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    Millipore beta hexaclorocyclohexane β hch
    Evaluation of the signaling pathways triggered by <t>β-hexaclorocyclohexane</t> <t>(β-HCH).</t> Immunoblot analysis of the time-course assay performed on human prostate cancer (LNCaP), human breast cancer (MCF-7 and MDA-MB 468), and human hepatoma (HepG2) cells treated with 10 μM β-HCH. Samples were analyzed for signal transducer and activator of transcription 3 (STAT3) and each cell line for a specific membrane or membrane associated tyrosine kinase receptor: EGFR in MDA-MB 468 cells, JAK2 in HepG2 cells, SRC in LNCaP cells and HER2 in MCF-7 cells. Both unmodified and the corresponding phosphorylated form were detected for each protein using specific antibodies.
    Beta Hexaclorocyclohexane β Hch, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 18 article reviews
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    beta hexaclorocyclohexane β hch - by Bioz Stars, 2020-08
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    Evaluation of the signaling pathways triggered by β-hexaclorocyclohexane (β-HCH). Immunoblot analysis of the time-course assay performed on human prostate cancer (LNCaP), human breast cancer (MCF-7 and MDA-MB 468), and human hepatoma (HepG2) cells treated with 10 μM β-HCH. Samples were analyzed for signal transducer and activator of transcription 3 (STAT3) and each cell line for a specific membrane or membrane associated tyrosine kinase receptor: EGFR in MDA-MB 468 cells, JAK2 in HepG2 cells, SRC in LNCaP cells and HER2 in MCF-7 cells. Both unmodified and the corresponding phosphorylated form were detected for each protein using specific antibodies.

    Journal: International Journal of Molecular Sciences

    Article Title: STAT3, a Hub Protein of Cellular Signaling Pathways, Is Triggered by β-Hexaclorocyclohexane

    doi: 10.3390/ijms19072108

    Figure Lengend Snippet: Evaluation of the signaling pathways triggered by β-hexaclorocyclohexane (β-HCH). Immunoblot analysis of the time-course assay performed on human prostate cancer (LNCaP), human breast cancer (MCF-7 and MDA-MB 468), and human hepatoma (HepG2) cells treated with 10 μM β-HCH. Samples were analyzed for signal transducer and activator of transcription 3 (STAT3) and each cell line for a specific membrane or membrane associated tyrosine kinase receptor: EGFR in MDA-MB 468 cells, JAK2 in HepG2 cells, SRC in LNCaP cells and HER2 in MCF-7 cells. Both unmodified and the corresponding phosphorylated form were detected for each protein using specific antibodies.

    Article Snippet: Beta-hexaclorocyclohexane (β-HCH) (Sigma-Aldrich, 33376), at a final concentration of 10 μM, was tested on each cell line pre-treated or not with specific inhibitors: 6 μM AZD1480 (Sigma-Aldrich, SML1505), 100 μM S3I-201 (Sigma-Aldrich, SML0330), 70 nM Dasatinib (Selleckchem, Roma, Italy, Cat. No. S1021), 0.8 μM Lapatinib (Sigma-Aldrich, CDS022971), and 15 μM Gefitinib (Sigma-Aldrich, SLM1657).

    Techniques: Multiple Displacement Amplification

    Computational results of hexachlorocyclohexane (HCH) binding to bases. Representative image of the gradient isosurface (left), the corresponding plots of reduced density gradient versus the sign of the second Hessian eigenvalues (right) of a adenine–α-HCH, b cytosine–α-HCH, c guanine–α-HCH, d thymine–α-HCH, together with the molecular orbitals of e DNA bases–α-HCH, f DNA bases–β-HCH, and g DNA bases–γ-HCH. The surfaces are colored on a blue–green–red scale according to the sign( λ 2 ) ρ values (range −0.05 to 0.05 a.u.). Green areas between molecules indicate a weak Van der Waals force. Large brown and olive spheres represent the positive and negative phases, respectively, of the electronic wave function

