bglii Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs bglii
    Confirmation of chromatin looping by 3C assay with <t>BglII</t> and/or <t>ApaLI</t> restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII
    Bglii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2496 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/New England Biolabs
    Average 99 stars, based on 2496 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher bglii
    <t>DNA</t> inversion in the UU172 element of U. parvum serotype 3 clonal variants 27815 T -K5 and M14-K16. Schematic illustration of the DNA inversion event in (A) 27815 T -K5 and (B) M14-K16. Southern blot analyses of (A′) 27815 T -K5 before (P0) and after (P3) selective pressure with Pab α-U172C and (B′) M14-K16 before and after selective pressure with Pab α-U144. Genomic DNA was digested with <t>BglII</t> and HincII and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots.
    Bglii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Thermo Fisher
    Average 97 stars, based on 1658 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    97/100 stars
      Buy from Supplier

    94
    Millipore bglii
    <t>DNA</t> inversion in the UU172 element of U. parvum serotype 3 clonal variants 27815 T -K5 and M14-K16. Schematic illustration of the DNA inversion event in (A) 27815 T -K5 and (B) M14-K16. Southern blot analyses of (A′) 27815 T -K5 before (P0) and after (P3) selective pressure with Pab α-U172C and (B′) M14-K16 before and after selective pressure with Pab α-U144. Genomic DNA was digested with <t>BglII</t> and HincII and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots.
    Bglii, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Millipore
    Average 94 stars, based on 459 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher fastdigest bglii
    <t>DNA</t> inversion in the UU172 element of U. parvum serotype 3 clonal variants 27815 T -K5 and M14-K16. Schematic illustration of the DNA inversion event in (A) 27815 T -K5 and (B) M14-K16. Southern blot analyses of (A′) 27815 T -K5 before (P0) and after (P3) selective pressure with Pab α-U172C and (B′) M14-K16 before and after selective pressure with Pab α-U144. Genomic DNA was digested with <t>BglII</t> and HincII and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots.
    Fastdigest Bglii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest bglii/product/Thermo Fisher
    Average 93 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    fastdigest bglii - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    93
    GenScript bglii
    <t>DNA</t> inversion in the UU172 element of U. parvum serotype 3 clonal variants 27815 T -K5 and M14-K16. Schematic illustration of the DNA inversion event in (A) 27815 T -K5 and (B) M14-K16. Southern blot analyses of (A′) 27815 T -K5 before (P0) and after (P3) selective pressure with Pab α-U172C and (B′) M14-K16 before and after selective pressure with Pab α-U144. Genomic DNA was digested with <t>BglII</t> and HincII and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots.
    Bglii, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/GenScript
    Average 93 stars, based on 104 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    94
    Promega bglii
    <t>DNA</t> inversion in the UU172 element of U. parvum serotype 3 clonal variants 27815 T -K5 and M14-K16. Schematic illustration of the DNA inversion event in (A) 27815 T -K5 and (B) M14-K16. Southern blot analyses of (A′) 27815 T -K5 before (P0) and after (P3) selective pressure with Pab α-U172C and (B′) M14-K16 before and after selective pressure with Pab α-U144. Genomic DNA was digested with <t>BglII</t> and HincII and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots.
    Bglii, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Promega
    Average 94 stars, based on 1418 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    bglii  (Roche)
    92
    Roche bglii
    Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. <t>pTW423</t> is digested with <t>BglII</t> and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.
    Bglii, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Roche
    Average 92 stars, based on 174 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    99
    TaKaRa bglii
    Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by <t>KpnI</t> and <t>BglII</t> (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.
    Bglii, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1871 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/TaKaRa
    Average 99 stars, based on 1871 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    92
    OriGene bglii
    Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by <t>KpnI</t> and <t>BglII</t> (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.
    Bglii, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/OriGene
    Average 92 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Evrogen bglii
    Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by <t>KpnI</t> and <t>BglII</t> (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.
    Bglii, supplied by Evrogen, used in various techniques. Bioz Stars score: 92/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Evrogen
    Average 92 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Kaneka Corp bglii
    Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by <t>KpnI</t> and <t>BglII</t> (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.
    Bglii, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bglii/product/Kaneka Corp
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bglii - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Confirmation of chromatin looping by 3C assay with BglII and/or ApaLI restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII

    Journal: The Journal of Biological Chemistry

    Article Title: Sp1 Regulates Chromatin Looping between an Intronic Enhancer and Distal Promoter of the Human Heme Oxygenase-1 Gene in Renal Cells *

    doi: 10.1074/jbc.M109.058586

    Figure Lengend Snippet: Confirmation of chromatin looping by 3C assay with BglII and/or ApaLI restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII

    Article Snippet: SDS was then sequestered from the samples by the addition of 1.8% Triton X-100 (Fisher), and the samples were incubated at 37 °C for 1 h. The samples were digested with BglII and/or ApaLI restriction enzyme (New England Biolabs) at 37 °C for 16 h. Restriction enzymes were inactivated by the addition of 1.6% SDS and further incubation at 65 °C for 20 min.

