h) were resolved on a 5% non-denaturing polyacrylamide gel. The first lane is a control where the non-biotinylated P5 adaptor was used. The smear at 200–700 bp (brackets) indicates PCR-amplified adaptor-ligated fragments and is excised from the gel. The DNA is extracted and ethanol precipitated prior to next-generation sequencing. B. Bioanalyzer (Agilent) lane profile showing the S1-seq library size distribution (∼150–600 bp).

    Journal: Methods in enzymology

    Article Title: S1-seq assay for mapping processed DNA ends

    doi: 10.1016/bs.mie.2017.11.031

    Figure Lengend Snippet: PCR (sequencing libraries) of a wild-type time course. A. Samples from different time points (0, 2, 4 and 6 h) were resolved on a 5% non-denaturing polyacrylamide gel. The first lane is a control where the non-biotinylated P5 adaptor was used. The smear at 200–700 bp (brackets) indicates PCR-amplified adaptor-ligated fragments and is excised from the gel. The DNA is extracted and ethanol precipitated prior to next-generation sequencing. B. Bioanalyzer (Agilent) lane profile showing the S1-seq library size distribution (∼150–600 bp).

    Article Snippet: To verify the size range of the library, a dilution of the library is analyzed on a Bioanalyzer Instrument (Agilent Technologies) according to manufacturer's instruction.

    Techniques: Polymerase Chain Reaction, Sequencing, Amplification, Next-Generation Sequencing

    Oligodendrocytes were purified directly from the whole brain of postnatal day 7 rats using flow cytometry. A , FACS density scatter plot in pseudocolor shows forward scatter (FSC) versus side scatter (SSC) properties used to identify viable cells (gate R1). B , A2B5 and O4 immunofluorescence signals were used in combination with gate R1 to sort-purify homogeneous populations of vital cells at early and later stages of oligodendrocyte differentiation. Gates were set to collect single A2B5 + progenitors expressing middle to high levels of A2B5 (top left quadrant, R2) and single O4 + oligodendrocytes (bottom right quadrant, R3). C , Agilent Bioanalyzer analysis of total RNA extracted from oligodendrocytes isolated by FACS demonstrates the intact 28S and 18S rRNA bands. D , Sorted A2B5 + cells cultured for 6 d differentiated into mature oligodendrocytes, as evidenced by strong immunoreactivity with the O1 antibody (red fluorescence). Scale bar, 10 μm.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Identification of a Novel Oligodendrocyte Cell Adhesion Protein Using Gene Expression Profiling

    doi: 10.1523/JNEUROSCI.2246-06.2006

    Figure Lengend Snippet: Oligodendrocytes were purified directly from the whole brain of postnatal day 7 rats using flow cytometry. A , FACS density scatter plot in pseudocolor shows forward scatter (FSC) versus side scatter (SSC) properties used to identify viable cells (gate R1). B , A2B5 and O4 immunofluorescence signals were used in combination with gate R1 to sort-purify homogeneous populations of vital cells at early and later stages of oligodendrocyte differentiation. Gates were set to collect single A2B5 + progenitors expressing middle to high levels of A2B5 (top left quadrant, R2) and single O4 + oligodendrocytes (bottom right quadrant, R3). C , Agilent Bioanalyzer analysis of total RNA extracted from oligodendrocytes isolated by FACS demonstrates the intact 28S and 18S rRNA bands. D , Sorted A2B5 + cells cultured for 6 d differentiated into mature oligodendrocytes, as evidenced by strong immunoreactivity with the O1 antibody (red fluorescence). Scale bar, 10 μm.

    Article Snippet: The quality of total RNA was assessed using a Bioanalyzer microchip (Agilent, Palo Alto, CA).

    Techniques: Purification, Flow Cytometry, Cytometry, FACS, Immunofluorescence, Expressing, Isolation, Cell Culture, Fluorescence

    Characterization of the ~300 nt long RNA species in Dosidicus gigas giant axoplasm. ( a ) Enrichment of the ~300 nt RNA species. Bioanalyzer profile of axonal RNA after rRNA depletion from giant axon total RNA shows a substantial enrichment of peak (iii). M: RNA marker (25 nt). ( b ) Predicted secondary structure of the large S domain (62–281 nts) for Dosidicus gigas signal recognition particle (SRP) RNA. Conserved and deviant nucleotides are represented in red and black, respectively.

