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  • 99
    Thermo Fisher bigdye terminator v3 1 cycle sequencing kit
    DNA cleavage and strand exchange by SprA. ( A ) Direct sequencing of the intermediates of the SprA-mediated integration reaction. The intermediates: the left and right half-sites of attP , were directly sequenced using a <t>BigDye</t> Terminator <t>v3.1</t> Cycle Sequencing Kit and an ABI 3500 DNA analyzer. The 16 bp overlapping sequence is underlined. The 3΄-end nucleotides indicated by the circles are adenines added by the terminal transferase activity of Taq polymerase used for the cycle sequencing reaction. The cleavage point of attP is shown to the left (indicated by the lines and triangles). ( B ) Effects of point mutations of the central dinucleotides on DNA recombination. Point mutations were introduced into the AAA nucleotides at the center of the attB site (A→T). In vitro integration recombination was performed using 0.5 μM SprA and 50 ng each of the wild-type attP and the mutated attB substrates.
    Bigdye Terminator V3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 25518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v3 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 25518 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v3 1 cycle sequencing kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher bigdye terminator v1 1 cycle sequencing kit
    The DNA sequencing of rs840088. Direct sequencing of a subgroup of samples with the same primers for PCR-RFLP was performed using the <t>BigDye</t> Terminator <t>v1.1</t> Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, USA). (A) GG genotype. (B) GA genotype. (C) AA genotype. 82×48 mm (300×300 DPI).
    Bigdye Terminator V1 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v1 1 cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 5191 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v1 1 cycle sequencing kit - by Bioz Stars, 2020-04
    99/100 stars
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    96
    Thermo Fisher bigdye terminator kit
    The DNA sequencing of rs840088. Direct sequencing of a subgroup of samples with the same primers for PCR-RFLP was performed using the <t>BigDye</t> Terminator <t>v1.1</t> Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, USA). (A) GG genotype. (B) GA genotype. (C) AA genotype. 82×48 mm (300×300 DPI).
    Bigdye Terminator Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator kit/product/Thermo Fisher
    Average 96 stars, based on 150 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator kit - by Bioz Stars, 2020-04
    96/100 stars
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    99
    Thermo Fisher bigdye terminator cycle sequencing kit
    The DNA sequencing of rs840088. Direct sequencing of a subgroup of samples with the same primers for PCR-RFLP was performed using the <t>BigDye</t> Terminator <t>v1.1</t> Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, USA). (A) GG genotype. (B) GA genotype. (C) AA genotype. 82×48 mm (300×300 DPI).
    Bigdye Terminator Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator cycle sequencing kit/product/Thermo Fisher
    Average 99 stars, based on 8506 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator cycle sequencing kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher bigdye terminator v1 1 kit
    The DNA sequencing of rs840088. Direct sequencing of a subgroup of samples with the same primers for PCR-RFLP was performed using the <t>BigDye</t> Terminator <t>v1.1</t> Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, USA). (A) GG genotype. (B) GA genotype. (C) AA genotype. 82×48 mm (300×300 DPI).
    Bigdye Terminator V1 1 Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v1 1 kit/product/Thermo Fisher
    Average 94 stars, based on 188 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v1 1 kit - by Bioz Stars, 2020-04
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    94
    Thermo Fisher bigdye terminator version 3 1 cycle sequencing kit
    CAST promoter activity and calpastatin isoform expression in MG cells. A , RLM RACE was carried out using 5 μg of total RNA isolated from NFI-hyperphosphorylated (T98) or NFI-hypophosphorylated (U251) MG cells. Antisense primer targeting exon 14 of the CAST gene was used for reverse transcription. Nested PCR amplification was carried out with 5′-ligated outer and inner primers and antisense primers targeting exons 8 and 4 of the CAST gene, respectively. PCR products were electrophoresed in 3% Metaphor (FMC Bioproducts) agarose gel, visualized with ethidium bromide, excised, purified, and sequenced using the <t>BigDye</t> Terminator version 3.1 cycle sequencing kit (Thermo Fisher Scientific). Exon composition of the isolated DNA products based on sequence analysis is indicated on the right. B , domain composition of XL-containing (full-length/type II) and XL-less (type III) calpastatin isoforms. T98 ( C ) and U251 ( D ) MG cells were transiently transfected with luciferase reporter constructs containing the CP (∼2000 bp upstream of CAST exon 1), ALT (in intron 3 containing the NFI-binding sites; ∼4000 bp upstream of CAST exon 4), or empty vector (CNT). Luciferase activity was measured using the Luciferase Assay System (Promega) and the FLUOstar microplate reader (BMG LABTECH). Relative -fold change was calculated relative to the empty vector control. Scatter plots show data from three independent experiments. E , U251 MG cells were transiently transfected with luciferase reporter constructs containing the WT ALT or constructs containing mutation at C2 (ALT-C2*), C3 (ALt-C3*), or both C2 and C3 (ALT-C2*C3*) NFI-binding sites. Luciferase activity was measured 48 h post-transfection as described above. p values were obtained from one-way analysis of variance statistical analysis of three independent experiments. *, p
    Bigdye Terminator Version 3 1 Cycle Sequencing Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2010 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator version 3 1 cycle sequencing kit/product/Thermo Fisher
    Average 94 stars, based on 2010 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator version 3 1 cycle sequencing kit - by Bioz Stars, 2020-04
    94/100 stars
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    93
    Thermo Fisher bigdye terminator v3 1 cycle sequencing kit protocol
    CAST promoter activity and calpastatin isoform expression in MG cells. A , RLM RACE was carried out using 5 μg of total RNA isolated from NFI-hyperphosphorylated (T98) or NFI-hypophosphorylated (U251) MG cells. Antisense primer targeting exon 14 of the CAST gene was used for reverse transcription. Nested PCR amplification was carried out with 5′-ligated outer and inner primers and antisense primers targeting exons 8 and 4 of the CAST gene, respectively. PCR products were electrophoresed in 3% Metaphor (FMC Bioproducts) agarose gel, visualized with ethidium bromide, excised, purified, and sequenced using the <t>BigDye</t> Terminator version 3.1 cycle sequencing kit (Thermo Fisher Scientific). Exon composition of the isolated DNA products based on sequence analysis is indicated on the right. B , domain composition of XL-containing (full-length/type II) and XL-less (type III) calpastatin isoforms. T98 ( C ) and U251 ( D ) MG cells were transiently transfected with luciferase reporter constructs containing the CP (∼2000 bp upstream of CAST exon 1), ALT (in intron 3 containing the NFI-binding sites; ∼4000 bp upstream of CAST exon 4), or empty vector (CNT). Luciferase activity was measured using the Luciferase Assay System (Promega) and the FLUOstar microplate reader (BMG LABTECH). Relative -fold change was calculated relative to the empty vector control. Scatter plots show data from three independent experiments. E , U251 MG cells were transiently transfected with luciferase reporter constructs containing the WT ALT or constructs containing mutation at C2 (ALT-C2*), C3 (ALt-C3*), or both C2 and C3 (ALT-C2*C3*) NFI-binding sites. Luciferase activity was measured 48 h post-transfection as described above. p values were obtained from one-way analysis of variance statistical analysis of three independent experiments. *, p
    Bigdye Terminator V3 1 Cycle Sequencing Kit Protocol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bigdye terminator v3 1 cycle sequencing kit protocol/product/Thermo Fisher
    Average 93 stars, based on 107 article reviews
    Price from $9.99 to $1999.99
    bigdye terminator v3 1 cycle sequencing kit protocol - by Bioz Stars, 2020-04
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    Image Search Results