    Journal: Communications Biology

    Article Title: Organochlorinated pesticides expedite the enzymatic degradation of DNA

    doi: 10.1038/s42003-019-0326-5

    Figure Lengend Snippet: Computational results of hexachlorocyclohexane (HCH) binding to bases. Representative image of the gradient isosurface (left), the corresponding plots of reduced density gradient versus the sign of the second Hessian eigenvalues (right) of a adenine–α-HCH, b cytosine–α-HCH, c guanine–α-HCH, d thymine–α-HCH, together with the molecular orbitals of e DNA bases–α-HCH, f DNA bases–β-HCH, and g DNA bases–γ-HCH. The surfaces are colored on a blue–green–red scale according to the sign( λ 2 ) ρ values (range −0.05 to 0.05 a.u.). Green areas between molecules indicate a weak Van der Waals force. Large brown and olive spheres represent the positive and negative phases, respectively, of the electronic wave function

    Article Snippet: Chemicals Salmon sperm DNA with an average molar mass of 1.3 × 106 Da and %G-C content of 41.2%, α-HCH, β-HCH, and γ-HCH were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques: Binding Assay

    Structural changes in DNA and stability of DNA–hexachlorocyclohexane (HCH) complexes. a Circular dichroism spectra of DNA (black line), α-HCH–DNA (red line), β-HCH–DNA (olive line), and γ-HCH–DNA (blue line). Red arrow indicates a shift in molar ellipticity. b Optimized DNA–HCH structure. c RMSD over 80,000 ps α-HCH–DNA (red line), β-HCH–DNA (olive line), and γ-HCH–DNA (blue line)

    Journal: Communications Biology

    Article Title: Organochlorinated pesticides expedite the enzymatic degradation of DNA

    doi: 10.1038/s42003-019-0326-5

    Figure Lengend Snippet: Structural changes in DNA and stability of DNA–hexachlorocyclohexane (HCH) complexes. a Circular dichroism spectra of DNA (black line), α-HCH–DNA (red line), β-HCH–DNA (olive line), and γ-HCH–DNA (blue line). Red arrow indicates a shift in molar ellipticity. b Optimized DNA–HCH structure. c RMSD over 80,000 ps α-HCH–DNA (red line), β-HCH–DNA (olive line), and γ-HCH–DNA (blue line)

    Article Snippet: Chemicals Salmon sperm DNA with an average molar mass of 1.3 × 106 Da and %G-C content of 41.2%, α-HCH, β-HCH, and γ-HCH were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques:

    Gel electrophoresis of DNA fragments. a α-HCH, b β-HCH, and c γ-HCH (0–4.0 mg L −1 ). a, b, c, d, e, f, g, h, and i represent hexachlorocyclohexane (HCH) concentrations of 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg L −1 , respectively. Ck, control treatment without DNase I

    Journal: Communications Biology

    Article Title: Organochlorinated pesticides expedite the enzymatic degradation of DNA

    doi: 10.1038/s42003-019-0326-5

    Figure Lengend Snippet: Gel electrophoresis of DNA fragments. a α-HCH, b β-HCH, and c γ-HCH (0–4.0 mg L −1 ). a, b, c, d, e, f, g, h, and i represent hexachlorocyclohexane (HCH) concentrations of 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 mg L −1 , respectively. Ck, control treatment without DNase I

    Article Snippet: Chemicals Salmon sperm DNA with an average molar mass of 1.3 × 106 Da and %G-C content of 41.2%, α-HCH, β-HCH, and γ-HCH were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques: Nucleic Acid Electrophoresis

    Increase of DNA absorbance caused by DNase I in the presence of hexachlorocyclohexanes (HCHs). a The DNA absorbance changes influenced by α-HCH. b The DNA absorbance changes influenced by β-HCH. c The DNA absorbance changes influenced by γ-HCH. Each absorbance data point was the average of 10 measurements

    Journal: Communications Biology

    Article Title: Organochlorinated pesticides expedite the enzymatic degradation of DNA

    doi: 10.1038/s42003-019-0326-5

    Figure Lengend Snippet: Increase of DNA absorbance caused by DNase I in the presence of hexachlorocyclohexanes (HCHs). a The DNA absorbance changes influenced by α-HCH. b The DNA absorbance changes influenced by β-HCH. c The DNA absorbance changes influenced by γ-HCH. Each absorbance data point was the average of 10 measurements

    Article Snippet: Chemicals Salmon sperm DNA with an average molar mass of 1.3 × 106 Da and %G-C content of 41.2%, α-HCH, β-HCH, and γ-HCH were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques: