bglii Takara Search Results


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  • 90
    TaKaRa bglii
    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and <t>BglII,</t> cloned into the <t>pMD18-T</t> vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp
    Bglii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 2110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bglii - by Bioz Stars, 2020-02
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    78
    TaKaRa bglii ecori digested pmscv
    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and <t>BglII,</t> cloned into the <t>pMD18-T</t> vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp
    Bglii Ecori Digested Pmscv, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    TaKaRa bglii ecori digested pegfp c1
    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and <t>BglII,</t> cloned into the <t>pMD18-T</t> vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp
    Bglii Ecori Digested Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii ecori digested pegfp c2
    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and <t>BglII,</t> cloned into the <t>pMD18-T</t> vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp
    Bglii Ecori Digested Pegfp C2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bgli  (TaKaRa)
    82
    TaKaRa bgli
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bgli, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pcmv ha
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Pcmv Ha, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    TaKaRa bglii sali digested pegfp c1
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Sali Digested Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii hindiii digested pegfp c1
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Hindiii Digested Pegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    TaKaRa bglii sali digested mcherry c1
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Sali Digested Mcherry C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    TaKaRa sali bglii digested pcmv myc
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Sali Bglii Digested Pcmv Myc, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii restriction site
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Restriction Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa bglii sali
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Sali, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    TaKaRa bglii ecori res triction endonuclease
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Ecori Res Triction Endonuclease, supplied by TaKaRa, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa restriction enzymes
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Restriction Enzymes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii acci
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Acci, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TaKaRa bglii ecori sites
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Ecori Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    TaKaRa pegfp n3 bglii sali site
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Pegfp N3 Bglii Sali Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    TaKaRa bglii site
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa bglii smai insertion
    Restriction patterns of each genome type based on five restriction endonucleases: <t>BglI</t> (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log <t>DNA</t> ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative
    Bglii Smai Insertion, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa bglii sali site
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Sali Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii sali digested vector pecfp c1
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Sali Digested Vector Pecfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa pmscv puro clontech bglii
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Pmscv Puro Clontech Bglii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa restriction enzyme bglii
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Restriction Enzyme Bglii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii bamhi digested pecfp c1
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Bamhi Digested Pecfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    TaKaRa hindiii bglii
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Hindiii Bglii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii sali cut plegfp c1
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Sali Cut Plegfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii ecori digested vector pegfp n1
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Ecori Digested Vector Pegfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    TaKaRa bglii sali digested pegfp c1 vector
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Sali Digested Pegfp C1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii digested pecfp c1
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Digested Pecfp C1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    TaKaRa bglii hindiii cut plpcx
    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the <t>BglII</t> and <t>SalI</t> sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P
    Bglii Hindiii Cut Plpcx, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pcmv ha vector
    Expression of wt, but not that of catalytically inactive <t>mPPM1G,</t> can rescue correct nuclear localization of the SMN complex in CBs. HeLa cells were first transfected with siRNA oligonucleotide #1 against PPM1G mRNA or a control siRNA oligo (c). Next, cells were transfected with <t>pCMV-HA</t> vectors encoding either wt (HA-mPPM1Gwt or mwt) or phosphatase-dead (HA-mPPM1G-D493A or mD493A) mPPM1G, 12 h after the first transfection. 48 h after the first transfection, cells were fixed and immunostained with antibodies against SMN and the HA tag of mPPM1G. (A) Representative images of cells showing SMN, HA, and DAPI after knockdown of endogenous PPM1G and overexpression of mPPM1G versions as indicated. Bars, 20 μm. (B) Quantification of phenotypes. The relative number of cells showing localization of SMN protein in CBs is plotted. Numbers are means from three independent experiments and error bars represent SD. Absolute numbers of counted cells (1/2/3 experiment): control, 104/171/197; ΔhPPM1G, 139/180/187; ΔhPPM1G + mwt, 67/164/160; and ΔhPPM1G + mD496A, 75/160/171.
    Pcmv Ha Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    TaKaRa ecori bglii pcmv ha vector
    Expression of wt, but not that of catalytically inactive <t>mPPM1G,</t> can rescue correct nuclear localization of the SMN complex in CBs. HeLa cells were first transfected with siRNA oligonucleotide #1 against PPM1G mRNA or a control siRNA oligo (c). Next, cells were transfected with <t>pCMV-HA</t> vectors encoding either wt (HA-mPPM1Gwt or mwt) or phosphatase-dead (HA-mPPM1G-D493A or mD493A) mPPM1G, 12 h after the first transfection. 48 h after the first transfection, cells were fixed and immunostained with antibodies against SMN and the HA tag of mPPM1G. (A) Representative images of cells showing SMN, HA, and DAPI after knockdown of endogenous PPM1G and overexpression of mPPM1G versions as indicated. Bars, 20 μm. (B) Quantification of phenotypes. The relative number of cells showing localization of SMN protein in CBs is plotted. Numbers are means from three independent experiments and error bars represent SD. Absolute numbers of counted cells (1/2/3 experiment): control, 104/171/197; ΔhPPM1G, 139/180/187; ΔhPPM1G + mwt, 67/164/160; and ΔhPPM1G + mD496A, 75/160/171.
    Ecori Bglii Pcmv Ha Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and BglII, cloned into the pMD18-T vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp

