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    New England Biolabs bgl ii
    Bgl Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1676 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bgl ii restriction enzyme
    Southern blot analysis of the region flanked by LCRs K1 and K2 at the Xq28 chromosome LCRs K1 and K2 are approximately 11.3 kb in length, and are located ~38 kb apart in inverted orientation. Their 99% nucleotide sequence identity is likely maintained by frequent gene conversion 34 . These LCRs flank two genes FLNA and EMD . (a) Yellow arrows indicate an inversion: cent- FLNA/EMD -tel (H1) and the alternative genomic orientation cent- EMD/FLNA -tel (H2). An 18.2 kb band is expected to be produced in either inversion haplotype background (H1dup or H2dup) upon duplication and inversion; we hypothesize that the 18.2 kb band includes the breakpoint junction 1 ( jct1 ). To test the haplotype of our cohort and to map the duplication breakpoints, we performed a Southern blot assay as shown here. Genomic <t>DNA</t> was digested with <t>Bgl</t> II; EMD was targeted using a PCR-based probe. The reference genome H1 produces a 30.7 kb band whereas the inversion haplotype (H2) yields an 18.2 kb band. (b) Southern blot results for patients carrying triplications embedded within duplications: BAB2769, BAB2772, BAB2796/BAB2980, BAB2797, BAB2801, BAB2805 (left) and family HOU1217 (right). NA10851: male carrying the reference haplotype (H1); NA15510 heterozygous female carrying both reference and inversion haplotypes (H1 and H2). BAB2771: patient carrying MECP2 duplication not involving LCR K1 and LCR K2 (H1).
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    New England Biolabs restriction enzyme bgl ii
    Southern blot hybridisation to localise <t>pKS-VOTL</t> plasmid in MS-H transformants. (A) Schematic presentation for the integration event of pKS-VOTL plasmid into genomic DNA of MS-H. A single putative homologous recombination event between the oriC copy carried by plasmid and chromosomal oriC region is represented by crossed lines. P indicates the promoter region of vlhA gene. (B, C, and D) <t>Bgl</t> II digested DNA of pKS-VOTL, untransformed MS-H and pKS-VOTL MS-H transformants MS-H-T75, MS-H-T78, MS-H-T90 (at passage 4 th and 8 th ) was hybridised with DIG labeled tetM (B), oriC (C) and vlhA - obg probes (D).
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    Southern blot hybridisation to localise <t>pKS-VOTL</t> plasmid in MS-H transformants. (A) Schematic presentation for the integration event of pKS-VOTL plasmid into genomic DNA of MS-H. A single putative homologous recombination event between the oriC copy carried by plasmid and chromosomal oriC region is represented by crossed lines. P indicates the promoter region of vlhA gene. (B, C, and D) <t>Bgl</t> II digested DNA of pKS-VOTL, untransformed MS-H and pKS-VOTL MS-H transformants MS-H-T75, MS-H-T78, MS-H-T90 (at passage 4 th and 8 th ) was hybridised with DIG labeled tetM (B), oriC (C) and vlhA - obg probes (D).
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    New England Biolabs bgl ii digested pcambia 0380
    Southern blot hybridisation to localise <t>pKS-VOTL</t> plasmid in MS-H transformants. (A) Schematic presentation for the integration event of pKS-VOTL plasmid into genomic DNA of MS-H. A single putative homologous recombination event between the oriC copy carried by plasmid and chromosomal oriC region is represented by crossed lines. P indicates the promoter region of vlhA gene. (B, C, and D) <t>Bgl</t> II digested DNA of pKS-VOTL, untransformed MS-H and pKS-VOTL MS-H transformants MS-H-T75, MS-H-T78, MS-H-T90 (at passage 4 th and 8 th ) was hybridised with DIG labeled tetM (B), oriC (C) and vlhA - obg probes (D).
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    Thermo Fisher bgl ii restriction enzymes
    Southern blot hybridisation to localise <t>pKS-VOTL</t> plasmid in MS-H transformants. (A) Schematic presentation for the integration event of pKS-VOTL plasmid into genomic DNA of MS-H. A single putative homologous recombination event between the oriC copy carried by plasmid and chromosomal oriC region is represented by crossed lines. P indicates the promoter region of vlhA gene. (B, C, and D) <t>Bgl</t> II digested DNA of pKS-VOTL, untransformed MS-H and pKS-VOTL MS-H transformants MS-H-T75, MS-H-T78, MS-H-T90 (at passage 4 th and 8 th ) was hybridised with DIG labeled tetM (B), oriC (C) and vlhA - obg probes (D).
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    Image Search Results


