Journal: International Journal of Molecular Sciences
Article Title: Identification of the Avian Pasteurella multocida phoP Gene and Evaluation of the Effects of phoP Deletion on Virulence and Immunogenicity
Figure Lengend Snippet: Phenotype characterization of the P. multocida Δ phoP mutant. ( A ) The growth curve of the parent strain and the Δ phoP mutant. P. multocida were cultured in brain heart infusion (BHI) broth supplemented with or without kanamycin, and then the OD 600 value of each strain was measured every 2 h over a period of 14 h; ( B , C ) The OMP and LPS profiles of P. multocida . OMPs or LPS were extracted from the parent strain or the Δ phoP mutant and were then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Next, Coomassie blue staining and silver staining were applied to visualize the OMPs ( B ) and LPS ( C ), respectively. M refers to the protein Marker; ( D ) The resistance to polymyxin B. The parent strain, S413 and the complement stain S413 (pQK180) were cultured in BHI broth with or without 0.5 μg/mL polymyxin B for 1 h, and the survival ratio of each strain was calculated as the mean CFU of the polymyxin B-treated group divided by the mean CFU of the untreated group. The data in D are expressed as the mean ± SD and were analyzed at the significance level of 0.05 (*).
Article Snippet: The P. multocida strains were grown in BHI broth (BD Bioscience, San Jose, CA, USA).
Techniques: Mutagenesis, Cell Culture, Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Silver Staining, Marker