Journal: Stem cell research

Article Title: Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla.

doi: 10.1016/j.scr.2011.12.005

Figure Lengend Snippet: Figure 5 Effect of IGF-1 on osteo/odontogenic gene expression of SCAPs. A: QRT-PCR results of gene expression in untreated and trea- ted samples for ALP, RUNX2, OSX, OCN and DSPP. GAPDH was used as an internal control. Gene expression was described as a fold change relative to the control group. Values are the means±SD n=4 (**2−ΔΔCt≥2, Pb0.01; *1b2−ΔΔCtb2, Pb0.01). B: Western blot results of pro- tein levels in untreated and treated SCAPs for RUNX2, OSX, OCN and DSP. β-ACTIN was used as a loading control.

Article Snippet: Membranes were blocked at room temperature for 2 h with blocking solution (5% w/v skim milk, 0.01 mol/L PBS, 0.1% Tween-20), and subsequently incubated overnight at 4 °C with primary polyclonal antibodies against RUNX2 (1:300; BOSTER, China), DSP (1:500; Santa Cruz), OSX (1:300; BOSTER, China), OCN (1:300; BOSTER, China) and monoclonal antibody against βACTIN (1:1,000; ABGENT, Flanders Count, CA, USA).

Techniques: Gene Expression, Quantitative RT-PCR, Control, Western Blot

Journal: Stem cell research

Article Title: Insulin-like growth factor 1 can promote the osteogenic differentiation and osteogenesis of stem cells from apical papilla.

doi: 10.1016/j.scr.2011.12.005

Figure Lengend Snippet: Figure 7 Immunohistological staining of osteo/odontogenic markers in IGF-1 treated SCAPs in vivo. A: Immunohistological positive staining for DSP in odontoblast-like cells, predentin, and dentin structures of untreated SCAPs pellets. B: Negative staining for OSX in odontoblastic layer and dentin of untreated SCAPs pellets. C: Positive staining for OCN in odontoblast-like cells and weaker positive in osteodentin-like tissues of untreated SCAPs pellets. Negative staining for OCN appeared in dentin layer. D: Negative control using PBS instead of primary antibodies in untreated SCAPs pellets. E: Weaker positive staining for DSP in bone-like structures generated by IGF- 1 treated SCAPs. F: Positive staining for OSX in osteoblast-like cells of IGF-1 treated SCAPs pellets. G: Positive staining for OCN in bone-like structures generated by IGF-1 treated SCAPs. H: Negative control using PBS instead of primary antibodies in IGF-1 treated SCAPs implants. Scale bars=50 μm. Abbreviations: B, bone-like tissue; D, dentin; OD, osteodentin-like tissues; ODB, odontoblast-like cell; PD, predentin.

Article Snippet: Membranes were blocked at room temperature for 2 h with blocking solution (5% w/v skim milk, 0.01 mol/L PBS, 0.1% Tween-20), and subsequently incubated overnight at 4 °C with primary polyclonal antibodies against RUNX2 (1:300; BOSTER, China), DSP (1:500; Santa Cruz), OSX (1:300; BOSTER, China), OCN (1:300; BOSTER, China) and monoclonal antibody against βACTIN (1:1,000; ABGENT, Flanders Count, CA, USA).

Techniques: Staining, In Vivo, Negative Staining, Negative Control, Generated

Journal: Journal of the mechanical behavior of biomedical materials

Article Title: Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells

doi: 10.1016/j.jmbbm.2019.03.017

Figure Lengend Snippet: Primers used for TAQMAN gene expression analyses.

Article Snippet: BGLAP , Ss03373655_s1.

Techniques: Gene Expression

Journal: Journal of the mechanical behavior of biomedical materials

Article Title: Shape-fitting collagen-PLA composite promotes osteogenic differentiation of porcine adipose stem cells

doi: 10.1016/j.jmbbm.2019.03.017

Figure Lengend Snippet: Gene expression profiles of RUNX2, BMP2, BGLAP, Osterix, COL1A2, and MMP9 for pASCs within the mineralized collagen scaffold vs. collagen-PLA composite. * indicates significant (p < 0.05) higher gene expression between the two groups on the same day. ^ indicates significantly (p < 0.05) higher gene expression of one group compared to the same group on day 1. # indicates significantly (p < 0.05) lower gene expression of one group compared to the same group on day 1. Data expressed as mean ± standard deviation (n=5).

Article Snippet: BGLAP , Ss03373655_s1.

Techniques: Gene Expression, Standard Deviation

Journal: The Kaohsiung journal of medical sciences

Article Title: Morinda officinalis polysaccharide regulates rat bone mesenchymal stem cell osteogenic-adipogenic differentiation in osteoporosis by upregulating miR-21 and activating the PI3K/AKT pathway.

doi: 10.1002/kjm2.12544

Figure Lengend Snippet: FIGURE 6 MOP improved OP in OVX rats and increased the expression of miR-21. OVX OP model rats were treated with MOP for 4 weeks. (A) The BMD of the isolated lumbar spine was measured by an X-ray BMD detector; (B,C) the levels of BALP and BGP in serum were detected by ELISA; and (D) the expression of miR-21 in rat BMSCs was detected by RT-qPCR. The data were expressed as mean ± standard deviation. Animal experiment N = 6. One-way ANOVA was adopted for data comparisons among groups, followed by Tukey's multiple comparisons test. p value was obtained by a bilateral test, **p < 0.01 and ***p < 0.001. BGP, osteocalcin; BMD, bone mineral density; BALP, bone-derived alkaline phosphatase; BMSC, bone mesenchymal stem cell; MOP, Morinda officinalis polysaccharide; OP, osteoporosis; OVX, ovariectomy

Article Snippet: The levels of serum bone alkaline phosphatase (BALP, CSB-E11188r, CUSABIO, Wuhan, Hubei, China) and osteocalcin (BGP, CSBE05129r, CUSABIO) were detected using ELISA kits in strict accordance with the instructions.

Techniques: Expressing, Isolation, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Standard Deviation, Derivative Assay