    Southern blot analysis of the region flanked by LCRs K1 and K2 at the Xq28 chromosome LCRs K1 and K2 are approximately 11.3 kb in length, and are located ~38 kb apart in inverted orientation. Their 99% nucleotide sequence identity is likely maintained by frequent gene conversion 34 . These LCRs flank two genes FLNA and EMD . (a) Yellow arrows indicate an inversion: cent- FLNA/EMD -tel (H1) and the alternative genomic orientation cent- EMD/FLNA -tel (H2). An 18.2 kb band is expected to be produced in either inversion haplotype background (H1dup or H2dup) upon duplication and inversion; we hypothesize that the 18.2 kb band includes the breakpoint junction 1 ( jct1 ). To test the haplotype of our cohort and to map the duplication breakpoints, we performed a Southern blot assay as shown here. Genomic DNA was digested with Bgl II; EMD was targeted using a PCR-based probe. The reference genome H1 produces a 30.7 kb band whereas the inversion haplotype (H2) yields an 18.2 kb band. (b) Southern blot results for patients carrying triplications embedded within duplications: BAB2769, BAB2772, BAB2796/BAB2980, BAB2797, BAB2801, BAB2805 (left) and family HOU1217 (right). NA10851: male carrying the reference haplotype (H1); NA15510 heterozygous female carrying both reference and inversion haplotypes (H1 and H2). BAB2771: patient carrying MECP2 duplication not involving LCR K1 and LCR K2 (H1).

    Journal: Nature Genetics

    Article Title: Inverted genomic segments and complex triplication rearrangements are mediated by inverted repeats in the human genome

    doi: 10.1038/ng.944

    Figure Lengend Snippet: Southern blot analysis of the region flanked by LCRs K1 and K2 at the Xq28 chromosome LCRs K1 and K2 are approximately 11.3 kb in length, and are located ~38 kb apart in inverted orientation. Their 99% nucleotide sequence identity is likely maintained by frequent gene conversion 34 . These LCRs flank two genes FLNA and EMD . (a) Yellow arrows indicate an inversion: cent- FLNA/EMD -tel (H1) and the alternative genomic orientation cent- EMD/FLNA -tel (H2). An 18.2 kb band is expected to be produced in either inversion haplotype background (H1dup or H2dup) upon duplication and inversion; we hypothesize that the 18.2 kb band includes the breakpoint junction 1 ( jct1 ). To test the haplotype of our cohort and to map the duplication breakpoints, we performed a Southern blot assay as shown here. Genomic DNA was digested with Bgl II; EMD was targeted using a PCR-based probe. The reference genome H1 produces a 30.7 kb band whereas the inversion haplotype (H2) yields an 18.2 kb band. (b) Southern blot results for patients carrying triplications embedded within duplications: BAB2769, BAB2772, BAB2796/BAB2980, BAB2797, BAB2801, BAB2805 (left) and family HOU1217 (right). NA10851: male carrying the reference haplotype (H1); NA15510 heterozygous female carrying both reference and inversion haplotypes (H1 and H2). BAB2771: patient carrying MECP2 duplication not involving LCR K1 and LCR K2 (H1).

    Article Snippet: We digested DNA with Bgl II restriction enzyme for 1 day at 37°C (New England Biolabs), followed by separation on a 0.7% agarose gel in 0.5X Tris–Borate–EDTA buffer.

    Techniques: Southern Blot, Sequencing, Produced, Polymerase Chain Reaction

    Southern blot hybridisation to localise pKS-VOTL plasmid in MS-H transformants. (A) Schematic presentation for the integration event of pKS-VOTL plasmid into genomic DNA of MS-H. A single putative homologous recombination event between the oriC copy carried by plasmid and chromosomal oriC region is represented by crossed lines. P indicates the promoter region of vlhA gene. (B, C, and D) Bgl II digested DNA of pKS-VOTL, untransformed MS-H and pKS-VOTL MS-H transformants MS-H-T75, MS-H-T78, MS-H-T90 (at passage 4 th and 8 th ) was hybridised with DIG labeled tetM (B), oriC (C) and vlhA - obg probes (D).

    Journal: PLoS ONE

    Article Title: Complementation of the Mycoplasma synoviae MS-H vaccine strain with wild-type obg influencing its growth characteristics

    doi: 10.1371/journal.pone.0194528

    Figure Lengend Snippet: Southern blot hybridisation to localise pKS-VOTL plasmid in MS-H transformants. (A) Schematic presentation for the integration event of pKS-VOTL plasmid into genomic DNA of MS-H. A single putative homologous recombination event between the oriC copy carried by plasmid and chromosomal oriC region is represented by crossed lines. P indicates the promoter region of vlhA gene. (B, C, and D) Bgl II digested DNA of pKS-VOTL, untransformed MS-H and pKS-VOTL MS-H transformants MS-H-T75, MS-H-T78, MS-H-T90 (at passage 4 th and 8 th ) was hybridised with DIG labeled tetM (B), oriC (C) and vlhA - obg probes (D).

    Article Snippet: Genomic DNA from pKS-VOTL MS-H transformants, untransformed MS-H and pKS-VOTL plasmid was digested with restriction enzyme Bgl II (New England Biolabs, Wilbury Way, Hitchin, England) according to manufacturer’s recommendations.

    Techniques: Southern Blot, Hybridization, Plasmid Preparation, Mass Spectrometry, Homologous Recombination, Labeling