    Journal: Scientific Reports

    Article Title: Demonstration of ion channel synthesis by isolated squid giant axon provides functional evidence for localized axonal membrane protein translation

    doi: 10.1038/s41598-018-20684-8

    Figure Lengend Snippet: Characterization of the ~300 nt long RNA species in Dosidicus gigas giant axoplasm. ( a ) Enrichment of the ~300 nt RNA species. Bioanalyzer profile of axonal RNA after rRNA depletion from giant axon total RNA shows a substantial enrichment of peak (iii). M: RNA marker (25 nt). ( b ) Predicted secondary structure of the large S domain (62–281 nts) for Dosidicus gigas signal recognition particle (SRP) RNA. Conserved and deviant nucleotides are represented in red and black, respectively.

    Article Snippet: Representative bioanalyzer (Agilent 2000) profile of total RNA from squid giant axoplasm.

    Techniques: Marker

    Bioanalyzer results of total RNA extracted using the method described in this study. Extractions were made from four different species and tissues as indicated and run on an Agilent 2100 Bioanalyzer using the Total RNA Pico assay (Functional Genomics Laboratory, California Institute for Quantitative Biosciences [QB3], University of California, Berkeley).

    Journal: Applications in Plant Sciences

    Article Title: A method for extracting high-quality RNA from diverse plants for next-generation sequencing and gene expression analyses 1

    doi: 10.3732/apps.1300070

    Figure Lengend Snippet: Bioanalyzer results of total RNA extracted using the method described in this study. Extractions were made from four different species and tissues as indicated and run on an Agilent 2100 Bioanalyzer using the Total RNA Pico assay (Functional Genomics Laboratory, California Institute for Quantitative Biosciences [QB3], University of California, Berkeley).

    Article Snippet: While a more accurate assessment of the quality will be determined with a bioanalyzer prior to sequencing, this initial NanoDrop read will provide an indication of the presence of RNA, enabling the researcher to continue.

    Techniques: Functional Assay

    Role of PPARα in in vitro browning. (A) Stromal vascular cells of wildtype and PPARα−/− mice were differentiated towards brown-like adipocytes. Relative gene expression of adipogenic markers and brown adipocyte markers as determined by qPCR and expressed in a heatmap. (B) Differentiating human pre-adipocytes derived from subcutaneous WAT were treated with GW7647 (300 nM) for 5 days. Relative gene expression of UCP1 , PDK4 , ADIPOQ , and ACADVL in differentiated adipocytes is shown. The different colors represent the primary adipocytes from 4 different individuals. For each individual, the expression level of the untreated adipocytes was set at 1. (C) Cellular respiration trace measured in adipocytes derived from human WAT on bioanalyzer from Seahorse with GW7647 (open circles) or without GW7647 (closed circles). (D) Basal cellular respiration in adipocytes derived from human WAT with or without GW7647 treatment during differentiation.

    Journal: Molecular Metabolism

    Article Title: The Peroxisome Proliferator-Activated Receptor α is dispensable for cold-induced adipose tissue browning in mice

    doi: 10.1016/j.molmet.2018.01.023

    Figure Lengend Snippet: Role of PPARα in in vitro browning. (A) Stromal vascular cells of wildtype and PPARα−/− mice were differentiated towards brown-like adipocytes. Relative gene expression of adipogenic markers and brown adipocyte markers as determined by qPCR and expressed in a heatmap. (B) Differentiating human pre-adipocytes derived from subcutaneous WAT were treated with GW7647 (300 nM) for 5 days. Relative gene expression of UCP1 , PDK4 , ADIPOQ , and ACADVL in differentiated adipocytes is shown. The different colors represent the primary adipocytes from 4 different individuals. For each individual, the expression level of the untreated adipocytes was set at 1. (C) Cellular respiration trace measured in adipocytes derived from human WAT on bioanalyzer from Seahorse with GW7647 (open circles) or without GW7647 (closed circles). (D) Basal cellular respiration in adipocytes derived from human WAT with or without GW7647 treatment during differentiation.

    Article Snippet: To determine NE-stimulated mitochondrial uncoupling, oxygen consumption was measured using bioanalyzer from Seahorse Bioscience after addition of 2 μM oligomycin, which inhibited ATPase, followed by 1 μM NE.

