Journal: Scientific Reports

Article Title: Polyphyllin I suppresses human osteosarcoma growth by inactivation of Wnt/β-catenin pathway in vitro and in vivo

doi: 10.1038/s41598-017-07194-9

Figure Lengend Snippet: PPI suppressed osteosarcoma cells by specifically inactivating Wnt/β-catenin signaling pathway. ( A ) RT-PCR analysis of β-catenin expression level in matched human osteosarcoma tissues (tumors) and adjacent noncancerous tissues (normal) from 3 patients. ( B ) 143-B cells and ( C ) HOS cells were respectively treated with 0.8 μM PPI for indicated times, and expressions of test proteins were examined by western blotting analysis, β-actin was used as loading control, and the full-length blots were included in the supplementary information file as Figures and . 143-B cells were pretreated with 4 μM CHIR9902 (the specific GSK-3β inhibitor) for 24 h before exposed to 0.8 μM PPI for another 48 h, the combined CHIR and PPI treatments result in rescued ( D ) active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ) and cell viability ( E ) compared to the PPI treatment alone in 143-B osteosarcoma cells. 143-B cells were transfected with either small interfering RNA-targeting β-catenin (si-β-catenin) or si-control for 48 h before exposed to 0.8 μM PPI for another 24 h, si-β-catenin potentiated PPI induced ( F ) down-regulation of active β-catenin expression (the full-length blots were included in the supplementary information file as Figure ), ( G ) decrease of cell viability and ( H ) inhibition of migration of 143-B osteosarcoma cells induced by PPI. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the control.

Article Snippet: Briefly, 143-B cells were co-transfected with either small interfering RNA-targeting β-catenin (100 nM si-β-catenin) or 100 nM si-control for 72 h, using Lipofectamine 2000 (Invitrogen). β-catenin siRNA II (#6238) was bought from Cell Signaling Technology (Danvers, USA). si-control was designed and produced by Shanghai GenePharma Co.,Ltd (Shanghai, China).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Transfection, Small Interfering RNA, Inhibition, Migration

Journal: Oncology Letters

Article Title: Inhibitory effects of XAV939 on the proliferation of small-cell lung cancer H446 cells and Wnt/β-catenin signaling pathway in vitro

doi: 10.3892/ol.2018.8790

Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction assay of (A) β-catenin and (B) cyclin D1 expression. H446 cells were treated with different concentrations of XAV939 for 24 h. The expression of β-catenin and cyclin D1 compared with the blank control. The expression of β-catenin and cyclin D1 is negatively regulated by XAV939. *P<0.05 vs. control.

Article Snippet: The membranes were then incubated with the following primary antibodies: Anti-β-catenin (cat no. 6387; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-cyclin D1 (cat no. 2922; Cell Signaling Technology, Inc.) and anti-β-actin (cat no. AF0003; Beyotime Institute of Biotechnology) at a dilution of 1:1,000 at 4°C overnight.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Oncology Letters

Article Title: Inhibitory effects of XAV939 on the proliferation of small-cell lung cancer H446 cells and Wnt/β-catenin signaling pathway in vitro

doi: 10.3892/ol.2018.8790

Figure Lengend Snippet: H446 cells were treated with different concentrations of XAV939 for 24 h. (A) Western blotting analysis of β-catenin and cyclin D1 expression in H446 cells compared with β-actin. The expression of (B) β-catenin and (C) cyclin D1 is inhibited by XAV939 compared with the blank control. Each bar represents the mean ± standard deviation from three independent experiments. *P<0.05.

Article Snippet: The membranes were then incubated with the following primary antibodies: Anti-β-catenin (cat no. 6387; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-cyclin D1 (cat no. 2922; Cell Signaling Technology, Inc.) and anti-β-actin (cat no. AF0003; Beyotime Institute of Biotechnology) at a dilution of 1:1,000 at 4°C overnight.

Techniques: Western Blot, Expressing, Standard Deviation