Article Title: Inhibition of Rabies Virus by 1,2,3,4,6-Penta-O-galloyl-β-d-Glucose Involves mTOR-Dependent Autophagy
Figure Lengend Snippet: PGG inhibits the replication of RABV via mTOR-associated autophagy. ( a ) mTOR pathway-associated proteins and autophagy markers were analyzed by Western blotting, after CVS-infected BHK-21 cells treated with PGG for 24 h. ( b ) The relative expression levels of mTOR, p-mTOR, SQSTM1, LC3, and viral P protein, normalizing to that of α-tubulin. BHK-21 cells were pretreated with rapamycin (1 or 2 μM) or MHY1485 (5 or 10 μM) for 1 h, then infected with CVS-11 and cultured in medium containing rapamycin or MHY1485 for 24 h. ( c , e ) p-mTOR, autophagy markers, and P protein were analyzed by Western blotting. ( d , f ) The relative expression levels of p-mTOR, SQSTM1, LC3, and viral P, normalizing to that of α-tubulin. (* p
Article Snippet: BHK-21 cells were propagated in Minimum Essential Medium (MEM; Corning Inc., Corning, NY, USA) supplemented with 5% fetal bovine serum (FBS; Corning), 100 U/mL penicillin G, and 100 µg/mL streptomycin.
Techniques: Western Blot, Infection, Expressing, Cell Culture