    Techniques: In Vitro, Mouse Assay, Expressing, Real-time Polymerase Chain Reaction, Derivative Assay

    Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a Bioanalyzer total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value

    Journal: International Journal of Molecular Sciences

    Article Title: Evaluation and Adaptation of a Laboratory-Based cDNA Library Preparation Protocol for Retrospective Sequencing of Archived MicroRNAs from up to 35-Year-Old Clinical FFPE Specimens

    doi: 10.3390/ijms18030627

    Figure Lengend Snippet: Evaluation of the optimized barcoded cDNA library preparation protocol with matched frozen and FFPE RNA samples. ( A ) Distance mapping of miRNA sequencing expression using the Euclidean distance metric for four pairs of matched frozen and FFPE specimens. Matched frozen (Fr-Cx) and eight-year-old FFPE (Ff-Cx) cervix tissue; matched frozen (Fr-Bngn) and four-year-old FFPE (Ff-Bngn) benign breast tissue; matched frozen (Fr-IBC1) and four-year-old FFPE (Ff-IBC1) invasive ductal carcinoma breast tissue #1; and matched frozen (Fr-IBC1) and eight-year-old FFPE (Ff-IBC2) invasive ductal carcinoma breast tissue #2 were analyzed; ( B ) RNA quality evaluation on a Bioanalyzer total RNA nano chip of matched frozen (Lanes 1 and 2) and FFPE (Lanes 3 and 4) IBC tissue pairs; ( C ) Scatter plot comparisons between matched frozen and FFPE IBC Tissues 1 and 2 (top right and left plots); ( D ) Correlation between IBC2/IBC1 fold-change in matched FFPE and frozen specimens, represented by abundance of miRNAs (log count per million (CPM), red to blue color) and significance of the expression difference between the two tissue pairs (Likelihood Ratio, values above 10 being significant ( p -value

    Article Snippet: After library purification, we used a Bioanalyzer to evaluate library size and purity, prior to sequencing on an Illumina HiSeq 2500.

    Techniques: cDNA Library Assay, Formalin-fixed Paraffin-Embedded, Sequencing, Expressing, Chromatin Immunoprecipitation

    Quality assessment of RNA samples isolated by each method. ( A ) A representative composite bioanalyzer digital gel image using two technical replicates of each of the RNA extraction method tested (see ‘Materials and Methods’ section). ( B ) A representative composite image of technical replicates of 250 ng of total RNA (based on A 260 ) from each RNA extraction method electrophoresed on a 1.2% agarose–0.5× TBE gel and stained with ethidium bromide.

    Journal: Nucleic Acids Research

    Article Title: RNAsnap(TM): a rapid, quantitative and inexpensive, method for isolating total RNA from bacteria

    doi: 10.1093/nar/gks680

    Figure Lengend Snippet: Quality assessment of RNA samples isolated by each method. ( A ) A representative composite bioanalyzer digital gel image using two technical replicates of each of the RNA extraction method tested (see ‘Materials and Methods’ section). ( B ) A representative composite image of technical replicates of 250 ng of total RNA (based on A 260 ) from each RNA extraction method electrophoresed on a 1.2% agarose–0.5× TBE gel and stained with ethidium bromide.

    Article Snippet: As shown in , the quality of the RNA derived using the RNAsnap ™ method was as good or better than RNA obtained by the other methods tested based on both bioanalyzer analysis ( A and ) and agarose gel electrophoresis ( B).

    Techniques: Isolation, RNA Extraction, Staining

    Analysis of RNA quality and length for RNA derived from microdissected cells. Representative RNA gel images generated with an RNA 6000 Pico LabChip®on a Bioanalyzer platform are shown for total RNA ( A1 ) and aRNA ( B1 ). Corresponding electropherograms are shown for total RNA ( A2 ) and aRNA ( B2 ). M, marker for sample synchronization; 18S, 18S rRNA; 28S, 28S rRNA; a–f, RNA ladder (0.2, 0.5, 1.0, 2.0, 4.0, 6.0 kb).

    Journal: Nucleic Acids Research

    Article Title: aRNA-longSAGE: a new approach to generate SAGE libraries from microdissected cells

    doi: 10.1093/nar/gnh130

    Figure Lengend Snippet: Analysis of RNA quality and length for RNA derived from microdissected cells. Representative RNA gel images generated with an RNA 6000 Pico LabChip®on a Bioanalyzer platform are shown for total RNA ( A1 ) and aRNA ( B1 ). Corresponding electropherograms are shown for total RNA ( A2 ) and aRNA ( B2 ). M, marker for sample synchronization; 18S, 18S rRNA; 28S, 28S rRNA; a–f, RNA ladder (0.2, 0.5, 1.0, 2.0, 4.0, 6.0 kb).