    Journal: Applied and Environmental Microbiology

    Article Title: Chromosomal Insertions in the Lactobacillus caseiupp Gene That Are Useful for Vaccine Expression

    doi: 10.1128/AEM.00175-14

    Figure Lengend Snippet: Schematic diagram of vector construction. 1, PCR products of upstream homologous arm 2A with AscI and BglII, cloned into the pMD18-T vector and named pMD18-T-2A. pMD18-T-2A was digested with AscI and BglII endonucleases and inserted into the pGBHCupp

    Article Snippet: The pMD18-T-2A plasmid was digested with BglII (TaKaRa) and AscI (NEB).

    Techniques: Plasmid Preparation, Polymerase Chain Reaction, Clone Assay

    Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.

    Journal: Emerging Microbes & Infections

    Article Title: Comparative genomic analysis of two emergent human adenovirus type 14 respiratory pathogen isolates in China reveals similar yet divergent genomes

    doi: 10.1038/emi.2017.78

    Figure Lengend Snippet: Identification of the genome types of HAdV-B14 strains GZ01 (Guangzhou), BJ430 (Beijing), and CHN2012 (Beijing). The in silico restriction enzyme analysis (REA) digestion patterns of HAdV-14 genomes were generated using the Vector NTI 10.3.0 software (Invitrogen Corp., San Diego, CA, USA). Genome digestion patterns of the strains are as follows: ( A ) de Wit or prototype (AY803294); ( B ) GZ01 (JQ824845); ( C ) 303600, reported as HAdV-B14p1 (FJ822614); ( D ) BJ430 (JN032132); and ( E ) CHN2012 (JX892927). These were analyzed with Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal I, Sma I, Xba I, and Xho I, as described by Li et al. 59 All the three recent China outbreak genomes correspond to the genome type of HAdV-14p1 and have identical REA profiles to each other. They are divergent from the HAdV-B14 prototype (1955). M: 1 kb DNA Sizing Ladder.

    Article Snippet: Genome type identification In silico restriction maps of available genome sequences from all HAdV-14 strains CHN/GZ01/2010, 303600, CHN/BJ430, CHN2012, and prototype de Wit were generated using the Vector NTI 10.3.0 software (Invitrogen Corp.; San Diego, CA, USA) as described in earlier studies., , These included profiles from restriction enzymes Bam HI, Bcl I, Bgl l, Bgl II, Bst EII, Eco RI, Hin dIII, Hpa I, Sal l, Sma I, Xba I, and Xho I (TaKaRa Corp.; China), all of which were chosen in order to be consistent with the original nomenclature system devised by Li and colleagues.

    Techniques: In Silico, Generated, Plasmid Preparation, Software

    In vitro MMR efficiency of BS cell lines. ( A ) Western blot showing the absence of BLM protein in the BS cell lines. Aliquots of 25 µg of the indicated nuclear extracts were probed with anti-BLM antibody (IHIC33) and anti-XPB antibody [TFIIH p89 (S-19); Santa Cruz Biotechnology] as control. ( B ) MMR efficiency of the BLM-negative human fibroblast GM08505 and of the human lymphoblasts GM09960 and GM03403. The MMR-proficient MRC5 SV40 fibroblasts, TK6 lymphoblasts, PSNF5 cells (GM08505 stably transfected with BLM cDNA) and the MMR-deficient hMLH1 –/– colon cancer cell line HCT116 were used as controls. The MMR efficiencies of the two BS lymphoblasts complemented with purified recombinant BLM protein are also shown. The repair efficiency is expressed as fmol phagemid DNA cleaved by Bgl II in 30 min.