    DNA cleavage and strand exchange by SprA. ( A ) Direct sequencing of the intermediates of the SprA-mediated integration reaction. The intermediates: the left and right half-sites of attP , were directly sequenced using a BigDye Terminator v3.1 Cycle Sequencing Kit and an ABI 3500 DNA analyzer. The 16 bp overlapping sequence is underlined. The 3΄-end nucleotides indicated by the circles are adenines added by the terminal transferase activity of Taq polymerase used for the cycle sequencing reaction. The cleavage point of attP is shown to the left (indicated by the lines and triangles). ( B ) Effects of point mutations of the central dinucleotides on DNA recombination. Point mutations were introduced into the AAA nucleotides at the center of the attB site (A→T). In vitro integration recombination was performed using 0.5 μM SprA and 50 ng each of the wild-type attP and the mutated attB substrates.

    Journal: Nucleic Acids Research

    Article Title: Mechanism of bacterial gene rearrangement: SprA-catalyzed precise DNA recombination and its directionality control by SprB ensure the gene rearrangement and stable expression of spsM during sporulation in Bacillus subtilis

    doi: 10.1093/nar/gkx466

    Figure Lengend Snippet: DNA cleavage and strand exchange by SprA. ( A ) Direct sequencing of the intermediates of the SprA-mediated integration reaction. The intermediates: the left and right half-sites of attP , were directly sequenced using a BigDye Terminator v3.1 Cycle Sequencing Kit and an ABI 3500 DNA analyzer. The 16 bp overlapping sequence is underlined. The 3΄-end nucleotides indicated by the circles are adenines added by the terminal transferase activity of Taq polymerase used for the cycle sequencing reaction. The cleavage point of attP is shown to the left (indicated by the lines and triangles). ( B ) Effects of point mutations of the central dinucleotides on DNA recombination. Point mutations were introduced into the AAA nucleotides at the center of the attB site (A→T). In vitro integration recombination was performed using 0.5 μM SprA and 50 ng each of the wild-type attP and the mutated attB substrates.

    Article Snippet: DNA pellets were dissolved in TE buffer, gel purified, and directly sequenced using the P107 (for the left half- site of attP ) or P109 (for the right half- site) primers, a BigDye® Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific, WI, USA) and an ABI 3500 DNA analyzer (Thermo Fisher Scientific).

    Techniques: Sequencing, Activity Assay, In Vitro

    The DNA sequencing of rs840088. Direct sequencing of a subgroup of samples with the same primers for PCR-RFLP was performed using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, USA). (A) GG genotype. (B) GA genotype. (C) AA genotype. 82×48 mm (300×300 DPI).

    Journal: Yonsei Medical Journal

    Article Title: The Association of SERPINE2 Gene with COPD in a Chinese Han Population

    doi: 10.3349/ymj.2011.52.6.953

    Figure Lengend Snippet: The DNA sequencing of rs840088. Direct sequencing of a subgroup of samples with the same primers for PCR-RFLP was performed using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, USA). (A) GG genotype. (B) GA genotype. (C) AA genotype. 82×48 mm (300×300 DPI).

    Article Snippet: Sequencing was performed using the BigDye Terminator v1.1 Cycle Sequencing kit (Applied Biosystems), and analyzed on an ABI PRISM 3100 DNA sequencer (Applied Biosystems, Foster City, CA, USA).

    Techniques: DNA Sequencing, Sequencing, Polymerase Chain Reaction

    CAST promoter activity and calpastatin isoform expression in MG cells. A , RLM RACE was carried out using 5 μg of total RNA isolated from NFI-hyperphosphorylated (T98) or NFI-hypophosphorylated (U251) MG cells. Antisense primer targeting exon 14 of the CAST gene was used for reverse transcription. Nested PCR amplification was carried out with 5′-ligated outer and inner primers and antisense primers targeting exons 8 and 4 of the CAST gene, respectively. PCR products were electrophoresed in 3% Metaphor (FMC Bioproducts) agarose gel, visualized with ethidium bromide, excised, purified, and sequenced using the BigDye Terminator version 3.1 cycle sequencing kit (Thermo Fisher Scientific). Exon composition of the isolated DNA products based on sequence analysis is indicated on the right. B , domain composition of XL-containing (full-length/type II) and XL-less (type III) calpastatin isoforms. T98 ( C ) and U251 ( D ) MG cells were transiently transfected with luciferase reporter constructs containing the CP (∼2000 bp upstream of CAST exon 1), ALT (in intron 3 containing the NFI-binding sites; ∼4000 bp upstream of CAST exon 4), or empty vector (CNT). Luciferase activity was measured using the Luciferase Assay System (Promega) and the FLUOstar microplate reader (BMG LABTECH). Relative -fold change was calculated relative to the empty vector control. Scatter plots show data from three independent experiments. E , U251 MG cells were transiently transfected with luciferase reporter constructs containing the WT ALT or constructs containing mutation at C2 (ALT-C2*), C3 (ALt-C3*), or both C2 and C3 (ALT-C2*C3*) NFI-binding sites. Luciferase activity was measured 48 h post-transfection as described above. p values were obtained from one-way analysis of variance statistical analysis of three independent experiments. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Effects of nuclear factor I phosphorylation on calpastatin (CAST) gene variant expression and subcellular distribution in malignant glioma cells

    doi: 10.1074/jbc.RA118.004787

    Figure Lengend Snippet: CAST promoter activity and calpastatin isoform expression in MG cells. A , RLM RACE was carried out using 5 μg of total RNA isolated from NFI-hyperphosphorylated (T98) or NFI-hypophosphorylated (U251) MG cells. Antisense primer targeting exon 14 of the CAST gene was used for reverse transcription. Nested PCR amplification was carried out with 5′-ligated outer and inner primers and antisense primers targeting exons 8 and 4 of the CAST gene, respectively. PCR products were electrophoresed in 3% Metaphor (FMC Bioproducts) agarose gel, visualized with ethidium bromide, excised, purified, and sequenced using the BigDye Terminator version 3.1 cycle sequencing kit (Thermo Fisher Scientific). Exon composition of the isolated DNA products based on sequence analysis is indicated on the right. B , domain composition of XL-containing (full-length/type II) and XL-less (type III) calpastatin isoforms. T98 ( C ) and U251 ( D ) MG cells were transiently transfected with luciferase reporter constructs containing the CP (∼2000 bp upstream of CAST exon 1), ALT (in intron 3 containing the NFI-binding sites; ∼4000 bp upstream of CAST exon 4), or empty vector (CNT). Luciferase activity was measured using the Luciferase Assay System (Promega) and the FLUOstar microplate reader (BMG LABTECH). Relative -fold change was calculated relative to the empty vector control. Scatter plots show data from three independent experiments. E , U251 MG cells were transiently transfected with luciferase reporter constructs containing the WT ALT or constructs containing mutation at C2 (ALT-C2*), C3 (ALt-C3*), or both C2 and C3 (ALT-C2*C3*) NFI-binding sites. Luciferase activity was measured 48 h post-transfection as described above. p values were obtained from one-way analysis of variance statistical analysis of three independent experiments. *, p

    Article Snippet: The DNA was electrophoresed in a 3% MetaPhor agarose (FMC Bioproducts, Rockland, ME) gel, excised, purified (Monarch DNA extraction kit, New England Biolabs), and sequenced (BigDye Terminator® version 3.1 cycle sequencing kit, Thermo Fisher Scientific).

    Techniques: Activity Assay, Expressing, Isolation, Nested PCR, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Purification, Sequencing, Transfection, Luciferase, Construct, Binding Assay, Plasmid Preparation, Mutagenesis