    Article Snippet: The quality of the RNA and aRNA was assessed and the amount of aRNA was estimated with an RNA 6000 Pico LabChip® on a Bioanalyzer platform (Agilent, Böblingen, Germany).

    Techniques: Derivative Assay, Generated, Marker

    Testing split–Cas9 efficiency in Neuro-2a TLR cell lines. ( A ) Overview of the WT and split–Cas9 expression plasmids used (Cas9, N-Cas9_N-Intein_v1, C-Intein_C-Cas9_v1, N-Cas9_N-Intein_v2, C-Intein_C-Cas9_v2, gRNA crTLR#1/#2). To ensure a high expression; a strong synthetic mammalian promoter (CAG, green) and a bovine growth hormone (bGH, red) polyadenylation site was used. Cas9 cDNA is shown in orange, N-intein in dark brown and C-Intein in light brown. NLS (dark red): Nuclear localization signal. FLAG and HA tag are shown in light and dark grey respectively. For gRNA expression (turquoise), U6 promoter was chosen (dark green). ( B ) Results after FACS: only transfection with both N- and C-terminal parts of the split–intein–Cas9 system for version 1 (v1) and version 2 (v2) resulted in nuclease activity similar to wild-type SpCas9, represented in HDR or NHEJ events; transfection with only one moiety did not show any observable HDR or NHEJ events. Shown are means ± SD of three independent experiments. ( C ) The split-intein-Cas9 system v1 was used to target the fused in sarcoma (Fus) gene's second last exon. The respective segment was PCR amplified with the annotated primers for further analysis. T7 endonuclease I assay was performed after PCR on the samples to investigate the occurrence of NHEJ events. After the assay, the samples were analyzed with a Bioanalyzer. The appearance of a second band indicates the presence of indels resulting from NHEJ events. ( D ) Targeting of Rosa26 locus with the gRNA Rosa26#1. Only indels were detected when SpCas9 wild-type or both SpCas9 moieties were transfected. ( E ) Targeting of Rosa26 locus with the gRNA Rosa26#3. Indels were detected by RFLP analysis. The XbaI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( F ) Targeting of Rab38 locus with the gRNA Rab38#2. Indels were detected by RFLP analysis. The XcmI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( G ) Quantification of the nuclease activity in each target sequence. FU: fluorescence units, s: seconds.

    Journal: Nucleic Acids Research

    Article Title: Development of an intein-mediated split–Cas9 system for gene therapy

    doi: 10.1093/nar/gkv601

    Figure Lengend Snippet: Testing split–Cas9 efficiency in Neuro-2a TLR cell lines. ( A ) Overview of the WT and split–Cas9 expression plasmids used (Cas9, N-Cas9_N-Intein_v1, C-Intein_C-Cas9_v1, N-Cas9_N-Intein_v2, C-Intein_C-Cas9_v2, gRNA crTLR#1/#2). To ensure a high expression; a strong synthetic mammalian promoter (CAG, green) and a bovine growth hormone (bGH, red) polyadenylation site was used. Cas9 cDNA is shown in orange, N-intein in dark brown and C-Intein in light brown. NLS (dark red): Nuclear localization signal. FLAG and HA tag are shown in light and dark grey respectively. For gRNA expression (turquoise), U6 promoter was chosen (dark green). ( B ) Results after FACS: only transfection with both N- and C-terminal parts of the split–intein–Cas9 system for version 1 (v1) and version 2 (v2) resulted in nuclease activity similar to wild-type SpCas9, represented in HDR or NHEJ events; transfection with only one moiety did not show any observable HDR or NHEJ events. Shown are means ± SD of three independent experiments. ( C ) The split-intein-Cas9 system v1 was used to target the fused in sarcoma (Fus) gene's second last exon. The respective segment was PCR amplified with the annotated primers for further analysis. T7 endonuclease I assay was performed after PCR on the samples to investigate the occurrence of NHEJ events. After the assay, the samples were analyzed with a Bioanalyzer. The appearance of a second band indicates the presence of indels resulting from NHEJ events. ( D ) Targeting of Rosa26 locus with the gRNA Rosa26#1. Only indels were detected when SpCas9 wild-type or both SpCas9 moieties were transfected. ( E ) Targeting of Rosa26 locus with the gRNA Rosa26#3. Indels were detected by RFLP analysis. The XbaI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( F ) Targeting of Rab38 locus with the gRNA Rab38#2. Indels were detected by RFLP analysis. The XcmI resistant product could be only observed when SpCas9 wild-type or both SpCas9 moieties were transfected. ( G ) Quantification of the nuclease activity in each target sequence. FU: fluorescence units, s: seconds.