    Journal: Nucleic Acids Research

    Article Title: Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1

    doi:

    Figure Lengend Snippet: In vitro MMR efficiency of BS cell lines. ( A ) Western blot showing the absence of BLM protein in the BS cell lines. Aliquots of 25 µg of the indicated nuclear extracts were probed with anti-BLM antibody (IHIC33) and anti-XPB antibody [TFIIH p89 (S-19); Santa Cruz Biotechnology] as control. ( B ) MMR efficiency of the BLM-negative human fibroblast GM08505 and of the human lymphoblasts GM09960 and GM03403. The MMR-proficient MRC5 SV40 fibroblasts, TK6 lymphoblasts, PSNF5 cells (GM08505 stably transfected with BLM cDNA) and the MMR-deficient hMLH1 –/– colon cancer cell line HCT116 were used as controls. The MMR efficiencies of the two BS lymphoblasts complemented with purified recombinant BLM protein are also shown. The repair efficiency is expressed as fmol phagemid DNA cleaved by Bgl II in 30 min.

    Article Snippet: The yeast strain L40 [ MATa trp1 leu2 his3 LYS2 :: lexA-HIS3 URA3 :: lexA-lacZ ] was sequentially transformed with the bait pBTM116-BLM (amino acids 770–1417) and a random primed human peripheral blood cDNA library cloned into the Bgl II sites of pACT (Clonetech) using the lithium acetate method.

    Techniques: In Vitro, Western Blot, Stable Transfection, Transfection, Purification, Recombinant

    Double-enzyme digestion of pAdTrack-CMV-hHSP27. M1: DL2000 Marker; 1: Sal I and Bgl II enzyme digestion results of pAdTrack-CMV-hHSP27; M2: Hind III enzyme digestionλ-DNA Marker; 2: PCR products of full-length human HSP27.

    Journal: PLoS ONE

    Article Title: Effects of Upregulation of Hsp27 Expression on Oocyte Development and Maturation Derived from Polycystic Ovary Syndrome

    doi: 10.1371/journal.pone.0083402

    Figure Lengend Snippet: Double-enzyme digestion of pAdTrack-CMV-hHSP27. M1: DL2000 Marker; 1: Sal I and Bgl II enzyme digestion results of pAdTrack-CMV-hHSP27; M2: Hind III enzyme digestionλ-DNA Marker; 2: PCR products of full-length human HSP27.

    Article Snippet: The recombinant adenovirus shuttle plasmid pAdTrack-CMV-hHsp27 was first digested by Sal I and Bgl II enzyme (Takara Shuzo Co. Ltd. Kyoto, Japan), then measured in 0.8% agar gel electrophoresis.

    Techniques: Marker, Polymerase Chain Reaction

    Restriction patterns of each genome type based on five restriction endonucleases: BglI (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log DNA ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative

    Journal: Journal of Clinical Microbiology

    Article Title: Five New Genome Types of Adenovirus Type 37 Caused Epidemic Keratoconjunctivitis in Sapporo, Japan, for More Than 10 Years

    doi: 10.1128/JCM.43.2.726-732.2005

    Figure Lengend Snippet: Restriction patterns of each genome type based on five restriction endonucleases: BglI (A), EcoRI (B), SacI (C), SmaI (D), and XhoI (E). Lane m, 2-log DNA ladder marker (New England Biolabs); lane p, adenovirus type 37 prototype; lanes a to h, representative

    Article Snippet: Aliquots of 1 μg of viral DNA were digested with 5 U of the following restriction endonucleases: BamHI, BglI, BglII, EcoRI, HindIII, SacI, SmaI, and XhoI (Takara Shuzo, Kyoto, Japan).