    Techniques:

    Maps of pNZC and inserts used in this study. A chloride-inducible promoter (CIP) was inserted between BglII and NcoI. P gadR is a constitutive promoter controlling the production of the activator protein GadR. lacZ and AMP expression are controlled by the chloride-inducible promoter P gad (activated by GadR). lacZ and Bac are inserted between cut sites NcoI and SpeI in pNZC to create pNZCL and pNZCA3.

    Journal: Applied and Environmental Microbiology

    Article Title: Chloride-Inducible Expression Vector for Delivery of Antimicrobial Peptides Targeting Antibiotic-Resistant Enterococcus faecium

    doi: 10.1128/AEM.00227-15

    Figure Lengend Snippet: Maps of pNZC and inserts used in this study. A chloride-inducible promoter (CIP) was inserted between BglII and NcoI. P gadR is a constitutive promoter controlling the production of the activator protein GadR. lacZ and AMP expression are controlled by the chloride-inducible promoter P gad (activated by GadR). lacZ and Bac are inserted between cut sites NcoI and SpeI in pNZC to create pNZCL and pNZCA3.

    Article Snippet: The PCR fragment and plasmid pNZ8048 were both digested with restriction enzymes BglII and NcoI (New England BioLabs) and ligated at 16°C for 16 h. The resulting ligation was then transformed into electrocompetent E. coli MC1061 F′ (Lucigen).

    Techniques: Expressing, BAC Assay

    Variability of Cre Expression of Mouse Line Tg Cre4 (A) Variable Cre expression in forebrains of three different mice positive for Tg Cre4 and R26R Cre indicator (see Figure S1 ) at postnatal day 12 pictured by the Cre-dependent β-galactosidase activity (blue, X-gal, counterstain by eosin) in coronal brain slices. Scale bar: 1.25 mm. (B) Southern blot analysis of BglII-digested genomic mouse DNA of four Tg Cre4 mice that differed in the Cre expression pattern. Southern probe detects the wild-type (4.5 kbp) and the transgenic (7, 5, 3, and 2 kbp) alleles.

    Journal: PLoS Biology

    Article Title: Enhanced Odor Discrimination and Impaired Olfactory Memory by Spatially Controlled Switch of AMPA ReceptorsGenetic and Behavioral Investigations into Odor Discrimination and Memory

    doi: 10.1371/journal.pbio.0030354

    Figure Lengend Snippet: Variability of Cre Expression of Mouse Line Tg Cre4 (A) Variable Cre expression in forebrains of three different mice positive for Tg Cre4 and R26R Cre indicator (see Figure S1 ) at postnatal day 12 pictured by the Cre-dependent β-galactosidase activity (blue, X-gal, counterstain by eosin) in coronal brain slices. Scale bar: 1.25 mm. (B) Southern blot analysis of BglII-digested genomic mouse DNA of four Tg Cre4 mice that differed in the Cre expression pattern. Southern probe detects the wild-type (4.5 kbp) and the transgenic (7, 5, 3, and 2 kbp) alleles.

    Article Snippet: Southern blot analysis Genomic DNA from mouse-tail/liver was digested with restriction enzyme BglII (NEB), and the Southern blot was done with a 320-bp probe (“integ”) obtained by PCR detecting the αCaMKII promoter.

    Techniques: Expressing, Mouse Assay, Activity Assay, Southern Blot, Transgenic Assay

    DNA inversion in the UU172 element of U. parvum serotype 3 clonal variants 27815 T -K5 and M14-K16. Schematic illustration of the DNA inversion event in (A) 27815 T -K5 and (B) M14-K16. Southern blot analyses of (A′) 27815 T -K5 before (P0) and after (P3) selective pressure with Pab α-U172C and (B′) M14-K16 before and after selective pressure with Pab α-U144. Genomic DNA was digested with BglII and HincII and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots.

    Journal: Molecular Microbiology

    Article Title: Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

    doi: 10.1111/j.1365-2958.2010.07474.x

    Figure Lengend Snippet: DNA inversion in the UU172 element of U. parvum serotype 3 clonal variants 27815 T -K5 and M14-K16. Schematic illustration of the DNA inversion event in (A) 27815 T -K5 and (B) M14-K16. Southern blot analyses of (A′) 27815 T -K5 before (P0) and after (P3) selective pressure with Pab α-U172C and (B′) M14-K16 before and after selective pressure with Pab α-U144. Genomic DNA was digested with BglII and HincII and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots.