    Article Snippet: In all cases the amplicons were quantified on 2100 Bioanalyzer system (Agilent Technologies).

    Techniques: Expressing, FACS, Transfection, Activity Assay, Non-Homologous End Joining, Polymerase Chain Reaction, Amplification, T7EI Assay, Sequencing, Fluorescence

    Level of MCP-1 secreted by LA-4 murine lung epithelial cell line following B. pseudomallei , KHW infection. LA-4 cells were infected with KHW and control cells were treated with F12K medium. At 0 h, 2 h, 6 h, 24 h and 48 h post infection, supernatants from KHW-infected (▪) and control (▪) cells were harvested and the levels of MCP-1 was measured using FACSArray Bioanalyzer and Cytometric Bead Array. Results are expressed as mean ± standard deviation of the triplicates, and are representation of two independent experiments. ** P

    Journal: PLoS ONE

    Article Title: Innate Immune Responses of Pulmonary Epithelial Cells to Burkholderia pseudomallei Infection

    doi: 10.1371/journal.pone.0007308

    Figure Lengend Snippet: Level of MCP-1 secreted by LA-4 murine lung epithelial cell line following B. pseudomallei , KHW infection. LA-4 cells were infected with KHW and control cells were treated with F12K medium. At 0 h, 2 h, 6 h, 24 h and 48 h post infection, supernatants from KHW-infected (▪) and control (▪) cells were harvested and the levels of MCP-1 was measured using FACSArray Bioanalyzer and Cytometric Bead Array. Results are expressed as mean ± standard deviation of the triplicates, and are representation of two independent experiments. ** P

    Article Snippet: Cytokine assays The presence of IL-10, IL-12p70, IFNγ, IL-6, MCP-1 and TNFα in the supernatants was analyzed using FACSArray Bioanalyzer and Cytometric Bead Array according to the manufacturer's instructions (BD Biosciences, San Diego, CA).

    Techniques: Infection, Standard Deviation

    Levels of cytokines and chemokines secreted by primary murine lung epithelial cells from (A) BALB/c mice, and (B) C57Bl/6 mice following B. pseudomallei , KHW infection. Primary lung epithelial cells were infected with KHW and control cells were treated with F12K medium. At 0 h, 2 h, 6 h, 24 h and 48 h post infection, supernatants from KHW-infected (▪) and control (▪) cells were harvested and the levels of (A) IL-6, (B) MCP-1 and (C) TNFα were measured using FACSArray Bioanalyzer and Cytometric Bead Array. Results are expressed as mean ± standard deviation of the triplicates, and are representation of three independent experiments. ** P

    Journal: PLoS ONE

    Article Title: Innate Immune Responses of Pulmonary Epithelial Cells to Burkholderia pseudomallei Infection

    doi: 10.1371/journal.pone.0007308

    Figure Lengend Snippet: Levels of cytokines and chemokines secreted by primary murine lung epithelial cells from (A) BALB/c mice, and (B) C57Bl/6 mice following B. pseudomallei , KHW infection. Primary lung epithelial cells were infected with KHW and control cells were treated with F12K medium. At 0 h, 2 h, 6 h, 24 h and 48 h post infection, supernatants from KHW-infected (▪) and control (▪) cells were harvested and the levels of (A) IL-6, (B) MCP-1 and (C) TNFα were measured using FACSArray Bioanalyzer and Cytometric Bead Array. Results are expressed as mean ± standard deviation of the triplicates, and are representation of three independent experiments. ** P

    Article Snippet: Cytokine assays The presence of IL-10, IL-12p70, IFNγ, IL-6, MCP-1 and TNFα in the supernatants was analyzed using FACSArray Bioanalyzer and Cytometric Bead Array according to the manufacturer's instructions (BD Biosciences, San Diego, CA).

    Techniques: Mouse Assay, Infection, Standard Deviation