    Techniques: Marker

    Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the BglII and SalI sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P

    Journal: Nucleic Acids Research

    Article Title: MBNL and CELF proteins regulate alternative splicing of the skeletal muscle chloride channel CLCN1

    doi: 10.1093/nar/gkp681

    Figure Lengend Snippet: Splicing regulation of MBNL and CELF proteins. ( A ) Structure of chloride channel minigenes. Both human CLCN1/ClC-1 and mouse Clcn1 minigenes were subcloned between the BglII and SalI sites of pEGFP-C1. Black boxes represent exons of the minigenes. Arrows indicate the position of primers used in the splicing assays. Exon 6B is a human-specific exon and is absent in Clcn1 . ( B ) Splicing regulation of Clcn1 by MBNL and CELF proteins. Representative results of cellular splicing assays using the Clcn1 minigene in COS-7 cells. The upper bands correspond to a splice product containing exon 7A, whereas lower bands correspond to a splice product lacking exon 7A. Bar chart shows quantified results of exon 7A inclusion (mean ± SD, n = 3). Statistical significance was analyzed by analysis of variance (ANOVA) and Dunnett's multiple comparisons. All MBNL proteins and CELF proteins except for CUG-BP and ETR-3 showed significant differences (* P

    Article Snippet: The minigene fragments in pGEM-T Easy were cleaved by BamHI and SalI and then subcloned into the BglII-SalI site of pEGFP-C1 (Clontech).

    Techniques:

    Expression of wt, but not that of catalytically inactive mPPM1G, can rescue correct nuclear localization of the SMN complex in CBs. HeLa cells were first transfected with siRNA oligonucleotide #1 against PPM1G mRNA or a control siRNA oligo (c). Next, cells were transfected with pCMV-HA vectors encoding either wt (HA-mPPM1Gwt or mwt) or phosphatase-dead (HA-mPPM1G-D493A or mD493A) mPPM1G, 12 h after the first transfection. 48 h after the first transfection, cells were fixed and immunostained with antibodies against SMN and the HA tag of mPPM1G. (A) Representative images of cells showing SMN, HA, and DAPI after knockdown of endogenous PPM1G and overexpression of mPPM1G versions as indicated. Bars, 20 μm. (B) Quantification of phenotypes. The relative number of cells showing localization of SMN protein in CBs is plotted. Numbers are means from three independent experiments and error bars represent SD. Absolute numbers of counted cells (1/2/3 experiment): control, 104/171/197; ΔhPPM1G, 139/180/187; ΔhPPM1G + mwt, 67/164/160; and ΔhPPM1G + mD496A, 75/160/171.

    Journal: The Journal of Cell Biology

    Article Title: Dephosphorylation of survival motor neurons (SMN) by PPM1G/PP2C? governs Cajal body localization and stability of the SMN complex

    doi: 10.1083/jcb.200704163

    Figure Lengend Snippet: Expression of wt, but not that of catalytically inactive mPPM1G, can rescue correct nuclear localization of the SMN complex in CBs. HeLa cells were first transfected with siRNA oligonucleotide #1 against PPM1G mRNA or a control siRNA oligo (c). Next, cells were transfected with pCMV-HA vectors encoding either wt (HA-mPPM1Gwt or mwt) or phosphatase-dead (HA-mPPM1G-D493A or mD493A) mPPM1G, 12 h after the first transfection. 48 h after the first transfection, cells were fixed and immunostained with antibodies against SMN and the HA tag of mPPM1G. (A) Representative images of cells showing SMN, HA, and DAPI after knockdown of endogenous PPM1G and overexpression of mPPM1G versions as indicated. Bars, 20 μm. (B) Quantification of phenotypes. The relative number of cells showing localization of SMN protein in CBs is plotted. Numbers are means from three independent experiments and error bars represent SD. Absolute numbers of counted cells (1/2/3 experiment): control, 104/171/197; ΔhPPM1G, 139/180/187; ΔhPPM1G + mwt, 67/164/160; and ΔhPPM1G + mD496A, 75/160/171.

    Article Snippet: For the HA-mPPM1G construct, mPPM1G was amplified from IRAKp961L079Q (RZPD) and cloned into the pCMV-HA vector (SalI–BglII; CLONTECH Laboratories, Inc.).

    Techniques: Expressing, Transfection, Over Expression