    Article Snippet: Genomic DNA derived from DR1-K1 and M14-K16 was digested with 50 U of BglII (Fermentas Life Science) and 50 U of HincII (New England BioLabs) overnight at 37°C.

    Techniques: Southern Blot

    DNA inversion of the UU172 element in U. parvum serotype 3 clonal variant V892-K2. A and B. (A) Schematic illustration of the DNA inversion event and (B) Southern blot analysis of V892-K2 before (P0) and after (P3) selective pressure with Pab α-U172C. Genomic DNA was digested with BglII and EcoRV and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots. C. PCR analysis of subclones A and B with primer pairs 17/20, 17/22, 23/24 and 23/25 (see Table S3 ).

    Journal: Molecular Microbiology

    Article Title: Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172

    doi: 10.1111/j.1365-2958.2010.07474.x

    Figure Lengend Snippet: DNA inversion of the UU172 element in U. parvum serotype 3 clonal variant V892-K2. A and B. (A) Schematic illustration of the DNA inversion event and (B) Southern blot analysis of V892-K2 before (P0) and after (P3) selective pressure with Pab α-U172C. Genomic DNA was digested with BglII and EcoRV and hybridized with the DIG-11-dUTP-labelled probes (see Table S3 ) indicated below the blots. C. PCR analysis of subclones A and B with primer pairs 17/20, 17/22, 23/24 and 23/25 (see Table S3 ).

    Article Snippet: Genomic DNA derived from DR1-K1 and M14-K16 was digested with 50 U of BglII (Fermentas Life Science) and 50 U of HincII (New England BioLabs) overnight at 37°C.

    Techniques: Variant Assay, Southern Blot, Polymerase Chain Reaction

    Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. pTW423 is digested with BglII and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.

    Journal: Molecular and Cellular Biology

    Article Title: Rejoining of DNA Double-Strand Breaks as a Function of Overhang Length †

    doi: 10.1128/MCB.25.3.896-906.2005

    Figure Lengend Snippet: Oligonucleotide-modified plasmid assay. (A) Plasmid modification scheme. pTW423 is digested with BglII and XhoI and purified, removing the polyterminator. Annealed oligonucleotides are then ligated onto the BglII and XhoI ends, restoring the ADE2 coding sequence. Precise in-frame repair of the break yields Ade + colonies. (B) Primer extension assay to determine the oligonucleotide ligation efficiency. Annealed oligonucleotides (Oligos) were added to the ligation reaction mixture at concentrations of 50, 100, 500, 1,000, and 5,000-fold molar excess over the concentration of the linearized plasmid (indicated by the thickness of the triangle over the lanes). Primer extension was performed after ligation as described in Materials and Methods.

    Article Snippet: Annealed oligonucleotides (200 pmol) were ligated onto 4 pmol of pTW423 digested with BglII and XhoI with T4 DNA ligase (Roche Molecular Biochemicals) at 16°C for 16 h. Plasmids were then purified from unligated oligonucleotides with the GeneClean kit (QBioGene).

    Techniques: Modification, Plasmid Preparation, Purification, Sequencing, Primer Extension Assay, Ligation, Concentration Assay

    Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.

    Journal: Frontiers in Pharmacology

    Article Title: Lotus Leaf Aqueous Extract Reduces Visceral Fat Mass and Ameliorates Insulin Resistance in HFD-Induced Obese Rats by Regulating PPARγ2 Expression

    doi: 10.3389/fphar.2017.00409

    Figure Lengend Snippet: Verification of pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid by electrophoresis. Lane 1: DNA marker DL2000. Lane 2: Inserting fragment from pGL3-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (625 bp). Lane 3: Vector fragment from pGL3-Enhancer-Luc digested by KpnI and BglII (5064 bp). Lane 4: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc plasmid (5689 bp). Lane 5: The constructed pGL3-Enhancer-PPARγ2 (625 bp)-Luc digested by KpnI and BglII (5064/625 bp). Lane 6: DNA marker III.

    Article Snippet: In brief, pGL3-PPARγ2 (625 bp)-Luc, which contained the human PPARγ2 gene 5′-promoter fragment spanning -615 to +10 bp (+1 indicates the transcription start site) and was constructed previously in our laboratory, was digested by the restriction enzymes KpnI and BglII (Takara, Japan) to get the 625 bp PPARγ2 gene promoter inserting fragment.

    Techniques: Plasmid Preparation, Electrophoresis, Marker, Construct